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  1. Article: Comparison of alternative arterial anastomosis site during liver transplantation when the recipient's hepatic artery is unusable.

    Beaurepaire, Jean Marie / Orlando, Francesco / Levi Sandri, Giovanni Battista / Jezequel, Caroline / Bardou-Jacquet, Edouard / Camus, Christophe / Lakehal, Mohamed / Desfourneaux, Veronique / Merdrignac, Aude / Gaignard, Elodie / Thobie, Alexandre / Bergeat, Damien / Meunier, Bernard / Rayar, Michel

    Hepatobiliary surgery and nutrition

    2022  Volume 11, Issue 1, Page(s) 1–12

    Abstract: Background: Few studies have analyzed outcomes of liver transplantation (LT) when the recipient hepatic artery (HA) was not usable.: Methods: We retrospectively evaluated the outcomes of LT performed using the different alternative sites to HA.: ... ...

    Abstract Background: Few studies have analyzed outcomes of liver transplantation (LT) when the recipient hepatic artery (HA) was not usable.
    Methods: We retrospectively evaluated the outcomes of LT performed using the different alternative sites to HA.
    Results: Between 2002 and 2017, 1,677 LT were performed in our institution among which 141 (8.4%) with unusable recipient HA were analyzed. Four groups were defined according to the site of anastomosis: the splenic artery (SA group, n=26), coeliac trunk (CT group, n=12), aorta using or not the donor's vessel (Ao group, n=91) and aorta using a vascular prosthesis (Ao-P group, n=12) as conduit. The median number of intraoperative red blood cell transfusions was significantly increased in the Ao and Ao-P groups (5, 5, 8.5 and 16 for SA, CT, Ao and Ao-P group respectively, P=0.002), as well as fresh frozen plasma (4.5, 2.5, 10, 17 for the SA, CT, Ao and Ao-P groups respectively, P=0.001). Hospitalization duration was also significantly increased in the Ao and Ao-P groups (15, 16, 24, 26.5 days for the SA, CT, Ao and Ao-P groups respectively, P<0.001). The occurrence of early allograft dysfunction (EAD) (P=0.07) or arterial complications (P=0.26) was not statistically different. Level of factor V, INR, bilirubin and creatinine during the 7
    Conclusions: In case of unusable HA, intraoperative and postoperative outcomes are improved when using the SA or CT compared to aorta.
    Language English
    Publishing date 2022-02-24
    Publishing country China (Republic : 1949- )
    Document type Journal Article
    ZDB-ID 2812398-0
    ISSN 2304-389X ; 2304-3881
    ISSN (online) 2304-389X
    ISSN 2304-3881
    DOI 10.21037/hbsn-20-10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Tacrolimus: Does direct glucuronidation matter? An analytical and pharmacological perspective.

    Tron, Camille / Petitcollin, Antoine / Verdier, Marie-Clémence / Rayar, Michel / Beaurepaire, Jean-Marie / Boudjema, Karim / Bellissant, Eric / Lemaitre, Florian

    Pharmacological research

    2017  Volume 124, Page(s) 164–166

    MeSH term(s) Drug Monitoring/methods ; Glucuronides/metabolism ; Glucuronosyltransferase/metabolism ; Humans ; Immunosuppressive Agents/pharmacokinetics ; Immunosuppressive Agents/therapeutic use ; Tacrolimus/pharmacokinetics ; Tacrolimus/therapeutic use
    Chemical Substances Glucuronides ; Immunosuppressive Agents ; Glucuronosyltransferase (EC 2.4.1.17) ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2017-10
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 1003347-6
    ISSN 1096-1186 ; 0031-6989 ; 1043-6618
    ISSN (online) 1096-1186
    ISSN 0031-6989 ; 1043-6618
    DOI 10.1016/j.phrs.2017.03.027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Hypothermic Oxygenated Perfusion Improves Extended Criteria Donor Liver Graft Function and Reduces Duration of Hospitalization Without Extra Cost: The PERPHO Study.

    Rayar, Michel / Beaurepaire, Jean-Marie / Bajeux, Emma / Hamonic, Stéphanie / Renard, Thomas / Locher, Clara / Desfourneaux, Véronique / Merdrignac, Aude / Bergeat, Damien / Lakehal, Mohamed / Sulpice, Laurent / Houssel-Debry, Pauline / Jezequel, Caroline / Camus, Christophe / Bardou-Jacquet, Edouard / Meunier, Bernard

    Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

    2020  Volume 27, Issue 3, Page(s) 349–362

    Abstract: Few studies have evaluated the efficacy or the cost of hypothermic oxygenated perfusion (HOPE) in the conservation of extended criteria donor (ECD) grafts from donation after brain death (DBD) donors during liver transplantation (LT). We performed a ... ...

    Abstract Few studies have evaluated the efficacy or the cost of hypothermic oxygenated perfusion (HOPE) in the conservation of extended criteria donor (ECD) grafts from donation after brain death (DBD) donors during liver transplantation (LT). We performed a prospective, monocentric study (NCT03376074) designed to evaluate the interest of HOPE for ECD-DBD grafts. For comparison, a control group was selected after propensity score matching among patients who received transplants between 2010 and 2017. Between February and November 2018, the HOPE procedure was used in 25 LTs. Immediately after LT, the median aspartate aminotransferase (AST) level was significantly lower in the HOPE group (724UI versus 1284UI; P = 0.046) as were the alanine aminotransferase (ALT; 392UI versus 720UI; P = 0.01), lactate (2.2 versus 2.7; P = 0.01) There was a significant reduction in intensive care unit stay (3 versus 5 days; P = 0.01) and hospitalization (15 versus 20 days; P = 0.01). The incidence of early allograft dysfunction (EAD; 28% versus 42%; P = 0.22) was similar . A level of AST or ALT in perfusate >800UI was found to be highly predictive of EAD occurrence (areas under the curve, 0.92 and 0.91, respectively). The 12-month graft (88% versus 89.5%; P = 1.00) and patient survival rates (91% versus 91.3%; P = 1.00) were similar. The additional cost of HOPE was estimated at € 5298 per patient. The difference between costs and revenues, from the hospital's perspective, was not different between the HOPE and control groups (respectively, € 3023 versus € 4059]; IC, -€ 5470 and € 8652). HOPE may improve ECD graft function and reduce hospitalization stay without extra cost. These results must be confirmed in a randomized trial.
    MeSH term(s) Graft Survival ; Hospitalization ; Humans ; Liver/surgery ; Liver Transplantation/adverse effects ; Living Donors ; Organ Preservation ; Perfusion ; Prospective Studies ; Tissue Donors
    Language English
    Publishing date 2020-12-07
    Publishing country United States
    Document type Clinical Trial ; Journal Article
    ZDB-ID 2006866-9
    ISSN 1527-6473 ; 1527-6465
    ISSN (online) 1527-6473
    ISSN 1527-6465
    DOI 10.1002/lt.25955
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: High Intrapatient Variability of Tacrolimus Exposure in the Early Period After Liver Transplantation Is Associated With Poorer Outcomes.

    Rayar, Michel / Tron, Camille / Jézéquel, Caroline / Beaurepaire, Jean Marie / Petitcollin, Antoine / Houssel-Debry, Pauline / Camus, Christophe / Verdier, Marie Clémence / Dehlawi, Ammar / Lakéhal, Mohamed / Desfourneaux, Véronique / Meunier, Bernard / Sulpice, Laurent / Bellissant, Eric / Boudjema, Karim / Lemaitre, Florian

    Transplantation

    2018  Volume 102, Issue 3, Page(s) e108–e114

    Abstract: Background: Tacrolimus (TAC) is the cornerstone of immunosuppressive regimen in liver transplantation (LT). Its pharmacokinetics is characterized by a high interpatient and intrapatient variability (IPV) leading to an unpredictable dose-response ... ...

    Abstract Background: Tacrolimus (TAC) is the cornerstone of immunosuppressive regimen in liver transplantation (LT). Its pharmacokinetics is characterized by a high interpatient and intrapatient variability (IPV) leading to an unpredictable dose-response relationship. The aim of our study was to evaluate the impact of TAC IPV (IPV) on graft and patient outcomes after LT.
    Methods: We retrospectively analyzed 812 LT recipients treated with TAC. The IPV of TAC concentrations was estimated by calculating the coefficient of variation (CV) of whole blood trough concentrations. Patients were categorized in 2 groups: low IPV (CV < 40%) and high IPV (CV ≥ 40%).
    Results: There were significantly more neurologic complications (31.2% vs 16.6%, P < 0.001), cardiovascular complications (19.7% vs 9.7%, P < 0.001), and acute renal failure requiring dialysis (8.5% vs 2.2%, P < 0.001) in the high CV group than in the low CV group. Moreover, graft survival was significantly poorer in the high CV group (hazard ratio, 1.42; 95% confidence interval, 1.04-1.95; P = 0.03). A pretransplantation elevated Model for End-Stage Liver Disease score (P < 0.001) and Child-Pugh grade (P < 0.001) were identified as risk factors for presenting a high CV.
    Conclusions: A high CV of TAC concentrations was found to be predictive of TAC-related toxicity and poorer survival.
    MeSH term(s) Adolescent ; Adult ; Aged ; Female ; Graft Survival ; Humans ; Immunosuppressive Agents/therapeutic use ; Liver Transplantation/mortality ; Male ; Middle Aged ; Retrospective Studies ; Tacrolimus/blood ; Tacrolimus/therapeutic use ; Young Adult
    Chemical Substances Immunosuppressive Agents ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2018-02-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208424-7
    ISSN 1534-6080 ; 0041-1337
    ISSN (online) 1534-6080
    ISSN 0041-1337
    DOI 10.1097/TP.0000000000002052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Tacrolimus Concentrations Measured in Excreted Bile in Liver Transplant Recipients: The STABILE Study.

    Rayar, Michel / Tron, Camille / Locher, Clara / Chebaro, Alexandre / Beaurepaire, Jean-Marie / Blondeau, Marc / Cusumano, Caterina / Bardou-Jacquet, Edouard / Houssel-Debry, Pauline / Camus, Christophe / Petitcollin, Antoine / Verdier, Marie Clémence / Lakéhal, Mohamed / Desfourneaux, Véronique / Sulpice, Laurent / Meunier, Bernard / Bellissant, Eric / Boudjema, Karim / Lemaitre, Florian

    Clinical therapeutics

    2018  Volume 40, Issue 12, Page(s) 2088–2098

    Abstract: Purpose: Tacrolimus (TAC) is the main immunosuppressive drug in liver transplantation. Despite intensive therapeutic drug monitoring (TDM) that relies on whole blood trough concentration (TAC: Methods: The Therapeutic Drug Monitoring of Tacrolimus ... ...

    Abstract Purpose: Tacrolimus (TAC) is the main immunosuppressive drug in liver transplantation. Despite intensive therapeutic drug monitoring (TDM) that relies on whole blood trough concentration (TAC
    Methods: The Therapeutic Drug Monitoring of Tacrolimus Biliary Concentrations for Liver-Transplanted Patients (STABILE) study is a prospective monocentric trial. During the 7 first days after TAC therapy initiation, TAC
    Findings: Between May 2016 and April 2017, 41 patients were analyzed. TAC
    Implications: TAC
    MeSH term(s) Adult ; Aged ; Bile/metabolism ; Drug Monitoring/methods ; Female ; Hepatobiliary Elimination ; Humans ; Immunosuppressive Agents/pharmacokinetics ; Immunosuppressive Agents/therapeutic use ; Leukocytes, Mononuclear/metabolism ; Liver/metabolism ; Liver Transplantation ; Male ; Middle Aged ; Tacrolimus/pharmacokinetics ; Tacrolimus/therapeutic use
    Chemical Substances Immunosuppressive Agents ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2018-11-19
    Publishing country United States
    Document type Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 603113-4
    ISSN 1879-114X ; 0149-2918
    ISSN (online) 1879-114X
    ISSN 0149-2918
    DOI 10.1016/j.clinthera.2018.10.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A high performance liquid chromatography tandem mass spectrometry for the quantification of tacrolimus in human bile in liver transplant recipients.

    Tron, Camille / Rayar, Michel / Petitcollin, Antoine / Beaurepaire, Jean-Marie / Cusumano, Caterina / Verdier, Marie-Clémence / Houssel-Debry, Pauline / Camus, Christophe / Boudjema, Karim / Bellissant, Eric / Lemaitre, Florian

    Journal of chromatography. A

    2016  Volume 1475, Page(s) 55–63

    Abstract: Tacrolimus whole-blood concentrations imperfectly reflect concentrations at the effect site. Tacrolimus concentrations in the transplanted organ could be more relevant to predict rejection events. Because liver biopsy cannot be repeatedly performed after ...

    Abstract Tacrolimus whole-blood concentrations imperfectly reflect concentrations at the effect site. Tacrolimus concentrations in the transplanted organ could be more relevant to predict rejection events. Because liver biopsy cannot be repeatedly performed after liver transplantation, we suggested measuring tacrolimus in the bile to have a cost-effective and clinically implementable surrogate marker of intra-hepatic tacrolimus concentration. We developed and fully validated a liquid chromatography-tandem mass spectrometry method for the determination of tacrolimus in human bile. Sample purification was achieved using protein precipitation and liquid-liquid extraction with ethyl-acetate. Gradient elution was performed using a C18 analytical column with a 5min run-time. The method was linear from 0.5ng/mL to 20ng/mL. In this concentration range, within-day and between-day precisions as well as overall bias were within ±15%. Matrix effect was fully corrected by the internal standard (ascomycin). The assay was optimized to achieve good selectivity in this complex biological matrix. Tacrolimus was found to be stable in bile stored 6 months at -80°C, after 3 freeze and thaw cycles, 20h at room temperature and 24h in extracts kept at 15°C in the auto-sampler. The method was applied to quantify tacrolimus in bile from liver transplant recipients. It allowed getting preliminary data about tacrolimus excretion profile in bile and showed the lack of correlation between tacrolimus whole blood concentration and tacrolimus liver exposition. This alternative and innovative analytical approach of tacrolimus bio-analysis appears suitable for further studies evaluating relevance of biliary tacrolimus concentration as a new pharmacological marker of immunosuppressive activity.
    MeSH term(s) Bile/chemistry ; Chromatography, High Pressure Liquid/methods ; Humans ; Immunosuppressive Agents/analysis ; Immunosuppressive Agents/isolation & purification ; Liquid-Liquid Extraction ; Liver Transplantation ; Tacrolimus/analysis ; Tacrolimus/isolation & purification ; Tandem Mass Spectrometry/methods
    Chemical Substances Immunosuppressive Agents ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2016-12-02
    Publishing country Netherlands
    Document type Journal Article ; Validation Studies
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2016.10.075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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