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Article ; Online: Balancing gene transfection and cytotoxicity of nucleic acid carriers with focus on ocular and hepatic disorders: evaluation of hydrophobic and hydrophilic polyethyleneimine derivatives.

de Oliveira, Fernando A / Albuquerque, Lindomar J C / Nascimento-Sales, Michelle / Christoffolete, Marcelo A / Bellettini, Ismael C / Giacomelli, Fernando C

Journal of materials chemistry. B

2023 Volume 11, Issue 20, Page(s) 4556–4571

Abstract: Polyethyleneimine (PEI) derivatives substituted by lactose, succinic acid or alkyl domains were evaluated as nonviral gene delivery vectors towards balancing gene transfection and cytotoxicity. The investigations were focused on pDNA transfection into ... ...

Abstract	Polyethyleneimine (PEI) derivatives substituted by lactose, succinic acid or alkyl domains were evaluated as nonviral gene delivery vectors towards balancing gene transfection and cytotoxicity. The investigations were focused on pDNA transfection into arising retinal pigment epithelia (ARPE-19) and human hepatocellular carcinoma (HepG2) cell lines. The first mentioned cell line was chosen as motivated by the non-negligible number of ocular disorders linked to gene aberrations, whereas the second one is a cell line overexpressing the asialoglycoprotein receptor (ASGP-R), which can bind to galactose residues. The presence of short alkyl domains (C
MeSH term(s)	Humans ; Polyethyleneimine/chemistry ; Plasmids ; Nucleic Acids ; Succinic Acid ; Lactose ; Transfection ; DNA/genetics ; DNA/chemistry ; Liver Neoplasms/genetics
Chemical Substances	Polyethyleneimine (9002-98-6) ; Nucleic Acids ; Succinic Acid (AB6MNQ6J6L) ; Lactose (J2B2A4N98G) ; DNA (9007-49-2)
Language	English
Publishing date	2023-05-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d3tb00477e
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Polyethylenimine as a Versatile Simultaneous Reducing and Stabilizing Agent Enabling One-Pot Synthesis of Transition-Metal Nanoparticles: Fundamental Aspects and Practical Implications.

Ribeiro, Caroline A S / Panico, Karine / Handajevsky, Tamara J / da Silva, Fernanda Dias / Bellettini, Ismael C / Pavlova, Ewa / Giacomelli, Fernando C

Langmuir : the ACS journal of surfaces and colloids

2023 Volume 39, Issue 48, Page(s) 17353–17365

Abstract: The large surface area of metallic nanoparticles provides them with particular optical, chemical, and biological properties, accordingly enabling their use in a wide array of applications. In this regard, facile and fast synthetic approaches are ... ...

Abstract	The large surface area of metallic nanoparticles provides them with particular optical, chemical, and biological properties, accordingly enabling their use in a wide array of applications. In this regard, facile and fast synthetic approaches are desirable for ready-to-use functional materials. Following early investigations focused on the direct synthesis of polymer-coated gold nanoparticles, we herein demonstrate that such a strategy can be used to manufacture different types of d-block transition-metal nanoparticles via a one-pot method in aqueous media and mild temperature conditions. Gold (Au
Language	English
Publishing date	2023-11-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2005937-1
ISSN	1520-5827 ; 0743-7463
ISSN (online)	1520-5827
ISSN	0743-7463
DOI	10.1021/acs.langmuir.3c02538
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Article ; Online: Reduced cytotoxicity of nanomaterials driven by nano-bio interactions: Case study of single protein coronas enveloping polymersomes.

de Oliveira, Fernando A / Albuquerque, Lindomar J C / Castro, Carlos E / Riske, Karin A / Bellettini, Ismael C / Giacomelli, Fernando C

Colloids and surfaces. B, Biointerfaces

2022 Volume 213, Page(s) 112387

Abstract: The protein adsorption onto poly(acrylic acid)-block-polystyrene ( ... ...

Abstract	The protein adsorption onto poly(acrylic acid)-block-polystyrene (PAA
MeSH term(s)	Adsorption ; Nanoparticles/chemistry ; Nanostructures ; Protein Corona/chemistry ; Serum Albumin, Bovine/chemistry
Chemical Substances	Protein Corona ; Serum Albumin, Bovine (27432CM55Q)
Language	English
Publishing date	2022-02-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2022.112387
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Article: Reduced cytotoxicity of nanomaterials driven by nano-bio interactions: Case study of single protein coronas enveloping polymersomes

de Oliveira, Fernando A. / Albuquerque, Lindomar J.C. / Castro, Carlos E. / Riske, Karin A. / Bellettini, Ismael C. / Giacomelli, Fernando C.

Colloids and surfaces. 2022 May, v. 213

2022

Abstract: The protein adsorption onto poly(acrylic acid)-block-polystyrene (PAA₂₂-b-PS₁₄₄) polymersomes has been investigated with regard to structural features, thermodynamic aspects and biological consequences. The light scattering measurements revealed the ... ...

Abstract	The protein adsorption onto poly(acrylic acid)-block-polystyrene (PAA₂₂-b-PS₁₄₄) polymersomes has been investigated with regard to structural features, thermodynamic aspects and biological consequences. The light scattering measurements revealed the formation of protein coronas enveloping the polymeric capsules regardless of the chemical nature of the biomacromolecules. The experiments were conducted by using lysozyme, immunoglobulin G - IgG and bovine serum albumin - BSA as model proteins due to their differences concerning size and residual surface charge at physiological pH. The protein adsorption was further confirmed by isothermal titration calorimetry, and the experimental data suggest that the phenomenon is mainly governed by hydrogen bonding and van der Waals interactions. The pre-existing protein layer via the pre-incubation in protein environments notably attenuates the cytotoxicity of the nanomaterial compared to the pristine counterparts. This approach can possibly be extended to different types of assemblies when intermolecular interactions are able to induce protein adsorption and the development of protein coronas around nanoparticles. Such fairly simple method may be convenient to engineer safer nanomaterials towards a variety of biomedical applications when the nanotoxicity is an issue. Additionally, the strategy can possibly be used to tailor the surface properties of nanoparticles by adsorbing specific proteins for targeting purposes.
Keywords	adsorption ; bovine serum albumin ; calorimetry ; case studies ; cytotoxicity ; hydrogen ; immunoglobulin G ; lysozyme ; pH ; thermodynamics ; titration ; van der Waals forces
Language	English
Dates of publication	2022-05
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2022.112387
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Article ; Online: Electrospun blends comprised of poly(methyl methacrylate) and ethyl(hydroxyethyl)cellulose functionalized with perichromic dyes.

Dreyer, Juliana P / Stock, Rafaela I / Nandi, Leandro G / Bellettini, Ismael C / Machado, Vanderlei G

Carbohydrate polymers

2020 Volume 236, Page(s) 115991

Abstract: Ethyl(hydroxyethyl)cellulose (EHEC) was modified with 4-(2- (pyridin-4-yl)vinyl) phenol (PBM) and 4- [4-(dimethylamino)styryl]pyridine (DMASP), which are precursors of two well-known perichromic/fluorescent dyes, Brooker's merocyanine (BM) and 4- [4-( ... ...

Abstract	Ethyl(hydroxyethyl)cellulose (EHEC) was modified with 4-(2- (pyridin-4-yl)vinyl) phenol (PBM) and 4- [4-(dimethylamino)styryl]pyridine (DMASP), which are precursors of two well-known perichromic/fluorescent dyes, Brooker's merocyanine (BM) and 4- [4-(dimethylamino)styryl]-1-methypyridinium iodide (DSMI). The EHECs were mixed with poly(methyl methacrylate) (PMMA) to allow electrospinning and the nanofibers obtained were characterized by scanning electron microscopy, fluorescence microscopy, ATR-FTIR spectroscopy, thermogravimetry analysis, differential scanning calorimetry and fluorescence spectrophotometry and the absolute quantum yield and contact angle were determined. The PMMA/EHEC blends formed hydrophilic nanofibers, with high water absorption, in contrast to the pure PMMA nanofibers. PMMA/EHEC-PBM nanofibers exhibited cyan emission when excited in the UV region and PMMA/EHEC-DMASP showed intense green emission under excitation with laser in the blue region. Electrospinning of PMMA/modified EHEC in the proportion of 5:2 (wt/wt) provided nanochain-like nanofibers, which were verified by confocal microscopy. The potential for the use of the electrospun PMMA/EHEC-PBM nanofibers in the detection of CN
Language	English
Publishing date	2020-02-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2020.115991
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Article: Ready-to-use room temperature one-pot synthesis of surface-decorated gold nanoparticles with targeting attributes

A. S. Ribeiro, Caroline / J. C. Albuquerque, Lindomar / de Castro, Carlos E. / Pereira, Rodrigo M. / Albuquerque, Brunno L. / Pavlova, Ewa / Gabriela Schlüter, Luiza / Batista, Bruno L. / Bellettini, Ismael C. / Giacomelli, Fernando C.

Journal of colloid and interface science. 2022 May 15, v. 614

2022

Abstract: Gold nanoparticles (AuNPs) can be used in diagnostic and therapeutic applications. The development of facile and fast synthetic approaches is accordingly desirable towards ready-to-use biomedical materials. We report a practical one-pot method for the ... ...

Abstract	Gold nanoparticles (AuNPs) can be used in diagnostic and therapeutic applications. The development of facile and fast synthetic approaches is accordingly desirable towards ready-to-use biomedical materials. We report a practical one-pot method for the synthesis in aqueous media and room temperature of surface-decorated AuNPs with enhanced biological responses. The gold ions could be reduced using only polyethyleneimine (PEI) derivatives containing sugar and-or alkyl chains acting simultaneously as reducing and stabilizing agent, without the aid of any other mediator. The process is possibly potentialized by the presence of the amino groups in the polymer chains which further confer colloidal stability. The kinetics of AuNPs nucleation and growth depends on the chemical nature of the polymer chains. Particularly, the presence of lactose moieties conjugated to the PEI chains conducted to surface-decorated AuNPs with low cytotoxicity that are remarkably faster uptaken by HepG2 cells. These cells overexpress asialoglycoprotein (ASGP-R), a galactose receptor. These findings may kick off significant advances towards the practical and ready-to-use manufacturing of functionalized AuNPs towards cell-targeting since the methodology is applicable for a large variety of other ligands that can be conjugated to the same polymer chains.
Keywords	ambient temperature ; cytotoxicity ; galactose ; gold ; lactose ; ligands ; nanogold ; polyethyleneimine ; synthesis ; therapeutics
Language	English
Dates of publication	2022-0515
Size	p. 489-501.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	241597-5
ISSN	1095-7103 ; 0021-9797
ISSN (online)	1095-7103
ISSN	0021-9797
DOI	10.1016/j.jcis.2022.01.145
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Article ; Online: Ready-to-use room temperature one-pot synthesis of surface-decorated gold nanoparticles with targeting attributes.

A S Ribeiro, Caroline / J C Albuquerque, Lindomar / de Castro, Carlos E / Pereira, Rodrigo M / Albuquerque, Brunno L / Pavlova, Ewa / Gabriela Schlüter, Luiza / Batista, Bruno L / Bellettini, Ismael C / Giacomelli, Fernando C

Journal of colloid and interface science

2022 Volume 614, Page(s) 489–501

Abstract	Gold nanoparticles (AuNPs) can be used in diagnostic and therapeutic applications. The development of facile and fast synthetic approaches is accordingly desirable towards ready-to-use biomedical materials. We report a practical one-pot method for the synthesis in aqueous media and room temperature of surface-decorated AuNPs with enhanced biological responses. The gold ions could be reduced using only polyethyleneimine (PEI) derivatives containing sugar and-or alkyl chains acting simultaneously as reducing and stabilizing agent, without the aid of any other mediator. The process is possibly potentialized by the presence of the amino groups in the polymer chains which further confer colloidal stability. The kinetics of AuNPs nucleation and growth depends on the chemical nature of the polymer chains. Particularly, the presence of lactose moieties conjugated to the PEI chains conducted to surface-decorated AuNPs with low cytotoxicity that are remarkably faster uptaken by HepG2 cells. These cells overexpress asialoglycoprotein (ASGP-R), a galactose receptor. These findings may kick off significant advances towards the practical and ready-to-use manufacturing of functionalized AuNPs towards cell-targeting since the methodology is applicable for a large variety of other ligands that can be conjugated to the same polymer chains.
MeSH term(s)	Gold ; Hep G2 Cells ; Humans ; Metal Nanoparticles ; Polyethyleneimine ; Temperature
Chemical Substances	Gold (7440-57-5) ; Polyethyleneimine (9002-98-6)
Language	English
Publishing date	2022-01-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	241597-5
ISSN	1095-7103 ; 0021-9797
ISSN (online)	1095-7103
ISSN	0021-9797
DOI	10.1016/j.jcis.2022.01.145
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Article ; Online: Optical chemosensor for the detection of cyanide in water based on ethyl(hydroxyethyl)cellulose functionalized with Brooker's merocyanine.

Nandi, Leandro G / Nicoleti, Celso R / Bellettini, Ismael C / Machado, Vanderlei G

Analytical chemistry

2014 Volume 86, Issue 10, Page(s) 4653–4656

Abstract: Ethyl(hydroxyethyl)cellulose was functionalized with Brooker's merocyanine. The modified polymer was easily transformed in a film, which could be used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in water, ... ...

Abstract	Ethyl(hydroxyethyl)cellulose was functionalized with Brooker's merocyanine. The modified polymer was easily transformed in a film, which could be used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in water, with detection limits of 1.9 × 10(-5) and 1.0 × 10(-7) mol L(-1). The film was successfully applied to the detection of cyanide in cassava (Manihot esculenta Crantz) roots, which are a well-known source of endogenous biological cyanide.
MeSH term(s)	Benzopyrans/chemistry ; Cellulose/analogs & derivatives ; Cellulose/chemistry ; Cyanides/analysis ; Indoles/chemistry ; Manihot/chemistry ; Water Supply/analysis
Chemical Substances	Benzopyrans ; Cyanides ; Indoles ; ethyl(hydroxyethyl)cellulose ; merocyanine ; Cellulose (9004-34-6)
Language	English
Publishing date	2014-05-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac501233x
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Article: Optical Chemosensor for the Detection of Cyanide in Water Based On Ethyl(hydroxyethyl)cellulose Functionalized with Brookerâs Merocyanine

Nandi, Leandro G / Bellettini Ismael C / Machado Vanderlei G / Nicoleti Celso R

Analytical chemistry. 2014 May 20, v. 86, no. 10

2014

Abstract: Ethyl(hydroxyethyl)cellulose was functionalized with Brookerâs merocyanine. The modified polymer was easily transformed in a film, which could be used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in water, ... ...

Abstract	Ethyl(hydroxyethyl)cellulose was functionalized with Brookerâs merocyanine. The modified polymer was easily transformed in a film, which could be used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in water, with detection limits of 1.9 Ã 10ââµ and 1.0 Ã 10ââ· mol LâÂ¹. The film was successfully applied to the detection of cyanide in cassava (Manihot esculenta Crantz) roots, which are a well-known source of endogenous biological cyanide.
Keywords	cassava ; cellulose ; cyanides ; detection limit ; Manihot esculenta ; polymers ; roots ; sensors (equipment)
Language	English
Dates of publication	2014-0520
Size	p. 4653-4656.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021%2Fac501233x
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Article ; Online: Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression.

Albuquerque, Lindomar J C / Alavarse, Alex C / Carlan da Silva, Maria C / Zilse, Morgana S / Barth, Maitê T / Bellettini, Ismael C / Giacomelli, Fernando C

Macromolecular bioscience

2017 Volume 18, Issue 2

Abstract: The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains ... ...

Abstract	The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency. Accordingly, the preparation of lower-cytotoxicity polyplexes while maintaining gene expression, which is highly relevant to the field, is demonstrated.
MeSH term(s)	Animals ; Benzoxazoles/chemistry ; Cell Death ; Cell Survival ; DNA/metabolism ; Fluorescence ; Gene Expression ; Gene Transfer Techniques ; Genetic Vectors/metabolism ; Green Fluorescent Proteins/metabolism ; HeLa Cells ; Humans ; Macaca mulatta ; Polyethyleneimine/chemistry ; Quinolinium Compounds/chemistry ; Scattering, Radiation ; Sugars/chemistry
Chemical Substances	Benzoxazoles ; Quinolinium Compounds ; Sugars ; 1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium (143413-85-8) ; Green Fluorescent Proteins (147336-22-9) ; Polyethyleneimine (9002-98-6) ; DNA (9007-49-2) ; calf thymus DNA (91080-16-9)
Language	English
Publishing date	2017-12-21
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2039130-4
ISSN	1616-5195 ; 1616-5187
ISSN (online)	1616-5195
ISSN	1616-5187
DOI	10.1002/mabi.201700299
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