Article ; Online: Hemopexin dosing improves cardiopulmonary dysfunction in murine sickle cell disease.
Free radical biology & medicine
2021 Volume 175, Page(s) 95–107
Abstract: Hemopexin (Hpx) is a crucial defense protein against heme liberated from degraded hemoglobin during hemolysis. High heme stress creates an imbalance in Hpx bioavailability, favoring heme accumulation and downstream pathophysiological responses leading to ...
| Abstract | Hemopexin (Hpx) is a crucial defense protein against heme liberated from degraded hemoglobin during hemolysis. High heme stress creates an imbalance in Hpx bioavailability, favoring heme accumulation and downstream pathophysiological responses leading to cardiopulmonary disease progression in sickle cell disease (SCD) patients. Here, we evaluated a model of murine SCD, which was designed to accelerate red blood cell sickling, pulmonary hypertension, right ventricular dysfunction, and exercise intolerance by exposure of the mice to moderate hypobaric hypoxia. The sequence of pathophysiology in this model tracks with circulatory heme accumulation, lipid oxidation, extensive remodeling of the pulmonary vasculature, and fibrosis. We hypothesized that Hpx replacement for an extended period would improve exercise tolerance measured by critical speed as a clinically meaningful therapeutic endpoint. Further, we sought to define the effects of Hpx on upstream cardiopulmonary function, histopathology, and tissue oxidation. Our data shows that tri-weekly administrations of Hpx for three months dose-dependently reduced heme exposure and pulmonary hypertension while improving cardiac pressure-volume relationships and exercise tolerance. Furthermore, Hpx administration dose-dependently attenuated pulmonary fibrosis and oxidative modifications in the lung and myocardium of the right ventricle. Observations in our SCD murine model are consistent with pulmonary vascular and right ventricular pathology at autopsy in SCD patients having suffered from severe pulmonary hypertension, right ventricular dysfunction, and sudden cardiac death. This study provides a translational evaluation supported by a rigorous outcome analysis demonstrating therapeutic proof-of-concept for Hpx replacement in SCD. |
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| MeSH term(s) | Anemia, Sickle Cell/drug therapy ; Animals ; Heme ; Hemoglobins ; Hemolysis ; Hemopexin ; Humans ; Mice |
| Chemical Substances | Hemoglobins ; Heme (42VZT0U6YR) ; Hemopexin (9013-71-2) |
| Language | English |
| Publishing date | 2021-08-31 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 807032-5 |
| ISSN | 1873-4596 ; 0891-5849 |
| ISSN (online) | 1873-4596 |
| ISSN | 0891-5849 |
| DOI | 10.1016/j.freeradbiomed.2021.08.238 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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