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  1. Article: A species-specific lncRNA modulates the reproductive ability of the asian tiger mosquito.

    Belavilas-Trovas, Alexandros / Gregoriou, Maria-Eleni / Tastsoglou, Spyros / Soukia, Olga / Giakountis, Antonis / Mathiopoulos, Kostas

    Frontiers in bioengineering and biotechnology

    2022  Volume 10, Page(s) 885767

    Abstract: Long non-coding RNA (lncRNA) research has emerged as an independent scientific field in recent years. Despite their association with critical cellular and metabolic processes in plenty of organisms, lncRNAs are still a largely unexplored area in mosquito ...

    Abstract Long non-coding RNA (lncRNA) research has emerged as an independent scientific field in recent years. Despite their association with critical cellular and metabolic processes in plenty of organisms, lncRNAs are still a largely unexplored area in mosquito research. We propose that they could serve as exceptional tools for pest management due to unique features they possess. These include low inter-species sequence conservation and high tissue specificity. In the present study, we investigated the role of ovary-specific lncRNAs in the reproductive ability of the Asian tiger mosquito,
    Language English
    Publishing date 2022-08-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2022.885767
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction.

    Belavilas-Trovas, Alexandros / Tastsoglou, Spyros / Dong, Shengzhang / Kefi, Mary / Tavadia, Mihra / Mathiopoulos, Kostas D / Dimopoulos, George

    PLoS pathogens

    2023  Volume 19, Issue 6, Page(s) e1011440

    Abstract: Long non-coding RNAs (lncRNAs) play critical regulatory roles in various cellular and metabolic processes in mosquitoes and all other organisms studied thus far. In particular, their involvement in essential processes such as reproduction makes them ... ...

    Abstract Long non-coding RNAs (lncRNAs) play critical regulatory roles in various cellular and metabolic processes in mosquitoes and all other organisms studied thus far. In particular, their involvement in essential processes such as reproduction makes them potential targets for the development of novel pest control approaches. However, their function in mosquito biology remains largely unexplored. To elucidate the role of lncRNAs in mosquitoes' reproduction and vector competence for arboviruses, we have implemented a computational and experimental pipeline to mine, screen, and characterize lncRNAs related to these two biological processes. Through analysis of publicly available Zika virus (ZIKV) infection-regulated Aedes aegypti transcriptomes, at least six lncRNAs were identified as being significantly upregulated in response to infection in various mosquito tissues. The roles of these ZIKV-regulated lncRNAs (designated Zinc1, Zinc2, Zinc3, Zinc9, Zinc10 and Zinc22), were further investigated by dsRNA-mediated silencing studies. Our results show that silencing of Zinc1, Zinc2, and Zinc22 renders mosquitoes significantly less permissive to ZIKV infection, while silencing of Zinc22 also reduces fecundity, indicating a potential role for Zinc22 in trade-offs between vector competence and reproduction. We also found that silencing of Zinc9 significantly increases fecundity but has no effect on ZIKV infection, suggesting that Zinc9 may be a negative regulator of oviposition. Our work demonstrates that some lncRNAs play host factor roles by facilitating viral infection in mosquitoes. We also show that lncRNAs can influence both mosquito reproduction and permissiveness to virus infection, two biological systems with important roles in mosquito vectorial capacity.
    MeSH term(s) Animals ; Female ; Zika Virus/physiology ; Zika Virus Infection ; Aedes/genetics ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Mosquito Vectors/genetics ; Reproduction
    Chemical Substances RNA, Long Noncoding
    Language English
    Publishing date 2023-06-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1011440
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Anosmic flies: what Orco silencing does to olive fruit flies.

    Tsoumani, Konstantina T / Belavilas-Trovas, Alexandros / Gregoriou, Maria-Eleni / Mathiopoulos, Kostas D

    BMC genetics

    2020  Volume 21, Issue Suppl 2, Page(s) 140

    Abstract: Background: The olive fruit fly (Bactrocera oleae) is the most destructive pest of the olive cultivation worldwide causing significant production losses and olive fruit impoverishment, as its larvae feed exclusively on the olive fruit. Reproductive and ... ...

    Abstract Background: The olive fruit fly (Bactrocera oleae) is the most destructive pest of the olive cultivation worldwide causing significant production losses and olive fruit impoverishment, as its larvae feed exclusively on the olive fruit. Reproductive and sexual behavior, as well as host-plant recognition of the fly, are highly dependent on its chemosensory system. Therefore, exploring the role of genes that play a critical role in olfaction, could reveal potential molecular targets that determine species-specific features on chemical communication and could be used to impair sexual behavior.
    Results: In this study we identified the gene that encodes the conserved olfactory co-receptor Orco (Odorant receptor co-receptor), which interacts with all divergent insect odorant receptors, and investigated how disruption of its expression affects chemoreception. We initially searched the expression profile of Bo-Orco in both sexes during sexual maturation, as well as pre- and post-mating communication by relative quantitative real time PCR (qRT-PCR) analysis suggesting that Bo-Orco was abundantly expressed in sexually mature adults. We further investigated the functional role of Bo-Orco in mating and oviposition behavior via transient gene silencing that was performed through in vivo dsRNA hemolymph injections in sexually mature flies 7 days after eclosion. Orco-knockdown phenotypes in both sexes showed reduced copulation rates in mating competitiveness tests, possibly through impaired olfactory-mediated detection of sex pheromone. In addition, oviposition was significantly inhibited in dsRNA-Orco injected females in a post-mating behavior test.
    Conclusions: Our results demonstrate that Orco plays a crucial role in the reproductive behavior of the olive fruit fly, since pre- and post-mating processes were affected. This is the first report in the olive fruit fly that links the chemosensory pathway with the mating behavior and the reproductive potential at a molecular basis, rendering this gene a potential target for the improvement of the olive fruit fly population control techniques.
    MeSH term(s) Amino Acid Sequence ; Animals ; Female ; Gene Silencing ; Insect Control ; Insect Proteins/genetics ; Male ; Olea ; Oviposition ; Receptors, Odorant/genetics ; Sexual Behavior, Animal ; Tephritidae/genetics ; Tephritidae/physiology
    Chemical Substances Insect Proteins ; Receptors, Odorant
    Language English
    Publishing date 2020-12-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1471-2156
    ISSN (online) 1471-2156
    DOI 10.1186/s12863-020-00937-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Development of DNA Aptamers Against

    Lantero, Elena / Belavilas-Trovas, Alexandros / Biosca, Arnau / Recolons, Paula / Moles, Ernest / Sulleiro, Elena / Zarzuela, Francesc / Ávalos-Padilla, Yunuen / Ramírez, Miriam / Fernàndez-Busquets, Xavier

    Journal of biomedical nanotechnology

    2020  Volume 16, Issue 3, Page(s) 315–334

    Abstract: New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target ... ...

    Abstract New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is
    MeSH term(s) Animals ; Aptamers, Nucleotide ; Diagnostic Tests, Routine ; Humans ; Malaria, Vivax ; Plasmodium falciparum ; SELEX Aptamer Technique
    Chemical Substances Aptamers, Nucleotide
    Language English
    Publishing date 2020-06-03
    Publishing country United States
    Document type Journal Article
    ISSN 1550-7033
    ISSN 1550-7033
    DOI 10.1166/jbn.2020.2901
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Inter-library loan at ZB MED

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