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  1. Article ; Online: In Memoriam Claudina Rodrigues-Pousada (1941-2021), a dedicated woman in science.

    Bensaude, Olivier

    Cell stress & chaperones

    2021  Volume 26, Issue 3, Page(s) 455–456

    Language English
    Publishing date 2021-04-02
    Publishing country Netherlands
    Document type Editorial
    ZDB-ID 1362749-1
    ISSN 1466-1268 ; 1355-8145
    ISSN (online) 1466-1268
    ISSN 1355-8145
    DOI 10.1007/s12192-021-01202-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4797: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Correction: Isa et al. HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation.

    Isa, Nur Firdaus / Bensaude, Olivier / Aziz, Nadiah C / Murphy, Shona

    Vaccines

    2024  Volume 12, Issue 4

    Abstract: The authors would like to make the following corrections to this published paper [ ... ]. ...

    Abstract The authors would like to make the following corrections to this published paper [...].
    Language English
    Publishing date 2024-03-26
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines12040354
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: HEXIM1 Has Different Functions within Different RNA-Protein Complexes.

    Bensaude, Olivier

    Molecular cell

    2017  Volume 67, Issue 3, Page(s) 357–359

    Abstract: In this issue of Molecular Cell, Morchikh et al. (2017) describe a new ribonuclear complex built around HEXIM1 and the long non-coding RNA NEAT1. This complex regulates the innate immune response to DNA viruses and is distinct from the HEXIM1-7SK RNA ... ...

    Abstract In this issue of Molecular Cell, Morchikh et al. (2017) describe a new ribonuclear complex built around HEXIM1 and the long non-coding RNA NEAT1. This complex regulates the innate immune response to DNA viruses and is distinct from the HEXIM1-7SK RNA complex that regulates transcription elongation.
    MeSH term(s) HeLa Cells ; Humans ; Positive Transcriptional Elongation Factor B/genetics ; RNA, Long Noncoding ; RNA, Small Nuclear/genetics ; RNA-Binding Proteins/genetics
    Chemical Substances RNA, Long Noncoding ; RNA, Small Nuclear ; RNA-Binding Proteins ; Positive Transcriptional Elongation Factor B (EC 2.7.11.-)
    Language English
    Publishing date 2017-08-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2017.07.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5055: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Amber Suppression Technology for Mapping Site-specific Viral-host Protein Interactions in Mammalian Cells.

    Isa, Nur Firdaus / Bensaude, Olivier / Murphy, Shona

    Bio-protocol

    2022  Volume 12, Issue 3, Page(s) e4315

    Abstract: Probing the molecular interactions of viral-host protein complexes to understand pathogenicity is essential in modern virology to help the development of antiviral therapies. Common binding assays, such as co-immunoprecipitation or pull-downs, are ... ...

    Abstract Probing the molecular interactions of viral-host protein complexes to understand pathogenicity is essential in modern virology to help the development of antiviral therapies. Common binding assays, such as co-immunoprecipitation or pull-downs, are helpful in investigating intricate viral-host proteins interactions. However, such assays may miss low-affinity and favour non-specific interactions. We have recently incorporated photoreactive amino acids at defined residues of a viral protein
    Language English
    Publishing date 2022-02-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4315
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: HEXIM1 Has Different Functions within Different RNA-Protein Complexes

    Bensaude, Olivier

    Molecular cell. 2017 Aug. 03, v. 67, no. 3

    2017  

    Abstract: In this issue of Molecular Cell, Morchikh et al. (2017) describe a new ribonuclear complex built around HEXIM1 and the long non-coding RNA NEAT1. This complex regulates the innate immune response to DNA viruses and is distinct from the HEXIM1-7SK RNA ... ...

    Abstract In this issue of Molecular Cell, Morchikh et al. (2017) describe a new ribonuclear complex built around HEXIM1 and the long non-coding RNA NEAT1. This complex regulates the innate immune response to DNA viruses and is distinct from the HEXIM1-7SK RNA complex that regulates transcription elongation.
    Keywords DNA viruses ; innate immunity ; non-coding RNA ; transcription (genetics)
    Language English
    Dates of publication 2017-0803
    Size p. 357-359.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2017.07.021
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2005/501: Show issues

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  6. Article ; Online: Hexim1, an RNA-controlled protein hub.

    Michels, Annemieke A / Bensaude, Olivier

    Transcription

    2018  Volume 9, Issue 4, Page(s) 262–271

    Abstract: Hexim1 acts as a tumor suppressor and is involved in the regulation of innate immunity. It was initially described as a non-coding RNA-dependent regulator of transcription. Here, we detail how 7SK RNA binds to Hexim1 and turns it into an inhibitor of the ...

    Abstract Hexim1 acts as a tumor suppressor and is involved in the regulation of innate immunity. It was initially described as a non-coding RNA-dependent regulator of transcription. Here, we detail how 7SK RNA binds to Hexim1 and turns it into an inhibitor of the positive transcription elongation factor (P-TEFb). In addition to its action on P-TEFb, it plays a role in a variety of different mechanisms: it controls the stability of transcription factor components and assists binding of transcription factors to their targets.
    MeSH term(s) Humans ; Immunity, Innate ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; RNA-Binding Proteins/immunology ; RNA-Binding Proteins/metabolism ; Transcription, Genetic/drug effects ; Transcription, Genetic/genetics
    Chemical Substances HEXIM1 protein, human ; RNA, Long Noncoding ; RNA-Binding Proteins ; long non-coding RNA 7SK, human
    Language English
    Publishing date 2018-02-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2646974-1
    ISSN 2154-1272 ; 2154-1264
    ISSN (online) 2154-1272
    ISSN 2154-1264
    DOI 10.1080/21541264.2018.1429836
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Inhibiting eukaryotic transcription: Which compound to choose? How to evaluate its activity?

    Bensaude, Olivier

    Transcription

    2011  Volume 2, Issue 3, Page(s) 103–108

    Abstract: This review first discusses ways in which we can evaluate transcription inhibition, describe changes in nuclear structure due to transcription inhibition, and report on genes that are paradoxically stimulated by transcription inhibition. Next, it ... ...

    Abstract This review first discusses ways in which we can evaluate transcription inhibition, describe changes in nuclear structure due to transcription inhibition, and report on genes that are paradoxically stimulated by transcription inhibition. Next, it summarizes the characteristics and mechanisms of commonly used inhibitors: α-amanitin is highly selective for RNAP II and RNAP III but its action is slow, actinomycin D is fast but its selectivity is poor, CDK9 inhibitors such as DRB and flavopiridol are fast and reversible but many genes escape transcription inhibition. New compounds, such as triptolide, are fast and selective and able to completely arrest transcription by triggering rapid degradation of RNAP II.
    Language English
    Publishing date 2011-09-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2646974-1
    ISSN 2154-1272 ; 2154-1264
    ISSN (online) 2154-1272
    ISSN 2154-1264
    DOI 10.4161/trns.2.3.16172
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  8. Article: HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation.

    Isa, Nur Firdaus / Bensaude, Olivier / Aziz, Nadiah C / Murphy, Shona

    Vaccines

    2021  Volume 9, Issue 10

    Abstract: The Herpes Simplex Virus (HSV-1) immediate-early protein ICP22 interacts with cellular proteins to inhibit host cell gene expression and promote viral gene expression. ICP22 inhibits phosphorylation of Ser2 of the RNA polymerase II (pol II) carboxyl- ... ...

    Abstract The Herpes Simplex Virus (HSV-1) immediate-early protein ICP22 interacts with cellular proteins to inhibit host cell gene expression and promote viral gene expression. ICP22 inhibits phosphorylation of Ser2 of the RNA polymerase II (pol II) carboxyl-terminal domain (CTD) and productive elongation of pol II. Here we show that ICP22 affects elongation of pol II through both the early-elongation checkpoint and the poly(A)-associated elongation checkpoint of a protein-coding gene model. Coimmunoprecipitation assays using tagged ICP22 expressed in human cells and pulldown assays with recombinant ICP22 in vitro coupled with mass spectrometry identify transcription elongation factors, including P-TEFb, additional CTD kinases and the FACT complex as interacting cellular factors. Using a photoreactive amino acid incorporated into ICP22, we found that L191, Y230 and C225 crosslink to both subunits of the FACT complex in cells. Our findings indicate that ICP22 interacts with critical elongation regulators to inhibit transcription elongation of cellular genes, which may be vital for HSV-1 pathogenesis. We also show that the HSV viral activator, VP16, has a region of structural similarity to the ICP22 region that interacts with elongation factors, suggesting a model where VP16 competes with ICP22 to deliver elongation factors to viral genes.
    Language English
    Publishing date 2021-09-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9101054
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  9. Article ; Online: HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system

    Kolesnikova Olga / Zachayus Amélie / Pichard Simon / Osz Judit / Rochel Natacha / Rossolillo Paola / Kolb-Cheynel Isabelle / Troffer-Charlier Nathalie / Compe Emmanuel / Bensaude Olivier / Berger Imre / Poterszman Arnaud

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Volume 11

    Abstract: Abstract The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette ... ...

    Abstract Abstract The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a rigorous characterization of recombinant bacmids but involves multiple steps, a limitation when many constructs are to be tested. For parallel expression screening and potential high throughput applications, we have established an open source multigene-expression toolbox exploiting homologous recombination, thus reducing the recombinant baculovirus generation to a single-step procedure and shortening the time from cloning to protein production to 2 weeks. The HR-bac toolbox is composed of a set of engineered bacmids expressing a fluorescent marker to monitor virus propagation and a library of transfer vectors. They contain single or dual expression cassettes bearing different affinity tags and their design facilitates the mix and match utilization of expression units from Multibac constructs. The overall cost of virus generation with HR-bac toolbox is relatively low as the preparation of linearized baculoviral DNA only requires standard reagents. Various multiprotein assemblies (nuclear hormone receptor heterodimers, the P-TEFb or the ternary CAK kinase complex associated with the XPD TFIIH subunit) are used as model systems to validate the toolbox presented.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: An alternative D. melanogaster 7SK snRNP.

    Nguyen, Duy / Buisine, Nicolas / Fayol, Olivier / Michels, Annemieke A / Bensaude, Olivier / Price, David H / Uguen, Patricia

    BMC molecular and cell biology

    2021  Volume 22, Issue 1, Page(s) 43

    Abstract: Background: The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are ... ...

    Abstract Background: The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa.
    Results: Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues.
    Conclusion: We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster.
    MeSH term(s) Animals ; Cyclin T/metabolism ; Drosophila Proteins/metabolism ; Drosophila melanogaster ; Positive Transcriptional Elongation Factor B/genetics ; Positive Transcriptional Elongation Factor B/metabolism ; Protein Binding ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; RNA, Small Nuclear/genetics ; RNA, Small Nuclear/metabolism ; RNA-Binding Proteins/metabolism ; Ribonucleoproteins/metabolism ; Ribonucleoproteins, Small Nuclear/metabolism ; Transcription Factors
    Chemical Substances CycT protein, Drosophila ; Cyclin T ; Drosophila Proteins ; HEXIM protein, Drosophila ; LARP7 protein, Drosophila ; RNA, Long Noncoding ; RNA, Small Nuclear ; RNA-Binding Proteins ; Ribonucleoproteins ; Ribonucleoproteins, Small Nuclear ; Transcription Factors ; Positive Transcriptional Elongation Factor B (EC 2.7.11.-)
    Language English
    Publishing date 2021-08-31
    Publishing country England
    Document type Journal Article
    ISSN 2661-8850
    ISSN (online) 2661-8850
    DOI 10.1186/s12860-021-00381-7
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