LIVIVO - Search results -

Search results

Result 1 - 10 of total 52

Search options

Article: Changes in inoculated

Ben Hsouna, Anis / Čmiková, Natália / Ben Akacha, Boutheina / Ben Saad, Rania / Mnif, Wissem / Garzoli, Stefania / Kačániová, Miroslava

Heliyon

2024 Volume 10, Issue 7, Page(s) e29065

Abstract: After harvesting, pathogens can infect fresh vegetables in different ways. Pathogenic bacteria associated with fresh vegetables can cause widespread epidemics associated with foodborne illness. The aim of this study was to assess the microbiological ... ...

Abstract	After harvesting, pathogens can infect fresh vegetables in different ways. Pathogenic bacteria associated with fresh vegetables can cause widespread epidemics associated with foodborne illness. The aim of this study was to assess the microbiological quality of carrot slices after treatment with aqueous extracts of
Language	English
Publishing date	2024-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e29065
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Functional Characterization of

Ben Saad, Rania / Ben Romdhane, Walid / Baazaoui, Narjes / Bouteraa, Mohamed Taieb / Chouaibi, Yosra / Mnif, Wissem / Ben Hsouna, Anis / Kačániová, Miroslava

International journal of molecular sciences

2023 Volume 24, Issue 3

Abstract: Cold stress is a key environmental factor affecting plant growth and development, crop productivity, and geographic distribution. Thioredoxins (Trxs) are small proteins that are ubiquitously expressed in all organisms and implicated in several cellular ... ...

Abstract	Cold stress is a key environmental factor affecting plant growth and development, crop productivity, and geographic distribution. Thioredoxins (Trxs) are small proteins that are ubiquitously expressed in all organisms and implicated in several cellular processes, including redox reactions. However, their role in the regulation of cold stress in the halophyte plant
MeSH term(s)	Antioxidants/metabolism ; Brassicaceae/genetics ; Cell Membrane/metabolism ; Gene Expression Regulation, Plant ; Hydrogen Peroxide/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Reactive Oxygen Species/metabolism ; Salt-Tolerant Plants/genetics ; Stress, Physiological/genetics ; Nicotiana/metabolism ; Cold Temperature
Chemical Substances	Antioxidants ; Hydrogen Peroxide (BBX060AN9V) ; Plant Proteins ; Reactive Oxygen Species
Language	English
Publishing date	2023-02-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24033030
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Biopreservative Effect of the Tunisian Halophyte

Ben Akacha, Boutheina / Garzoli, Stefania / Ben Saad, Rania / Brini, Faical / Mnif, Wissem / Kačániová, Miroslava / Ben Hsouna, Anis

Metabolites

2023 Volume 13, Issue 3

Abstract: In the present study, ...

Abstract	In the present study,
Language	English
Publishing date	2023-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo13030371
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Citrus limon

Kačániová, Miroslava / Čmiková, Natália / Vukovic, Nenad L / Verešová, Andrea / Bianchi, Alessandro / Garzoli, Stefania / Ben Saad, Rania / Ben Hsouna, Anis / Ban, Zhaojun / Vukic, Milena D

Plants (Basel, Switzerland)

2024 Volume 13, Issue 4

Abstract: New goals for industry and science have led to increased awareness of food safety and healthier living in the modern era. Here, one of the challenges in food quality assurance is the presence of pathogenic microorganisms. As planktonic cells can form ... ...

Abstract	New goals for industry and science have led to increased awareness of food safety and healthier living in the modern era. Here, one of the challenges in food quality assurance is the presence of pathogenic microorganisms. As planktonic cells can form biofilms and go into a sessile state, microorganisms are now more resistant to broad-spectrum antibiotics. Due to their proven antibacterial properties, essential oils represent a potential option to prevent food spoilage in the search for effective natural preservatives. In this study, the chemical profile of
Language	English
Publishing date	2024-02-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants13040524
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Assessment of the phytochemical composition, antimicrobial activity and anti-inflammatory effects of Lobularia maritima extracts on lipopolysaccharide-stimulated RAW 264.7 cells and their capacity to extend the shelf life of raw minced beef

Ben Hsouna, Anis / Michalak, Monika / Ben Akacha, Boutheina / Dhifi, Wissal / Ben Saad, Rania / Brini, Faiçal / Mnif, Wissem

Journal of Functional Foods. 2022 Dec., v. 99 p.105327-

2022

Abstract: This study aims to investigate the phytochemical composition of various extracts (hexane, dichloromethane, ethyl acetate, and aqueous) of L. maritima. The extracts were tested for their anti-inflammatory and antimicrobial properties and to demonstrate ... ...

Abstract	This study aims to investigate the phytochemical composition of various extracts (hexane, dichloromethane, ethyl acetate, and aqueous) of L. maritima. The extracts were tested for their anti-inflammatory and antimicrobial properties and to demonstrate the potential use of L. maritima extract, as a source of natural antioxidants, to preserve meat quality. The aqueous extract (AE) was shown to be the richest in phenols and flavonoids. AE decreased the expression of pro-inflammatory cytokines and inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells, which may suggest its anti-inflammatory activity. In addition, AE exhibited antibacterial potential, which can be exploited to extend the shelf life of raw minced beef and improve its quality. The addition of L. maritima extract to meat was shown to have a significant effect on its microbiological, physicochemical and sensory properties.
Keywords	Lobularia maritima ; anti-inflammatory activity ; antibacterial properties ; chemical constituents of plants ; cytokines ; ethyl acetate ; flavonoids ; hexane ; meat quality ; methylene chloride ; minced beef ; shelf life ; Phenolic composition ; Antimicrobial activity ; Shelf life extension ; AE ; APC ; BHT ; cDNA ; COX-2 ; DE ; DMEM ; dNTP ; EAE ; ESI-MS ; FBS ; GAE ; GAPDH ; HE ; IC50 ; IFN-γ ; IL-1β ; IL-6 ; LC-MS ; LPS ; MBC ; MDA ; MetMb ; MIC ; MTT ; NO ; NOS ; PBS ; PCR ; PTC ; RNA ; TBARS ; TFC ; TNF-α ; TPC ; VRBG
Language	English
Dates of publication	2022-12
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2511964-3
ISSN	1756-4646
ISSN	1756-4646
DOI	10.1016/j.jff.2022.105327
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 7830: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system

Ben Saad, Rania / Ben Romdhane, Walid / Bouteraa, Mohamed Taieb / Jrad, Olfa / Ben Hsouna, Anis

Plant growth regulation. 2022 May, v. 97, no. 1

2022

Abstract: Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. ... ...

Abstract	Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM H₂O₂) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance.
Keywords	Lobularia maritima ; chloroplast thioredoxins ; computer simulation ; confocal microscopy ; growth and development ; multigene family ; osmotic stress ; phylogeny ; plant growth ; plant improvement ; plasma membrane ; sequence analysis ; stress tolerance ; tobacco ; transgenic plants
Language	English
Dates of publication	2022-05
Size	p. 101-115.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	843025-1
ISSN	1573-5087 ; 0167-6903
ISSN (online)	1573-5087
ISSN	0167-6903
DOI	10.1007/s10725-022-00805-0
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 3711: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: The durum wheat annexin, TdAnn6, improves salt and osmotic stress tolerance in Arabidopsis via modulation of antioxidant machinery

Harbaoui, Marwa / Ben Romdhane, Walid / Ben Hsouna, Anis / Brini, Faiçal / Ben Saad, Rania

Protoplasma. 2021 Sept., v. 258, no. 5

2021

Abstract: TdAnn6 is a gene encoding an annexin protein in durum wheat (Triticum durum). The function of TdAnn6 in plant response to stress is not yet clearly understood. Here, we isolated TdAnn6 and characterized it in genetically modified Arabidopsis thaliana. ... ...

Abstract	TdAnn6 is a gene encoding an annexin protein in durum wheat (Triticum durum). The function of TdAnn6 in plant response to stress is not yet clearly understood. Here, we isolated TdAnn6 and characterized it in genetically modified Arabidopsis thaliana. Expressing TdAnn6 in Arabidopsis coincided with an improvement in stress tolerance at germination and seedling stages. In addition, TdAnn6-expressing seedling antioxidant activities were improved with lower level of malondialdehyde, and enhanced transcript levels of six stress-related genes during salt/osmotic stresses. Under greenhouse conditions, the TdAnn6 plants exhibited increased tolerance to salt or drought stress. To deepen our understanding of TdAnn6 function, we isolated a 1515-bp genomic fragment upstream of its coding sequence, designated as PrTdAnn6. The PrTdAnn6 promoter was fused to the β-glucuronidase reporter gene and transferred to Arabidopsis. By histochemical GUS staining, GUS activity was detected in the roots, leaves, and floral organs, but no activity was detected in the seeds. Furthermore, we noticed a high stimulation of promoter activity when A. thaliana seedlings were exposed to NaCl, mannitol, ABA, GA, and cold conditions. This cross-talk between tissue-specific expression and exogenous stress stimulation may provide additional layers of regulation for salt and osmotic stress responses in crops.
Keywords	Arabidopsis thaliana ; Triticum turgidum subsp. durum ; antioxidants ; cold ; durum wheat ; genomics ; germination ; greenhouses ; malondialdehyde ; mannitol ; osmotic stress ; plant response ; reporter genes ; salt tolerance ; seedlings ; stress response ; stress tolerance ; water stress
Language	English
Dates of publication	2021-09
Size	p. 1047-1059.
Publishing place	Springer Vienna
Document type	Article
ZDB-ID	123809-7
ISSN	1615-6102 ; 0033-183X
ISSN (online)	1615-6102
ISSN	0033-183X
DOI	10.1007/s00709-021-01622-4
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 46/342: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Recent Advances in the Incorporation of Polysaccharides with Antioxidant and Antibacterial Functions to Preserve the Quality and Shelf Life of Meat Products

Ben Akacha, Boutheina / Michalak, Monika / Najar, Basma / Venturi, Francesca / Taglieri, Isabella / Kačániová, Miroslava / Ben Saad, Rania / Mnif, Wissem / Garzoli, Stefania / Ben Hsouna, Anis

Foods. 2023 Apr. 14, v. 12, no. 8

2023

Abstract: Meat and meat products are susceptible to various types of natural processes such as oxidative degradation due to their high content of protein and essential amino acids. However, finding solutions to maintain the nutritional and sensory quality of meat ... ...

Abstract	Meat and meat products are susceptible to various types of natural processes such as oxidative degradation due to their high content of protein and essential amino acids. However, finding solutions to maintain the nutritional and sensory quality of meat and meat products is unavoidable. Hence, there is a pressing need to investigate alternatives to synthetic preservatives, focusing on active biomolecules of natural provenance. Polysaccharides are natural polymers of various sources that exhibit antibacterial and antioxidant properties via a variety of mechanisms, owing to their diversity and structural variation. For this reason, these biomolecules are widely studied in order to improve texture, inhibit the growth of pathogens, and improve the oxidative stability and sensory characteristics of meat products. However, the literature has not addressed their biological activity in meat and meat products. This review summarizes the various sources of polysaccharides, their antioxidant and antibacterial activities (mainly against pathogenic food strains), and their use as natural preservatives to replace synthetic additives in meat and meat products. Special attention is given to the use of polysaccharides to improve the nutritional value of meat, resulting in more nutrient-rich meat products with higher polysaccharide content and less salt, nitrites/nitrates, and cholesterol.
Keywords	antioxidants ; bioactive properties ; cholesterol ; meat ; nutritive value ; oxidative stability ; polysaccharides ; provenance ; sensory properties ; shelf life ; texture
Language	English
Dates of publication	2023-0414
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12081647
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Assessment of the Cadmium and Copper Phytoremediation Potential of the Lobularia maritima Thioredoxin 2 Gene Using Genetically Engineered Tobacco

Ben Saad, Rania / Ben Romdhane, Walid / Baazaoui, Narjes / Bouteraa, Mohamed Taieb / Ben Hsouna, Anis / Mishra, Avinash / Ćavar Zeljković, Sanja

Agronomy. 2023 Jan. 30, v. 13, no. 2

2023

Abstract: Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of ... ...

Abstract	Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that plays a crucial role in intracellular redox homeostasis in both eukaryotes and prokaryotes. Herein, the presence of heavy metals highly upregulated LmTrxh2 transcription in Lobularia maritima seedlings and its overexpression-conferred tolerance to Cd, Cu, Mn, and Zn in Saccharomyces cerevisiae. In addition, LmTrxh2-overexpressing tobacco plants had higher seedling survival rates than non-transgenic plants (NT), with enhanced root length and biomass production and reduced ROS accumulation, following Cd and Cu stress. These plants also accumulated more Cd, Cu, and Mn than the NT plants. Moreover, LmTrxh2 overexpression stimulated the transcription of genes encoding metallothioneins (Met-1, Met-2, Met-3, and Met-4), a copper transport protein, a Snakin/GASA protein (Snakin-2), and ROS-scavenging enzymes (SOD, APX1, and CAT), which might contribute to heavy metal tolerance in tobacco plants. These results suggest that LmTrxh2 overexpression helps to improve heavy metal tolerance by stimulating antioxidant capacities and the expression of several stress-responsive genes in plants.
Keywords	Lobularia maritima ; Saccharomyces cerevisiae ; agronomy ; biomass production ; cadmium ; copper ; disulfides ; eukaryotic cells ; genes ; genetic engineering ; homeostasis ; industrialization ; metal tolerance ; modernization ; oxidative stress ; oxidoreductases ; phytoremediation ; prokaryotic cells ; reactive oxygen species ; seedlings ; soil pollution ; thioredoxins ; tobacco ; transport proteins ; urbanization
Language	English
Dates of publication	2023-0130
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2607043-1
ISSN	2073-4395
ISSN	2073-4395
DOI	10.3390/agronomy13020399
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Novel non-specific lipid-transfer protein (TdLTP4) isolated from durum wheat: Antimicrobial activities and anti-inflammatory properties in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.

Ben Hsouna, Anis / Ben Saad, Rania / Dhifi, Wissal / Mnif, Wissem / Brini, Faiçal

Microbial pathogenesis

2021 Volume 154, Page(s) 104869

Abstract: Lipid transfer proteins (LTP) are members of the family of pathogenesis-related proteins (PR-14) that play a key role in plant defense mechanisms. In this study, a novel gene TdLTP4 encoding an antifungal protein from wheat (cv. Om Rabiaa) was cloned, ... ...

Abstract	Lipid transfer proteins (LTP) are members of the family of pathogenesis-related proteins (PR-14) that play a key role in plant defense mechanisms. In this study, a novel gene TdLTP4 encoding an antifungal protein from wheat (cv. Om Rabiaa) was cloned, overexpressed in Escherichia coli BL-21 (DE3) and enriched using ammonium sulfate fractionation. The TdLTP4 fusion protein was then tested against a panel of pathogens, food-borne and spoilage bacteria and fungi in order to evaluate the antimicrobial properties. TdLTP4 was applied to 0.5 μg/mL LPS-induced RAW 264.7 macrophages in vitro at different concentrations (5, 10, 20, 50 and 100 μg/mL). Levels of nitric oxide (NO), pro-inflammatory cytokines interleukin (IL)-1β (IL-1 β), interleukin (IL)-6 (IL-6), tumor necrosis factor (TNF-α) and anti-inflammatory cytokine IL-10 in the supernatant fraction were measured using enzyme-linked immunosorbent assay (ELISA). Expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were detected via Western blot. The inhibition zones and minimal inhibitory concentration (MIC) values of bacterial strains were in the range of 14-26 mm and 62.5-250 μg/mL, respectively. Moreover, a remarkable activity against several fungal strains was revealed. TdLTP4 (5-100 μg/mL) decreased the production of NO (IC
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Cyclooxygenase 2/metabolism ; Cytokines/metabolism ; Humans ; Lipopolysaccharides ; Macrophages/metabolism ; Mice ; NF-kappa B/metabolism ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/genetics ; Nitric Oxide Synthase Type II/metabolism ; RAW 264.7 Cells ; Triticum
Chemical Substances	Anti-Inflammatory Agents ; Cytokines ; Lipopolysaccharides ; NF-kappa B ; Nitric Oxide (31C4KY9ESH) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cyclooxygenase 2 (EC 1.14.99.1)
Language	English
Publishing date	2021-03-24
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2021.104869
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2136: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito