Article ; Online: Diagnosis of thrombotic thrombocytopenic purpura: Easy-to-use fiber-optic surface plasmon resonance immunoassays for automated ADAMTS13 antigen and conformation evaluation.
Journal of thrombosis and haemostasis : JTH
2024
Abstract: Background: Laboratory diagnosis of immune-mediated thrombotic thrombocytopenic purpura (iTTP) remains challenging when ADAMTS13 activity ranges between 10-20%. To prevent misdiagnosis, open ADAMTS13 conformation gained clinical attention as a novel ... ...
| Abstract | Background: Laboratory diagnosis of immune-mediated thrombotic thrombocytopenic purpura (iTTP) remains challenging when ADAMTS13 activity ranges between 10-20%. To prevent misdiagnosis, open ADAMTS13 conformation gained clinical attention as a novel biomarker especially to diagnose acute iTTP in patient with diagnostic undecisive ADAMTS13 activity. Plasma ADAMTS13 conformation analysis corrects for ADAMTS13 antigen with both parameters being characterized in enzyme-linked immunosorbent assay (ELISA)-based reference assays requiring expert technicians. Objectives: To design ADAMTS13 antigen and conformation assays on automated, easy-to-use fiber-optic surface plasmon resonance (FO-SPR) technology to promote assay accessibility and diagnose challenging iTTP patients. Patients/methods: ADAMTS13 antigen and conformation assays were designed on FO-SPR technology. Plasma of twenty healthy donors and twenty acute iTTP patients were quantified and data from FO-SPR and ELISA reference assays were compared. Results: Following assay design, both antigen and conformation FO-SPR assays were optimized and characterized presenting strong analytical sensitivity (detection limit of 0.001 μg/mL) and repeatability (inter-assay variation of 14.4%). Comparative analysis suggests positive correlation (Spearman r of 0.92) and good agreement between FO-SPR and ELISA assays. As expected, FO-SPR assays showed a closed or open ADAMTS13 conformation in healthy donors and acute iTTP patients respectively. Conclusions: Both ADAMTS13 antigen and conformation assays were transferred onto automated, easy-to-use FO-SPR technology displaying potent analytical sensitivity and reproducibility. ADAMTS13 antigen and conformation were determined for healthy donors and acute iTTP patients showing strong correlation with ELISA reference. Introducing FO-SPR technology in clinical context could support routine diagnosis of acute iTTP patients, notably when ADAMTS13 activity fluctuates between 10-20%. |
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| Language | English | ||||||||||
| Publishing date | 2024-03-28 | ||||||||||
| Publishing country | England | ||||||||||
| Document type | Journal Article | ||||||||||
| ZDB-ID | 2112661-6 | ||||||||||
| ISSN | 1538-7836 ; 1538-7933 | ||||||||||
| ISSN (online) | 1538-7836 | ||||||||||
| ISSN | 1538-7933 | ||||||||||
| DOI | 10.1016/j.jtha.2024.03.012 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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