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Article ; Online: Prevention, prediction, and mitigation of postoperative pancreatic fistula.

Marchegiani, G / Bassi, C

2021 Volume 108, Issue 6, Page(s) 602–604

MeSH term(s)	Humans ; Pancreatic Fistula/diagnosis ; Pancreatic Fistula/etiology ; Pancreatic Fistula/prevention & control ; Pancreatic Fistula/therapy ; Pancreaticoduodenectomy/adverse effects ; Risk Assessment
Language	English
Publishing date	2021-05-03
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2985-3
ISSN	1365-2168 ; 0263-1202 ; 0007-1323 ; 1355-7688
ISSN (online)	1365-2168
ISSN	0263-1202 ; 0007-1323 ; 1355-7688
DOI	10.1093/bjs/znab125
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Article: Simultaneous interneuron labeling reveals population-level interactions among parvalbumin, somatostatin, and pyramidal neurons in cortex.

Potter, Christian / Bassi, Constanza / Runyan, Caroline A

bioRxiv : the preprint server for biology

2024

Abstract: Cortical interneurons shape network activity in cell type-specific ways, and are also influenced by interactions with other cell types. These specific cell-type interactions are understudied, as transgenic labeling methods typically restrict labeling to ... ...

Abstract	Cortical interneurons shape network activity in cell type-specific ways, and are also influenced by interactions with other cell types. These specific cell-type interactions are understudied, as transgenic labeling methods typically restrict labeling to one neuron type at a time. Although recent methods have enabled post-hoc identification of cell types, these are not available to many labs. Here, we present a method to distinguish between two red fluorophores
Language	English
Publishing date	2024-02-02
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.01.09.523298
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Article ; Online: Surgery in Italy. Criteria to identify the hospital units and the tertiary referral centers entitled to perform it : A proposal for esophageal, hepatic, pancreatic and colo-rectal surgery.

Bassi, Claudio

Updates in surgery

2016 Volume 68, Issue 2, Page(s) 115–116

MeSH term(s)	Colorectal Neoplasms/surgery ; Digestive System Surgical Procedures/utilization ; Esophageal Neoplasms/surgery ; Hospital Units ; Humans ; Italy ; Liver Neoplasms/surgery ; Pancreatic Neoplasms/surgery ; Quality of Health Care ; Tertiary Care Centers
Language	English
Publishing date	2016-06
Publishing country	Italy
Document type	Editorial
ZDB-ID	2572692-4
ISSN	2038-3312 ; 2038-131X
ISSN (online)	2038-3312
ISSN	2038-131X
DOI	10.1007/s13304-016-0378-8
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Article ; Online: Progression from biochemical leak to pancreatic fistula after distal pancreatectomy. Don't cry over spilt amylase.

Ciprani, D / Bannone, E / Marchegiani, G / Nessi, C / Salvia, R / Bassi, C

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.

2022 Volume 22, Issue 6, Page(s) 817–822

Abstract: Background: Postoperative pancreatic fistula (POPF) is a frequent complication after distal pancreatectomy (DP), but its upgrading from biochemical leak (BL) still represents an unexplored phenomenon. This study aims at identifying risk factors of the ... ...

Abstract	Background: Postoperative pancreatic fistula (POPF) is a frequent complication after distal pancreatectomy (DP), but its upgrading from biochemical leak (BL) still represents an unexplored phenomenon. This study aims at identifying risk factors of the clinical evolution from BL to grade-B POPF after DP. Methods: Patients who underwent DP between 2015 and 2019 and who developed either BL (n = 89,56%) or BL upgraded to late B fistula (LB) after postoperative day 5 (n = 71,44%) were included. Preoperative, surgical, postoperative predictors were compared between the two groups. Results: Patients with LB were significantly older (61 vs 56 years, P < 0.025) and received neoadjuvant chemotherapy more frequently (22.5% vs 8.5%,P = 0.017). Extended lymphadenectomy (52.8% vs 31.0%,P = 0.006), longer operative times (OT) (307 vs 250 min,P = 0.002), greater estimated blood loss (250 vs 150 ml, P = 0.021), and the appearance of purulent fluid in surgical drains (58.4% vs 21.1%; P < 0.001) were more frequently observed in LB group. Only purulent fluid in surgical drains and longer OT were confirmed as independent predictors of BL clinical progression. Conclusions: Purulent fluid from surgical drains should be suspicious of BL upgrading. Frail patients undergoing longer interventions may represent key targets of mitigation strategies to minimize the magnitude of an incipient fistula and its increase in morbidity.
MeSH term(s)	Amylases ; Drainage/adverse effects ; Humans ; Pancreatectomy/adverse effects ; Pancreatic Fistula/complications ; Pancreatic Fistula/therapy ; Postoperative Complications/etiology ; Retrospective Studies ; Risk Factors
Chemical Substances	Amylases (EC 3.2.1.-)
Language	English
Publishing date	2022-06-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2056680-3
ISSN	1424-3911 ; 1424-3903
ISSN (online)	1424-3911
ISSN	1424-3903
DOI	10.1016/j.pan.2022.06.257
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Article ; Online: Sex disparities in clinical features and burden of narcolepsy type 1.

Ingravallo, Francesca / Bassi, Chiara / Zenesini, Corrado / Vignatelli, Luca / Pagotto, Uberto / Pizza, Fabio / Plazzi, Giuseppe

Journal of sleep research

2024 , Page(s) e14157

Abstract: To investigate potential sex-related differences in patients with narcolepsy type 1, we carried out an analysis of baseline data from 93 women and 89 men with narcolepsy type 1 who participated in the TElemedicine for NARcolepsy (TENAR) trial. The ... ...

Abstract	To investigate potential sex-related differences in patients with narcolepsy type 1, we carried out an analysis of baseline data from 93 women and 89 men with narcolepsy type 1 who participated in the TElemedicine for NARcolepsy (TENAR) trial. The following data were considered: sociodemographics; diagnostic (disease history, polysomnography, orexin, human leukocyte antigen) and clinical features, including sleepiness (Epworth Sleepiness Scale), cataplexy and other narcolepsy symptoms; disease severity (Narcolepsy Severity Scale); pharmacological treatment; depressive symptoms (Beck Depression Inventory); and self-reported relevance of eight narcolepsy-related issues. We found that, compared with men, significantly more women reported automatic behaviours (55.4% versus 40%) and had higher Epworth Sleepiness Scale (median 10 versus 9) and Beck Depression Inventory scores (median 10.5 versus 5), and there was a trend for a higher Narcolepsy Severity Scale total score in women (median 19 versus 18, p = 0.057). More women than men were officially recognized as having a disability (38% versus 22.5%) and considered 5/8 narcolepsy-related issues investigated as a relevant problem. More severe sleepiness and a greater narcolepsy-related burden in women could mirror sex differences present in the general population, or may be related to suboptimal management of narcolepsy type 1 or to more severe depressive symptoms in women. Future studies and guidelines should address these aspects.
Language	English
Publishing date	2024-02-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1122722-9
ISSN	1365-2869 ; 0962-1105
ISSN (online)	1365-2869
ISSN	0962-1105
DOI	10.1111/jsr.14157
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Article: Declining greenhouse gas emissions in the US diet (2003–2018): Drivers and demographic trends

Bassi, Clare / Maysels, Rachael / Anex, Rob

Journal of cleaner production. 2022 Mar. 19,

2022

Abstract: The food system is a major driver of climate change, and many have noted that a shift in consumption patterns is necessary to achieve greenhouse gas (GHG) emission reduction targets that can limit global mean temperature rise ≤2 °C. Beef is the largest ... ...

Abstract	The food system is a major driver of climate change, and many have noted that a shift in consumption patterns is necessary to achieve greenhouse gas (GHG) emission reduction targets that can limit global mean temperature rise ≤2 °C. Beef is the largest GHG emitting commodity in the United States, and in recent years national consumption has been declining. Little is understood about how this change in consumption and other dietary trends have influenced the overall GHGs associated with the US diet. The objective of this study is to estimate the GHGs of changing dietary patterns from individual self-selected diets in the United States from 2003-2018 and evaluate trends and potential disparities among demographic subgroups. Life cycle emissions factors (representing food production impacts) for food commodities from dataFIELD were used to estimate GHGs associated with food items described by US adults (>20 years, n = 39,750) in the National Health and Nutrition Examination Survey (NHANES). From 2003 to 2018, the mean GHG emissions associated with the US diet fell by more than 35%, from 4.02kg CO₂e per day per capita, to 2.45kg CO₂e per day per capita, despite average caloric intake remaining relatively stable over the same period. Average beef consumption declined 40% per capita over the study period, which contributed to more than 50% of the observed GHG savings in the diet over the study period. All demographic variables included in this analysis (age, gender, race/ethnicity, and ratio of family income to the federal poverty level) exhibited a reduction in GHG emissions associated with their diets. However, GHGs and overall rate of change differed across demographic subgroups. Black women had the lowest GHG emissions associated with their diet, 1.92kg CO₂e per capita per day. Men aged 20–34 had the largest rate of reduction in GHGs associated with diet changes, with an average annual decline of 210g CO₂e per day per capita over the study period. Despite GHGs associated with the US diet falling over the last 15 years, the US diet is still exceeding established GHG limits to meet global targets, such as the Paris Agreement. Additional research is needed to better understand motivations and drivers that have reduced emissions in the diet over this period, particularly in demographic subgroups that showed both low impact and a rapid decline in emissions.
Keywords	National Health and Nutrition Examination Survey ; United Nations Framework Convention on Climate Change ; air temperature ; beef ; beef consumption ; climate change ; energy intake ; food production ; gender ; greenhouse gases ; household income ; poverty
Language	English
Dates of publication	2022-0319
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ISSN	0959-6526
DOI	10.1016/j.jclepro.2022.131465
Database	NAL-Catalogue (AGRICOLA)

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Article: Duodénopancréatectomie céphalique pour cancer.

Bassi, C

Journal de chirurgie

2008 Volume 145, Issue 1, Page(s) 6–8

Title translation	Pancreaticoduodenectomy for cancer.
MeSH term(s)	Humans ; Neoadjuvant Therapy/methods ; Pancreatic Neoplasms/surgery ; Pancreatic Neoplasms/therapy ; Pancreaticoduodenectomy/methods ; Postoperative Care ; Preoperative Care ; Prognosis ; Treatment Outcome
Language	French
Publishing date	2008-04-22
Publishing country	France
Document type	Comment ; Editorial
ZDB-ID	218138-1
ISSN	1773-0422 ; 0021-7697
ISSN (online)	1773-0422
ISSN	0021-7697
DOI	10.1016/s0021-7697(08)70281-3
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Article ; Online: Decision points in pancreatoduodenectomy: Insights from the contemporary experts on prevention, mitigation, and management of postoperative pancreatic fistula.

Casciani, Fabio / Bassi, Claudio / Vollmer, Charles M

Surgery

2021 Volume 170, Issue 3, Page(s) 889–909

Abstract: Background: Despite abundant, high-level scientific evidence, there is no consensus regarding the prevention, mitigation, and management of clinically relevant pancreatic fistula after pancreatoduodenectomy. The aim of the present investigation is three- ...

Abstract	Background: Despite abundant, high-level scientific evidence, there is no consensus regarding the prevention, mitigation, and management of clinically relevant pancreatic fistula after pancreatoduodenectomy. The aim of the present investigation is three-fold: (1) to analyze the multiple decision-making points for pancreatico-enteric anastomotic creation and fistula mitigation and management after pancreatoduodenectomy, (2) to reveal the practice of contemporary experts, and (3) to indicate avenues for future research to reduce the burden of clinically relevant pancreatic fistula. Methods: A 109-item questionnaire was sent to a panel of international pancreatic surgery experts, recognized for their clinical and scientific authority. Their practice habits and thought processes regarding clinically relevant pancreatic fistula risk assessment, anastomotic construction, application of technical adjuncts, and mitigation strategies, as well as postoperative management, was explored. Sixteen clinical vignettes were presented to reveal their certain approaches to unique situations-both common and uncommon. Results: Sixty experts, with a cumulative 48,860 pancreatoduodenectomies, completed the questionnaire. Their median pancreatectomy/pancreatoduodenectomy case volume was 1,200 and 705 procedures, respectively, with a median career duration of 22 years and 200 indexed publications. Although pancreatico-jejunostomy reconstruction with transperitoneal drainage is the standard operative approach for most authorities, uncertainty emerges regarding the employment of objective risk stratification and adaptation of practice to risk. Concrete suggestions are offered to inform decision-making in intimidating circumstances. Early drain removal is frequently embraced, while a step-up approach is unanimously invoked to treat severe clinically relevant pancreatic fistula. Conclusion: A comprehensive conceptual framework of 4 sequential phases of decision-making is proposed-risk assessment, anastomotic technique, mitigation strategy employment, and postoperative management. Basic science studies and outcome analyses are proposed for improvement.
MeSH term(s)	Adult ; Anastomosis, Surgical/adverse effects ; Anastomosis, Surgical/methods ; Clinical Decision-Making/methods ; Female ; Humans ; Male ; Middle Aged ; Pancreas/surgery ; Pancreatic Fistula/etiology ; Pancreatic Fistula/prevention & control ; Pancreatic Fistula/therapy ; Pancreaticoduodenectomy/adverse effects ; Pancreaticoduodenectomy/methods ; Risk Assessment ; Surgeons/statistics & numerical data ; Surveys and Questionnaires
Language	English
Publishing date	2021-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	202467-6
ISSN	1532-7361 ; 0039-6060
ISSN (online)	1532-7361
ISSN	0039-6060
DOI	10.1016/j.surg.2021.02.064
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Article: Novel Virus Identification through Metagenomics: A Systematic Review.

Bassi, Cristian / Guerriero, Paola / Pierantoni, Marina / Callegari, Elisa / Sabbioni, Silvia

Life (Basel, Switzerland)

2022 Volume 12, Issue 12

Abstract: Metagenomic Next Generation Sequencing (mNGS) allows the evaluation of complex microbial communities, avoiding isolation and cultivation of each microbial species, and does not require prior knowledge of the microbial sequences present in the sample. ... ...

Abstract	Metagenomic Next Generation Sequencing (mNGS) allows the evaluation of complex microbial communities, avoiding isolation and cultivation of each microbial species, and does not require prior knowledge of the microbial sequences present in the sample. Applications of mNGS include virome characterization, new virus discovery and full-length viral genome reconstruction, either from virus preparations enriched in culture or directly from clinical and environmental specimens. Here, we systematically reviewed studies that describe novel virus identification through mNGS from samples of different origin (plant, animal and environment). Without imposing time limits to the search, 379 publications were identified that met the search parameters. Sample types, geographical origin, enrichment and nucleic acid extraction methods, sequencing platforms, bioinformatic analytical steps and identified viral families were described. The review highlights mNGS as a feasible method for novel virus discovery from samples of different origins, describes which kind of heterogeneous experimental and analytical protocols are currently used and provides useful information such as the different commercial kits used for the purification of nucleic acids and bioinformatics analytical pipelines.
Language	English
Publishing date	2022-12-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life12122048
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Article ; Online: Adjuvant Therapy After Upfront Resection of Resectable Pancreatic Cancer: Patterns of Omission and Use-A Prospective Real-Life Study.

Paiella, Salvatore / Malleo, Giuseppe / Lionetto, Gabriella / Cattelani, Alice / Casciani, Fabio / Secchettin, Erica / De Pastena, Matteo / Bassi, Claudio / Salvia, Roberto

Annals of surgical oncology

2024 Volume 31, Issue 5, Page(s) 2892–2901

Abstract: Background: Little is known about adjuvant therapy (AT) omission and use outside of randomized trials. We aimed to assess the patterns of AT omission and use in a cohort of upfront resected pancreatic cancer patients in a real-life scenario.: Methods!# ...

Abstract	Background: Little is known about adjuvant therapy (AT) omission and use outside of randomized trials. We aimed to assess the patterns of AT omission and use in a cohort of upfront resected pancreatic cancer patients in a real-life scenario. Methods: From January 2019 to July 2022, 317 patients with resected pancreatic cancer and operated upfront were prospectively enrolled in this prospective observational trial according to the previously calculated sample size. The association between perioperative variables and the risk of AT omission and AT delay was analyzed using multivariable logistic regression. Results: Eighty patients (25.2%) did not receive AT. The main reasons for AT omission were postoperative complications (38.8%), oncologist's choice (21.2%), baseline comorbidities (20%), patient's choice (10%), and early recurrence (10%). At the multivariable analysis, the odds of not receiving AT increased significantly for older patients (odds ratio [OR] 1.1, p < 0.001), those having an American Society of Anesthesiologists score ≥II (OR 2.03, p = 0.015), or developing postoperative pancreatic fistula (OR 2.5, p = 0.019). The likelihood of not receiving FOLFIRINOX as AT increased for older patients (OR 1.1, p < 0.001), in the presence of early-stage disease (stage I-IIa vs. IIb-III, OR 2.82, p =0.031; N0 vs. N+, OR 3, p = 0.03), and for patients who experienced postoperative major complications (OR 4.7, p = 0.009). A twofold increased likelihood of delay in AT was found in patients experiencing postoperative complications (OR 3.86, p = 0.011). Conclusions: AT is not delivered in about one-quarter of upfront resected pancreatic cancer patients. Age, comorbidities, and postoperative complications are the main drivers of AT omission and mFOLFIRINOX non-use. Clinicaltrials registration: NCT03788382.
MeSH term(s)	Humans ; Pancreatic Neoplasms/therapy ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Prospective Studies ; Neoadjuvant Therapy ; Postoperative Complications ; Chemotherapy, Adjuvant
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Observational Study ; Journal Article
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-024-14951-4
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