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  1. Article ; Online: Anthrax toxins-producing Bacillus spp. isolated from handwashing stations during COVID-19 pandemic in Lagos, Nigeria.

    Bamidele, Tajudeen A / Odumosu, Bamidele T / Adenola, Princess T / Ameh, James / Kareem, Olaide K / Osoba, Babatunde / Ezechi, Oliver C / Salako, Babatunde L

    Journal of infection in developing countries

    2023  Volume 17, Issue 8, Page(s) 1076–1080

    Abstract: Introduction: The virulence binding factor, protective antigen (pag) and poly-D-γ-glutamate capsular (cap) genes, peculiar to Bacillus anthracis are located in the pXO1 and pXO2 plasmids which are transferable horizontally to related species called " ... ...

    Abstract Introduction: The virulence binding factor, protective antigen (pag) and poly-D-γ-glutamate capsular (cap) genes, peculiar to Bacillus anthracis are located in the pXO1 and pXO2 plasmids which are transferable horizontally to related species called "cereus group". The cereus group are usually isolated from the environmental/food samples and have been implicated in debilitating human and animal anthrax-like diseases. This study was designed to investigate the presence of the anthrax virulence genes in different Bacillus spp. isolated from handwashing facilities during COVID-19 pandemic in Lagos, Nigeria.
    Methodology: The Bacillus anthracis (OK316847), B. thuringiensis (OK316855), B. amyloliquefaciens (OK316857), B. cereus (OK316858) and B. thuringiensis (OK316859) previously isolated from rinsates and bowl water in two local government areas (LGAs) of Lagos state were further investigated by the polymerase chain reaction (PCR) amplification of the pag and cap genes using specific primers.
    Results: Bacillus anthracis and B. cereus co-harboured the two 578 bp cap and 364 bp pag genes while B. thuringiensis only harboured the cap gene. Similarly, the non-cereus B. amyloliquefaciens was found to habour the pag gene.
    Conclusions: The two anthrax toxin genes were amplified in the Bacillus spp isolated from rinsates and bowl water used in hand washing in the two study LGAs. Given that these virulence genes have a global consequence and are a potential threat to life, this study calls for an extensive surveillance, and reassessment of gene regulators and plasmid distribution among these strains in our environment.
    MeSH term(s) Animals ; Humans ; Bacillus ; Hand Disinfection ; Anthrax/epidemiology ; Anthrax/prevention & control ; Nigeria/epidemiology ; Pandemics/prevention & control ; COVID-19/epidemiology ; COVID-19/prevention & control
    Chemical Substances anthrax toxin
    Language English
    Publishing date 2023-08-31
    Publishing country Italy
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2394024-4
    ISSN 1972-2680 ; 2036-6590
    ISSN (online) 1972-2680
    ISSN 2036-6590
    DOI 10.3855/jidc.18228
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Mucoadhesive Microspheres of Maraviroc and Tenofovir Designed for Pre-Exposure Prophylaxis of HIV-1: An in vitro Assessment of the Effect on Vaginal Lactic Acid Bacteria Microflora.

    Ekama, Sabdat O / Ilomuanya, Margaret O / Azubuike, Chukwuemeka P / Bamidele, Tajudeen A / Fowora, Muinah A / Aina, Oluwagbemiga O / Ezechi, Oliver C / Igwilo, Cecilia I

    HIV/AIDS (Auckland, N.Z.)

    2021  Volume 13, Page(s) 399–413

    Abstract: Purpose: To formulate and evaluate microspheres of the antiretroviral drugs maraviroc and tenofovir intended for a candidate vaginal microbicide and assess its effect on the vaginal lactic acid bacteria microflora.: Methods: Ionic gelation technique ... ...

    Abstract Purpose: To formulate and evaluate microspheres of the antiretroviral drugs maraviroc and tenofovir intended for a candidate vaginal microbicide and assess its effect on the vaginal lactic acid bacteria microflora.
    Methods: Ionic gelation technique was used to formulate maraviroc and tenofovir microspheres with subsequent characterization. The effect of varying concentrations of the polymer, crosslinking agent and the curing time on the outcome variables viz: particle size, mucoadhesion and encapsulation efficiency were investigated. Lactic acid bacteria were isolated from the vagina of healthy women using standard microbiologic methods. The analysis of their 16S rRNA sequence data identified
    Results: The optimal maraviroc and tenofovir microspheres had particle sizes of (434.82 µm and 456.18 µm), mucoadhesion of (93.3% and 90%) and encapsulation efficiency (92.80% and 78.9%) respectively. Maraviroc release kinetics followed a zero-order model and tenofovir was released via Higuchi model. The assay of a 1 mg/mL suspension of the microspheres on the strains of
    Conclusion: The antiretroviral drugs loaded in the microspheres, had good mucoadhesion which is a potential for prolonged residence time in the vagina. The antiretroviral drugs were adequately released from the microspheres and showed efficacy against the HIV-1 BaL virus strain. There was no significant disruption in the growth of the lactic acid bacteria which constitute valuable bacteria microflora of the vagina.
    Language English
    Publishing date 2021-04-08
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2520734-9
    ISSN 1179-1373
    ISSN 1179-1373
    DOI 10.2147/HIV.S291065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A designathon to co-create community-driven HIV self-testing services for Nigerian youth: findings from a participatory event.

    Tahlil, Kadija M / Obiezu-Umeh, Chisom / Gbajabiamila, Titi / Nwaozuru, Ucheoma / Oladele, David / Musa, Adesola Z / Idigbe, Ifeoma / Okwuzu, Jane / David, Agatha N / Bamidele, Tajudeen A / Airhihenbuwa, Collins O / Rosenberg, Nora E / Tang, Weiming / Ong, Jason J / Conserve, Donaldson F / Iwelunmor, Juliet / Ezechi, Oliver / Tucker, Joseph D

    BMC infectious diseases

    2021  Volume 21, Issue 1, Page(s) 505

    Abstract: Background: Youth are at high risk for HIV, but are often left out of designing interventions, including those focused on adolescents. We organized a designathon for Nigerian youth to develop HIV self-testing (HIVST) strategies for potential ... ...

    Abstract Background: Youth are at high risk for HIV, but are often left out of designing interventions, including those focused on adolescents. We organized a designathon for Nigerian youth to develop HIV self-testing (HIVST) strategies for potential implementation in their local communities. A designathon is a problem-focused event where participants work together over a short period to create and present solutions to a judging panel.
    Methods: We organized a 72-h designathon for youth (14-24 years old) in Nigeria to design strategies to increase youth HIVST uptake. Proposals included details about HIVST kit service delivery, method of distribution, promotional strategy, and youth audience. Teams pitched their proposals to a diverse seven-member judging panel who scored proposals based on desirability, feasibility, potential impact and teamwork. We examined participants' socio-demographic characteristics and summarized themes from their HIVST proposals.
    Results: Forty-two youth on 13 teams participated in the designathon. The median team size was 3 participants (IQR: 2-4). The median age was 22.5 years (IQR: 21-24), 66.7% were male, 47.4% completed tertiary education, and 50% lived in Lagos State. Themes from proposals included HIVST integration with other health services, digital marketing and distribution approaches, and engaging students. Judges identified seven teams with exceptional HIVST proposals and five teams were supported for further training.
    Conclusions: The designathon provided a structured method for incorporating youth ideas into HIV service delivery. This approach could differentiate HIV services to be more youth-friendly in Nigeria and other settings.
    MeSH term(s) Adolescent ; Community Health Services/methods ; Delivery of Health Care ; Female ; HIV Infections/diagnosis ; Humans ; Male ; Mass Screening/methods ; Nigeria ; Self-Testing ; Young Adult
    Language English
    Publishing date 2021-05-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041550-3
    ISSN 1471-2334 ; 1471-2334
    ISSN (online) 1471-2334
    ISSN 1471-2334
    DOI 10.1186/s12879-021-06212-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Strategies for enhancing uptake of HIV self-testing among Nigerian youths: a descriptive analysis of the 4YouthByYouth crowdsourcing contest.

    Rosenberg, Nora E / Obiezu-Umeh, Chisom / Gbaja-Biamila, Titilola / Tahlil, Kadija M / Nwaozuru, Ucheoma / Oladele, David / Musa, Adesola Z / Idigbe, Ifeoma / Okwuzu, Jane / David, Agatha N / Bamidele, Tajudeen A / Tang, Weiming / Ezechi, Oliver / Tucker, Joseph D / Iwelunmor, Juliet

    BMJ innovations

    2018  Volume 7, Issue 3, Page(s) 590–596

    Abstract: Introduction: Youth are often the intended beneficiaries of HIV programs but are rarely involved in program design. Engaging youth in program design is one potential way of identifying promising approaches for HIV service delivery. The purpose of this ... ...

    Abstract Introduction: Youth are often the intended beneficiaries of HIV programs but are rarely involved in program design. Engaging youth in program design is one potential way of identifying promising approaches for HIV service delivery. The purpose of this study is to examine the feasibility of using a crowdsourcing contest to solicit ideas on ways to promote HIV self-testing (HIVST) services among Nigerian youths.
    Methods: From October-November, 2018 Nigerian youth 10-24 years old submitted ideas to a crowdsourcing contest on how to promote HIVST among their peers. Submissions were scored on feasibility, desirability, and impact, with an integer score of 1 (low) to 3 (high) in each domain. The three-domain scores were added to calculate a total score (3-9). The demographic characteristics of contestants were calculated using descriptive statistics.
    Results: Nine-hundred and three entries were received, 831 had unique valid responses, and 769 were eligible for scoring. Youth submitted ideas on paper (44.9%), Google Forms (39.4%), WhatsApp (9.6%), and email (6.1%). Participants' ages were 10-14 years (37%), 15-19 years (44%), and 20-24 years (22%).Approximately half were female (51.2%). Mean scores were 1.4/3.0 (SD=0.6) for feasibility, 1.4/3.0 (SD=0.6) for desirability, 1.2/3.0 (SD=0.5) for impact, and 4.0/9.0 (SD=1.5) overall. Eight percent of submissions had an overall score >7. A disproportionate share of these high-quality submissions came from email and Google submissions.
    Conclusion: The 4 Youth by Youth crowdsourcing contest engaged a broad audience and is a feasible way to elicit potential strategies to distribute HIVST kits to other youth. Several high-quality ideas require further evaluation.
    Language English
    Publishing date 2018-10-21
    Publishing country England
    Document type Journal Article
    ISSN 2055-642X
    ISSN 2055-642X
    DOI 10.1136/bmjinnov-2020-000556
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Molecular characterization of the circulating strains of Vibrio cholerae during 2010 cholera outbreak in Nigeria.

    Oyedeji, Kolawole S / Niemogha, Mary-Theresa / Nwaokorie, Francisca O / Bamidele, Tajudeen A / Ochoga, Michael / Akinsinde, Kehinde A / Brai, Bartholomew I / Oladele, David / Omonigbehin, Emmanuel A / Bamidele, Moses / Fesobi, Toun W / Musa, Adesola Z / Adeneye, Adeniyi K / Smith, Stella I / Ujah, Innocent A

    Journal of health, population, and nutrition

    2013  Volume 31, Issue 2, Page(s) 178–184

    Abstract: This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, ...

    Abstract This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were inoculated on thiosulphate-citrate bile salt-sucrose (TCBS), Cary-Blair transport medium and smeared on glass slides for direct examination. The Vibrio cholerae isolates were serotyped, biotyped, and characterized using PCR of the cytotoxin gene A (ctxA), wbeO1, and wbfO139 gene primer. Of the 201 samples screened, 96 were positive for V cholerae O1 (48%), with 69 (72%) positive for ctxA gene. The results from this study showed that the circulating strains of cholera in Nigeria were of Ogawa serotype, also observed in other outbreaks in Nigeria (1991, 1992, and 1996). However, the strains were of the Classical biotype and were mainly (72%) ctxA gene-positive. This current investigation has confirmed the production of cholera toxin by the circulating strains, and this could be harnessed for possible cholera vaccine production in Nigeria.
    MeSH term(s) Cholera/blood ; Cholera/epidemiology ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Disease Outbreaks/statistics & numerical data ; Humans ; Molecular Typing/methods ; Nigeria/epidemiology ; Polymerase Chain Reaction/methods ; Vibrio cholerae/classification ; Vibrio cholerae/genetics ; Vibrio cholerae/isolation & purification
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2013-03-06
    Publishing country Bangladesh
    Document type Journal Article
    ZDB-ID 2025045-9
    ISSN 2072-1315 ; 1606-0997
    ISSN (online) 2072-1315
    ISSN 1606-0997
    DOI 10.3329/jhpn.v31i2.16381
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: An assessment of the emergency response among health workers involved in the 2010 cholera outbreak in northern Nigeria.

    Oladele, David A / Oyedeji, Kolawole S / Niemogha, Mary-Theresa / Nwaokorie, Francisca / Bamidele, Moses / Musa, Adesola Z / Adeneye, Adeniyi K / Bamidele, Tajudeen A / Ochoga, Michael / Akinsinde, Kehinde A / Brai, Bartholomew I / Omonigbehin, Emmanuel A / Fesobi, Toun W / Smith, Stella I / Ujah, Innocent A

    Journal of infection and public health

    2012  Volume 5, Issue 5, Page(s) 346–353

    Abstract: Background: The 2010 cholera outbreak in northern Nigeria affected over 40,000 people, with a case fatality rate (CFR) of ≥3.75%. We assessed the emergency response of health care workers (HCWs) involved in case management.: Method: This was a cross- ... ...

    Abstract Background: The 2010 cholera outbreak in northern Nigeria affected over 40,000 people, with a case fatality rate (CFR) of ≥3.75%. We assessed the emergency response of health care workers (HCWs) involved in case management.
    Method: This was a cross-sectional study with data collected through a self-administered questionnaire. Data entry and analysis were performed using Epi info software.
    Results: A total of 56 HCWs were interviewed. The mean age was 31 years (SD±8.16 years). The majority of the HCWs (80%; n=45) were aged 18-39 years. Most were community health extension workers (60%), and 3.6% (n=2) were medical doctors. Many of the HCWs had less than 2 years of work experience (42%). Additionally, 82% of the respondents had <1 week of cholera emergency response training, and 50% of the HCWs managed >20 suspected cases of cholera per day. Although 78% of HCWs reported the practice of universal safety precautions, 32% (n=18) knew HCWs who developed symptoms of cholera during the epidemic, most of which was believed to be hospital acquired (78%). We also found that 77% (n=43) of HCWs had no access to the required emergency response supplies.
    Conclusion: Inadequate training, a lack of qualified HCWs and a limited supply of emergency response kits were reported. Therefore, the government and stakeholders should address the gaps noted to adequately control and prevent future epidemics.
    MeSH term(s) Adolescent ; Adult ; Attitude of Health Personnel ; Cholera/epidemiology ; Cross-Sectional Studies ; Disease Outbreaks ; Emergency Medical Services/methods ; Emergency Medical Services/statistics & numerical data ; Female ; Humans ; Infection Control/methods ; Infection Control/organization & administration ; Male ; Middle Aged ; Nigeria/epidemiology ; Professional Competence/statistics & numerical data ; Surveys and Questionnaires ; Young Adult
    Keywords covid19
    Language English
    Publishing date 2012-10-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2467587-8
    ISSN 1876-035X ; 1876-0341
    ISSN (online) 1876-035X
    ISSN 1876-0341
    DOI 10.1016/j.jiph.2012.06.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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