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  1. Article ; Online: In Vitro Culture of Embryos from LOPU-Derived Goat Oocytes.

    Souza-Fabjan, Joanna Maria G / Batista, Ribrio Ivan T P / Freitas, Vicente José F / Mermillod, Pascal

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 2006, Page(s) 141–153

    Abstract: A high oocyte quality is the prerequisite for in vitro embryo production. Goat cumulus-oocyte complexes (COC) are mainly collected from slaughterhouse ovaries or by laparoscopic ovum pickup (LOPU) from live animals. Several features can influence the ... ...

    Abstract A high oocyte quality is the prerequisite for in vitro embryo production. Goat cumulus-oocyte complexes (COC) are mainly collected from slaughterhouse ovaries or by laparoscopic ovum pickup (LOPU) from live animals. Several features can influence the availability of good quality oocytes recovered by the LOPU technique. Interestingly, slaughterhouse and LOPU oocytes have different in vitro maturation kinetics and requirements, and thus, the IVP system must be adapted regarding the oocyte origin. Overall, the use of undefined media in the different steps makes interpretation of results more difficult, hampers their reproducibility, and introduces a sanitary risk. Thus, there is an effort worldwide to use simpler conditions for goat IVP. Although the success of IVP rates is relatively high, in vitro embryos differ from in vivo-derived ones in many aspects, resulting in lower viability. Therefore, strategies to improve in vitro embryo quality are crucial, such as the use of oviductal epithelium cells for coculture. Here we describe the main steps and culture media which can be utilized to produce embryos in vitro from LOPU or slaughterhouse oocytes in goats.
    MeSH term(s) Animals ; Blastocyst/cytology ; Blastocyst/metabolism ; Embryo Culture Techniques/methods ; Female ; Fertilization in Vitro/methods ; Goats ; In Vitro Oocyte Maturation Techniques/methods ; Laparoscopy ; Oocytes/cytology ; Oocytes/metabolism
    Language English
    Publishing date 2019-06-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9566-0_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique.

    Brair, Viviane L / Maia, Ana Lucia R S / Correia, Lucas Francisco L / Barbosa, Nathalia O / Santos, Juliana D R / Brandão, Felipe Z / Fonseca, Jeferson F / Batista, Ribrio Ivan T P / Souza-Fabjan, Joanna M G

    Cryobiology

    2020  Volume 95, Page(s) 110–115

    Abstract: Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy ... ...

    Abstract Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopreservation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n = 32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n = 15), slow freezing (SF; n = 42) or vitrification (VT; n = 43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 °C and 5% CO
    MeSH term(s) Animals ; Blastocyst ; Cryopreservation/methods ; Embryo Transfer ; Female ; Freezing ; Gene Expression ; Pregnancy ; Sheep ; Vitrification
    Language English
    Publishing date 2020-06-15
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80098-3
    ISSN 1090-2392 ; 0011-2240
    ISSN (online) 1090-2392
    ISSN 0011-2240
    DOI 10.1016/j.cryobiol.2020.05.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 528: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  3. Article: Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique

    Brair, Viviane L / Maia, Ana Lucia R.S / Correia, Lucas Francisco L / Barbosa, Nathalia O / Santos, Juliana D.R / Brandão, Felipe Z / Fonseca, Jeferson F / Batista, Ribrio Ivan T.P / Souza-Fabjan, Joanna M.G

    Cryobiology. 2020 Aug., v. 95

    2020  

    Abstract: Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy ... ...

    Abstract Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopreservation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n = 32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n = 15), slow freezing (SF; n = 42) or vitrification (VT; n = 43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 °C and 5% CO₂ (SF: n = 27 and VT: n = 28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P > 0.05). Only CDX2 was affected (up-regulated, P < 0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P < 0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF.
    Keywords apoptosis ; blastocyst ; carbon dioxide ; cell proliferation ; cryobiology ; cryopreservation ; endometrium ; gene expression ; mitochondria ; pregnancy rate ; superovulation ; survival rate ; vitrification
    Language English
    Dates of publication 2020-08
    Size p. 110-115.
    Publishing place Elsevier Inc.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 80098-3
    ISSN 1090-2392 ; 0011-2240
    ISSN (online) 1090-2392
    ISSN 0011-2240
    DOI 10.1016/j.cryobiol.2020.05.009
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 528: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes.

    Bragança, Gláucia M / Batista, Ribrio Ivan T P / Souza-Fabjan, Joanna Maria G / Alfradique, Vivian A P / Arashiro, Eduardo K N / Cosentino, Isabel O / Pinto, Pedro Henrique N / Camargo, Luiz Sérgio A / da Fonseca, Jeferson F / Brandão, Felipe Z

    Reproduction, fertility, and development

    2018  Volume 30, Issue 9, Page(s) 1234–1244

    Abstract: The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a ... ...

    Abstract The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ERα, oestrogen receptor α; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ERα in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes.
    MeSH term(s) Animals ; Cumulus Cells/drug effects ; Cumulus Cells/metabolism ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Female ; Follicle Stimulating Hormone/administration & dosage ; Gene Expression/drug effects ; Oocytes/drug effects ; Oocytes/metabolism ; Ovulation Induction/methods ; Sheep
    Chemical Substances Follicle Stimulating Hormone (9002-68-0)
    Language English
    Publishing date 2018-03-26
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 1019913-5
    ISSN 1448-5990 ; 1031-3613
    ISSN (online) 1448-5990
    ISSN 1031-3613
    DOI 10.1071/RD17337
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2756: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  5. Article ; Online: Dynamics of recombinant hG-CSF in transgenic goat: preliminary study in the founder during hormonally induced lactation.

    Moura, Raylene R / Albuquerque, Erica S / Melo, Carlos Henrique S / Alcântara-Neto, Agostinho S / Batista, Ribrio Ivan T P / Nunes-Pinheiro, Diana Célia S / Pereira, Alexsandra F / Teixeira, Darcio Ítalo A / Melo, Luciana M / Serova, Irina A / Andreeva, Lyudmila E / Serov, Oleg L / Freitas, Vicente José F

    Animal biotechnology

    2013  Volume 24, Issue 1, Page(s) 10–14

    Abstract: This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 ... ...

    Abstract This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 µg hG-CSF per mL of milk. Two peaks of serum hG-CSF (3,470 and 7,390 pg/mL) were detected in the first half of the lactation. Outside of the lactation, hG-CSF was absent from serum, indicating no ectopic expression. During the treatment to induce lactation, transgenic female presented increased neutrophil and lymphocyte blood counts when compared to nontransgenic female. Despite transient neutrophilia, serum biochemistry profiles indicated normal liver and renal functions. Thus, transgenic goat expressed hG-CSF in quantities sufficient for a commercial bioreactor and remained clinically healthy.
    MeSH term(s) Animals ; Animals, Genetically Modified/genetics ; Animals, Genetically Modified/metabolism ; Female ; Goats/genetics ; Goats/metabolism ; Granulocyte Colony-Stimulating Factor/blood ; Granulocyte Colony-Stimulating Factor/genetics ; Granulocyte Colony-Stimulating Factor/metabolism ; Hormones/pharmacology ; Humans ; Lactation/drug effects ; Lactation/genetics ; Lactation/metabolism ; Leukocyte Count ; Milk/chemistry ; Milk/metabolism ; Recombinant Proteins/blood ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
    Chemical Substances Hormones ; Recombinant Proteins ; Granulocyte Colony-Stimulating Factor (143011-72-7)
    Language English
    Publishing date 2013-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2043243-4
    ISSN 1532-2378 ; 1049-5398
    ISSN (online) 1532-2378
    ISSN 1049-5398
    DOI 10.1080/10495398.2012.729000
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Dynamics of Recombinant hG-CSF in Transgenic Goat: Preliminary Study in the Founder during Hormonally Induced Lactation

    Moura, Raylene R / Albuquerque, Erica S / Melo, Carlos Henrique S / Alcântara-Neto, Agostinho S / Batista, Ribrio Ivan T. P / Nunes-Pinheiro, Diana Célia S / Pereira, Alexsandra F / Teixeira, [Dacute]arcio Ítalo A / Melo, Luciana M / Serova, Irina A / Andreeva, Lyudmila E / Serov, Oleg L / Freitas, Vicente José F

    Animal biotechnology. 2013 Jan. 1, v. 24, no. 1

    2013  

    Abstract: This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 ... ...

    Abstract This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 µg hG-CSF per mL of milk. Two peaks of serum hG-CSF (3,470 and 7,390 pg/mL) were detected in the first half of the lactation. Outside of the lactation, hG-CSF was absent from serum, indicating no ectopic expression. During the treatment to induce lactation, transgenic female presented increased neutrophil and lymphocyte blood counts when compared to nontransgenic female. Despite transient neutrophilia, serum biochemistry profiles indicated normal liver and renal functions. Thus, transgenic goat expressed hG-CSF in quantities sufficient for a commercial bioreactor and remained clinically healthy.
    Keywords blood ; goats ; granulocyte colony-stimulating factor ; humans ; lactation ; liver ; milk
    Language English
    Dates of publication 2013-0101
    Size p. 10-14.
    Publishing place Taylor & Francis Group
    Document type Article
    ZDB-ID 2043243-4
    ISSN 1532-2378 ; 1049-5398
    ISSN (online) 1532-2378
    ISSN 1049-5398
    DOI 10.1080/10495398.2012.729000
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Dynamics of Recombinant hG-CSF in Transgenic Goat: Preliminary Study in the Founder during Hormonally Induced Lactation

    Moura, Raylene R. / Albuquerque, Erica S. / Melo, Carlos Henrique S. / Alcântara-Neto, Agostinho S. / Batista, Ribrio Ivan T. P. / Nunes-Pinheiro, Diana Célia S. / Pereira, Alexsandra F. / Teixeira, [Dacute]arcio Ítalo A. / Melo, Luciana M. / Serova, Irina A. / Andreeva, Lyudmila E. / Serov, Oleg L. / Freitas, Vicente José F.

    Animal biotechnology

    Volume v. 24,, Issue no. 1

    Abstract: This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 ... ...

    Abstract This study aimed to characterize the dynamic of human granulocyte colony-stimulating factor (hG-CSF) during artificial lactation in a transgenic founder goat and to assess its potential ectopic expression and health. The female secreted 93.9 to 1,474.6 µg hG-CSF per mL of milk. Two peaks of serum hG-CSF (3,470 and 7,390 pg/mL) were detected in the first half of the lactation. Outside of the lactation, hG-CSF was absent from serum, indicating no ectopic expression. During the treatment to induce lactation, transgenic female presented increased neutrophil and lymphocyte blood counts when compared to nontransgenic female. Despite transient neutrophilia, serum biochemistry profiles indicated normal liver and renal functions. Thus, transgenic goat expressed hG-CSF in quantities sufficient for a commercial bioreactor and remained clinically healthy.
    Keywords lactation ; granulocyte colony-stimulating factor ; goats ; milk ; liver ; humans ; blood
    Language English
    Document type Article
    ISSN 1532-2378
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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