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Article: Molecular Structural Analysis of Porcine CMAH-Native Ligand Complex and High Throughput Virtual Screening to Identify Novel Inhibitors.

Ogun, Oluwamayowa Joshua / Thaller, Georg / Becker, Doreen

2023 Volume 12, Issue 5

Abstract: Porcine meat is the most consumed red meat worldwide. Pigs are also vital tools in biological and medical research. However, xenoreactivity between porcine's N-glycolylneuraminic acid (Neu5Gc) and human anti-Neu5Gc antibodies poses a significant ... ...

Abstract	Porcine meat is the most consumed red meat worldwide. Pigs are also vital tools in biological and medical research. However, xenoreactivity between porcine's N-glycolylneuraminic acid (Neu5Gc) and human anti-Neu5Gc antibodies poses a significant challenge. On the one hand, dietary Neu5Gc intake has been connected to particular human disorders. On the other hand, some pathogens connected to pig diseases have a preference for Neu5Gc. The Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) catalyses the conversion of N-acetylneuraminic acid (Neu5Ac) to Neu5Gc. In this study, we predicted the tertiary structure of CMAH, performed molecular docking, and analysed the protein-native ligand complex. We performed a virtual screening from a drug library of 5M compounds and selected the two top inhibitors with Vina scores of -9.9 kcal/mol for inhibitor 1 and -9.4 kcal/mol for inhibitor 2. We further analysed their pharmacokinetic and pharmacophoric properties. We conducted stability analyses of the complexes with molecular dynamic simulations of 200 ns and binding free energy calculations. The overall analyses revealed the inhibitors' stable binding, which was further validated by the MMGBSA studies. In conclusion, this result may pave the way for future studies to determine how to inhibit CMAH activities. Further in vitro studies can provide in-depth insight into these compounds' therapeutic potential.
Language	English
Publishing date	2023-05-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12050684
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: An In Silico Functional Analysis of Non-Synonymous Single-Nucleotide Polymorphisms of Bovine

Ogun, Oluwamayowa Joshua / Soremekun, Opeyemi S / Thaller, Georg / Becker, Doreen

Pathogens (Basel, Switzerland)

2023 Volume 12, Issue 4

Abstract: The sugar molecule N-glycolylneuraminic acid (Neu5Gc) is one of the most common sialic acids discovered in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) catalyses the conversion of N-acetylneuraminic acid (Neu5Ac) to Neu5Gc, ... ...

Abstract	The sugar molecule N-glycolylneuraminic acid (Neu5Gc) is one of the most common sialic acids discovered in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) catalyses the conversion of N-acetylneuraminic acid (Neu5Ac) to Neu5Gc, and it is encoded by the
Language	English
Publishing date	2023-04-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12040591
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: An Overview of the Importance and Value of Porcine Species in Sialic Acid Research.

Ogun, Oluwamayowa Joshua / Thaller, Georg / Becker, Doreen

Biology

2022 Volume 11, Issue 6

Abstract: Humans frequently interact with pigs, whose meat is also one of the primary sources of animal protein. They are one of the main species at the center of sialic acid (Sia) research. Sias are sugars at terminals of glycoconjugates, are expressed at the ... ...

Abstract	Humans frequently interact with pigs, whose meat is also one of the primary sources of animal protein. They are one of the main species at the center of sialic acid (Sia) research. Sias are sugars at terminals of glycoconjugates, are expressed at the cell surfaces of mammals, and are important in cellular interactions. N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac) are notable Sias in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (
Language	English
Publishing date	2022-06-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology11060903
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: An Overview of the Importance and Value of Porcine Species in Sialic Acid Research

Ogun, Oluwamayowa Joshua / Thaller, Georg / Becker, Doreen

Biology. 2022 June 11, v. 11, no. 6

2022

Abstract	Humans frequently interact with pigs, whose meat is also one of the primary sources of animal protein. They are one of the main species at the center of sialic acid (Sia) research. Sias are sugars at terminals of glycoconjugates, are expressed at the cell surfaces of mammals, and are important in cellular interactions. N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac) are notable Sias in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) encodes the CMAH enzyme that biosynthesizes Neu5Gc. Although humans cannot endogenously synthesize Neu5Gc due to the inactivation of this gene by a mutation, Neu5Gc can be metabolically incorporated into human tissues from red meat consumption. Interactions between Neu5Gc and human anti-Neu5Gc antibodies have been associated with certain diseases and disorders. In this review, we summarized the sialic acid metabolic pathway, its regulation and link to viral infections, as well as the importance of the pig as a model organism in Sia research, making it a possible source of Neu5Gc antigens affecting human health. Future research in solving the structures of crucial enzymes involved in Sia metabolism, as well as their regulation and interactions with other enzymes, especially CMAH, could help to understand their function and reduce the amount of Neu5Gc.
Keywords	animal proteins ; biochemical pathways ; cytidine ; enzymes ; genes ; glycoconjugates ; human health ; humans ; meat consumption ; metabolism ; mutation ; red meat ; sialic acid ; swine
Language	English
Dates of publication	2022-0611
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology11060903
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Single-cell RNA sequencing of freshly isolated bovine milk cells and cultured primary mammary epithelial cells.

Becker, Doreen / Weikard, Rosemarie / Hadlich, Frieder / Kühn, Christa

Scientific data

2021 Volume 8, Issue 1, Page(s) 177

Abstract: Bovine mammary function at molecular level is often studied using mammary tissue or primary bovine mammary epithelial cells (pbMECs). However, bulk tissue and primary cells are heterogeneous with respect to cell populations, adding further ... ...

Abstract	Bovine mammary function at molecular level is often studied using mammary tissue or primary bovine mammary epithelial cells (pbMECs). However, bulk tissue and primary cells are heterogeneous with respect to cell populations, adding further transcriptional variation in addition to genetic background. Thus, understanding of the variation in gene expression profiles of cell populations and their effect on function are limited. To investigate the mononuclear cell composition in bovine milk, we analyzed a single-cell suspension from a milk sample. Additionally, we harvested cultured pbMECs to characterize gene expression in a homogeneous cell population. Using the Drop-seq technology, we generated single-cell RNA datasets of somatic milk cells and pbMECs. The final datasets after quality control filtering contained 7,119 and 10,549 cells, respectively. The pbMECs formed 14 indefinite clusters displaying intrapopulation heterogeneity, whereas the milk cells formed 14 more distinct clusters. Our datasets constitute a molecular cell atlas that provides a basis for future studies of milk cell composition and gene expression, and could serve as reference datasets for milk cell analysis.
MeSH term(s)	Animals ; Cattle ; Epithelial Cells/metabolism ; Female ; Gene Expression ; Leukocytes, Mononuclear/metabolism ; Mammary Glands, Animal/cytology ; Mammary Glands, Animal/metabolism ; Milk/cytology ; Milk/metabolism ; Primary Cell Culture ; Sequence Analysis, RNA ; Single-Cell Analysis
Language	English
Publishing date	2021-07-15
Publishing country	England
Document type	Dataset ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2775191-0
ISSN	2052-4463 ; 2052-4463
ISSN (online)	2052-4463
ISSN	2052-4463
DOI	10.1038/s41597-021-00972-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Color Trends and Selection for Product Design

Becker, Doreen

Every Color Sells A Story

(Plastics Design Library)

2016

Abstract: Color Trends and Selection for Product Design: Every Color Sells a Story speaks to the needs of the manufacturing level where colorants are developed, helping manufacturers to understand where their colors will sell and for what period of time these ... ...

Series title	Plastics Design Library
Abstract	Color Trends and Selection for Product Design: Every Color Sells a Story speaks to the needs of the manufacturing level where colorants are developed, helping manufacturers to understand where their colors will sell and for what period of time these products will be viable. It covers issues such as stability, color measurement, and new methods of incorporation, which are critical in the development of new colorants.The book helps product designers more effectively reach their target audiences by helping them understand more about how colors are chosen for particular markets and how certain colors will perform in designs, including how to evaluate color under different lighting conditions and in, or on, different materials. Knowing how colors will perform in each material and how they will be seen on a store shelf or show room floor is vital. The book gives an important insight into future trends, including new design methods for creating color prototypes and regulatory requirements. The color designer needs to better understand the world of the color formulator, and the formulator conversely needs to understand the needs of the designer, so this book is written for both.Provides an expert assessment of future trends in color, helping color manufacturers to understand how their customers and brand owners select colorsCovers the critical issues of stability, color measurement, and new methods of incorporation, helping engineers evaluate color performance in different designs, materials, and lighting conditionsHelps readers stay ahead of the competition with discussions of important regulations and trends in 'green' colors and product design Doreen is currently the Strategic Accounts Manager at A. Schulman Inc. (global plastics supplier), managing the Procter and Gamble Gillette account - responsible for color direction, trending and sales. She has previously worked as Market Development Manager for Americhem Inc and BASF (in packaging, automotive, durables, architectural and fibers markets), and spent 10 years as a Group Leader in the Plastics Applications and Design Lab at Engelhard Corporation - focusing on color design for plastics applications and color trend development using blow molding, injection molding and extrusion equipment. She has held the Chair at the Color Marketing Group, and is currently a board member of the Society of Plastics Engineers Color and Appearance Division
Language	English
Size	1 Online-Ressource (284 S.)
Edition	1. Aufl.
Document type	Book ; Online
Note	Description based upon print version of record
ISBN	0323393950 ; 9780323393959
Database	ECONomics Information System

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Article: Mitochondrial DNA Variation Contributes to the Aptitude for Dressage and Show Jumping Ability in the Holstein Horse Breed

Engel, Laura / Becker, Doreen / Nissen, Thomas / Russ, Ingolf / Thaller, Georg / Krattenmacher, Nina

Animals. 2022 Mar. 11, v. 12, no. 6

2022

Abstract: Maternal lineages are considered an important factor in breeding. Mitochondrial DNA (mtDNA) is maternally inherited and plays an important role in energy metabolism. It has already been associated with energy consumption and performances, e.g., stamina ... ...

Abstract	Maternal lineages are considered an important factor in breeding. Mitochondrial DNA (mtDNA) is maternally inherited and plays an important role in energy metabolism. It has already been associated with energy consumption and performances, e.g., stamina in humans and racehorses. For now, corresponding studies are lacking for sport performance of warmblood breeds. MtDNA sequences were available for 271 Holstein mares from 75 maternal lineages. As all mares within a lineage showed identical haplotypes regarding the non-synonymous variants, we expanded our data set by also including non-sequenced mares and assigning them to the lineage-specific haplotype. This sample consisting of 6334 to 16,447 mares was used to perform mitochondrial association analyses using breeding values (EBVs) estimated on behalf of the Fédération Équestre Nationale (FN) and on behalf of the Holstein Breeding Association (HOL). The association analyses revealed 20 mitochondrial SNPs (mtSNPs) significantly associated with FN-EBVs and partly overlapping 20 mtSNPs associated with HOL-EBVs. The results indicated that mtDNA contributes to performance differences between maternal lineages. Certain mitochondrial haplogroups were associated with special talents for dressage or show jumping. The findings encourage to set up innovative genetic evaluation models that also consider information on maternal lineages.
Keywords	Holstein ; data collection ; energy ; energy metabolism ; haplotypes ; mitochondria ; mitochondrial DNA ; sports
Language	English
Dates of publication	2022-0311
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12060704
Database	NAL-Catalogue (AGRICOLA)

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Article: Roan coat color in livestock

Voß, Katharina / Blaj, Iulia / Tetens, Julia L. / Thaller, Georg / Becker, Doreen

Animal genetics. 2022 Oct., v. 53, no. 5

2022

Abstract: Since domestication, a wide variety of phenotypes including coat color variation has developed in livestock. This variation is mostly based on selective breeding. During the beginning of selective breeding, potential negative consequences did not become ... ...

Abstract	Since domestication, a wide variety of phenotypes including coat color variation has developed in livestock. This variation is mostly based on selective breeding. During the beginning of selective breeding, potential negative consequences did not become immediately evident due to low frequencies of homozygous animals and have been occasionally neglected. However, numerous studies of coat color genetics have been carried out over more than a century and, meanwhile, pleiotropic effects for several coat color genes, including disorders of even lethal impact, were described. Similar coat color phenotypes can often be found across species, caused either by conserved genes or by different genes. Even in the same species, more than one gene could cause the same or similar coat color phenotype. The roan coat color in livestock species is characterized by a mixture of white and colored hair in cattle, pig, sheep, goat, alpaca, and horse. So far, the genetic background of this phenotype is not fully understood, but KIT and its ligand KITLG (MGF) are major candidate genes in livestock species. For some of these species, pleiotropic effects such as subfertility in homozygous roan cattle or homozygous embryonic lethality in certain horse breeds have been described. This review aims to point out the similarities and differences of the roan phenotype across the following livestock species: cattle, pig, sheep, goat, alpaca, and horse; and provides the current state of knowledge on genetic background and pleiotropic effects.
Keywords	alpacas ; animal genetics ; cattle ; color ; domestication ; embryonic mortality ; genes ; genetic background ; goats ; homozygosity ; horses ; ligands ; phenotype ; sheep ; swine
Language	English
Dates of publication	2022-10
Size	p. 549-556.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	REVIEW
ZDB-ID	632766-7
ISSN	1365-2052 ; 0268-9146 ; 0268-9154
ISSN (online)	1365-2052
ISSN	0268-9146 ; 0268-9154
DOI	10.1111/age.13240
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Coat Color Roan Shows Association with

Voß, Katharina / Tetens, Julia / Thaller, Georg / Becker, Doreen

Genes

2020 Volume 11, Issue 6

Abstract: Roan (Rn) horses show a typical seasonal change of color. Their body is covered with colored and white hair. We performed a descriptive statistical analysis of breeding records of Icelandic horses to challenge the hypothesis of roan being lethal in utero ...

Abstract	Roan (Rn) horses show a typical seasonal change of color. Their body is covered with colored and white hair. We performed a descriptive statistical analysis of breeding records of Icelandic horses to challenge the hypothesis of roan being lethal in utero under homozygous condition. The roan to non-roan ratio of foals from roan × roan matings revealed homozygous roan Icelandic horses to be viable. Even though roan is known to be inherited in a dominant mode and epistatic to other coat colors, the causative mutation is still unknown. Nevertheless, an association between roan phenotype and the
MeSH term(s)	Animals ; Breeding ; Color ; Genes, Lethal/genetics ; Hair Color/genetics ; Horse Diseases/genetics ; Horse Diseases/pathology ; Horses/genetics ; Mutation/genetics ; Phenotype ; Proto-Oncogene Proteins c-kit/genetics
Chemical Substances	Proto-Oncogene Proteins c-kit (EC 2.7.10.1)
Language	English
Publishing date	2020-06-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11060680
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Article: Coat Color Roan Shows Association with KIT Variants and No Evidence of Lethality in Icelandic Horses

Voß, Katharina / Tetens, Julia / Thaller, Georg / Becker, Doreen

Genes. 2020 June 22, v. 11, no. 6

2020

Abstract	Roan (Rn) horses show a typical seasonal change of color. Their body is covered with colored and white hair. We performed a descriptive statistical analysis of breeding records of Icelandic horses to challenge the hypothesis of roan being lethal in utero under homozygous condition. The roan to non-roan ratio of foals from roan × roan matings revealed homozygous roan Icelandic horses to be viable. Even though roan is known to be inherited in a dominant mode and epistatic to other coat colors, the causative mutation is still unknown. Nevertheless, an association between roan phenotype and the KIT gene was shown for different horse breeds. In the present study, we identified KIT variants by Sanger sequencing, and show that KIT is also associated with roan in the Icelandic horse breed.
Keywords	Icelandic (horse breed) ; breeding ; death ; epistasis ; foals ; genes ; homozygosity ; inheritance (genetics) ; mutation ; phenotype ; seasonal variation ; statistical analysis
Language	English
Dates of publication	2020-0622
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11060680
Database	NAL-Catalogue (AGRICOLA)

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