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  1. Article ; Online: High throughput multiplex-PCR for direct detection and diagnosis of dermatophyte species, Candida albicans and Candida parapsilosis in clinical specimen.

    Vahidnia, Ali / Bekers, Wouter / Bliekendaal, Harry / Spaargaren, Joke

    Journal of microbiological methods

    2015  Volume 113, Page(s) 38–40

    Abstract: We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid ... ...

    Abstract We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid diagnosis by our m-PCR, clinicians are able to initiate adequate antimycotic therapy much earlier.
    MeSH term(s) Arthrodermataceae/genetics ; Arthrodermataceae/isolation & purification ; Candida/genetics ; Candida/isolation & purification ; Candida albicans/genetics ; Candida albicans/isolation & purification ; Candidiasis, Cutaneous/diagnosis ; DNA Primers ; DNA, Fungal/isolation & purification ; Hair/microbiology ; High-Throughput Nucleotide Sequencing ; Humans ; Multiplex Polymerase Chain Reaction/methods ; Nails/microbiology ; Sensitivity and Specificity ; Sequence Alignment ; Skin
    Chemical Substances DNA Primers ; DNA, Fungal
    Language English
    Publishing date 2015-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2015.03.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex.

    van den Broek, Theo / Janssen, Nard G / Hetem, David J / Bekers, Wouter / Kamst, Miranda / Fluit, Ad C / van Ingen, Jakko / Kusters, Johannes G / Rentenaar, Rob J

    Diagnostic microbiology and infectious disease

    2019  Volume 95, Issue 3, Page(s) 114858

    Abstract: Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as ...

    Abstract Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.
    MeSH term(s) Adult ; Animals ; Cattle ; Diagnostic Errors ; Humans ; Lymphadenitis/diagnosis ; Lymphadenitis/microbiology ; Lymphadenitis/pathology ; Lymphadenitis/therapy ; Male ; Microbial Sensitivity Tests ; Molecular Diagnostic Techniques/methods ; Molecular Diagnostic Techniques/standards ; Mycobacterium Infections, Nontuberculous/diagnosis ; Mycobacterium Infections, Nontuberculous/microbiology ; Mycobacterium Infections, Nontuberculous/pathology ; Mycobacterium Infections, Nontuberculous/surgery ; Mycobacterium fortuitum/chemistry ; Mycobacterium fortuitum/classification ; Mycobacterium fortuitum/genetics ; Mycobacterium smegmatis/chemistry ; Mycobacterium smegmatis/classification ; Mycobacterium smegmatis/genetics ; Reagent Kits, Diagnostic ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Treatment Outcome
    Chemical Substances Reagent Kits, Diagnostic
    Language English
    Publishing date 2019-06-24
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2019.06.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1845: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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