Article ; Online: Structures and Dynamics of β-Rich Oligomers of ATTR (105-115) Assembly.
ACS chemical neuroscience
2024 Volume 15, Issue 7, Page(s) 1356–1365
Abstract: Transthyretin (TTR) is a tetrameric homologous protein that can dissociate into monomers. Misfolding and aggregation of TTR can lead to amyloid transthyretin amyloidosis (ATTR), which can cause many diseases (e.g., senile systemic amyloidosis, familial ... ...
| Abstract | Transthyretin (TTR) is a tetrameric homologous protein that can dissociate into monomers. Misfolding and aggregation of TTR can lead to amyloid transthyretin amyloidosis (ATTR), which can cause many diseases (e.g., senile systemic amyloidosis, familial amyloid cardiomyopathy, and familial amyloid polyneuropathy). Despite growing evidence indicating that small oligomers play a critical role in regulating cytotoxicity, the structures of these oligomeric intermediates and their conformational transformations are still unclear, impeding our understanding of neurodegenerative mechanisms and the development of therapeutics targeting early aggregation species. The TTR monomer protein consists of various fragments prone to self-aggregation, including the residue 105-115 sequence. Therefore, our study investigated the assembly progress of ATTR (105-115) peptides using all-atom molecular dynamics simulations. The findings indicate that the probability of β-sheet content increases with increasing numbers of peptides. Additionally, interactions between hydrophobic residues L110 and L111 are crucial for the formation of a β-rich oligomer formation. These β-rich oligomers may adopt β-barrel conformations, potentially toxic oligomer species. Free-energy analysis reveals that β-barrel conformations serve as intermediates for these β-rich oligomers. Our insights into the structural ensemble dynamics of ATTR (105-115) contribute to understanding the physical mechanisms underlying the β-barrel oligomers of ATTR. These findings may shed light on the pathological role of ATTR in neurodegenerative diseases and offer potential therapeutic targets. |
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| MeSH term(s) | Prealbumin ; Amyloid/metabolism ; Molecular Dynamics Simulation ; Amyloidogenic Proteins ; Peptides/chemistry ; Entropy ; Amyloid Neuropathies, Familial |
| Chemical Substances | Prealbumin ; Amyloid ; Amyloidogenic Proteins ; Peptides |
| Language | English |
| Publishing date | 2024-03-14 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1948-7193 |
| ISSN (online) | 1948-7193 |
| DOI | 10.1021/acschemneuro.3c00574 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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