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  1. Article ; Online: Positive effects of Phycocyanobilin on gene expression in glutamate-induced excitotoxicity in SH-SY5Y cells and animal models of multiple sclerosis and cerebral ischemia

    Daniel Palenzuela Gardón / Majel Cervantes-Llanos / Beatriz Piniella Matamoros / Hanlet Camacho Rodríguez / Chan-yuan Tan / Javier Marín –Prida / Viviana Falcón-Cama / Nancy Pavón-Fuentes / Jessica Gómez Lemus / Laura de la Caridad Bakos Ruiz / Tamara Díaz Argudin / Gillian Martínez Donato / Yasser Perera / Ke Yang / Giselle Pentón-Rol

    Heliyon, Vol 8, Iss 6, Pp e09769- (2022)

    2022  

    Abstract: Background: Oxidative stress has a predominant role in the pathogenesis of neurodegenerative diseases and therefore the modulation of genes and the identification of biological pathways associated with antioxidant therapies, have an impact on its ... ...

    Abstract Background: Oxidative stress has a predominant role in the pathogenesis of neurodegenerative diseases and therefore the modulation of genes and the identification of biological pathways associated with antioxidant therapies, have an impact on its treatment. Objective: The objective of this study was the comparison of 2 methods for the analysis of real-time PCR (qPCR) data, through the use of the evaluation of genes that mediate the effect of Phycocyanobilin (PCB) and its validation in animal models. Methods: We evaluated the effect of PCB:” in vitro” on gene modulation through qPCR analyzed by parametric ANOVA and multivariate principal component analysis (PCA) in a model of glutamate-induced excitotoxicity in the SH-SY5Y cell line and” in vivo”; in animal models of multiple sclerosis (MS) and cerebral ischemia (CI). Results: The results showed that PCA is a robust and powerful method that allows the assessment of gene expression profiles. We detected the significant down-regulation of the CYBB (NOX2), and HMOX1 by the action of PCB in SH-5YSH cell line insulted with Glutamate. The decrease in pro-inflammatory cytokines and markers related to apoptosis and innate immune response, mediated the effect of PCB in the animal models of MS and CI, respectively. Conclusion: We concluded that the mechanisms by which PCB protected cells included the reduction of oxidative stress damage, which could contribute to its clinical efficacy for the treatment of neurodegenerative diseases.
    Keywords Phycocyanobilin ; qPCR ; PCA ; Glutamate ; Oxidative stress ; Multiple sclerosis ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Subject code 610
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Beneficial effects of oral administration of C-Phycocyanin and Phycocyanobilin in rodent models of experimental autoimmune encephalomyelitis

    Cervantes-Llanos, Majel / Beatriz Piniella-Matamoros / Carmen Valenzuela-Silva / Eduardo Pentón-Arias / Giselle Pentón-Rol / Hanlet Camacho-Rodríguez / Ivette Raíces-Cruz / Javier Marín-Prida / Julio Raúl Fernández-Massó / Mauro Martins Teixeira / Nancy Pavón-Fuentes / Nielsen Lagumersindez-Denis / Viviana Falcon-Cama

    Life sciences. 2018 Feb. 01, v. 194

    2018  

    Abstract: The only three oral treatments currently available for multiple sclerosis (MS) target the relapsing forms of the disease and concerns regarding efficacy, safety and tolerability limit their use. Identifying novel oral disease-modifying therapies for MS, ... ...

    Abstract The only three oral treatments currently available for multiple sclerosis (MS) target the relapsing forms of the disease and concerns regarding efficacy, safety and tolerability limit their use. Identifying novel oral disease-modifying therapies for MS, targeting both its inflammatory and neurodegenerative components is still a major goal.The scope of this study was to provide evidence that the oral administration of C-Phycocyanin (C-PC), the main biliprotein of the Spirulina platensis cyanobacteria and its tetrapyrrolic prosthetic group, Phycocyanobilin (PCB), exert ameliorating actions on rodent models of experimental autoimmune encephalomyelitis (EAE).EAE was induced in Lewis rats using the spinal cord encephalitogen from Sprague Dawley rats and in C57BL6 mice with MOG35–55 peptide. Clinical signs, motor function, oxidative stress markers, cytokine levels by ELISA and transmission electron microscopy analysis were assessed.Either prophylactic or early therapeutic administration of C-PC to Lewis rats with EAE, significantly improved clinical signs and restored the motor function of the animals. Furthermore, C-PC positively modulated oxidative stress markers measured in brain homogenate and serum and protected the integrity of cerebral myelin sheaths as shown by transmission electron microscopy analysis. In C57BL/6 mice with EAE, PCB orally improved clinical status of the animals and reduced the expression levels of brain IL-6 and IFN-γ proinflammatory cytokines.These results, for the first time, support the fact that both C-PC and PCB administered orally could potentially improve neuroinflammation, protect from demyelination and axonal loss, which may be translated into an improved quality of life for MS patients.
    Keywords animal models ; Arthrospira platensis ; blood serum ; brain ; encephalitis ; enzyme-linked immunosorbent assay ; interferon-gamma ; interleukin-6 ; mice ; oral administration ; oxidative stress ; patients ; quality of life ; rats ; sclerosis ; signs and symptoms (animals and humans) ; spinal cord ; therapeutics ; transmission electron microscopy
    Language English
    Dates of publication 2018-0201
    Size p. 130-138.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 3378-9
    ISSN 1879-0631 ; 0024-3205
    ISSN (online) 1879-0631
    ISSN 0024-3205
    DOI 10.1016/j.lfs.2017.12.032
    Database NAL-Catalogue (AGRICOLA)

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