LIVIVO - Search results -

Search results

Result 1 - 10 of total 34

Search options

Article ; Online: miRNA-27a-3p is involved in the plasticity of differentiated hepatocytes.

Salerno, Debora / Peruzzi, Giovanna / Giuseppe Rubens Pascucci / Levrero, Massimo / Belloni, Laura / Pediconi, Natalia

2024 Volume 913, Page(s) 148387

Abstract: Background: Epigenetic mechanisms, including DNA methylation, histone modifications, and chromatin remodeling, are highly involved in the regulation of hepatocyte viability, proliferation, and plasticity. We have previously demonstrated that repression ... ...

Abstract	Background: Epigenetic mechanisms, including DNA methylation, histone modifications, and chromatin remodeling, are highly involved in the regulation of hepatocyte viability, proliferation, and plasticity. We have previously demonstrated that repression of H3K27 methylation in differentiated hepatic HepaRG cells by treatment with GSK-J4, an inhibitor of JMJD3 and UTX H3K27 demethylase activity, changed their phenotype, inducing differentiated hepatocytes to proliferate. In addition to the epigenetic enzymatic role in the regulation of the retro-differentiation process, emerging evidence indicate that microRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. Hence, the aim of this work is to investigate the impact of H3K27 methylation on miRNAs expression profile and its role in the regulation of the differentiation status of human hepatic progenitors HepaRG cells. Methods: A miRNA-sequencing was carried out in differentiated HepaRG cells treated or not with GSK-J4. Target searching and Gene Ontology analysis were performed to identify the molecular processes modulated by differentially expressed miRNAs. The biological functions of selected miRNAs was further investigated by transfection of miRNAs inhibitors or mimics in differentiated HepaRG cells followed by qPCR analysis, albumin ELISA assay, CD49a FACS analysis and EdU staining. Results: We identified 12 miRNAs modulated by GSK-J4; among these, miR-27a-3p and miR- 423-5p influenced the expression of several proliferation genes in differentiated HepaRG cells. MiR-27a-3p overexpression increased the number of hepatic cells reentering proliferation. Interestingly, both miR-27a-3p and miR-423-5p did not affect the expression levels of genes involved in the differentiation of progenitors HepaRG cells. Conclusions: Modulation of H3K27me3 methylation in differentiated HepaRG cells, by GSK-J4 treatment, influenced miRNA' s expression profile pushing liver cells towards a proliferating phenotype. We demonstrated the involvement of miR-27a-3p in reinducing proliferation of differentiated hepatocytes suggesting a potential role in liver plasticity.
MeSH term(s)	Humans ; Hepatocytes/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Liver/metabolism ; Cell Differentiation/genetics ; Epigenesis, Genetic
Chemical Substances	MicroRNAs
Language	English
Publishing date	2024-03-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2024.148387
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1357: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article: Reducing the risk of patient suicide in Tuscany.

Dagliana, Giulia / Albolino, Sara / Belloni, Laura / Bellandi, Tommaso

BJPsych international

2020 Volume 17, Issue 4, Page(s) 82–85

Abstract: Patient suicide is one of the most frequent incidents in healthcare facilities to be reported to the National Observatory of Sentinel Events in Italy. Despite national initiatives, in Tuscany potentially preventable patient suicides still occur in both ... ...

Abstract	Patient suicide is one of the most frequent incidents in healthcare facilities to be reported to the National Observatory of Sentinel Events in Italy. Despite national initiatives, in Tuscany potentially preventable patient suicides still occur in both acute and community care settings. We describe here an aggregated qualitative analysis of 14 patient suicides that took place in public health services between 2017 and 2018. We outline the methodology and results of an improvement action we enacted in the healthcare system that involved reviewing and reinforcing relevant managerial strategies and clinical activities, with the aim of reducing potentially preventable patient suicides.
Language	English
Publishing date	2020-11-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2905058-3
ISSN	2058-6264 ; 2056-4740
ISSN (online)	2058-6264
ISSN	2056-4740
DOI	10.1192/bji.2020.43
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: From Development to Aging: The Path to Cellular Senescence.

Sacco, Alessandra / Belloni, Laura / Latella, Lucia

Antioxidants & redox signaling

2020 Volume 34, Issue 4, Page(s) 294–307

Abstract: Significance: ...

Abstract	Significance:
MeSH term(s)	Aging/physiology ; Animals ; Biomarkers ; Cell Cycle Checkpoints ; Cellular Microenvironment ; Cellular Senescence/physiology ; Humans ; Lysosomes/metabolism ; Mitochondria/metabolism
Chemical Substances	Biomarkers
Language	English
Publishing date	2020-05-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1483836-9
ISSN	1557-7716 ; 1523-0864
ISSN (online)	1557-7716
ISSN	1523-0864
DOI	10.1089/ars.2020.8071
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 5488: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Perception of the COVID-19 pandemic among people with spinal cord injury: an Italian survey.

Righi, Gabriele / Baroni, Emanuele / Righi, Lorenzo / Belloni, Laura / Del Popolo, Giulio

Spinal cord series and cases

2022 Volume 8, Issue 1, Page(s) 2

Abstract: Study design: An observational study based on an online survey to explore if the participant had experienced (1) cancellation or delay of scheduled health services (2) reduction of assistance provided by a caregiver (3) barriers to social participation ... ...

Abstract	Study design: An observational study based on an online survey to explore if the participant had experienced (1) cancellation or delay of scheduled health services (2) reduction of assistance provided by a caregiver (3) barriers to social participation and recreational activities. Three validated questionnaires to investigate well-being and symptoms of anxiety and depression were also administered. Objectives: Our main aim was to quantify the obstacles experienced by adults living with SCI in Italy during COVID-19 pandemic, to explore the presence of depression and anxiety symptoms and to quantify subjective well-being. Setting: Outpatient clinic of a Spinal Unit in Italy. Methods: Online survey via direct contact and by e-mail lists. Results: In total, 101 individuals completed the survey. Of, 82.2% participants reported a history of deferment or cancellation of non-COVID-19 health services. The majority (56.4%) revealed that, at least seldom, they have chosen to reduce their usual everyday activities and more than one third (37.6%) affirmed that they had been forced to renounce to one or more of their occupations. Discontinuation of assistance by caregivers was uncommon. The median score of questionnaires measuring depression and anxiety symptoms do not differ significatively when compared with prior studies. The variable that explored the limitations experienced in everyday activities showed a significant correlation with the results of the questionnaires measuring well-being and symptoms of anxiety. Conclusions: We believe that our results could contribute to the discussion ongoing inside our community on how to answer to the new challenges of this pandemic period and of the post-pandemic future.
MeSH term(s)	Adult ; COVID-19 ; Humans ; Pandemics ; Perception ; SARS-CoV-2 ; Spinal Cord Injuries/epidemiology ; Surveys and Questionnaires
Language	English
Publishing date	2022-01-12
Publishing country	England
Document type	Journal Article ; Observational Study
ISSN	2058-6124
ISSN (online)	2058-6124
DOI	10.1038/s41394-021-00470-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: The

Primi, Caterina / Giuli, Monica / Baroni, Emanuele / Zurkirch, Vanessa / Galanti, Matteo / Belloni, Laura / Gori, Costanza / Donati, Maria Anna

International journal of environmental research and public health

2023 Volume 20, Issue 5

Abstract: The validation and psychometric properties of ... ...

Abstract	The validation and psychometric properties of the
MeSH term(s)	Humans ; Psychometrics ; Pandemics ; Cross-Sectional Studies ; Health Personnel ; Workplace ; Reproducibility of Results ; Surveys and Questionnaires
Language	English
Publishing date	2023-02-24
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20054082
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Comparing prognostic utility between the 8th edition of TNM staging system and the lymph node ratio for oral squamous cell carcinoma.

Beltramini, Giada A / Belloni, Laura M / Fusco, Nicola / Sacconi, Andrea / Muti, Paola / Baj, Alessandro / Bolzoni, Alessandro R / Giannì, Aldo B

Head & neck

2021 Volume 43, Issue 10, Page(s) 2876–2882

Abstract: Background: Lymph node metastasis in oral squamous cell carcinoma (OSCC) is associated with poor prognosis. The 8th edition of TNM has implemented new nodal staging criteria. We assess the prognostic utility of the lymph node ratio (LNR) and compare it ... ...

Abstract	Background: Lymph node metastasis in oral squamous cell carcinoma (OSCC) is associated with poor prognosis. The 8th edition of TNM has implemented new nodal staging criteria. We assess the prognostic utility of the lymph node ratio (LNR) and compare it to that of pN in the TNM 8th edition. Methods: One hundred and forty-two patients with OSCC were retrospectively studied. Nodal staging was performed using the TMN 8th edition and the prognostic value of the LNR in terms of overall survival (OS) and disease-free survival (DFS) was evaluated. Results: Fifty-seven patients were eligible for inclusion. The LNR was independently prognostic of OS (p = 0.02). Instead N classification was not significantly predictive of OS (p = 0.10). High LNRs resulted in decreases in OS of approximately 40% within 6 months after surgery. Conclusions: The LNR identifies patients with poor outcomes better than N classification. The lack of reliable LNR cutoffs compromises its utility in clinical practice.
MeSH term(s)	Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/surgery ; Head and Neck Neoplasms ; Humans ; Lymph Node Ratio ; Lymph Nodes/pathology ; Mouth Neoplasms/pathology ; Mouth Neoplasms/surgery ; Neoplasm Staging ; Prognosis ; Retrospective Studies ; Squamous Cell Carcinoma of Head and Neck
Language	English
Publishing date	2021-06-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	645165-2
ISSN	1097-0347 ; 0148-6403 ; 1043-3074
ISSN (online)	1097-0347
ISSN	0148-6403 ; 1043-3074
DOI	10.1002/hed.26769
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1493: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Gender differences in cognitive reserve: implication for subjective cognitive decline in women.

Giacomucci, Giulia / Mazzeo, Salvatore / Padiglioni, Sonia / Bagnoli, Silvia / Belloni, Laura / Ferrari, Camilla / Bracco, Laura / Nacmias, Benedetta / Sorbi, Sandro / Bessi, Valentina

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

2021 Volume 43, Issue 4, Page(s) 2499–2508

Abstract: Background: Subjective Cognitive Decline (SCD) is a self-experienced decline in cognitive capacity with normal performance on standardized cognitive tests, showing to increase risk of Alzheimer's Disease (AD). Cognitive reserve seems to influence the ... ...

Abstract	Background: Subjective Cognitive Decline (SCD) is a self-experienced decline in cognitive capacity with normal performance on standardized cognitive tests, showing to increase risk of Alzheimer's Disease (AD). Cognitive reserve seems to influence the progression from SCD to Mild Cognitive Impairment (MCI) and to AD. The aim of our study was to investigate gender differences in cognitive reserve evaluating how sex might modulate the role of cognitive reserve on SCD. Methods: We included 381 SCD patients who underwent clinical evaluation, neuropsychological assessment, evaluation of premorbid intelligence by the Test di Intelligenza Breve (TIB), cognitive complaints by the Memory Assessment Clinics Questionnaire (MAC-Q), and apolipoprotein E (APOE) genotyping. Results: The proportion between women and men was significantly different (68.7% [95% CI 63.9-73.4 vs 31.4%, 95% CI 26.6-36.0]). Women were younger than men at onset of SCD and at the baseline visit (p = 0.021), had lower years of education (p = 0.007), lower TIB scores (p < 0.001), and higher MAC-Q scores (p = 0.012). TIB was directly associated with age at onset of SCD in both women and men, while years of education was inversely associated with age at onset only in women. Multivariate analysis showed that sex influences TIB independently from years of education. TIB was directly associated with MAC-Q in men. Conclusions: Sex interacts with premorbid intelligence and education level in influencing the age at onset and the severity of SCD. As the effect of education was different between men and women, we speculated that education might act as a minor contributor of cognitive reserve in women.
MeSH term(s)	Alzheimer Disease ; Cognitive Dysfunction/epidemiology ; Cognitive Dysfunction/psychology ; Cognitive Reserve/physiology ; Female ; Humans ; Male ; Neuropsychological Tests ; Sex Factors
Language	English
Publishing date	2021-10-08
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2016546-8
ISSN	1590-3478 ; 1590-1874
ISSN (online)	1590-3478
ISSN	1590-1874
DOI	10.1007/s10072-021-05644-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1877: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Facilitators and barriers in HIV testing and continuum of care among migrant transgender women who are sex workers residing in Florence, Italy.

Lagi, Filippo / Gatteschi, Claudia / Tilli, Marta / Zocco, Nicoletta / Avarello, Angelo / Bellini, Sabrina / Contanessi, Sara / Zigliani, Maria Rosa / Stagnitta, Maria / Mariano, Lorenzo / Gazzarri, Elena / Belloni, Laura / Fisher, Alessandra Daphne / Bartoloni, Alessandro / Sterrantino, Gaetana / Ierardi, Francesca

International journal of transgender health

2023 Volume 25, Issue 2, Page(s) 268–282

Abstract: Background: An increased risk of contracting HIV infection, suboptimal adherence, and a loss to follow-up have been observed in migrants, particularly if those individuals are transgender or sex workers. A clear picture of the HIV epidemic among ... ...

Abstract	Background: An increased risk of contracting HIV infection, suboptimal adherence, and a loss to follow-up have been observed in migrants, particularly if those individuals are transgender or sex workers. A clear picture of the HIV epidemic among migrants is complex due to the lack of specific national data. Aims: We developed a qualitative study that describes the barriers and facilitators (cultural, social, and personal) in HIV testing and the continuum of care for a group of migrant transgender women who are sex workers. Methods: A semi-structured interview was conducted with a group of migrant transgender women who are sex workers living with HIV or with unknown HIV serostatus residing in the Florentine metropolitan area. Results: We included 12 participants: 3 had unknown HIV serostatus and 9 were living with HIV in follow-up at the Clinic of Infectious and Tropical Diseases, Careggi University hospiral, Florence, Italy. Among barriers, the perceived stigma due to their identity as migrants and transgender people, the language lack of ability and the legal position in the host country played a significant role. Moreover, the interviewees claimed having no alternative to sex work: for those individuals, changing their lifestyle condition is perceived as difficult or impossible due to social prejudices. Conversely, the interviewees considered support services, such as cultural mediators/interpreters and street units, as facilitators to HIV testing, access to care, and continuum of care. Having regular and accessible ART and the availability of a more consistent health care system, represent reasons for HIV-positive migrants living with HIV to move to Italy. Conclusions: Knowledge of this population's personal experience regarding the barriers and factors that facilitate access to the HIV care system is essential for planning public health interventions capable of responding to the real needs of patients.
Language	English
Publishing date	2023-05-18
Publishing country	United States
Document type	Journal Article
ISSN	2689-5277
ISSN (online)	2689-5277
DOI	10.1080/26895269.2023.2209072
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Inducing and characterizing vesicular steatosis in differentiated heparg cells

Di Cocco, Silvia / Belloni, Laura / Levrero, Massimo / Nunn, Abigail D.G / Pediconi, Natalia / Piconese, Silvia / Salerno, Debora

Journal of visualized experiments. 2019 July 18, , no. 149

2019

Abstract: Hepatic steatosis represents a metabolic dysfunction that results from an accumulation of triglyceride-containing lipid droplets in hepatocytes. Excessive fat accumulation leads to non-alcoholic fatty liver disease (NAFLD), which is potentially ... ...

Abstract	Hepatic steatosis represents a metabolic dysfunction that results from an accumulation of triglyceride-containing lipid droplets in hepatocytes. Excessive fat accumulation leads to non-alcoholic fatty liver disease (NAFLD), which is potentially reversible and may evolve into non-alcoholic steatohepatitis (NASH) and eventually cirrhosis and hepatocellular carcinoma (HCC). The molecular mechanisms linking lipid accumulation in hepatocytes with the progression to NASH, irreversible liver damage, fibrosis, cirrhosis, and even HCC still remains unclear. To this end, several in vitro and in vivo models have been developed to elucidate the pathological processes that cause NAFLD. In the present study, we describe a cellular model for the induction of liver vesicular steatosis that consists of DMSO-differentiated human hepatic HepaRG cells treated with the fatty acid salt sodium oleate. Indeed, sodium oleate-treated HepaRG cells accumulate lipid droplets in the cytoplasm and show typical features of steatosis. This in vitro human model represents a valuable alternative to in vivo mice models as well as to the primary human hepatocytes. We also present a comparison of several methods for the quantification and evaluation of fat accumulation in HepaRG cells, including Oil Red O staining, cytofluorimetric Bodipy measurement, metabolic gene expression analysis by qPCR, and coherent anti-Stokes Raman scattering (CARS) microscopy. CARS imaging combines the chemical specificity of Raman spectroscopy, a chemical analysis technique well-known in materials science applications, with the benefits of high-speed, high-resolution non-linear optical microscopies to allow precise quantification of lipid accumulation and lipid droplet dynamics. The establishment of an efficient in vitro model for the induction of vesicular steatosis, alongside an accurate method for the quantification and characterization of lipid accumulation, could lead to the development of early stage diagnosis of NAFLD via the identification of molecular markers, and to the generation of new treatment strategies.
Keywords	animal models ; chemical analysis ; cytoplasm ; droplets ; fatty liver ; fibrosis ; gene expression ; genetic markers ; hepatocytes ; hepatoma ; human cell lines ; humans ; image analysis ; liver ; microscopy ; oleic acid ; quantitative polymerase chain reaction ; Raman spectroscopy ; sodium ; staining
Language	English
Dates of publication	2019-0718
Size	p. e59843.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/59843
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: CAG Repeats Within the Non-pathological Range in the HTT Gene Influence Personality Traits in Patients With Subjective Cognitive Decline: A 13-Year Follow-Up Study.

Moschini, Valentina / Mazzeo, Salvatore / Bagnoli, Silvia / Padiglioni, Sonia / Emiliani, Filippo / Giacomucci, Giulia / Morinelli, Carmen / Ingannato, Assunta / Freni, Tommaso / Belloni, Laura / Ferrari, Camilla / Sorbi, Sandro / Nacmias, Benedetta / Bessi, Valentina

Frontiers in psychiatry

2022 Volume 13, Page(s) 826135

Abstract: Objective: HTT: Methods: We included 54 patients with SCD. All patients underwent an extensive neuropsychological battery at baseline, : Results: In the whole sample, CAG repeat number in the shorter : Discussion: Personality traits and CAG ... ...

Abstract	Objective: HTT Methods: We included 54 patients with SCD. All patients underwent an extensive neuropsychological battery at baseline, Results: In the whole sample, CAG repeat number in the shorter Discussion: Personality traits and CAG repeat length in the intermediate range have been associated with progression of cognitive decline and neuropathological findings consistent with AD. We showed that CAG repeat lengths in the
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564218-2
ISSN	1664-0640
ISSN	1664-0640
DOI	10.3389/fpsyt.2022.826135
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito