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  1. Article ; Online: Human adipose-derived stem cells preconditioned with a novel herbal formulation Jing Shi attenuate doxorubicin-induced cardiac damage.

    Hsieh, Dennis Jine-Yuan / Tsai, Bruce Chi-Kang / Barik, Parthasarathi / Shibu, Marthandam Asokan / Kuo, Chia-Hua / Kuo, Wei-Wen / Lin, Pi-Yu / Shih, Cheng-Yen / Lin, Shinn-Zong / Ho, Tsung-Jung / Huang, Chih-Yang

    Aging

    2023  Volume 15, Issue 17, Page(s) 9167–9181

    Abstract: Pathological cardiac hypertrophy is a considerable contributor to global disease burden. Chinese herbal medicine (CHM) has been used to treat cardiovascular diseases since antiquity. Enhancing stem cell-mediated recovery through CHM represents a ... ...

    Abstract Pathological cardiac hypertrophy is a considerable contributor to global disease burden. Chinese herbal medicine (CHM) has been used to treat cardiovascular diseases since antiquity. Enhancing stem cell-mediated recovery through CHM represents a promising approach for protection against doxorubicin (Dox)-induced cardiac hypertrophy. Herein, we investigated whether human adipose-derived stem cells (hADSCs) preconditioned with novel herbal formulation Jing Si (JS) improved protective ability of stem cells against doxorubicin-induced cardiac damage. The effect of JS on hADSC viability and migration capacity was determined via MTT and migration assays, respectively. Co-culture of hADSC or JS-preconditioned hADSCs with H9c2 cells was analyzed with immunoblot, flow cytometry, TUNEL staining, LC3B staining, F-actin staining, and MitoSOX staining. The
    MeSH term(s) Humans ; Animals ; Rats ; Rats, Sprague-Dawley ; Heart ; Stem Cells ; Doxorubicin/toxicity ; Cardiomegaly
    Chemical Substances Doxorubicin (80168379AG)
    Language English
    Publishing date 2023-09-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.205026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats.

    Barik, Parthasarathi / Kuo, Wei-Wen / Kuo, Chia-Hua / Hsieh, Dennis Jine-Yuan / Day, Cecilia Hsuan / Daddam, Jayasimharayalu / Chen, Michael Yu-Chih / Padma, V Vijaya / Shibu, Marthandam Asokan / Huang, Chih-Yang

    Aging

    2023  Volume 15, Issue 23, Page(s) 14019–14038

    Abstract: Aging-associated cardiovascular diseases depend on the longitudinal deterioration of stem cell dynamics. The entire mechanism behind it is not completely understood. However, many studies suggest that endocrine pathways, particularly the insulin-like ... ...

    Abstract Aging-associated cardiovascular diseases depend on the longitudinal deterioration of stem cell dynamics. The entire mechanism behind it is not completely understood. However, many studies suggest that endocrine pathways, particularly the insulin-like growth factor-1(IGF1) signaling pathway are involved in cardioprotection, especially in stem-cell treatments. Here, we investigated the role of a co-chaperone, carboxyl-terminus of Hsp70 interacting protein (CHIP) in the aspects of growth factor secretion and receptor stabilization in mesenchymal stem cells (MSCs). Briefly, we overexpressed CHIP in rat adipose-derived stem cells (rADSCs) and explored the consequences
    MeSH term(s) Animals ; Rats ; Adipocytes/metabolism ; Aging ; Hypertension ; Insulin-Like Growth Factor I/metabolism ; Receptor, IGF Type 1/metabolism ; Signal Transduction ; Stem Cells/metabolism
    Chemical Substances Insulin-Like Growth Factor I (67763-96-6) ; Receptor, IGF Type 1 (EC 2.7.10.1) ; Stub1 protein, rat (EC 2.3.2.27)
    Language English
    Publishing date 2023-12-11
    Publishing country United States
    Document type Journal Article
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.205287
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cardioprotective effects of transplanted adipose-derived stem cells under Ang II stress with Danggui administration augments cardiac function through upregulation of insulin-like growth factor 1 receptor in late-stage hypertension rats.

    Barik, Parthasarathi / Shibu, Marthandam Asokan / Hsieh, Dennis Jine-Yuan / Day, Cecilia Hsuan / Chen, Ray-Jade / Kuo, Wei-Wen / Chang, Yung-Ming / Padma, V Vijaya / Ho, Tsung-Jung / Huang, Chih-Yang

    Environmental toxicology

    2021  Volume 36, Issue 7, Page(s) 1466–1475

    Abstract: In aging hypertensive conditions, deterioration of insulin-like growth factor 1 receptor (IGF1R) cause a pathological impact on hypertensive hearts with an increased Ang II level. Recovering these adverse conditions through transplanted adipose-derived ... ...

    Abstract In aging hypertensive conditions, deterioration of insulin-like growth factor 1 receptor (IGF1R) cause a pathological impact on hypertensive hearts with an increased Ang II level. Recovering these adverse conditions through transplanted adipose-derived stem cells is a challenging approach. Moreover, Danggui, a Traditional Chinese medicine (TCM), is used for the treatment of cardioprotective effects. In this study, to evaluate whether the combined effect of MSCs and TCM can recover the cardiac function in late-stage hypertension rats. We observed that lower dose of Danggui crude extract treatment showed an increased level of cell viability with maintained stemness properties and growth rate in rat adipose-derived stem cells (rADSCs). Further, we cocultured the H9c2 cells with rADSCs and the results revealed that Danggui-treated MSCs enhanced the IGF1R expression and attenuated the hypertrophy in H9c2 cells against Ang II challenge by immunoblot and rhodamine-phalloidin staining. In addition, Danggui crude extract was also quantified and characterized by HPLC and LC-MS analysis. Furthermore, the in vivo study was performed by considering 11 months old rats (n = 7). Importantly, the oral administration of Danggui crude extract with stem cells intravenous injection in SHR-D-ADSCs group showed a combination effect to augment the cardiac function through enhancement of ejection fraction, fractional shortening, contractility function in the late-stage hypertension conditions. We have also observed a decreased apoptosis rate in the heart tissue of SHR-D-ADSCs group. Taken together, these results indicate that the combinatorial effects of Danggui crude extract and stem cell therapy enhanced cardiac function in late-stage SHR rats.
    MeSH term(s) Animals ; Hypertension ; Insulin-Like Growth Factor I ; Rats ; Rats, Inbred SHR ; Stem Cells ; Up-Regulation
    Chemical Substances Insulin-Like Growth Factor I (67763-96-6)
    Language English
    Publishing date 2021-04-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1463449-1
    ISSN 1522-7278 ; 1520-4081
    ISSN (online) 1522-7278
    ISSN 1520-4081
    DOI 10.1002/tox.23145
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2676: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Inhibition of cell death-inducing p53 target 1 through miR-210-3p overexpression attenuates reactive oxygen species and apoptosis in rat adipose-derived stem cells challenged with Angiotensin II.

    Lai, Chin-Hu / Barik, Parthasarathi / Hsieh, Dennis Jine-Yuan / Day, Cecilia Husan / Ho, Tsung-Jung / Chen, Ray-Jade / Kuo, Wei-Wen / Padma, V Vijaya / Shibu, Marthandam Asokan / Huang, Chih-Yang

    Biochemical and biophysical research communications

    2020  Volume 532, Issue 3, Page(s) 347–354

    Abstract: Hypoxic preconditioning is a well-known strategy to improve the survival and therapeutic potential of stem cells against various challenges including hemodynamic and neurohormonal modulations. However, the mechanism involved in hypoxia-induced benefits ... ...

    Abstract Hypoxic preconditioning is a well-known strategy to improve the survival and therapeutic potential of stem cells against various challenges including hemodynamic and neurohormonal modulations. However, the mechanism involved in hypoxia-induced benefits on stem cells is still ambiguous. In pathological hypertension, the elevation of the neurohormonal mediator Angiotensin II (Ang II) causes the adverse effects to stem cells. In this study, we investigate the effect and mechanism of action of short term hypoxia-inducible miRNA in suppressing the effects of AngII on stem cells. According to the results obtained, Ang II affects the normal cell cycle and triggers apoptosis in rADSCs with a corresponding increase in the expression of cell death-inducing p53 target 1 (CDIP1) protein. However, the short term hypoxia-inducible miRNA-miR-210-3p was found to target CDIP1 and reduce their levels upon the Ang II challenge. CDIP1 induces stress-mediated apoptosis involving the extrinsic apoptosis pathway via Bid/Bax/cleaved caspase3 activation. Administration of mimic miR-210-3p targets CDIP1 mRNA by binding to the 3' UTR region as confirmed by dual luciferase assay and also reduced Ang II-induced mitochondrial ROS accumulation as analyzed by MitoSOX staining. Moreover, the present study demonstrates the mechanism of miR-210-3p in the regulation of Ang II-induced CDIP1-associated apoptotic pathway in rADSCs.
    MeSH term(s) Adipose Tissue/cytology ; Angiotensin II/pharmacology ; Animals ; Apoptosis/drug effects ; Apoptosis/genetics ; Apoptosis/physiology ; Apoptosis Regulatory Proteins/antagonists & inhibitors ; Apoptosis Regulatory Proteins/genetics ; Apoptosis Regulatory Proteins/metabolism ; Cell Cycle Checkpoints ; Cell Hypoxia ; Cell Proliferation ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Rats ; Reactive Oxygen Species/metabolism ; Up-Regulation
    Chemical Substances Apoptosis Regulatory Proteins ; MIRN210 microRNA, rat ; MicroRNAs ; Reactive Oxygen Species ; Angiotensin II (11128-99-7)
    Language English
    Publishing date 2020-09-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2020.07.052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 116: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

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  5. Article: Inhibition of cell death-inducing p53 target 1 through miR-210-3p overexpression attenuates reactive oxygen species and apoptosis in rat adipose-derived stem cells challenged with Angiotensin II

    Lai, Chin-Hu / Barik, Parthasarathi / Hsieh, Dennis Jine-Yuan / Day, Cecilia Husan / Ho, Tsung-Jung / Chen, Ray-Jade / Kuo, Wei-Wen / Padma, V. Vijaya / Shibu, Marthandam Asokan / Huang, Chih-Yang

    Biochemical and biophysical research communications. 2020 Nov. 12, v. 532, no. 3

    2020  

    Abstract: Hypoxic preconditioning is a well-known strategy to improve the survival and therapeutic potential of stem cells against various challenges including hemodynamic and neurohormonal modulations. However, the mechanism involved in hypoxia-induced benefits ... ...

    Abstract Hypoxic preconditioning is a well-known strategy to improve the survival and therapeutic potential of stem cells against various challenges including hemodynamic and neurohormonal modulations. However, the mechanism involved in hypoxia-induced benefits on stem cells is still ambiguous. In pathological hypertension, the elevation of the neurohormonal mediator Angiotensin II (Ang II) causes the adverse effects to stem cells. In this study, we investigate the effect and mechanism of action of short term hypoxia-inducible miRNA in suppressing the effects of AngII on stem cells. According to the results obtained, Ang II affects the normal cell cycle and triggers apoptosis in rADSCs with a corresponding increase in the expression of cell death-inducing p53 target 1 (CDIP1) protein. However, the short term hypoxia-inducible miRNA-miR-210-3p was found to target CDIP1 and reduce their levels upon the Ang II challenge. CDIP1 induces stress-mediated apoptosis involving the extrinsic apoptosis pathway via Bid/Bax/cleaved caspase3 activation. Administration of mimic miR-210-3p targets CDIP1 mRNA by binding to the 3′ UTR region as confirmed by dual luciferase assay and also reduced Ang II-induced mitochondrial ROS accumulation as analyzed by MitoSOX staining. Moreover, the present study demonstrates the mechanism of miR-210-3p in the regulation of Ang II-induced CDIP1-associated apoptotic pathway in rADSCs.
    Keywords apoptosis ; cell cycle ; hemodynamics ; hypertension ; luciferase ; mechanism of action ; microRNA ; mitochondria ; rats ; reactive oxygen species ; research ; therapeutics
    Language English
    Dates of publication 2020-1112
    Size p. 347-354.
    Publishing place Elsevier Inc.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2020.07.052
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 71/706: Show issues
    Z 3.8: Show issues

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