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Article: Bioremediation potential of glyphosate-degrading microorganisms in eutrophicated Ecuadorian water bodies.

Hernández-Alomia, Fernanda / Ballesteros, Isabel / Castillejo, Pablo

2021 Volume 29, Issue 3, Page(s) 1550–1558

Abstract: Phosphonate compounds are the basis of many xenobiotic pollutants, such as Glyphosate (N-(phosphonomethyl-glycine). Only procaryotic microorganisms and the lower eukaryotes are capable of phosphonate biodegradation through C-P lyase pathways. Thus, the ... ...

Abstract	Phosphonate compounds are the basis of many xenobiotic pollutants, such as Glyphosate (N-(phosphonomethyl-glycine). Only procaryotic microorganisms and the lower eukaryotes are capable of phosphonate biodegradation through C-P lyase pathways. Thus, the aim of this study was to determine the presence of C-P lyase genes in Ecuadorian freshwater systems as a first step towards assessing the presence of putative glyphosate degraders. To that end, two Nested PCR assays were designed to target the gene that codifies for the subunit J (
Language	English
Publishing date	2021-11-12
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2021.11.013
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Article: Application of real-time PCR for tree nut allergen detection in processed foods

Linacero, Rosario / Sanchiz, Africa / Ballesteros, Isabel / Cuadrado, Carmen

Critical reviews in food science and nutrition. 2020 Apr. 11, v. 60, no. 7

2020

Abstract: Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized ... ...

Abstract	Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
Keywords	DNA ; allergenicity ; allergens ; analytical methods ; food allergies ; food processing ; health effects assessments ; heat treatment ; ingredients ; nuts ; processed foods ; quantitative polymerase chain reaction
Language	English
Dates of publication	2020-0411
Size	p. 1077-1093.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	1037504-1
ISSN	1549-7852 ; 1040-8398
ISSN (online)	1549-7852
ISSN	1040-8398
DOI	10.1080/10408398.2018.1557103
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Article ; Online: Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador.

Hernández-Alomía, Fernanda / Bastidas-Caldes, Carlos / Ballesteros, Isabel / Tenea, Gabriela N / Jarrín-V, Pablo / Molina, C Alfonso / Castillejo, Pablo

International journal of environmental research and public health

2023 Volume 20, Issue 3

Abstract: Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive ... ...

Abstract	Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through
MeSH term(s)	Humans ; Ecuador ; beta-Lactamases/genetics ; beta-Lactamases/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacteria/metabolism ; Monobactams ; Drug Resistance, Multiple, Bacterial ; Microbial Sensitivity Tests
Chemical Substances	beta-Lactamases (EC 3.5.2.6) ; Anti-Bacterial Agents ; Monobactams
Language	English
Publishing date	2023-01-20
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20031900
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Article ; Online: Application of real-time PCR for tree nut allergen detection in processed foods.

Linacero, Rosario / Sanchiz, Africa / Ballesteros, Isabel / Cuadrado, Carmen

Critical reviews in food science and nutrition

2019 Volume 60, Issue 7, Page(s) 1077–1093

Abstract	Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
MeSH term(s)	Allergens/analysis ; Allergens/genetics ; Allergens/immunology ; Fast Foods/analysis ; Food Hypersensitivity/prevention & control ; Humans ; Nuts/chemistry ; Nuts/immunology ; Real-Time Polymerase Chain Reaction
Chemical Substances	Allergens
Language	English
Publishing date	2019-01-13
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1037504-1
ISSN	1549-7852 ; 1040-8398
ISSN (online)	1549-7852
ISSN	1040-8398
DOI	10.1080/10408398.2018.1557103
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Article ; Online: Changes Induced by Pressure Processing on Immunoreactive Proteins of Tree Nuts.

Cuadrado, Carmen / Sanchiz, Africa / Vicente, Fatima / Ballesteros, Isabel / Linacero, Rosario

Molecules (Basel, Switzerland)

2020 Volume 25, Issue 4

Abstract: Tree nuts confer many health benefits due to their high content of vitamins and antioxidants, and they are increasingly consumed in the last few years. Food processing is an important industrial tool to modify allergenic properties of foods, in addition ... ...

Abstract	Tree nuts confer many health benefits due to their high content of vitamins and antioxidants, and they are increasingly consumed in the last few years. Food processing is an important industrial tool to modify allergenic properties of foods, in addition to ensuring safety and enhancing organoleptic characteristics. The effect of high pressure, without and with heating, on SDS-PAGE and immunodetection profile of potential allergenic proteins (anti-11S, anti-2S and anti-LTP) of pistachio, cashew, peanut, hazelnut, almond, and chestnut was investigated. Processing based on heat and/or pressure and ultra-high pressure (HHP, 300-600 MPa) without heating was applied. After treating the six tree nuts with pressure combined with heat, a progressive diminution of proteins with potential allergenic properties was observed. Moreover, some tree nuts proteins (pistachio, cashew, and peanut) seemed to be more resistant to technological processing than others (hazelnut and chestnut). High pressure combined with heating processing markedly reduce tree nut allergenic potential as the pressure and treatment time increases. HHP do not alter hazelnut and almond immunoreactivity.
MeSH term(s)	Allergens/chemistry ; Allergens/immunology ; Food Handling ; Food Hypersensitivity ; Hot Temperature ; Humans ; Nuts/chemistry ; Nuts/immunology ; Plant Proteins/chemistry ; Plant Proteins/immunology ; Pressure
Chemical Substances	Allergens ; Plant Proteins
Language	English
Publishing date	2020-02-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules25040954
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Article: Genetic Variability of

Ballesteros, Isabel / Bravo-Castro, Mishell / Villamarín-Cortez, Santiago / Jijón, Gabriela / Prat, Narcís / Ríos-Touma, Blanca / Villamarín, Christian

Insects

2022 Volume 13, Issue 4

Abstract: Chironomids show a wide distribution and can occupy several habitats due to their high adaptive capacity in different freshwater environments. The ... ...

Abstract	Chironomids show a wide distribution and can occupy several habitats due to their high adaptive capacity in different freshwater environments. The genus
Language	English
Publishing date	2022-04-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662247-6
ISSN	2075-4450
ISSN	2075-4450
DOI	10.3390/insects13040382
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Article ; Online: Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators.

Ballesteros, Isabel / Castillejo, Pablo / Haro, Adriana Paulina / Montes, Cuthy Cristina / Heinrich, Carla / Lobo, Eduardo Alexis

Comptes rendus biologies

2020 Volume 343, Issue 1, Page(s) 41–52

Abstract: Diatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to ... ...

Abstract	Diatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to complement the diagnosis. The main goal of this work was to obtain the DNA barcode of Ecuadorian epilithic diatoms with a wide geographical distribution, a well-defined ecological range and characteristics that allow them to be reliable indicator species. Unialgal diatom cultures were obtained from environmental samples of Ecuadorian Andean streams. Morphological characterization of cultures was carried out under SEM microscopy. For molecular characterization, 18SV4 and rbcL barcodes were sequenced from each strain and blasted against a GenBank database. A phylogenetic tree for each barcode was constructed using the ML method including sequences of strains of the studied species from different geographical locations. The results showed the following five species to be suitable as bioindicators and these were isolated. Sellaphora seminulum (strain JA01b, c), Nitzschia fonticola (strain SP02a) and N. palea (strain CA01a) are tolerant to eutrophication; Eolimna minima (strain CH02a) is a mesotrophic water bioindicator, and Achnanthidium minutissimum (strain JA01a) is an oligotrophic water bioindicator. The comparison with the GenBank database of the barcoding regions supported the morphological identification. The barcoding sequences of the strains showed a high percentage of identity with the sequences reported in INSDC databases for the same species. The topology of the phylogenetic trees demonstrates that epilithic diatoms from Ecuador are closely related to those of same species isolated from other geographical regions. This study is a first attempt to establish a morphological and molecular taxonomic reference library for neotropical diatoms. This study demonstrates that it would be feasible to use the existing barcoding data for diatoms to develop molecular tools for the bioassessment of aquatic ecosystems in the Ecuadorian Andean region.
MeSH term(s)	DNA Barcoding, Taxonomic/methods ; Diatoms/classification ; Diatoms/genetics ; Ecosystem ; Ecuador ; Environmental Biomarkers ; Eutrophication ; Phylogeny ; Rivers ; Water Quality
Chemical Substances	Environmental Biomarkers
Language	English
Publishing date	2020-06-05
Publishing country	France
Document type	News
ZDB-ID	2072863-3
ISSN	1768-3238 ; 1631-0691
ISSN (online)	1768-3238
ISSN	1631-0691
DOI	10.5802/crbiol.2
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Article: Chestnut allergen detection in complex food products: Development and validation of a real-time PCR method

Sanchiz, África / Ballesteros, Isabel / López-García, Adrián / Ramírez, Ana / Rueda, Julia / Cuadrado, Carmen / Linacero, Rosario

Lebensmittel-Wissenschaft + [i.e. und] Technologie. 2020 Apr., v. 123

2020

Abstract: Chestnut is gaining importance as food allergen, but a specific detection method has not been performed so far. For the first time, the development of a real-time PCR assay for chestnut allergen detection in processed foods is described. Initially, three ...

Abstract	Chestnut is gaining importance as food allergen, but a specific detection method has not been performed so far. For the first time, the development of a real-time PCR assay for chestnut allergen detection in processed foods is described. Initially, three sets of novel primers specific for allergen-encoding genes (Cas s 5, Cas s 9 and Cas s TLP) were designed and evaluated for chestnut detection. Primers for Cas s 9 and a TaqMan probe were finally selected for a sensitive and specific detection of chestnut in binary mixtures with a practical limit of detection (LOD) of 100 ppm. Effect of processing has been evaluated and sensitivity slightly reduced after boiling treatment. Amplification of Cas s 9 was importantly affected when heat and pressure were applied (autoclaving). Applicability of the method was tested by analysis of commercial food products. Consequently, the results support the development and validation of a real-time PCR method for accurate chestnut allergen detection and labelling.
Keywords	Castanea ; autoclaving ; boiling ; detection limit ; food allergens ; genes ; heat ; processed foods ; quantitative polymerase chain reaction
Language	English
Dates of publication	2020-04
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	241369-3
ISSN	0460-1173 ; 0023-6438
ISSN	0460-1173 ; 0023-6438
DOI	10.1016/j.lwt.2020.109067
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Article: The osmotic action of sugar combined with hydrogen peroxide and bee-derived antibacterial peptide Defensin-1 is crucial for the antibiofilm activity of eucalyptus honey

Proaño, Adrián / Coello, Dayana / Villacrés-Granda, Irina / Ballesteros, Isabel / Debut, Alexis / Vizuete, Karla / Brenciani, Andrea / Álvarez-Suarez, José M

Lebensmittel-Wissenschaft + [i.e. und] Technologie. 2021 Jan., v. 136

2021

Abstract: Honey is known for its important antimicrobial activity, which is considered one of its main biological properties. This property has been associated with different factors, among which floral origin stands out and can directly affect this biological ... ...

Abstract	Honey is known for its important antimicrobial activity, which is considered one of its main biological properties. This property has been associated with different factors, among which floral origin stands out and can directly affect this biological activity. The aim of this study was to evaluate the antibiofilm activity of eucalyptus honey (EH) against two multiresistant human pathogens Staphylococcus aureus MRSA S21 and Pseudomonas aeruginosa P28, as well as to investigate the role of several honey components in its antibiofilm action. We also explored the activity of the enzyme glucose oxidase (GOX), hydrogen peroxide (H₂O₂) content, and the presence of the antimicrobial peptide bee defensin-1 (Def-1). EH was efficient in inhibiting biofilm formation and removing preformed biofilms. This activity was markedly affected after treatment with catalase and proteinase K, suggesting that antibiofilm activity is related to the presence of H₂O₂ and Def-1. Structural changes induced by the osmotic effect of honey sugar was evidenced by scanning electron microscopy, suggesting that EH's antibiofilm capacity is not dependent solely on H₂O₂ content or the presence of Def-1.
Keywords	Eucalyptus ; Pseudomonas aeruginosa ; antimicrobial peptides ; antimicrobial properties ; bees ; biofilm ; catalase ; enzyme activity ; glucose oxidase ; honey ; humans ; hydrogen peroxide ; osmotic pressure ; peptidase K ; sugars
Language	English
Dates of publication	2021-01
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	241369-3
ISSN	0460-1173 ; 0023-6438
ISSN	0460-1173 ; 0023-6438
DOI	10.1016/j.lwt.2020.110379
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Article ; Online: SARS-CoV-2 in river water: Implications in low sanitation countries.

Guerrero-Latorre, Laura / Ballesteros, Isabel / Villacrés-Granda, Irina / Granda, M Genoveva / Freire-Paspuel, Byron / Ríos-Touma, Blanca

The Science of the total environment

2020 Volume 743, Page(s) 140832

Abstract: Since the beginning of COVID-19 pandemic studies on viral shedding have reported that this virus is excreted in feces in most patients. High viral loads are found at the sewage pipeline or at the entrance of wastewater treatment plants from cities where ... ...

Abstract	Since the beginning of COVID-19 pandemic studies on viral shedding have reported that this virus is excreted in feces in most patients. High viral loads are found at the sewage pipeline or at the entrance of wastewater treatment plants from cities where the number of COVID-19 cases are significant. In Quito (Ecuador) as in many other cities worldwide, wastewater is directly discharged into natural waters. The aim of this study was to evaluate SARS-CoV-2 presence in urban streams from a low sanitation context. Three river locations along the urban rivers of Quito were sampled on the 5th of June during a peak of COVID-19 cases. River samples were evaluated for water quality parameters and afterwards, concentrated for viral analysis using skimmed milk flocculation method. The viral concentrates were quantified for SARS-CoV-2 (N1 and N2 target regions) and Human Adenovirus as a human viral indicator. The results showed that SARS-CoV-2 was detected for both target regions in all samples analyzed in a range of 2,91E+05 to 3,19E+06 GC/L for N1 and from 2,07E+05 to 2,22E+06 GC/L for N2. The high values detected in natural waters from a low sanitation region have several implications in health and ecology that should be further assessed.
MeSH term(s)	Betacoronavirus ; COVID-19 ; Cities ; Coronavirus Infections ; Ecuador ; Humans ; Pandemics ; Pneumonia, Viral ; Rivers ; SARS-CoV-2 ; Sanitation
Keywords	covid19
Language	English
Publishing date	2020-07-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2020.140832
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