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  1. Article: Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates

    Prathiviraj, Ragothaman / Chellapandi, Paulchamy / Begum, Ajima / Kiran, George Seghal / Selvin, Joseph

    Virus research. 2022 Jan. 02, v. 307

    2022  

    Abstract: The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic ... ...

    Abstract The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic variants. Brazil is the world's second-most COVID-19 affected country. In the present study, we identified the genomic and proteomic variants of Brazilian SARS-CoV-2 isolates. We identified 16 different genotypic variants were found among the 27 isolates. The genotypes of three isolates such as Bra/1236/2021 (G15), Bra/MASP2C844R2/2020 (G11), and Bra/RJ-DCVN5/2020 (G9) have a unique mutant in NSP4 (S184N), 2′O-Mutase (R216N), membrane protein (A2V) and Envelope protein (V5A). A mutation in RdRp of SARS-CoV-2, particularly the change of Pro-to Leu-at 323 resulted in the stabilization of the structure in BRA/CD1739-P4/2020. NSP4, NSP5 protein mutants are more virulent in genotype 15 and 16. A fast protein folding rate changes the structural stability and leads to escape for current antivirals. Thus, our findings help researchers to develop the best potent antivirals based on the new mutant of Brazilian isolates.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; antiviral agents ; genomics ; genotype ; membrane proteins ; mutants ; mutation ; proteomics ; research ; virulence ; viruses ; Brazil
    Language English
    Dates of publication 2022-0102
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2021.198618
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  2. Article: The impact of Achyranthes aspera seeds and leaves supplemented feeds on the survival, growth, immune system and specific genes involved in immunostimulation in Clarias batrachus fry challenged with Aeromonas hydrophila in pond conditions

    Sharma, JaiGopal / Singh, Amarjeet / Begum, Ajima / Sonia / Krishna, Vungarala Hari / Chakrabarti, Rina

    Fish & shellfish immunology. 2021 Nov., v. 118

    2021  

    Abstract: The present study was conducted to evaluate the effect of dietary inclusion of Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. Magur fry (0.51 ± 0.032 g) were ... ...

    Abstract The present study was conducted to evaluate the effect of dietary inclusion of Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. Magur fry (0.51 ± 0.032 g) were cultured in hapas set inside a pond and were fed with three feeds. Two experimental feeds FS1 and FS2 were supplemented with 0.5% seeds and leaves of A. aspera, respectively and FC3 was the control one. After 90 days of feeding, fish were challenged with A. hydrophila. In FC3, 70% fish died within 48 h of challenge, while 25 and 30% mortality were recorded in FS1 and FL2, respectively. The cumulative mortality rates were 70, 45 and 35% in FC3, FL2 and FS1, respectively. The average weight and specific growth rate of magur were significantly higher in FS1 compared to others. Serum lysozyme, myeloperoxidase, nitric oxide synthase and superoxide dismutase levels were significantly higher in FS1 compared to others. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly lower in FS1 compared to others. In liver and head kidney of FS1 and FS2 fed magur, the iNOS, SOD-C, TNF-α, Cytochrome c, Caspase 9 were up-regulated. Caspase 3 was also significantly up-regulated in FS1 and it was followed by FL2 treatment. A. aspera incorporated feeds improved the immune system of fish and gave protection against bacteria even in the pond conditions.
    Keywords Achyranthes aspera ; Aeromonas hydrophila ; Clarias batrachus ; blood serum ; caspase-3 ; caspase-9 ; immune system ; kidneys ; liver ; lysozyme ; mortality ; myeloperoxidase ; shellfish ; specific growth rate ; superoxide dismutase
    Language English
    Dates of publication 2021-11
    Size p. 11-18.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2021.08.026
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.

    Prathiviraj, Ragothaman / Chellapandi, Paulchamy / Begum, Ajima / Kiran, George Seghal / Selvin, Joseph

    Virus research

    2021  Volume 307, Page(s) 198618

    Abstract: The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic ... ...

    Abstract The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic variants. Brazil is the world's second-most COVID-19 affected country. In the present study, we identified the genomic and proteomic variants of Brazilian SARS-CoV-2 isolates. We identified 16 different genotypic variants were found among the 27 isolates. The genotypes of three isolates such as Bra/1236/2021 (G15), Bra/MASP2C844R2/2020 (G11), and Bra/RJ-DCVN5/2020 (G9) have a unique mutant in NSP4 (S184N), 2'O-Mutase (R216N), membrane protein (A2V) and Envelope protein (V5A). A mutation in RdRp of SARS-CoV-2, particularly the change of Pro-to Leu-at 323 resulted in the stabilization of the structure in BRA/CD1739-P4/2020. NSP4, NSP5 protein mutants are more virulent in genotype 15 and 16. A fast protein folding rate changes the structural stability and leads to escape for current antivirals. Thus, our findings help researchers to develop the best potent antivirals based on the new mutant of Brazilian isolates.
    MeSH term(s) Brazil ; COVID-19/pathology ; Coronavirus 3C Proteases/genetics ; Coronavirus Nucleocapsid Proteins/genetics ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Genetic Variation/genetics ; Genome, Viral/genetics ; Humans ; Phosphoproteins/genetics ; Protein Folding ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Spike Glycoprotein, Coronavirus/genetics ; Viral Nonstructural Proteins/genetics ; Virulence/genetics
    Chemical Substances Coronavirus Nucleocapsid Proteins ; NSP4 protein, SARS-CoV-2 ; Phosphoproteins ; Spike Glycoprotein, Coronavirus ; Viral Nonstructural Proteins ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2 ; Coronavirus RNA-Dependent RNA Polymerase (EC 2.7.7.48) ; NSP12 protein, SARS-CoV-2 (EC 2.7.7.48) ; 3C-like proteinase, SARS-CoV-2 (EC 3.4.22.-) ; Coronavirus 3C Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2021-11-03
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2021.198618
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: The impact of Achyranthes aspera seeds and leaves supplemented feeds on the survival, growth, immune system and specific genes involved in immunostimulation in Clarias batrachus fry challenged with Aeromonas hydrophila in pond conditions.

    Sharma, JaiGopal / Singh, Amarjeet / Begum, Ajima / Sonia / Krishna, Vungarala Hari / Chakrabarti, Rina

    Fish & shellfish immunology

    2021  Volume 118, Page(s) 11–18

    Abstract: The present study was conducted to evaluate the effect of dietary inclusion of Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. Magur fry (0.51 ± 0.032 g) were ... ...

    Abstract The present study was conducted to evaluate the effect of dietary inclusion of Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. Magur fry (0.51 ± 0.032 g) were cultured in hapas set inside a pond and were fed with three feeds. Two experimental feeds FS1 and FS2 were supplemented with 0.5% seeds and leaves of A. aspera, respectively and FC3 was the control one. After 90 days of feeding, fish were challenged with A. hydrophila. In FC3, 70% fish died within 48 h of challenge, while 25 and 30% mortality were recorded in FS1 and FL2, respectively. The cumulative mortality rates were 70, 45 and 35% in FC3, FL2 and FS1, respectively. The average weight and specific growth rate of magur were significantly higher in FS1 compared to others. Serum lysozyme, myeloperoxidase, nitric oxide synthase and superoxide dismutase levels were significantly higher in FS1 compared to others. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly lower in FS1 compared to others. In liver and head kidney of FS1 and FS2 fed magur, the iNOS, SOD-C, TNF-α, Cytochrome c, Caspase 9 were up-regulated. Caspase 3 was also significantly up-regulated in FS1 and it was followed by FL2 treatment. A. aspera incorporated feeds improved the immune system of fish and gave protection against bacteria even in the pond conditions.
    MeSH term(s) Achyranthes ; Aeromonas hydrophila ; Animals ; Catfishes/immunology ; Catfishes/microbiology ; Fish Diseases ; Gram-Negative Bacterial Infections/veterinary ; Immune System ; Immunization ; Plant Leaves ; Ponds
    Language English
    Publishing date 2021-08-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2021.08.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 5917: Show issues

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  5. Article: Rediscovery of Syzygium cyanophyllum (Myrtaceae): a threatened rheophytic shrub endemic to Assam, north-east India

    Sarma, Jatindra / Barbhuiya, Hussain A / Dey, Santanu / Begum, Ajima

    Webbia. 2019 July 3, v. 74, no. 2

    2019  

    Abstract: Syzygium cyanophyllum is rediscovered after a gap of 103 years from Assam, north-east India. Here we complement the morphologic knowledge of the species by providing detailed taxonomic description with the first photographs of living plants. Its ... ...

    Abstract Syzygium cyanophyllum is rediscovered after a gap of 103 years from Assam, north-east India. Here we complement the morphologic knowledge of the species by providing detailed taxonomic description with the first photographs of living plants. Its taxonomic relationships with other species of genus Syzygium are also examined and discussed. Recent surveys confirm that the habitat and regional populations of the plant are under threat. Hence, following IUCN Red List Categories and Criteria it is categorised as Endangered.
    Keywords Syzygium ; habitats ; indigenous species ; photographs ; shrubs ; surveys ; taxon descriptions ; threatened species ; India
    Language English
    Dates of publication 2019-0703
    Size p. 301-305.
    Publishing place Taylor & Francis
    Document type Article
    ISSN 2169-4060
    DOI 10.1080/00837792.2019.1675261
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Growth enhancement in marine copepod, Pseudodiaptomus annandalei fed with the sodium acetate containing N/P starved medium grown, Tetraselmis suecica

    Dinesh Kumar, Sundarraj / Krishnaveni, Nagarajan / Santhanam, Perumal / Raju, Piliyan / Perumal, Pachiyappan / Begum, Ajima / Ahmed, Shahaj Uddin / Pragnya, Mangalagiri / Dhanalakshmi, Balasubramanian / Kim, Mi‐Kyung

    Aquaculture research. 2021 Sept., v. 52, no. 9

    2021  

    Abstract: Algal lipids are considered to be good raw materials towards aqua feed formulation and biofuels production, and hence, they have been investigated for decades. Using external carbon source, microalgal cultivation has been getting attention due to high ... ...

    Abstract Algal lipids are considered to be good raw materials towards aqua feed formulation and biofuels production, and hence, they have been investigated for decades. Using external carbon source, microalgal cultivation has been getting attention due to high biomass productivity and lipid content with flexible cultivation of algae. In this present study, a marine microalga, Tetraselmis suecica was cultivated by combined method with sodium acetate as carbon source and reciprocal nutrients concentration. The reciprocal concentration of nitrogen and phosphorus with sodium acetate supply towards lipid and fatty acid methyl esters (FAME) synthesis in terms of live feed efficiency was attempted. The highest FAME content (78.44 ± 1.18% of DW) of alga was obtained in nil nitrogen condition with limited phosphate concentration (N0‐PL) followed by N0‐P (77.31 ± 2.5% of DW) combination. Limitation of phosphate with combination of nil nitrogen (N0‐PL) showed slight increment of total FAME accumulation and its higher increments resulted in mono‐unsaturated fatty acids (MUFA) accumulation, indicating nitrogen starvation was a real FAME trigger under heterotrophic cultivation. Effect of heterotrophic cultivated T. suecica feeding on marine copepod Pseudodiaptomus annandalei produced higher population density (119 ± 15 ind. L⁻¹) in N0‐P combination and high rate (71.98%) of nauplii production was obtained in N0‐PL combination. These findings suggested that the heterotrophic microalgae cultivation can be practiced for aqua feed (especially for copepod cultivations) formulation as well as to phosphorous removal from wastewaters.
    Keywords Copepoda ; Tetraselmis suecica ; aquaculture ; biofuels ; biomass production ; carbon ; feed conversion ; feed formulation ; lipid content ; live feeds ; microalgae ; nauplii ; nitrogen ; nitrogen content ; phosphates ; phosphorus ; population density ; research ; sodium acetate ; starvation ; wastewater
    Language English
    Dates of publication 2021-09
    Size p. 4154-4165.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1227359-4
    ISSN 1355-557X
    ISSN 1355-557X
    DOI 10.1111/are.15254
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    In stock of ZB MED Bonn / Germany

    Z 4230: Show issues

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  7. Article: Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies

    Suryakodi, S / Majeed, S. Abdul / Taju, G / Vimal, S / Sivakumar, S / Ahmed, A. Nafeez / Shah, Feroz Ahmad / Bhat, Saqib Ahmad / Sarma, Dandadhar / Begum, Ajima / Hameed, A.S. Sahul

    Aquaculture. 2021 Feb. 15, v. 532

    2021  

    Abstract: Two novel cell lines were developed from eye and kidney of rainbow trout, Oncorhynchus mykiss in addition to another cell line from spleen. All these cell lines were characterized and cryopreserved successfully at different passage levels. The cells of ... ...

    Abstract Two novel cell lines were developed from eye and kidney of rainbow trout, Oncorhynchus mykiss in addition to another cell line from spleen. All these cell lines were characterized and cryopreserved successfully at different passage levels. The cells of eye, kidney and spleen were sub-cultured continuously at an interval of 2–3 days and attained the passage level of 112, 106 and 112, respectively over a period of 2 years. These cell lines consisted of both fibroblastic and epithelial like cells, and showed the presence of pancytokeratin and fibronectin by immunofluorescence. Maximum growth of cells was observed at 16 °C and 15% concentration of FBS. Amplification and sequence analysis of COI of O. mykiss confirmed the origin of these cell lines from O. mykiss. These cell lines were found to be highly susceptible to fish nodavirus (FNV) and tilapia lake virus (TiLV), and resistant to Cyprinid herpesvirus 2 (CyHV-2). The RT-PCR assay confirmed the infection of TiLV and FNV in infected cell lines. The FNV infection was further confirmed by Western blot and ELISA. The titre value of FNV in infected RTE, RTS and RTK cells gradually increased from 10³.², 10².⁹, 10³.³ TCID₅₀ ml⁻¹ at 2 d.p.i, to 10⁸.², 10⁷.⁹, 10⁸.⁸ TCID₅₀ ml⁻¹ at 10 d.p.i. respectively. Attempt was made to use these cell lines for virus isolation from disease suspected fish samples collected from different districts of Assam state, India.
    Keywords Cyprinid herpesvirus 2 ; Nodaviridae ; Oncorhynchus mykiss ; Tilapia lake virus ; Western blotting ; cell growth ; cell lines ; cryopreservation ; enzyme-linked immunosorbent assay ; epithelium ; eyes ; fibronectins ; fish ; fluorescent antibody technique ; kidneys ; reverse transcriptase polymerase chain reaction ; sequence analysis ; spleen ; viruses ; India
    Language English
    Dates of publication 2021-0215
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2020.736027
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3275: Show issues

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