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  1. Article ; Online: Distress in the care of people with chronic low back pain: insights from an ethnographic study.

    Dillon, Miriam / Olson, Rebecca E / Plage, Stefanie / Miciak, Maxi / Window, Peter / Stewart, Matthew / Christoffersen, Anja / Kilner, Simon / Barthel, Natalie / Setchell, Jenny

    Frontiers in sociology

    2023  Volume 8, Page(s) 1281912

    Abstract: Introduction: Distress is part of the experiences and care for people with chronic low back pain. However, distress is often pathologised and individualised; it is seen as a problem within the individual in pain and something to be downplayed, avoided, ... ...

    Abstract Introduction: Distress is part of the experiences and care for people with chronic low back pain. However, distress is often pathologised and individualised; it is seen as a problem within the individual in pain and something to be downplayed, avoided, or fixed. To that end, we situate distress as a normal everyday relational experience circulating, affecting, moving in, through, and across bodies. Challenging practices that may amplify distress, we draw on the theorisation of affect as a relational assemblage to analyse physiotherapy clinical encounters in the care of people with chronic low back pain.
    Methods: Adopting a critical reflexive ethnographic approach, we analyse data from a qualitative project involving 15 ethnographic observations of patient-physiotherapist interactions and 6 collaborative dialogues between researchers and physiotherapists. We foreground conceptualisations of distress- and what they make (im)possible-to trace embodied assemblage formations and relationality when caring for people with chronic low back pain.
    Results: Our findings indicate that conceptualisation matters to the clinical entanglement, particularly how distress is recognised and navigated. Our study highlights how distress is both a lived experience and an affective relation-that both the physiotherapist and people with chronic low back pain experience distress and can be affected by and affect each other within clinical encounters.
    Discussion: Situated at the intersection of health sociology, sociology of emotions, and physiotherapy, our study offers a worked example of applying an affective assemblage theoretical framework to understanding emotionally imbued clinical interactions. Viewing physiotherapy care through an affective assemblage lens allows for recognising that life, pain, and distress are emerging, always in flux. Such an approach recognises that clinicians and patients experience distress; they are affected by and affect each other. It demands a more humanistic approach to care and helps move towards reconnecting the inseparable in clinical practice-emotion and reason, body and mind, carer and cared for.
    Language English
    Publishing date 2023-11-16
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2297-7775
    ISSN (online) 2297-7775
    DOI 10.3389/fsoc.2023.1281912
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The proteomic landscape of genome-wide genetic perturbations.

    Messner, Christoph B / Demichev, Vadim / Muenzner, Julia / Aulakh, Simran K / Barthel, Natalie / Röhl, Annika / Herrera-Domínguez, Lucía / Egger, Anna-Sophia / Kamrad, Stephan / Hou, Jing / Tan, Guihong / Lemke, Oliver / Calvani, Enrica / Szyrwiel, Lukasz / Mülleder, Michael / Lilley, Kathryn S / Boone, Charles / Kustatscher, Georg / Ralser, Markus

    Cell

    2023  Volume 186, Issue 9, Page(s) 2018–2034.e21

    Abstract: Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces ... ...

    Abstract Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces cerevisiae, using data-independent acquisition mass spectrometry. We find that global protein expression is driven by a complex interplay of (1) general biological properties, including translation rate, protein turnover, the formation of protein complexes, growth rate, and genome architecture, followed by (2) functional properties, such as the connectivity of a protein in genetic, metabolic, and physical interaction networks. Moreover, we show that functional proteomics complements current gene annotation strategies through the assessment of proteome profile similarity, protein covariation, and reverse proteome profiling. Thus, our study reveals principles that govern protein expression and provides a genome-spanning resource for functional annotation.
    MeSH term(s) Proteomics/methods ; Proteome/metabolism ; Genomics/methods ; Genome ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism
    Chemical Substances Proteome
    Language English
    Publishing date 2023-04-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2023.03.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The proteomic landscape of genome-wide genetic perturbations

    Messner, Christoph B. / Demichev, Vadim / Muenzner, Julia / Aulakh, Simran K. / Barthel, Natalie / Röhl, Annika / Herrera-Domínguez, Lucía / Egger, Anna-Sophia / Kamrad, Stephan / Hou, Jing / Tan, Guihong / Lemke, Oliver / Calvani, Enrica / Szyrwiel, Lukasz / Mülleder, Michael / Lilley, Kathryn S. / Boone, Charles / Kustatscher, Georg / Ralser, Markus

    Cell. 2023 Apr. 19,

    2023  

    Abstract: Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces ... ...

    Abstract Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces cerevisiae, using data-independent acquisition mass spectrometry. We find that global protein expression is driven by a complex interplay of (1) general biological properties, including translation rate, protein turnover, the formation of protein complexes, growth rate, and genome architecture, followed by (2) functional properties, such as the connectivity of a protein in genetic, metabolic, and physical interaction networks. Moreover, we show that functional proteomics complements current gene annotation strategies through the assessment of proteome profile similarity, protein covariation, and reverse proteome profiling. Thus, our study reveals principles that govern protein expression and provides a genome-spanning resource for functional annotation.
    Keywords Saccharomyces cerevisiae ; genes ; genomics ; mass spectrometry ; protein synthesis ; proteome ; proteomics ; quantitative proteomics ; data-independent acquisition ; knockout ; deletion ; systems biology ; functional proteomics ; high throughput ; functional genomics ; gene annotation
    Language English
    Dates of publication 2023-0419
    Publishing place Elsevier Inc.
    Document type Article ; Online
    Note Pre-press version ; Use and reproduction
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2023.03.026
    Database NAL-Catalogue (AGRICOLA)

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