LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Back to previous search Search history
Advanced search

Your last searches

  1. AU="Belnap, Ethan"
  2. AU="Luo, Jianming"
  3. AU=Hsu Pin-Jui
  4. AU=Debaud Charlotte
  5. AU="García-Rubia, Alfonso"
  6. AU=Belot Alexandre
  7. AU="Jorge Ospina Duque"
  8. AU="Chalot, Michel"
  9. AU="Gunsolley, J N"
  10. AU="Tuè, Giovanni"
  11. AU="de Laat, Bart"
  12. AU=Drory Omri
  13. AU="Pothlichet, Julien"
  14. AU="Douwes, Rianne M"
  15. AU="Jarzebowski, Mary"
  16. AU="Shetty, Sunil"
  17. AU="Ricardo Ryoshim Kunyioshi"
  18. AU=Santos Maria Cristina Leme Godoy Dos AU=Santos Maria Cristina Leme Godoy Dos

Search results

Result 1 - 5 of total 5

Search options

  1. Article: Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA m

    Park, Zachory M / Belnap, Ethan / Remillard, Matthew / Rose, Mark D

    bioRxiv : the preprint server for biology

    2023  

    Abstract: N : Author summary: Ygl036wp is a previously uncharacterized protein that we propose to name Vir1p (budding yeast ortholog of VIR MA/Virilizer/VIR 1 ). Work from our lab and others initially found an interaction between Vir1p and members of the yeast ... ...

    Abstract N
    Author summary: Ygl036wp is a previously uncharacterized protein that we propose to name Vir1p (budding yeast ortholog of VIR MA/Virilizer/VIR 1 ). Work from our lab and others initially found an interaction between Vir1p and members of the yeast mRNA methyltransferase complex (Kar4p and Mum2p). We found that Vir1p interacts with all known members of the methyltransferase complex and is required for mRNA methylation. Vir1p is required early in meiosis;
    Language English
    Publishing date 2023-02-07
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.07.527493
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Kar4 is required for the normal pattern of meiotic gene expression.

    Park, Zachory M / Remillard, Matthew / Belnap, Ethan / Rose, Mark D

    PLoS genetics

    2023  Volume 19, Issue 8, Page(s) e1010898

    Abstract: Kar4p, the yeast homolog of the mammalian methyltransferase subunit METTL14, is required for efficient mRNA m6A methylation, which regulates meiotic entry. Kar4p is also required for a second seemingly non-catalytic function during meiosis. ... ...

    Abstract Kar4p, the yeast homolog of the mammalian methyltransferase subunit METTL14, is required for efficient mRNA m6A methylation, which regulates meiotic entry. Kar4p is also required for a second seemingly non-catalytic function during meiosis. Overexpression of the early meiotic transcription factor, IME1, can bypass the requirement for Kar4p in meiotic entry but the additional overexpression of the translational regulator, RIM4, is required to permit sporulation in kar4Δ/Δ. Using microarray analysis and RNA sequencing, we sought to determine the impact of removing Kar4p and consequently mRNA methylation on the early meiotic transcriptome in a strain background (S288c) that is sensitive to the loss of early meiotic regulators. We found that kar4Δ/Δ mutants have a largely wild type transcriptional profile with the exception of two groups of genes that show delayed and reduced expression: (1) a set of Ime1p-dependent early genes as well as IME1, and (2) a set of late genes dependent on the mid-meiotic transcription factor, Ndt80p. The early gene expression defect is likely the result of the loss of mRNA methylation and is rescued by overexpressing IME1, but the late defect is only suppressed by overexpression of both IME1 and RIM4. The requirement for RIM4 led us to predict that the non-catalytic function of Kar4p, like methyltransferase complex orthologs in other systems, may function at the level of translation. Mass spectrometry analysis identified several genes involved in meiotic recombination with strongly reduced protein levels, but with little to no reduction in transcript levels in kar4Δ/Δ after IME1 overexpression. The low levels of these proteins were rescued by overexpression of RIM4 and IME1, but not by the overexpression of IME1 alone. These data expand our understanding of the role of Kar4p in regulating meiosis and provide key insights into a potential mechanism of Kar4p's later meiotic function that is independent of mRNA methylation.
    MeSH term(s) Animals ; Cytoplasm ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Gene Expression ; Meiosis/genetics ; Methyltransferases/genetics ; RNA, Messenger/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Gene Expression Regulation, Fungal
    Chemical Substances DNA-Binding Proteins ; KAR4 protein, S cerevisiae ; Methyltransferases (EC 2.1.1.-) ; RNA, Messenger ; Saccharomyces cerevisiae Proteins ; Transcription Factors
    Language English
    Publishing date 2023-08-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1010898
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Vir1p, the yeast homolog of virilizer, is required for mRNA m6A methylation and meiosis.

    Park, Zachory M / Belnap, Ethan / Remillard, Matthew / Rose, Mark D

    Genetics

    2023  Volume 224, Issue 1

    Abstract: N6-Methyladenosine (m6A) is among the most abundant modifications of eukaryotic mRNAs. mRNA methylation regulates many biological processes including playing an essential role in meiosis. During meiosis in the budding yeast, Saccharomyces cerevisiae, m6A ...

    Abstract N6-Methyladenosine (m6A) is among the most abundant modifications of eukaryotic mRNAs. mRNA methylation regulates many biological processes including playing an essential role in meiosis. During meiosis in the budding yeast, Saccharomyces cerevisiae, m6A levels peak early, before the initiation of the meiotic divisions. High-throughput studies suggested, and this work confirms that the uncharacterized protein Ygl036wp interacts with Kar4p, a component of the mRNA m6A-methyltransferase complex. Protein structure programs predict that Ygl036wp folds like VIRMA/Virilizer/VIR, which is involved in mRNA m6A-methylation in higher eukaryotes. In addition, Ygl036wp contains conserved motifs shared with VIRMA/Virilizer/VIR. Accordingly, we propose the name VIR1 for budding yeast ortholog of VIRMA/Virilizer/VIR 1. Vir1p interacts with all other members of the yeast methyltransferase complex and is itself required for mRNA m6A methylation and meiosis. In the absence of Vir1p proteins comprising the methyltransferase complex become unstable, suggesting that Vir1p acts as a scaffold for the complex. The vir1Δ/Δ mutant is defective for the premeiotic S-phase, which is suppressed by overexpression of the early meiotic transcription factor IME1; additional overexpression of the translational regulator RIM4 is required for sporulation. The vir1Δ/Δ mutant exhibits reduced levels of IME1 mRNA, as well as transcripts within Ime1p's regulon. Suppression by IME1 revealed an additional defect in the expression of the middle meiotic transcription factor, Ndt80p (and genes in its regulon), which is rescued by overexpression of RIM4. Together, these data suggest that Vir1p is required for cells to initiate the meiotic program and for progression through the meiotic divisions and spore formation.
    MeSH term(s) Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Methylation ; Transcription Factors/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Meiosis/genetics ; Methyltransferases/genetics ; Methyltransferases/metabolism ; RNA-Binding Proteins/genetics
    Chemical Substances Saccharomyces cerevisiae Proteins ; Transcription Factors ; RNA, Messenger ; Methyltransferases (EC 2.1.1.-) ; Rim4 protein, S cerevisiae ; RNA-Binding Proteins
    Language English
    Publishing date 2023-03-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2167-2
    ISSN 1943-2631 ; 0016-6731
    ISSN (online) 1943-2631
    ISSN 0016-6731
    DOI 10.1093/genetics/iyad043
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 767: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Kar4, the Yeast Homolog of METTL14, is Required for mRNA m

    Park, Zachory M / Sporer, Abigail / Kraft, Katherine / Lum, Krystal / Blackman, Edith / Belnap, Ethan / Yellman, Christopher / Rose, Mark D

    bioRxiv : the preprint server for biology

    2023  

    Abstract: KAR4: Author summary: In yeast, ...

    Abstract KAR4
    Author summary: In yeast,
    Language English
    Publishing date 2023-01-29
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.01.29.526094
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.

    Park, Zachory M / Sporer, Abigail J / Kraft, Katherine / Lum, Krystal K / Blackman, Edith / Belnap, Ethan / Yellman, Christopher M / Rose, Mark D

    PLoS genetics

    2023  Volume 19, Issue 8, Page(s) e1010896

    Abstract: KAR4, the yeast homolog of the mammalian mRNA N6A-methyltransferase complex component METTL14, is required for two disparate developmental programs in Saccharomyces cerevisiae: mating and meiosis. To understand KAR4's role in yeast mating and meiosis, we ...

    Abstract KAR4, the yeast homolog of the mammalian mRNA N6A-methyltransferase complex component METTL14, is required for two disparate developmental programs in Saccharomyces cerevisiae: mating and meiosis. To understand KAR4's role in yeast mating and meiosis, we used a genetic screen to isolate 25 function-specific mutant alleles, which map to non-overlapping surfaces on a predicted structure of the Kar4 protein (Kar4p). Most of the mating-specific alleles (Mat-) abolish Kar4p's interaction with the transcription factor Ste12p, indicating that Kar4p's mating function is through Ste12p. In yeast, the mRNA methyltransferase complex was previously defined as comprising Ime4p (Kar4p's paralog and the homolog of mammalian METTL3), Mum2p (homolog of mammalian WTAP), and Slz1p (MIS), but not Kar4p. During meiosis, Kar4p interacts with Ime4p, Mum2p, and Slz1p. Moreover, cells lacking Kar4p have highly reduced levels of mRNA methylation during meiosis indicating that Kar4p is a key member of the methyltransferase complex, as it is in humans. Analysis of kar4Δ/Δ and 7 meiosis-specific alleles (Mei-) revealed that Kar4p is required early in meiosis, before initiation of S-phase and meiotic recombination. High copy expression of the meiotic transcriptional activator IME1 rescued the defect of these Mei- alleles. Surprisingly, Kar4p was also found to be required at a second step for the completion of meiosis and sporulation. Over-expression of IME1 in kar4Δ/Δ permits pre-meiotic S-phase, but most cells remained arrested with a monopolar spindle. Analysis of the function-specific mutants revealed that roughly half became blocked after premeiotic DNA synthesis and did not sporulate (Spo-). Loss of Kar4p's Spo function was suppressed by overexpression of RIM4, a meiotic translational regulator. Overexpression of IME1 and RIM4 together allowed sporulation of kar4Δ/Δ cells. Taken together, these data suggest that Kar4p regulates meiosis at multiple steps, presumably reflecting requirements for methylation in different stages of meiotic gene expression.
    MeSH term(s) Humans ; Meiosis/genetics ; Methylation ; Methyltransferases/genetics ; Reproduction ; Saccharomyces cerevisiae/genetics ; Transcription Factors/genetics ; Saccharomyces cerevisiae Proteins/genetics ; DNA-Binding Proteins/genetics
    Chemical Substances Methyltransferases (EC 2.1.1.-) ; METTL14 protein, human (EC 2.1.1.-) ; METTL3 protein, human (EC 2.1.1.62) ; Transcription Factors ; KAR4 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; DNA-Binding Proteins
    Language English
    Publishing date 2023-08-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1010896
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top