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  1. Article: From the § 64 LFGB MALDI-TOF working group: guidelines for validation of species identifications with MALDI-TOF-MS

    Rau, Jörg / Eisenberg, Tobias / Wind, Christine / Huber, Ingrid / Pavlovic, Melanie / Becker, René

    Journal für Verbraucherschutz und Lebensmittelsicherheit. 2022 Mar., v. 17, no. 1

    2022  

    Abstract: Matrix-assisted laser-desorption/ionization-time-of-flight-mass-spectrometry (MALDI-TOF-MS) is widely used to identify microorganisms. Recently, new applications such as identification of the animal species from meat, milk or fish are emerging. Standards ...

    Title translation Aus der § 64 LFGB-Arbeitsgruppe MALDI-TOF: Leitlinien für die Validierung von Spezies-Identifizierungen mittels MALDI-TOF-MS
    Abstract Matrix-assisted laser-desorption/ionization-time-of-flight-mass-spectrometry (MALDI-TOF-MS) is widely used to identify microorganisms. Recently, new applications such as identification of the animal species from meat, milk or fish are emerging. Standards for the validation of species identifications are still missing. Now, the § 64-LFGB working-group “MALDI-TOF”, established at the Federal Office of Consumer Protection and Food Safety, has compiled a guideline for the validation of species identifications. This guideline is intended for single laboratories as well as for lab networks and shows practical ways for validation of qualitative MALDI-TOF-MS methods. The special opportunities of the technology, in particular the use of extended reference databases and of collections of well-documented individual spectra for validation, have been taken into account in the guideline presented.
    Keywords consumer protection ; fish ; food safety ; guidelines ; meat ; milk
    Language English
    Dates of publication 2022-03
    Size p. 97-101.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 2232114-7
    ISSN 1661-5867 ; 1661-5751
    ISSN (online) 1661-5867
    ISSN 1661-5751
    DOI 10.1007/s00003-021-01353-x
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 7689: Show issues

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  2. Article: Trans

    Yıldız, Ayşegül / Hasani, Aida / Hempel, Tina / Köhl, Nina / Beicht, Aline / Becker, René / Hubich-Rau, Stefanie / Suchan, Martin / Poleganov, Marco A / Sahin, Ugur / Beissert, Tim

    Molecular therapy. Nucleic acids

    2024  Volume 35, Issue 2, Page(s) 102162

    Abstract: The co-delivery of microRNAs (miRNAs) and protein-coding RNA presents an opportunity for a combined approach to gene expression and gene regulation for therapeutic applications. Protein delivery is established using long mRNA, self-, ... ...

    Abstract The co-delivery of microRNAs (miRNAs) and protein-coding RNA presents an opportunity for a combined approach to gene expression and gene regulation for therapeutic applications. Protein delivery is established using long mRNA, self-, and
    Language English
    Publishing date 2024-03-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2662631-7
    ISSN 2162-2531
    ISSN 2162-2531
    DOI 10.1016/j.omtn.2024.102162
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  3. Article: Inter-laboratory Validation of an HPLC–MS/MS Method for the Detection of Microbial Transglutaminase in Meat and Meat Products

    Jira, Wolfgang / Behnke, Thomas / Brockmeyer, Jens / Frost, Kirstin / Hiller, Ekkehard / Möllers, Manfred / Niedzwiecka, Alicia / Pöpping, Bert / Uhlig, Steffen / Weidner, Markus / Wittke, Stefan / Becker, René

    Food analytical methods. 2022 Aug., v. 15, no. 8

    2022  

    Abstract: Microbial transglutaminase (TG) is an enzyme isolated on an industrial scale from Streptomyces mobaraensis. Technical TG, a formulated powder, is primarily used to restructure meat in the meat-processing industry, typically at a 1% concentration and is ... ...

    Abstract Microbial transglutaminase (TG) is an enzyme isolated on an industrial scale from Streptomyces mobaraensis. Technical TG, a formulated powder, is primarily used to restructure meat in the meat-processing industry, typically at a 1% concentration and is often referred to as “meat glue.” In the European Union, meat restructured with TG requires the indication “formed meat” on the label according to Regulation (EU) No 1169/2011. In order to detect food fraud like the undeclared TG usage in meat and meat products, a qualitative mass spectrometric method using specific tryptic marker peptides has been published in 2017. Here the successful inter-laboratory validation and first-time standardization of a proteomics method for food control is described, which was subsequently included into the Official Collection of Analysis Methods according to the German Food and Feed Code (§ 64 LFGB). Thirteen laboratories from governmental, academic, and private institutions participated in the study, whereas four laboratories did not meet the minimal quality criteria and therefore their results had to be excluded. Three different test materials containing between 0.2 and 2% technical TG as well as blank samples were produced and tested. The laboratories used triple-quadrupole mass spectrometers from several vendors as well as quadrupole time-of-flight instruments. The detection of TG was considered to be positive, if three mass transitions for the marker peptides VTPPAEPLDR (TG-1) and SPFYSALR (TG-2), each, showed a signal-to-noise ratio of at least 3. The level of detection LOD₉₅% for the median laboratory with intermediate performance was 0.31%, the false-positive rate was 0% and the false-negative rate was 2.1%.
    Keywords European Union ; Streptomyces mobaraensis ; food fraud ; food safety ; meat ; meat processing plants ; peptides ; protein-glutamine gamma-glutamyltransferase ; proteomics ; signal-to-noise ratio
    Language English
    Dates of publication 2022-08
    Size p. 2323-2334.
    Publishing place Springer US
    Document type Article
    ZDB-ID 2429656-9
    ISSN 1936-976X ; 1936-9751
    ISSN (online) 1936-976X
    ISSN 1936-9751
    DOI 10.1007/s12161-022-02289-0
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    In stock of ZB MED Bonn / Germany

    Z 7787: Show issues

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  4. Article ; Online: German Government Official Methods Board Points the Way Forward: Launch of a New Working Group for Mass Spectrometry for Protein Analysis to Detect Food Fraud and Food Allergens.

    Stoyke, Manfred / Becker, René / Brockmeyer, Jens / Jira, Wolfgang / Popping, Bert / Uhlig, Steffen / Wittke, Stefan

    Journal of AOAC International

    2019  Volume 102, Issue 5, Page(s) 1280–1285

    Abstract: The detection of food fraud and undeclared food allergens is one of the major challenges for competent authorities. Because adulterations are continuously adapted to the methods used to uncover them, the accomplishment of this task has become ... ...

    Abstract The detection of food fraud and undeclared food allergens is one of the major challenges for competent authorities. Because adulterations are continuously adapted to the methods used to uncover them, the accomplishment of this task has become increasingly difficult over time. In recent years, various new promising methods for the detection of multiple food adulterants and multiple food allergens have been developed. Some of them utilize LC-MS to identify specific marker peptides. However, these methods have yet to be validated and standardized. For this reason, the German officials have established a working group with the objective of validating methods through multilaboratory validation studies. The experts of the working group also aim for the first time to standardize validated methods and to develop general validation criteria. This manuscript will highlight the current work of the group. For this purpose, an overview is given on the principles and applications of the new mass spectrometric methods. Moreover, requirements and the present work of other institutions regarding method validation are described.
    MeSH term(s) Allergens/analysis ; Animals ; Chromatography, Liquid/methods ; Chromatography, Liquid/standards ; Food Contamination/analysis ; Germany ; Government Agencies ; Humans ; Laboratory Personnel ; Mass Spectrometry/methods ; Mass Spectrometry/standards ; Meat/analysis ; Plants/chemistry ; Proteins/analysis ; Shellfish/analysis ; Validation Studies as Topic
    Chemical Substances Allergens ; Proteins
    Language English
    Publishing date 2019-04-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 1103149-9
    ISSN 1944-7922 ; 1060-3271
    ISSN (online) 1944-7922
    ISSN 1060-3271
    DOI 10.5740/jaoacint.19-0056
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1229: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: A TCR-like CAR Promotes Sensitive Antigen Recognition and Controlled T-cell Expansion Upon mRNA Vaccination.

    Birtel, Matthias / Voss, Ralf-Holger / Reinhard, Katharina / Rengstl, Benjamin / Ouchan, Yasmina / Michel, Kristina / Hayduk, Nina / Tillmann, Bodo / Becker, René / Suchan, Martin / Theobald, Matthias / Oehm, Petra / Türeci, Özlem / Sahin, Ugur

    Cancer research communications

    2022  Volume 2, Issue 8, Page(s) 827–841

    Abstract: Chimeric antigen receptor (CAR) T cells are efficacious in patients with B-cell malignancies, while their activity is limited in patients with solid tumors. We developed a novel heterodimeric TCR-like CAR (TCAR) designed to achieve optimal chain pairing ... ...

    Abstract Chimeric antigen receptor (CAR) T cells are efficacious in patients with B-cell malignancies, while their activity is limited in patients with solid tumors. We developed a novel heterodimeric TCR-like CAR (TCAR) designed to achieve optimal chain pairing and integration into the T-cell CD3 signaling complex. The TCAR mediated high antigen sensitivity and potent antigen-specific T-cell effector functions in short-term
    Significance: A novel TCAR is tightly controlled by RNA vaccine-mediated costimulation and may provide an alternative to second-generation CARs for the treatment of solid tumors.
    MeSH term(s) Immunotherapy, Adoptive ; Humans ; T-Lymphocytes ; Receptors, Chimeric Antigen ; CD3 Complex ; Cell Proliferation ; mRNA Vaccines/immunology ; Neoplasms/therapy ; Cancer Vaccines/therapeutic use ; Animals ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Female ; Cell Line, Tumor ; Xenograft Model Antitumor Assays
    Chemical Substances Receptors, Chimeric Antigen ; CD3 Complex ; mRNA Vaccines ; Cancer Vaccines
    Language English
    Publishing date 2022-08-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2767-9764
    ISSN (online) 2767-9764
    DOI 10.1158/2767-9764.CRC-21-0154
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  6. Book ; Online ; Thesis: Quantitative Proteomanalyse des prädatorischen Bakteriums Bdellovibrio bacteriovorus

    Becker, René [Verfasser] / Linscheid, Michael W. [Gutachter] / Weller, Michael [Gutachter]

    2018  

    Author's details René Becker ; Gutachter: Michael W. Linscheid, Michael Weller
    Keywords Technische Chemie ; Technical Chemistry
    Subject code sg660
    Language German
    Publisher Humboldt-Universität zu Berlin
    Publishing place Berlin
    Document type Book ; Online ; Thesis
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  7. Article ; Online: Photocatalytic Hydrogen Evolution by a Synthetic [FeFe] Hydrogenase Mimic Encapsulated in a Porphyrin Cage.

    Nurttila, Sandra S / Becker, René / Hessels, Joeri / Woutersen, Sander / Reek, Joost N H

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2018  Volume 24, Issue 61, Page(s) 16395–16406

    Abstract: The design of a biomimetic and fully base metal photocatalytic system for photocatalytic proton reduction in a homogeneous medium is described. A synthetic pyridylphosphole-appended [FeFe] hydrogenase mimic was encapsulated inside a supramolecular zinc ... ...

    Abstract The design of a biomimetic and fully base metal photocatalytic system for photocatalytic proton reduction in a homogeneous medium is described. A synthetic pyridylphosphole-appended [FeFe] hydrogenase mimic was encapsulated inside a supramolecular zinc porphyrin-based metal-organic cage structure Fe
    Language English
    Publishing date 2018-10-09
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-X
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.201803351
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  8. Article ; Online: Photocatalytic Hydrogen Generation by Vesicle-Embedded [FeFe]Hydrogenase Mimics: A Mechanistic Study.

    Becker, René / Bouwens, Tessel / Schippers, Esther C F / van Gelderen, Toon / Hilbers, Michiel / Woutersen, Sander / Reek, Joost N H

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2019  Volume 25, Issue 61, Page(s) 13921–13929

    Abstract: Artificial photosynthesis-the direct photochemical generation of hydrogen from water-is a promising but scientifically challenging future technology. Because nature employs membranes for photodriven reactions, the aim of this work is to elucidate the ... ...

    Abstract Artificial photosynthesis-the direct photochemical generation of hydrogen from water-is a promising but scientifically challenging future technology. Because nature employs membranes for photodriven reactions, the aim of this work is to elucidate the effect of membranes on artificial photocatalysis. To do so, a combination of electrochemistry, photocatalysis, and time-resolved spectroscopy on vesicle-embedded [FeFe]hydrogenase mimics, driven by a ruthenium tris-2,2'-bipyridine photosensitizer, is reported. The membrane effects encountered can be summarized as follows: the presence of vesicles steers the reactivity of the [FeFe]-benzodithiolate catalyst towards disproportionation, instead of protonation, due to membrane characteristics, such as providing a constant local effective pH, and concentrating and organizing species inside the membrane. The maximum turnover number is limited by photodegradation of the resting state in the catalytic cycle. Understanding these fundamental productive and destructive pathways in complex photochemical systems allows progress towards the development of efficient artificial leaves.
    Language English
    Publishing date 2019-09-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-X
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.201902514
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  9. Article ; Online: Software assisted data analysis for relative quantification of differentially metal labeled proteins based on HPLC/ESI-MS and -MS/MS experiments.

    Becker, René / Schwarz, Gunnar / Beck, Sebastian / Linscheid, Michael W

    Journal of mass spectrometry : JMS

    2015  Volume 50, Issue 10, Page(s) 1120–1123

    Language English
    Publishing date 2015-10
    Publishing country England
    Document type Letter
    ZDB-ID 1221763-3
    ISSN 1096-9888 ; 1076-5174
    ISSN (online) 1096-9888
    ISSN 1076-5174
    DOI 10.1002/jms.3627
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4254: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 220: Show issues

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  10. Article ; Online: Identification and Characterization of Differentially-Regulated Type IVb Pilin Genes Necessary for Predation in Obligate Bacterial Predators.

    Avidan, Ofir / Petrenko, Margarita / Becker, René / Beck, Sebastian / Linscheid, Michael / Pietrokovski, Shmuel / Jurkevitch, Edouard

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 1013

    Abstract: Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still ... ...

    Abstract Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still poorly detailed. By combining omics data available for Bdellovibrio and like organisms (BALO's), we identified 43 genes expressed in B. bacteriovorus during the early interaction with prey. These included genes in a tight adherence (TAD) operon encoding for two type IVb fimbriae-like pilin proteins (flp1 and flp2), and their processing and export machinery. Two additional flp genes (flp3 and flp4) were computationally identified at other locations along the chromosome, defining the largest and most diverse type IVb complement known in bacteria to date. Only flp1, flp2 and flp4 were expressed; their respective gene knock-outs resulted in a complete loss of the predatory ability without losing the ability to adhere to prey cells. Additionally, we further demonstrate differential regulation of the flp genes as the TAD operon of BALOs with different predatory strategies is controlled by a flagellar sigma factor FliA, while flp4 is not. Finally, we show that FliA, a known flagellar transcriptional regulator in other bacteria, is an essential Bdellovibrio gene.
    MeSH term(s) Bacterial Adhesion ; Bacterial Proteins/genetics ; Bdellovibrio bacteriovorus/genetics ; Bdellovibrio bacteriovorus/physiology ; Computational Biology/methods ; Fimbriae Proteins/genetics ; Fimbriae, Bacterial/genetics ; Fimbriae, Bacterial/metabolism ; Gene Expression Regulation, Bacterial ; Genes, Essential ; Genomics
    Chemical Substances Bacterial Proteins ; Fimbriae Proteins (147680-16-8)
    Language English
    Publishing date 2017-04-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-00951-w
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