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  1. Article ; Online: Spatiotemporal water quality variability in a highly loaded surface flow wastewater treatment wetland.

    Hitchcock, Daniel R / Bell, Natasha L / Strosnider, William / Smith, Matt C

    Journal of environmental quality

    2022  Volume 51, Issue 1, Page(s) 101–111

    Abstract: This study evaluates spatiotemporal relationships between water quality parameters (WQPs), nutrients, suspended solids, and biochemical oxygen demand (BOD) concentrations within an engineered wastewater treatment wetland system in the Georgia Piedmont, ... ...

    Abstract This study evaluates spatiotemporal relationships between water quality parameters (WQPs), nutrients, suspended solids, and biochemical oxygen demand (BOD) concentrations within an engineered wastewater treatment wetland system in the Georgia Piedmont, USA. We explored factors related to treatment efficiency within a heavily loaded 630-m
    MeSH term(s) Nitrogen/analysis ; Sewage ; Waste Disposal, Fluid ; Waste Water ; Water Purification ; Water Quality ; Wetlands
    Chemical Substances Sewage ; Waste Water ; Nitrogen (N762921K75)
    Language English
    Publishing date 2022-01-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 120525-0
    ISSN 1537-2537 ; 0047-2425
    ISSN (online) 1537-2537
    ISSN 0047-2425
    DOI 10.1002/jeq2.20309
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Spatiotemporal water quality variability in a highly loaded surface flow wastewater treatment wetland

    Hitchcock, Daniel R. / Bell, Natasha L. / Strosnider, William / Smith, Matt C.

    Journal of environmental quality. 2022 Jan., v. 51, no. 1

    2022  

    Abstract: This study evaluates spatiotemporal relationships between water quality parameters (WQPs), nutrients, suspended solids, and biochemical oxygen demand (BOD) concentrations within an engineered wastewater treatment wetland system in the Georgia Piedmont, ... ...

    Abstract This study evaluates spatiotemporal relationships between water quality parameters (WQPs), nutrients, suspended solids, and biochemical oxygen demand (BOD) concentrations within an engineered wastewater treatment wetland system in the Georgia Piedmont, USA. We explored factors related to treatment efficiency within a heavily loaded 630‐m² surface flow wetland system over a 2‐yr period. Relationships between temperature, dissolved oxygen (DO), and oxidation‐reduction potential (ORP) were observed; relationships were also seen between these WQPs and nutrient concentrations. Because temperature, DO, and ORP affect nitrogen (N) cycling rates, seasonal trends in N forms were evident in the system. Organic N and inorganic/organic phosphorus concentrations correlated with solids concentrations in the vegetated system without exhibiting seasonal trends. Surface water within the vegetated section generally exhibited anoxic conditions, leading to removal of nitrate‐N within the system; however, limited mineralization and nitrification occurred, which greatly limited overall N removal. Plant selection and lack of maintenance likely led to high solids and BOD contributions to treatment wetland surface water, which varied substantially between and along monitored transects. Because so few studies have investigated treatment dynamics within treatment wetland cells, focusing solely on influent/effluent characterization, radical spatiotemporal variability may be the norm as opposed to the commonly accepted assumptions of relatively uniform pollutant degradation across treatment wetland cells. This spatiotemporal variability in WQPs underscores the dynamic nature of treatment wetlands and the need for routine maintenance, including sludge removal and plant harvesting.
    Keywords biochemical oxygen demand ; mineralization ; nitrate nitrogen ; nitrification ; nitrogen ; organic phosphorus ; overland flow ; oxygen ; piedmont ; pollutants ; redox potential ; sludge ; surface water ; temperature ; wastewater treatment ; water quality ; wetlands ; Georgia
    Language English
    Dates of publication 2022-01
    Size p. 101-111.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 120525-0
    ISSN 1537-2537 ; 0047-2425
    ISSN (online) 1537-2537
    ISSN 0047-2425
    DOI 10.1002/jeq2.20309
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Potential Susceptibility of Six Aquatic Plant Species to Infection by Five Species of

    Bell, Natasha L / Jeffers, Steven N / Hitchcock, Daniel R / White, Sarah A

    Plant disease

    2021  Volume 105, Issue 12, Page(s) 4074–4083

    Abstract: Investigations of the susceptibility of aquatic plants to species ... ...

    Abstract Investigations of the susceptibility of aquatic plants to species of
    MeSH term(s) Phytophthora ; Plant Leaves ; Plant Roots ; Plants ; Rhododendron
    Language English
    Publishing date 2021-12-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-10-20-2190-RE
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Effects of monensin and protein supplementation on intake, digestion, and ruminal fermentation in beef cattle consuming low-quality forage

    Martinez, Javier J. / Löest, Clint A. / McCuistion, Kimberly C. / Wester, David B. / Bell, Natasha L.

    Applied animal science. 2022 Feb., v. 38, no. 1

    2022  

    Abstract: The objective of this study was to evaluate the effects of monensin and protein supplementation and their interaction on intake, apparent digestion, and ruminal fermentation variables in cattle consuming low-quality forage. Four ruminally cannulated cows ...

    Abstract The objective of this study was to evaluate the effects of monensin and protein supplementation and their interaction on intake, apparent digestion, and ruminal fermentation variables in cattle consuming low-quality forage. Four ruminally cannulated cows (637 ± 24 kg of BW) were used in a 4 × 4 Latin square design. Treatments were arranged as a 2 × 2 factorial: (1) monensin (0 or 200 mg∙cow⁻¹∙d⁻¹) and (2) protein (0 or 0.64 kg∙cow⁻¹∙d⁻¹ CP). Day 1 through 4 of each period, animals were fed only low-quality forage, d 5 through 14 allowed for treatment adaptation, and d 15 through 20 were for sample collection. Data were analyzed using the MIXED procedure of SAS 9.4 (SAS Institute Inc.). Neither a monensin × protein interaction nor a monensin effect (P ≥ 0.30) was observed for any intake or digestion variable measured. In contrast, protein treatment increased (P < 0.01) all measures of intake. Protein increased (P < 0.01) OM digestibility, total digestible OM intake, and total digestible NDF intake but had no effect (P = 0.13) on NDF digestibility. A monensin × protein interaction (P = 0.33) or monensin effect (P = 0.34) were not observed for total VFA concentration, but protein increased (P < 0.01) total VFA concentration. A tendency for monensin × protein interaction was observed for the acetate:propionate ratio (P = 0.06) and molar percentage of propionate. Monensin increased (P < 0.01) molar percentage of propionate but had no effect (P = 0.21) on acetate. Although monensin altered ruminal VFA profiles, providing monensin to cows consuming a low-quality-forage diet provided no benefits in forage intake or digestion.
    Keywords acetates ; beef cattle ; cannulas ; digestibility ; feed intake ; forage ; monensin ; propionic acid ; protein supplements ; rumen fermentation
    Language English
    Dates of publication 2022-02
    Size p. 13-21.
    Publishing place Elsevier Inc.
    Document type Article
    ISSN 2590-2865
    DOI 10.15232/aas.2021-02219
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Potential Susceptibility of Six Aquatic Plant Species to Infection by Five Species of Phytophthora

    Bell, Natasha L. / Jeffers, Steven N. / Hitchcock, Daniel R. / White, Sarah A.

    Plant disease. 2021 Dec. 30, v. 105, no. 12

    2021  

    Abstract: Investigations of the susceptibility of aquatic plants to species of Phytophthora are limited. Therefore, the objective of this study was to assess the potential susceptibility of six aquatic plant species, frequently used in constructed wetlands or ... ...

    Abstract Investigations of the susceptibility of aquatic plants to species of Phytophthora are limited. Therefore, the objective of this study was to assess the potential susceptibility of six aquatic plant species, frequently used in constructed wetlands or vegetated channels, to infection by five species of Phytophthora commonly found at nurseries in the southeastern United States. In a greenhouse experiment, roots of each plant species (Agrostis alba, Carex stricta, Iris ensata ‘Rising Sun’, Panicum virgatum, Pontederia cordata, and Typha latifolia) growing in aqueous solutions were exposed to zoospores of each of the species of Phytophthora (Phytophthora cinnamomi, Phytophthora citrophthora, Phytophthora cryptogea, Phytophthora nicotianae, and Phytophthora palmivora). Zoospore presence and activity in solution were monitored with a standard baiting bioassay with rhododendron leaf discs as baits. Experiments were initiated in 2016 and repeated in 2017 and 2018. During the 2016 trials, Phytophthora spp. were not isolated from the roots of any of the plants, but some roots of C. stricta, P. virgatum, and T. latifolia were infected with multiple species of Phytophthora during trials in 2017 and 2018. Presence of plant roots reduced the percentage of rhododendron leaf discs infected by zoospores of four of the species of Phytophthora but not those infected by P. cinnamomi, which suggested that roots of these plants negatively affected the presence or activity of zoospores of these four species of Phytophthora in the aqueous growing solution. Results from this study demonstrated that certain aquatic plant species may be sources of inoculum at ornamental plant nurseries if these plants are present naturally in waterways or used in constructed wetlands treating water flowing off production areas, which could be of concern to plant producers who recycle irrigation water.
    Keywords Agrostis ; Carex stricta ; Iris ensata ; Panicum virgatum ; Phytophthora cinnamomi ; Phytophthora citrophthora ; Phytophthora cryptogea ; Phytophthora nicotianae ; Phytophthora palmivora ; Pontederia cordata ; Rhododendron ; Typha latifolia ; aquatic plants ; bioassays ; greenhouse experimentation ; inoculum ; irrigation water ; leaves ; ornamental plants ; zoospores
    Language English
    Dates of publication 2021-1230
    Size p. 4074-4083.
    Publishing place The American Phytopathological Society
    Document type Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-10-20-2190-RE
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Effects of cyclic monensin feeding on ruminal function in cannulated beef steers consuming low-quality forage

    Hook, Kayla C. / Machen, Richard V. / Wester, David B. / McCuistion, Kimberly C. / Gonzalez, Daisy A. / Hernandez, Alicia G. / Bell, Natasha L.

    Applied animal science. 2022 Apr., v. 38, no. 2

    2022  

    Abstract: The aim of this research was to evaluate the effects of cyclically feeding monensin (Rumensin 90, Elanco Animal Health) to ruminally cannulated steers (12 Bos taurus; 260 kg of BW) consuming low-quality forage (4.9% CP) on measures of intake, digestion, ... ...

    Abstract The aim of this research was to evaluate the effects of cyclically feeding monensin (Rumensin 90, Elanco Animal Health) to ruminally cannulated steers (12 Bos taurus; 260 kg of BW) consuming low-quality forage (4.9% CP) on measures of intake, digestion, and ruminal function. Twelve steers were randomly assigned to 1 of 3 treatments in a completely randomized design: (1) no monensin (CON); (2) 200 mg·head⁻¹·d⁻¹ monensin (MON); or (3) 200 mg·head⁻¹·d⁻¹ monensin for 14 d and 0 mg·head⁻¹·d⁻¹ monensin for the subsequent 14 d (CYC; recurrent cycle). Hay and ort samples were collected d 10 through 13 for intake determination during each of the four 28-d replicated cycles. Rumen fluid was collected with a suction strainer 0, 2, 4, 8, and 12 h after feeding on d 14 for pH and VFA analysis. Data were analyzed using the MIXED procedure of SAS 9.4 (SAS Institute Inc.). Treatment × cycle interactions or treatment effects were not observed for any measure of intake (OM intake, NDF intake; P ≥ 0.17) or pH (P = 0.13). A tendency for a treatment × cycle interaction was observed (P = 0.08) for OM digestion but not for NDF digestion (P = 0.37). Treatment × cycle interactions were observed (P < 0.01) for molar proportions of acetate, propionate, and acetate:propionate ratio. Because these treatment × cycle interactions occurred, treatment × hour interactions, treatment effects, and hour effects were evaluated within cycles for these parameters. Treatment × hour interactions (P ≤ 0.02) occurred for molar proportion of propionate in cycle 1 and propionate and acetate:propionate ratio in cycle 3, and there was a tendency (P = 0.09) for a treatment × hour interaction for acetate:propionate in cycle 4. Although treatment effects were observed for molar proportions of acetate, propionate, and acetate:propionate ratio in cycle 1 (P ≤ 0.01), these effects had diminished by cycle 4 (P ≥ 0.39). An effect of hour after feeding was observed (P ≤ 0.02) in all 4 cycles for acetate, propionate, acetate:propionate, and ruminal pH. Further evaluation of monensin feeding methods that vary duration of feeding and withdrawal periods may be warranted. Additionally, further study concerning differences in the effect of these methods on a diet bases on concentrate versus low-quality forage warrant investigation.
    Keywords acetates ; beef ; cannulas ; diet ; forage ; monensin ; pH ; propionic acid ; rumen fermentation ; rumen fluids
    Language English
    Dates of publication 2022-04
    Size p. 129-140.
    Publishing place Elsevier Inc.
    Document type Article
    ISSN 2590-2865
    DOI 10.15232/aas.2021-02202
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Phytophthora Species Associated with Plants in Constructed Wetlands and Vegetated Channels at a Commercial Plant Nursery Over Time

    Ridge, Garrett A / Bell, Natasha L / Gitto, Andrew J / Jeffers, Steven N / White, Sarah A

    HortTechnology. 2019 Dec., v. 29, no. 6

    2019  

    Abstract: Constructed wetlands have been used for decades in agricultural settings to remediate nutrients and other agrichemicals from irrigation runoff and drainage; however, little is known about the presence and distribution of Phytophthora species within ... ...

    Abstract Constructed wetlands have been used for decades in agricultural settings to remediate nutrients and other agrichemicals from irrigation runoff and drainage; however, little is known about the presence and distribution of Phytophthora species within irrigation runoff water being treated in constructed wetlands. Therefore, we collected plant samples from within vegetated runoff collection channels and treatment stages of two constructed wetland systems receiving irrigation runoff at a commercial plant nursery in Cairo, GA, to determine if roots of wetland plants were infested by species of Phytophthora. Samples were collected 12 times, at 1- to 2-month intervals, over a 19-month period, from Mar. 2011 through Sept. 2012. The sample period covered all four seasons of the year, so we could determine if the association of Phytophthora species with roots of specific plant species varied with season. Approximately 340 samples from 14 wetland plant species were collected, and 22 isolates of Phytophthora species were recovered. Phytophthora species were typically isolated from plants in channels receiving runoff water directly from plant production areas; Phytophthora species were not detected on plants where water leaves the nursery. No seasonal patterns were observed in plant infestation or presence of species of Phytophthora. In fact, Phytophthora species were rarely found to be associated with the roots of the wetland plants collected; species of Phytophthora were found infesting roots of only 6.5% of the 336 plants sampled. Species of Phytophthora were not found to be associated with the roots of golden canna (Canna flaccida), lamp rush (Juncus effusus var. solutus), duckweed (Lemna valdiviana), or sedges (Carex sp.) during the study period. The exotic invasive plant species marsh dayflower [Murdannia keisak (33% of samples infested)] and alligatorweed [Alternanthera philoxeroides (15% of samples infested)] were found to have the first and third highest, respectively, incidences of infestation, with smooth beggartick (Bidens laevis) having the second highest incidence of samples infested (22%). Management of invasive species in drainage canals and constructed wetland systems may be critical because of their potential propensity toward infestation by Phytophthora species. Plant species recommended for further investigation for use in constructed wetlands to remediate irrigation runoff include golden canna, marsh pennywort (Hydrocotyle umbellata), pickerelweed (Pontederia cordata), and broadleaf cattail (Typha latifolia). The results from this study provide an important first look at the associations between species of Phytophthora and wetland plants in constructed wetland systems treating irrigation runoff and will serve to further optimize the design of constructed wetlands and other vegetation-based treatment technologies for the removal of plant pathogens from irrigation runoff.
    Keywords Alternanthera philoxeroides ; Bidens ; Canna ; Carex ; Hydrocotyle ; Juncus effusus ; Lemna ; Phytophthora ; Pontederia cordata ; Typha latifolia ; constructed wetlands ; drainage ; invasive species ; irrigation ; marshes ; plant nurseries ; runoff ; wetland plants
    Language English
    Dates of publication 2019-12
    Size p. 736-744.
    Publishing place American Society for Horticultural Science
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1115665-x
    ISSN 1063-0198
    ISSN 1063-0198
    DOI 10.21273/HORTTECH04300-19
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Protocol and Interactive Routine forthe Design of Subsurface Bioreactors

    Cooke, Richard A / Bell, Natasha L

    Applied engineering in agriculture. 2014 Sept., v. 30, no. 5

    2014  

    Abstract: One potential practice to reduce nitrate and phosphate transfer from subsurface (tile)-drained farmlands is the use of fixed-bed, in-field subsurface bioreactors. These bioreactors are trenches filled with carbon material (usually wood chips), which ... ...

    Abstract One potential practice to reduce nitrate and phosphate transfer from subsurface (tile)-drained farmlands is the use of fixed-bed, in-field subsurface bioreactors. These bioreactors are trenches filled with carbon material (usually wood chips), which serve as a medium for denitrifying bacteria to grow, thus reducing the amount of nitrate that enters water bodies from tile drains. If a bioreactor is undersized, water will not be detained long enough for denitrification to occur, or much of the water will bypass the system. On the other hand, if it is oversized, extreme reducing conditions can result in the formation of deleterious compounds. In this article, we propose a new protocol for optimizing the size of a bioreactor, describe an interactive routine in which this protocol has been implemented, and present the application of the routine to the design of a bioreactor in southern Minnesota. The routine is available in the online version of the Illinois Drainage Guide.
    Keywords bioreactors ; carbon ; denitrification ; denitrifying bacteria ; drainage ; engineering ; nitrates ; surface water ; wood chips ; Illinois ; Minnesota
    Language English
    Dates of publication 2014-09
    Size p. 761-771.
    Publishing place American Society of Agricultural Engineers
    Document type Article
    ZDB-ID 54345-7
    ISSN 0883-8542
    ISSN 0883-8542
    DOI 10.13031/aea.30.9900
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  9. Article: Effect of monensin inclusion on intake, digestion, and ruminal fermentation parameters by Bos taurus indicus and Bos taurus taurus steers consuming bermudagrass hay

    Bell, Natasha L / Anderson, Robin C / Callaway, Todd R / Franco, Marcia O / Sawyer, Jason E / Wickersham, Tryon A

    Journal of animal science. 2017 June 8, v. 95, no. 6

    2017  

    Abstract: Effects of monensin inclusion and cattle subspecies on utilization of bermudagrass hay (13.7% CP, 77.3% NDF, and 38.8% ADF) were evaluated using ruminally cannulated steers (5 Bos taurus indicus [BI] and 5 Bos taurus taurus [BT]; 398 kg BW). Subspecies ... ...

    Abstract Effects of monensin inclusion and cattle subspecies on utilization of bermudagrass hay (13.7% CP, 77.3% NDF, and 38.8% ADF) were evaluated using ruminally cannulated steers (5 Bos taurus indicus [BI] and 5 Bos taurus taurus [BT]; 398 kg BW). Subspecies were concurrently subjected to a 2-period, 2-treatment crossover design. Treatments were 0 (CON) or 200 mg·steer**-1·d**-1 monensin (MON) in 0.91 kg dried distillers’ grains with solubles. Periods were 70 d in length: 20 d of adaptation, 22 d of sample collection, and 28 d for withdrawal of treatment. Steers were group housed during adaptation and moved to individual covered pens for sampling. Hay, ort, and fecal grab samples were collected d 21 through 25 for determination of intake and digestion. Ruminal fluid was collected with a suction strainer 0, 2, 4, 8, and 12 h after feeding on d 42 for pH, VFA, and ruminal NH3–N (RAN) analysis. Additionally, at h 2, ruminal fluid and contents were collected for determination of rate of NH3 production and CH4 production rate. No subspecies × monensin interactions were observed (P greater than or equal to 0.12). Monensin had no effect (P greater than or equal to 0.16) on intake or digestibility parameters. No subspecies effect (P greater than or equal to 0.11) was observed for forage OM intake, total OM intake, or OM digestion. Total digestible OM intake tended to be greater (P = 0.06) for BT steers than for BI steers (14.0 vs. 12.2 g/kg BW). There was an effect of hour after feeding (P less than or equal to 0.01) on pH, total VFA, acetate:propionate ratio, and molar percent acetate and propionate. Total VFA concentration was greater (P = 0.01) in CON steers than in MON steers (66.5 vs. 62.0 mM). Monensin decreased molar percent acetate (P = 0.02) from 72.5 to 71.2% and increased molar percent propionate (P < 0.01) from 16.9 to 18.7%, resulting in a reduced (P < 0.01) acetate:propionate ratio (from 4.34 to 3.85). Although not significantly (P = 0.19), monensin numerically reduced the CH4 production rate by 15.8%. Greater (P = 0.07) CH4 production rate tended to be observed in BI steers than in BT steers (21.4 vs. 16.6 μmol CH4·mL**-1·h**-1, respectively). Monensin had no effect (P greater than or equal to 0.32) on pH, RAN, or rate of NH3 production. A subspecies × hour after feeding interaction was observed for RAN, with BT having greater RAN at h 0 and 4, whereas BI had greater RAN at h 2, 8, and 12. Overall, monensin decreased the acetate:propionate ratio and total VFA concentration but had no effect on forage utilization. Bos taurus indicus steers consumed less digestible OM and had a greater CH4 production rate compared with BT steers, suggesting BT were better able to utilize the available forage resource than BI.
    Keywords Cynodon dactylon ; acetates ; ammonia ; ammonium nitrogen ; cannulas ; cross-over studies ; digestibility ; feed conversion ; forage ; hay ; methane ; methane production ; monensin ; pH ; propionic acid ; rumen fermentation ; rumen fluids ; steers
    Language English
    Dates of publication 2017-0608
    Size p. 2736-2746.
    Document type Article
    ZDB-ID 390959-1
    ISSN 1525-3163 ; 0021-8812
    ISSN (online) 1525-3163
    ISSN 0021-8812
    DOI 10.2527/jas2016.1011
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  10. Article: Effect of monensin withdrawal on intake, digestion, and ruminal fermentation parameters by Bos taurus indicus and Bos taurus taurus steers consuming bermudagrass hay

    Bell, Natasha L / Callaway, Todd R / Anderson, Robin C / Franco, Marcia O / Sawyer, Jason E / Wickersham, Tyron A

    Journal of animal science. 2017 June 1, v. 95, no. 6

    2017  

    Abstract: Effects of monensin inclusion and cattle subspecies on utilization of bermudagrass hay (13.7% CP, 77.3% NDF, and 38.8% ADF) were evaluated using ruminally cannulated steers (5 Bos taurus indicus [BI] and 5 Bos taurus taurus [BT]; 398 kg BW). ... ...

    Abstract Effects of monensin inclusion and cattle subspecies on utilization of bermudagrass hay (13.7% CP, 77.3% NDF, and 38.8% ADF) were evaluated using ruminally cannulated steers (5 Bos taurus indicus [BI] and 5 Bos taurus taurus [BT]; 398 kg BW). Subspecies were concurrently subjected to a 2-period, 2-treatment crossover design. Treatments were 0 (CON) or 200 mg·steer-1·d-1 monensin (MON) in 0.91 kg dried distillers’ grains with solubles. Periods were 70 d in length: 20 d of adaptation, 22 d of sample collection, and 28 d for withdrawal of treatment. Steers were group housed during adaptation and moved to individual covered pens for sampling. Hay, ort, and fecal grab samples were collected d 21 through 25 for determination of intake and digestion. Ruminal fluid was collected with a suction strainer 0, 2, 4, 8, and 12 h after feeding on d 42 for pH, VFA, and ruminal NH3–N (RAN) analysis. Additionally, at h 2, ruminal fluid and contents were collected for determination of rate of NH3 production and CH4 production rate. No subspecies × monensin interactions were observed (P = 0.12). Monensin had no effect (P = 0.16) on intake or digestibility parameters. No subspecies effect (P = 0.11) was observed for forage OM intake, total OM intake, or OM digestion. Total digestible OM intake tended to be greater (P = 0.06) for BT steers than for BI steers (14.0 vs. 12.2 g/kg BW). There was an effect of hour after feeding (P = 0.01) on pH, total VFA, acetate:propionate ratio, and molar percent acetate and propionate. Total VFA concentration was greater (P = 0.01) in CON steers than in MON steers (66.5 vs. 62.0 mM). Monensin decreased molar percent acetate (P = 0.02) from 72.5 to 71.2% and increased molar percent propionate (P < 0.01) from 16.9 to 18.7%, resulting in a reduced (P < 0.01) acetate:propionate ratio (from 4.34 to 3.85). Although not significantly (P = 0.19), monensin numerically reduced the CH4 production rate by 15.8%. Greater (P = 0.07) CH4 production rate tended to be observed in BI steers than in BT steers (21.4 vs. 16.6 µmol CH4·mL-1·h-1, respectively). Monensin had no effect (P = 0.32) on pH, RAN, or rate of NH3 production. A subspecies × hour after feeding interaction was observed for RAN, with BT having greater RAN at h 0 and 4, whereas BI had greater RAN at h 2, 8, and 12. Overall, monensin decreased the acetate:propionate ratio and total VFA concentration but had no effect on forage utilization. Bos taurus indicus steers consumed less digestible OM and had a greater CH4 production rate compared with BT steers, suggesting BT were better able to utilize the available forage resource than BI.
    Keywords Cynodon dactylon ; acetates ; ammonia ; ammonium nitrogen ; cannulas ; cross-over studies ; digestibility ; distillers grains ; forage ; group housing ; hay ; methane ; methane production ; monensin ; pH ; propionic acid ; rumen fermentation ; rumen fluids ; steers
    Language English
    Dates of publication 2017-0601
    Size p. 2737-2746.
    Document type Article
    ZDB-ID 390959-1
    ISSN 1525-3163 ; 0021-8812
    ISSN (online) 1525-3163
    ISSN 0021-8812
    DOI 10.2527/jas2016.1013
    Database NAL-Catalogue (AGRICOLA)

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