Article ; Online: Predictive factors of COVID-19 in patients with negative RT-qPCR.
Semergen
2020 Volume 46 Suppl 1, Page(s) 6–11
Abstract: Objective: To evaluate the factors associated with false negatives in RT-qPCR in patients with mild-moderate symptoms of COVID-19.: Materials and methods: This was a cross-sectional study that used a random sample of non-hospitalized patients from ... ...
| Abstract | Objective: To evaluate the factors associated with false negatives in RT-qPCR in patients with mild-moderate symptoms of COVID-19. Materials and methods: This was a cross-sectional study that used a random sample of non-hospitalized patients from the primary care management division of the Healthcare Area of Leon (58 RT-qPCR-positive cases and 52 RT-qPCR-negative cases). Information regarding symptoms was collected and all patients were simultaneously tested using two rapid diagnostic tests - RDTs (Combined - cRDT and Differentiated - dRDT). The association between symptoms and SARS-CoV-2 infection was evaluated by non-conditional logistic regression, with estimation of Odds Ratio. Results: A total of 110 subjects were studied, 52% of whom were women (mean age: 48.2±11.0 years). There were 42.3% of negative RT-qPCRs that were positive in some RDTs. Fever over 38°C (present in 35.5% of cases) and anosmia (present in 41.8%) were the symptoms most associated with SARS-CoV-2 infection, a relationship that remained statistically significant in patients with negative RT-qPCR and some positive RDT (aOR=6.64; 95%CI=1.33-33.13 and aOR=19.38; 95% CI=3.69-101.89, respectively). Conclusions: RT-qPCR is the technique of choice in the diagnosis of SARS-CoV-2 infection, but it is not exempt from false negatives. Our results show that patients who present mild or moderate symptoms with negative RT-qPCR, but with fever and/or anosmia, should be considered as suspicious cases and should be evaluated with other diagnostic methods. |
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| MeSH term(s) | Adult ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Cross-Sectional Studies ; False Negative Reactions ; Female ; Humans ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnosis ; Predictive Value of Tests |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-06-23 |
| Publishing country | Spain |
| Document type | Journal Article |
| ISSN | 1578-8865 |
| ISSN (online) | 1578-8865 |
| DOI | 10.1016/j.semerg.2020.06.010 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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