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  1. Article ; Online: Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.

    Rubio, Angela / García-Blanco, Natalia / Vázquez-Bolado, Alicia / Belén Suárez, María / Moreno, Sergio

    Journal of cell science

    2018  Volume 131, Issue 18

    Abstract: In nature, cells and in particular unicellular microorganisms are exposed to a variety of nutritional environments. Fission yeast cells cultured in nitrogen-rich media grow fast, divide with a large size and show a short G1 and a long G2. However, when ... ...

    Abstract In nature, cells and in particular unicellular microorganisms are exposed to a variety of nutritional environments. Fission yeast cells cultured in nitrogen-rich media grow fast, divide with a large size and show a short G1 and a long G2. However, when cultured in nitrogen-poor media, they exhibit reduced growth rate and cell size and a long G1 and a short G2. In this study, we compared the phenotypes of cells lacking the highly conserved cyclin-dependent kinase (Cdk) inhibitor Rum1 and the anaphase-promoting complex/cyclosome (APC/C) activator Ste9 in nitrogen-rich and nitrogen-poor media. Rum1 and Ste9 are dispensable for cell division in nitrogen-rich medium. However, in nitrogen-poor medium they are essential for generating a proper wave of MluI cell-cycle box binding factor (MBF)-dependent transcription at the end of G1, which is crucial for promoting a successful S phase. Mutants lacking Rum1 and Ste9 showed premature entry into S phase and a reduced wave of MBF-dependent transcription, leading to replication stress, DNA damage and G2 cell cycle arrest. This work demonstrates how reprogramming the cell cycle by changing the nutritional environment may reveal new roles for cell cycle regulators.
    MeSH term(s) CDC2 Protein Kinase/metabolism ; Cell Cycle ; Cellular Reprogramming/physiology ; Fungal Proteins/metabolism ; Schizosaccharomyces/metabolism
    Chemical Substances Fungal Proteins ; CDC2 Protein Kinase (EC 2.7.11.22)
    Language English
    Publishing date 2018-09-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.218743
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article: A physiological and biochemical approach to the systematics of Colletotrichum species pathogenic to strawberry

    Garcia Munoz, J.A / Belen Suarez, M / Grondona, I / Monte, E / Buddie, A.G / Bridge, P.D / Cannon, P.F

    Mycologia. May/June 2000. v. 92 (3)

    2000  

    Keywords Colletotrichum ; Glomerella ; Fragaria ananassa ; taxonomy ; plant pathogenic fungi ; strain differences ; numerical taxonomy ; chemotaxonomy ; physiology ; biochemistry ; fungal diseases of plants
    Language English
    Dates of publication 2000-05
    Size p. 488-498.
    Document type Article
    ZDB-ID 281335-x
    ISSN 1557-2536 ; 0027-5514
    ISSN (online) 1557-2536
    ISSN 0027-5514
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans

    Gutierrez-Escribano, Pilar / Zeidler, Ute / Belen Suarez, M. / Bachellier-Bassi, Sophie / Clemente-Blanco, Andres / Bonhomme, Julie / Vazquez de Aldana, Carlos R. / d'Enfert, Christophe / Correa-Bordes, Jaime

    Plos Pathogens 5 (8), . (2012)

    Abstract: In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based ... ...

    Abstract In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability to establish biofilms. The transcription factor Bcr1 is a master regulator of C. albicans biofilm development, although the full extent of its regulation remains unknown. Here, we report that Bcr1 is a phosphoprotein that physically interacts with the NDR kinase Cbk1 and undergoes Cbk1-dependent phosphorylation. Mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to alanine markedly impaired Bcr1 function during biofilm formation and virulence in a mouse model of disseminated candidiasis. Cells lacking Cbk1, or any of its upstream activators, also had reduced biofilm development. Notably, mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to glutamate in cbk1 Delta cells upregulated the transcription of Bcr1-dependent genes and partially rescued the biofilm defects of a cbk1 Delta strain. Therefore, our data uncovered a novel role of the NDR/LATS kinase Cbk1 in the regulation of biofilm development through the control of Bcr1.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  4. Article: The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans

    Gutierrez-Escribano, Pilar / Zeidler, Ute / Belen Suarez, M. / Bachellier-Bassi, Sophie / Clemente-Blanco, Andres / Bonhomme, Julie / Vazquez de Aldana, Carlos R. / d'Enfert, Christophe / Correa-Bordes, Jaime

    Plos Pathogens 5 (8), . (2012)

    Abstract: In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based ... ...

    Abstract In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability to establish biofilms. The transcription factor Bcr1 is a master regulator of C. albicans biofilm development, although the full extent of its regulation remains unknown. Here, we report that Bcr1 is a phosphoprotein that physically interacts with the NDR kinase Cbk1 and undergoes Cbk1-dependent phosphorylation. Mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to alanine markedly impaired Bcr1 function during biofilm formation and virulence in a mouse model of disseminated candidiasis. Cells lacking Cbk1, or any of its upstream activators, also had reduced biofilm development. Notably, mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to glutamate in cbk1 Delta cells upregulated the transcription of Bcr1-dependent genes and partially rescued the biofilm defects of a cbk1 Delta strain. Therefore, our data uncovered a novel role of the NDR/LATS kinase Cbk1 in the regulation of biofilm development through the control of Bcr1.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  5. Article: A single nucleotide polymorphism uncovers a novel function for the transcription factor Ace2 during Candida albicans hyphal development

    Calderón-Noreña, Diana M. / González-Novo, Alberto / Orellana-Muñoz, Sara / Gutiérrez-Escribano, Pilar / Arnáiz-Pita, Yolanda / Dueñas-Santero, Encarnación / Belén Suárez, M. / Bougnoux, Marie-Elisabeth / del Rey, Francisco / Sherlock, Gavin / D'Enfert, Christophe / Correa-Bordes, Jaime / Vázquez de Aldana, Carlos R.

    Plos Genetics 4 (11), . (2015)

    Abstract: Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for ... ...

    Abstract Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for filamentous growth is the ability to inhibit cell separation after cytokinesis, although it is poorly understood how this process is regulated developmentally. In C. albicans, the formation of filaments during hyphal growth requires changes in septin ring dynamics. In this work, we studied the functional relationship between septins and the transcription factor Ace2, which controls the expression of enzymes that catalyze septum degradation. We found that alternative translation initiation produces two Ace2 isoforms. While full-length Ace2, Ace2L, influences septin dynamics in a transcription-independent manner in hyphal cells but not in yeast cells, the use of methionine-55 as the initiation codon gives rise to Ace2S, which functions as the nuclear transcription factor required for the expression of cell separation genes. Genetic evidence indicates that Ace2L influences the incorporation of the Sep7 septin to hyphal septin rings in order to avoid inappropriate activation of cell separation during filamentous growth. Interestingly, a natural single nucleotide polymorphism (SNP) present in the C. albicans WO-1 background and other C. albicans commensal and clinical isolates generates a stop codon in the ninth codon of Ace2L that mimics the phenotype of cells lacking Ace2L. Finally, we report that Ace2L and Ace2S interact with the NDR kinase Cbk1 and that impairing activity of this kinase results in a defect in septin dynamics similar to that of hyphal cells lacking Ace2L. Together, our findings identify Ace2L and the NDR kinase Cbk1 as new elements of the signaling system that modify septin ring dynamics in hyphae to allow cell-chain formation, a feature that appears to have evolved in specific C. albicans lineages.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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    Inter-library loan at ZB MED

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  6. Article: A single nucleotide polymorphism uncovers a novel function for the transcription factor Ace2 during Candida albicans hyphal development

    Calderón-Noreña, Diana M. / González-Novo, Alberto / Orellana-Muñoz, Sara / Gutiérrez-Escribano, Pilar / Arnáiz-Pita, Yolanda / Dueñas-Santero, Encarnación / Belén Suárez, M. / Bougnoux, Marie-Elisabeth / del Rey, Francisco / Sherlock, Gavin / D'Enfert, Christophe / Correa-Bordes, Jaime / Vázquez de Aldana, Carlos R.

    Plos Genetics 4 (11), . (2015)

    Abstract: Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for ... ...

    Abstract Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for filamentous growth is the ability to inhibit cell separation after cytokinesis, although it is poorly understood how this process is regulated developmentally. In C. albicans, the formation of filaments during hyphal growth requires changes in septin ring dynamics. In this work, we studied the functional relationship between septins and the transcription factor Ace2, which controls the expression of enzymes that catalyze septum degradation. We found that alternative translation initiation produces two Ace2 isoforms. While full-length Ace2, Ace2L, influences septin dynamics in a transcription-independent manner in hyphal cells but not in yeast cells, the use of methionine-55 as the initiation codon gives rise to Ace2S, which functions as the nuclear transcription factor required for the expression of cell separation genes. Genetic evidence indicates that Ace2L influences the incorporation of the Sep7 septin to hyphal septin rings in order to avoid inappropriate activation of cell separation during filamentous growth. Interestingly, a natural single nucleotide polymorphism (SNP) present in the C. albicans WO-1 background and other C. albicans commensal and clinical isolates generates a stop codon in the ninth codon of Ace2L that mimics the phenotype of cells lacking Ace2L. Finally, we report that Ace2L and Ace2S interact with the NDR kinase Cbk1 and that impairing activity of this kinase results in a defect in septin dynamics similar to that of hyphal cells lacking Ace2L. Together, our findings identify Ace2L and the NDR kinase Cbk1 as new elements of the signaling system that modify septin ring dynamics in hyphae to allow cell-chain formation, a feature that appears to have evolved in specific C. albicans lineages.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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