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Article ; Online: Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress.

Jday, Asma / Ben Rejeb, Kilani / Slama, Ines / Saadallah, Kaouthar / Bordenave, Marianne / Planchais, Séverine / Savouré, Arnould / Abdelly, Chedly

Functional plant biology : FPB

2020 Volume 43, Issue 10, Page(s) 939–948

Abstract: Nitric oxide (NO) - an endogenous signalling molecule in plants and animals - mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima ... ...

Abstract	Nitric oxide (NO) - an endogenous signalling molecule in plants and animals - mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima Scop. seedlings under water deficit stress using sodium nitroprusside (SNP) as NO donor and as a pre-treatment before the application of stress. Water deficit stress was applied by withholding water for 14 days. Growth, leaf water content (LWC), osmotic potential (ψs), chlorophyll, malondialdehyde (MDA), electrolyte leakage (EL), proline and Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) protein levels were determined. Enzyme activities involved in antioxidant activities (superoxide dismutase (SOD) and catalase (CAT)) were measured upon withholding water. The results showed that shoot biomass production was significantly decreased in plants subjected to water deficit stress alone. However, in water deficit stressed plants pre-treated with SNP, growth activity was improved and proline accumulation was significantly increased. Proline accumulation was concomitant with the stimulation of its biosynthesis as shown by the accumulation of P5CS proteins. Nevertheless, no significant change in ProDH protein levels was observed. Besides plants showed lower water deficit-induced lipid membrane degradation and oxidative stress after the pretreatment with 100µM SNP. This behaviour was related to the increased activity of SOD and CAT. Thus, we concluded that NO increased C. maritima drought tolerance and mitigated damage associated with water deficit stress by the regulation of proline metabolism and the reduction of oxidative damage.
Language	English
Publishing date	2020-06-02
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2071582-1
ISSN	1445-4416 ; 1445-4408
ISSN (online)	1445-4416
ISSN	1445-4408
DOI	10.1071/FP15363
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Article ; Online: How reactive oxygen species and proline face stress together.

Ben Rejeb, Kilani / Abdelly, Chedly / Savouré, Arnould

Plant physiology and biochemistry : PPB

2014 Volume 80, Page(s) 278–284

Abstract: Reactive oxygen species (ROS) are continuously generated as a consequence of plant metabolic processes due to incomplete reduction of O2. Previously considered to be only toxic by-products of metabolism, ROS are now known to act as second messengers in ... ...

Abstract	Reactive oxygen species (ROS) are continuously generated as a consequence of plant metabolic processes due to incomplete reduction of O2. Previously considered to be only toxic by-products of metabolism, ROS are now known to act as second messengers in intracellular signalling cascades to trigger tolerance of various abiotic and biotic stresses. The accumulation of proline is frequently observed during the exposure of plants to adverse environmental conditions. Interestingly proline metabolism may also contribute to ROS formation in mitochondria, which play notably a role in hypersensitive response in plants, life-span extension in worms and tumor suppression in animals. Here we review current knowledge about the regulation of proline metabolism in response to environmental constraints and highlight the key role of ROS in the regulation of this metabolism. The impact of proline on ROS generation is also investigated. Deciphering and integrating these relationships at the whole plant level will bring new perspectives on how plants adapt to environmental stresses.
MeSH term(s)	Gene Expression Regulation, Plant ; Oxidative Stress/physiology ; Proline/metabolism ; Reactive Oxygen Species/metabolism ; Stress, Physiological
Chemical Substances	Reactive Oxygen Species ; Proline (9DLQ4CIU6V)
Language	English
Publishing date	2014-04-23
Publishing country	France
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2014.04.007
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Article ; Online: La proline, un acide aminé multifonctionnel impliqué dans l'adaptation des plantes aux contraintes environnementales.

Ben Rejeb, Kilani / Abdelly, Chedly / Savouré, Arnould

Biologie aujourd'hui

2012 Volume 206, Issue 4, Page(s) 291–299

Abstract: In addition to its role in primary metabolism as a component of proteins, proline is one of the most widely distributed compatible solutes that accumulates in plants during adverse environmental constraints and plays an important role in plant stress ... ...

Title translation	Proline, a multifunctional amino-acid involved in plant adaptation to environmental constraints.
Abstract	In addition to its role in primary metabolism as a component of proteins, proline is one of the most widely distributed compatible solutes that accumulates in plants during adverse environmental constraints and plays an important role in plant stress tolerance. Proline was proposed to act as stabilizer for proteins and macromolecular complexes, scavenger of free radicals and regulator of cellular redox potential. Intracellular proline concentration depends on a tight regulation between its biosynthesis and catabolism. However the exact role of proline and the signaling pathways involved in the regulation of its metabolism are not completely known yet. Investigation of proline metabolism in model plants would allow to acquire information about the diversity of the mechanisms developed by plants to overcome environmental constraints and to establish some reliable tools for the improvement of crop tolerance.
MeSH term(s)	Adaptation, Physiological ; Environment ; Plant Physiological Phenomena ; Plants/metabolism ; Proline/metabolism ; Proline/pharmacology ; Proline/physiology ; Signal Transduction ; Stress, Physiological/physiology
Chemical Substances	Proline (9DLQ4CIU6V)
Language	French
Publishing date	2012
Publishing country	France
Document type	English Abstract ; Journal Article ; Review
ISSN	2105-0686
ISSN (online)	2105-0686
DOI	10.1051/jbio/2012030
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Article: NADPH oxidase-dependent H2O2 production is required for salt-induced antioxidant defense in Arabidopsis thaliana

Ben Rejeb, Kilani / Ahmed Debez / Arnould Savouré / Chedly Abdelly / Christophe Bailly / Maâli Benzarti

Journal of plant physiology. 2015 Feb. 01, v. 174

2015

Abstract: The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. In the wild-type, short-term ... ...

Abstract	The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. In the wild-type, short-term salt exposure led to a transient and significant increase of H2O2 concentration, followed by a marked increase in catalase (CAT, EC 1.11.16), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) activities. Pre-treatment with either a chemical trap for H2O2 (dimethylthiourea) or two widely used NADPH oxidase inhibitors (imidazol and diphenylene iodonium) significantly decreased the above-mentioned enzyme activities under salinity. Double mutant atrbohd/f plants failed to induce the antioxidant response under the culture conditions. Under long-term salinity, the wild-type was more salt-tolerant than the mutant based on the plant biomass production. The better performance of the wild-type was related to a significantly higher photosynthetic activity, a more efficient K+ selective uptake, and to the plants’ ability to deal with the salt-induced oxidative stress as compared to atrbohd/f. Altogether, these data suggest that the early H2O2 generation by NADPH oxidase under salt stress could be the beginning of a reaction cascade that triggers the antioxidant response in A. thaliana in order to overcome the subsequent reactive oxygen species (ROS) production, thereby mitigating the salt stress-derived injuries.
Keywords	antioxidant activity ; Arabidopsis thaliana ; ascorbate peroxidase ; biomass production ; catalase ; enzyme activity ; glutathione-disulfide reductase ; hydrogen peroxide ; mutants ; NAD (coenzyme) ; NAD(P)H oxidase (H2O2-forming) ; NADP (coenzyme) ; oxidative stress ; photosynthesis ; phytomass ; potassium ; salinity ; salt stress ; salt tolerance ; seedlings
Language	English
Dates of publication	2015-0201
Size	p. 5-15.
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	283647-6
ISSN	1618-1328 ; 0176-1617
ISSN (online)	1618-1328
ISSN	0176-1617
DOI	10.1016/j.jplph.2014.08.022
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Article: How reactive oxygen species and proline face stress together

Ben Rejeb, Kilani / Arnould SavourÃ© / Chedly Abdelly

Plant physiology and biochemistry. 2014 July, v. 80

2014

Abstract	Reactive oxygen species (ROS) are continuously generated as a consequence of plant metabolic processes due to incomplete reduction of O2. Previously considered to be only toxic by-products of metabolism, ROS are now known to act as second messengers in intracellular signalling cascades to trigger tolerance of various abiotic and biotic stresses. The accumulation of proline is frequently observed during the exposure of plants to adverse environmental conditions. Interestingly proline metabolism may also contribute to ROS formation in mitochondria, which play notably a role in hypersensitive response in plants, life-span extension in worms and tumor suppression in animals. Here we review current knowledge about the regulation of proline metabolism in response to environmental constraints and highlight the key role of ROS in the regulation of this metabolism. The impact of proline on ROS generation is also investigated. Deciphering and integrating these relationships at the whole plant level will bring new perspectives on how plants adapt to environmental stresses.
Keywords	animals ; biotic stress ; byproducts ; environmental factors ; hypersensitive response ; metabolism ; mitochondria ; oxygen ; proline ; reactive oxygen species ; second messengers ; toxicity
Language	English
Dates of publication	2014-07
Size	p. 278-284.
Publishing place	Elsevier Masson SAS
Document type	Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2014.04.007
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Article ; Online: NADPH oxidase-dependent H2O2 production is required for salt-induced antioxidant defense in Arabidopsis thaliana.

Ben Rejeb, Kilani / Benzarti, Maâli / Debez, Ahmed / Bailly, Christophe / Savouré, Arnould / Abdelly, Chedly

Journal of plant physiology

2014 Volume 174, Page(s) 5–15

Abstract	The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. In the wild-type, short-term salt exposure led to a transient and significant increase of H2O2 concentration, followed by a marked increase in catalase (CAT, EC 1.11.16), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) activities. Pre-treatment with either a chemical trap for H2O2 (dimethylthiourea) or two widely used NADPH oxidase inhibitors (imidazol and diphenylene iodonium) significantly decreased the above-mentioned enzyme activities under salinity. Double mutant atrbohd/f plants failed to induce the antioxidant response under the culture conditions. Under long-term salinity, the wild-type was more salt-tolerant than the mutant based on the plant biomass production. The better performance of the wild-type was related to a significantly higher photosynthetic activity, a more efficient K(+) selective uptake, and to the plants' ability to deal with the salt-induced oxidative stress as compared to atrbohd/f. Altogether, these data suggest that the early H2O2 generation by NADPH oxidase under salt stress could be the beginning of a reaction cascade that triggers the antioxidant response in A. thaliana in order to overcome the subsequent reactive oxygen species (ROS) production, thereby mitigating the salt stress-derived injuries.
MeSH term(s)	Antioxidants/metabolism ; Arabidopsis/drug effects ; Arabidopsis/enzymology ; Arabidopsis/genetics ; Arabidopsis/physiology ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Ascorbate Peroxidases/metabolism ; Catalase/metabolism ; Fluoresceins/metabolism ; Gene Expression Regulation, Plant/drug effects ; Glutathione Reductase/metabolism ; Hydrogen Peroxide/metabolism ; Imidazoles/pharmacology ; Malondialdehyde/metabolism ; Mutation/genetics ; NADPH Oxidases/genetics ; NADPH Oxidases/metabolism ; Onium Compounds/pharmacology ; Sodium Chloride/pharmacology ; Stress, Physiological/drug effects ; Thiourea/analogs & derivatives ; Thiourea/pharmacology ; Time Factors
Chemical Substances	Antioxidants ; Arabidopsis Proteins ; Fluoresceins ; Imidazoles ; Onium Compounds ; diacetyldichlorofluorescein (2044-85-1) ; Sodium Chloride (451W47IQ8X) ; Malondialdehyde (4Y8F71G49Q) ; diphenyleneiodonium (6HJ411TU98) ; imidazole (7GBN705NH1) ; 1,3-dimethylthiourea (8P30PMD17W) ; Hydrogen Peroxide (BBX060AN9V) ; Ascorbate Peroxidases (EC 1.11.1.11) ; Catalase (EC 1.11.1.6) ; NADPH Oxidases (EC 1.6.3.-) ; AtrbohF protein, Arabidopsis (EC 1.6.3.1) ; RbohF protein, Arabidopsis (EC 1.6.3.1) ; Glutathione Reductase (EC 1.8.1.7) ; Thiourea (GYV9AM2QAG)
Language	English
Publishing date	2014-10-23
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	283647-6
ISSN	1618-1328 ; 0176-1617
ISSN (online)	1618-1328
ISSN	0176-1617
DOI	10.1016/j.jplph.2014.08.022
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Article: Photosynthetic activity and leaf antioxidative responses of Atriplex portulacoides subjected to extreme salinity

Benzarti, Maali / Ben Rejeb, Kilani / Debez, Ahmed / Messedi, Dorsaf / Abdelly, Chedly

Acta physiologiae plantarum. 2012 Sept., v. 34, no. 5

2012

Abstract: Responses of Atriplex portulacoides upon 40-day-long exposure to salinity (0–1,000 mM NaCl) were investigated. Mother plants originated from a sabkha located in a semi-arid region of Tunisia. The plant relative growth rate and leaf expansion increased ... ...

Abstract	Responses of Atriplex portulacoides upon 40-day-long exposure to salinity (0–1,000 mM NaCl) were investigated. Mother plants originated from a sabkha located in a semi-arid region of Tunisia. The plant relative growth rate and leaf expansion increased significantly at 200 mM NaCl but decreased at higher salinities. Interestingly, the plants survived salinity as high as 1,000 mM NaCl without displaying salt-induced toxicity symptoms. Despite significant increase in leaf Na+ and Cl− concentrations upon salt treatment, no significant effect on leaf relative water content was registered. Chlorophyll contents and the gas exchange parameters showed a significant stimulation at the optimal salinity (200 mM NaCl) followed by a decline at higher salinities. Extreme salinity hardly impacted the maximal efficiency of photosystem II photochemistry (F v/F m), but a marked decrease in the relative quantum yield of photosystem II (ФPSII) was observed, along with a significant increase in non-photochemical quenching (NPQ). Leaf malondialdehyde and carotenoid contents were generally unaffected following salt exposure, whereas those of anthocyanins, polyphenols, and proline increased significantly, being maximal at 1,000 mM NaCl. Leaf superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11), and glutathione reductase (EC 1.6.4.2) activities were significantly stimulated by salinity, whereas catalase (EC 1.11.1.6) activity was maximal in the 0–400 mM NaCl range. As a whole, protecting the photosynthetic machinery from salt-induced photodamage together with the sustained antioxidant activity may account for the performance of A. portulacoides under high salinity.
Keywords	Atriplex ; antioxidant activity ; ascorbate peroxidase ; catalase ; chlorophyll ; gas exchange ; glutathione-disulfide reductase ; leaves ; malondialdehyde ; photochemistry ; photosystem II ; polyphenols ; proline ; salinity ; semiarid zones ; sodium chloride ; superoxide dismutase ; toxicity ; water content
Language	English
Dates of publication	2012-09
Size	p. 1679-1688.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	783102-x
ISSN	1861-1664 ; 0137-5881
ISSN (online)	1861-1664
ISSN	0137-5881
DOI	10.1007/s11738-012-0963-5
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Article: Effects of EDTA on phytoextraction of heavy metals (Zn, Mn and Pb) from sludge-amended soil with Brassica napus

Zaier, Hanen / Ghnaya, Tahar / Ben Rejeb, Kilani / Lakhdar, Abdelbasset / Rejeb, Salwa / Jemal, Fatima

Bioresource technology. 2010 June, v. 101, no. 11

2010

Abstract: Sludge application is a reliable practice to ameliorate soil fertility. However, repetitive sludge addition represents a potential soil contamination source with heavy metals, which must be extracted. The aim of this study was to evaluate the capacity of ...

Abstract	Sludge application is a reliable practice to ameliorate soil fertility. However, repetitive sludge addition represents a potential soil contamination source with heavy metals, which must be extracted. The aim of this study was to evaluate the capacity of Brassica napus to remove metals from soils amended with sludge, and to study the effect of EDTA on this process. Seedlings were cultivated in presence of sludge combined or not with EDTA. Results showed that sludge ameliorate significantly biomass production. This effect was accompanied with an increase in Pb, Zn and Mn shoot concentrations. EDTA application does not affect significantly plant growth. However, this chelator enhances shoot metals accumulation. It's therefore concluded that sludge has a beneficial effect on soil fertility, B. napus can be used for the decontamination of affected soils and that the EDTA addition increases the ability of B. napus to accumulate heavy metals.
Keywords	sewage sludge ; soil pollution ; pollutants ; zinc ; lead ; manganese ; heavy metals ; phytoremediation ; EDTA (chelating agent) ; chelating agents ; soil amendments ; Brassica napus
Language	English
Dates of publication	2010-06
Size	p. 3978-3983.
Publishing place	[New York, NY]: Elsevier Ltd.
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2010.01.035
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Article ; Online: Effects of EDTA on phytoextraction of heavy metals (Zn, Mn and Pb) from sludge-amended soil with Brassica napus.

Zaier, Hanen / Ghnaya, Tahar / Ben Rejeb, Kilani / Lakhdar, Abdelbasset / Rejeb, Salwa / Jemal, Fatima

Bioresource technology

2010 Volume 101, Issue 11, Page(s) 3978–3983

Abstract	Sludge application is a reliable practice to ameliorate soil fertility. However, repetitive sludge addition represents a potential soil contamination source with heavy metals, which must be extracted. The aim of this study was to evaluate the capacity of Brassica napus to remove metals from soils amended with sludge, and to study the effect of EDTA on this process. Seedlings were cultivated in presence of sludge combined or not with EDTA. Results showed that sludge ameliorate significantly biomass production. This effect was accompanied with an increase in Pb, Zn and Mn shoot concentrations. EDTA application does not affect significantly plant growth. However, this chelator enhances shoot metals accumulation. It's therefore concluded that sludge has a beneficial effect on soil fertility, B. napus can be used for the decontamination of affected soils and that the EDTA addition increases the ability of B. napus to accumulate heavy metals.
MeSH term(s)	Biomass ; Brassica napus/growth & development ; Brassica napus/metabolism ; Chelating Agents/chemistry ; Edetic Acid/chemistry ; Metals, Heavy/isolation & purification ; Sewage ; Soil Pollutants/isolation & purification
Chemical Substances	Chelating Agents ; Metals, Heavy ; Sewage ; Soil Pollutants ; Edetic Acid (9G34HU7RV0)
Language	English
Publishing date	2010-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2010.01.035
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Article ; Online: Hydrogen peroxide produced by NADPH oxidases increases proline accumulation during salt or mannitol stress in Arabidopsis thaliana.

Ben Rejeb, Kilani / Lefebvre-De Vos, Delphine / Le Disquet, Isabel / Leprince, Anne-Sophie / Bordenave, Marianne / Maldiney, Régis / Jdey, Asma / Abdelly, Chedly / Savouré, Arnould

The New phytologist

2015 Volume 208, Issue 4, Page(s) 1138–1148

Abstract: Many plants accumulate proline, a compatible osmolyte, in response to various environmental stresses such as water deficit and salinity. In some stress responses, plants generate hydrogen peroxide (H2 O2 ) that mediates numerous physiological and ... ...

Abstract	Many plants accumulate proline, a compatible osmolyte, in response to various environmental stresses such as water deficit and salinity. In some stress responses, plants generate hydrogen peroxide (H2 O2 ) that mediates numerous physiological and biochemical processes. The aim was to study the relationship between stress-induced proline accumulation and H2 O2 production. Using pharmacological and reverse genetic approaches in Arabidopsis thaliana, we investigated the role of NADPH oxidases, Respiratory burst oxidase homologues (Rboh), in the induction of proline accumulation was investigated in response to stress induced by either 200 mM NaCl or 400 mM mannitol. Stress from NaCl or mannitol resulted in a transient increase in H2 O2 content accompanied by accumulation of proline. Dimethylthiourea, a scavenger of H2 O2 , and diphenylene iodonium (DPI), an inhibitor of H2 O2 production by NADPH oxidase, were found to significantly inhibit proline accumulation in these stress conditions. DPI also reduced the expression level of Δ(1) -pyrroline-5-carboxylate synthetase, the key enzyme involved in the biosynthesis of proline. Similarly, less proline accumulated in knockout mutants lacking either AtRbohD or AtRbohF than in wild-type plants in response to the same stresses. Our data demonstrate that AtRbohs (A. thaliana Rbohs) contribute to H2 O2 production in response to NaCl or mannitol stress to increase proline accumulation in this plant.
MeSH term(s)	Adaptation, Physiological ; Arabidopsis/metabolism ; Arabidopsis Proteins/metabolism ; Hydrogen Peroxide/metabolism ; Mannitol/metabolism ; NADH, NADPH Oxidoreductases/metabolism ; NADPH Oxidases/metabolism ; Proline/metabolism ; Sodium Chloride/metabolism ; Stress, Physiological
Chemical Substances	Arabidopsis Proteins ; Mannitol (3OWL53L36A) ; Sodium Chloride (451W47IQ8X) ; Proline (9DLQ4CIU6V) ; Hydrogen Peroxide (BBX060AN9V) ; NADH, NADPH Oxidoreductases (EC 1.6.-) ; NADPH Oxidases (EC 1.6.3.-) ; superoxide-forming enzyme (EC 1.6.99.-)
Language	English
Publishing date	2015-07-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.13550
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