Article ; Online: Control of extensively drug-resistant Pseudomonas aeruginosa co-harboring metallo-β-lactamase enzymes with oprD gene downregulation.
Indian journal of medical microbiology
2021 Volume 40, Issue 1, Page(s) 51–56
Abstract: Purpose: to study control and treatment of infection with extensive drug-resistant carbapenem-resistant Pseudomonas aeruginosa (XDR-CRPA).: Methods: Eleven Pseudomonas aeruginosa (XDR-CRPA) strains used in this study were isolated from a clinical ... ...
Abstract | Purpose: to study control and treatment of infection with extensive drug-resistant carbapenem-resistant Pseudomonas aeruginosa (XDR-CRPA). Methods: Eleven Pseudomonas aeruginosa (XDR-CRPA) strains used in this study were isolated from a clinical sample, identified, and antibiotics susceptibility recorded in a previous study. Real-time PCR (RT-PCR) was performed to determine the expression level of the OprD gene. Besides, a checkerboard technique was performed to assess the effect of polymyxin-B (POX), colistin (COL), rifampicin (RIF), imipenem (IPM), and meropenem (MEM) during 2 and 3- dimensional antibiotic combinations. Further, the time-kill study was determined for the most potent combination against four representative strains, log Results: Molecular analysis by Real-time PCR revealed that the diminished expression level of OprD mRNA was overwhelming to various degrees. The checkerboard method demonstrated that the relevant synergism was achieved in 90.9% of strains for both carbapenem antibiotics during the triple combinations. While an additive effect was noted for all the dual regimen assays. Regarding time-kill experiments, a remarkable bactericidal effect with [99.9% killing rate] was observed toward only one strain whilst a bacteriostatic attitude was proven with ≥95% bacterial eradication against the three remaining strains. Conclusions: These findings underscore the promising implications of these combinations for treatment against XDR-Pseudomonas aeruginosa even they are resistant to carbapenems due to multiple mechanisms of action. |
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MeSH term(s) | Anti-Bacterial Agents/therapeutic use ; Carbapenems/pharmacology ; Carbapenems/therapeutic use ; Down-Regulation ; Humans ; Microbial Sensitivity Tests ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/genetics ; beta-Lactamases/genetics ; beta-Lactamases/metabolism |
Chemical Substances | Anti-Bacterial Agents ; Carbapenems ; beta-Lactamases (EC 3.5.2.6) |
Language | English |
Publishing date | 2021-11-18 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 1038798-5 |
ISSN | 1998-3646 ; 0255-0857 |
ISSN (online) | 1998-3646 |
ISSN | 0255-0857 |
DOI | 10.1016/j.ijmmb.2021.11.002 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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