LIVIVO - Search results -

Search results

Result 1 - 10 of total 35

Search options

Article: Enhanced In Vitro Expression of Filaggrin and Antimicrobial Peptides Following Application of Glycosaminoglycans and a Sphingomyelin-Rich Lipid Extract

Segarra, Sergi / Naiken, Tanesha / Garnier, Julien / Hamon, Valérie / Coussay, Nathalie / Bernard, François-Xavier

Veterinary sciences. 2022 June 27, v. 9, no. 7

2022

Abstract: Filaggrin is an epidermal protein involved in skin barrier formation and hydration, whose expression is altered in canine atopic dermatitis (CAD). CAD patients also present an abnormal immune response with an altered expression of antimicrobial peptides ( ...

Abstract	Filaggrin is an epidermal protein involved in skin barrier formation and hydration, whose expression is altered in canine atopic dermatitis (CAD). CAD patients also present an abnormal immune response with an altered expression of antimicrobial peptides (AMPs), such as β-defensins and cathelicidins. Sphingolipids and glycosaminoglycans (GAGs) have been reported to improve the skin barrier in several animal species, including dogs. Our objective was to evaluate the in vitro effects of a sphingomyelin-rich lipid extract (LE), a hyaluronic acid-rich GAG matrix, and their combination, on the expression of filaggrin and human β-defensin 2 (hBD-2). Filaggrin expression was quantified in a reconstructed human epidermis (RHE), and hBD-2 in normal human epidermal keratinocyte (NHEK) cultures. LE and GAGs were tested at 0.02 mg/mL, with or without adding a cytokine mix. A significant increase in mean hBD-2, compared to the control (99 pg/mL) was achieved with LE (138 pg/mL) and LE+GAGs (165 pg/mL). Filaggrin increased with GAGs (202% ± 83) and LE (193% ± 44) vs. the stimulated control, but this difference was statistically significant (p < 0.05) only with LE+GAGs (210% ± 39). In conclusion, the tested GAGs and LE enhance filaggrin and AMP expression in vitro, which might benefit CAD patients if applied in vivo.
Keywords	artificial skin ; atopic dermatitis ; cathelicidins ; cytokines ; dogs ; glycosaminoglycans ; humans ; immune response ; keratinocytes ; sphingolipids
Language	English
Dates of publication	2022-0627
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2768971-2
ISSN	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9070323
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Enhanced In Vitro Expression of Filaggrin and Antimicrobial Peptides Following Application of Glycosaminoglycans and a Sphingomyelin-Rich Lipid Extract.

Segarra, Sergi / Naiken, Tanesha / Garnier, Julien / Hamon, Valérie / Coussay, Nathalie / Bernard, François-Xavier

Veterinary sciences

2022 Volume 9, Issue 7

Abstract	Filaggrin is an epidermal protein involved in skin barrier formation and hydration, whose expression is altered in canine atopic dermatitis (CAD). CAD patients also present an abnormal immune response with an altered expression of antimicrobial peptides (AMPs), such as β-defensins and cathelicidins. Sphingolipids and glycosaminoglycans (GAGs) have been reported to improve the skin barrier in several animal species, including dogs. Our objective was to evaluate the in vitro effects of a sphingomyelin-rich lipid extract (LE), a hyaluronic acid-rich GAG matrix, and their combination, on the expression of filaggrin and human β-defensin 2 (hBD-2). Filaggrin expression was quantified in a reconstructed human epidermis (RHE), and hBD-2 in normal human epidermal keratinocyte (NHEK) cultures. LE and GAGs were tested at 0.02 mg/mL, with or without adding a cytokine mix. A significant increase in mean hBD-2, compared to the control (99 pg/mL) was achieved with LE (138 pg/mL) and LE+GAGs (165 pg/mL). Filaggrin increased with GAGs (202% ± 83) and LE (193% ± 44) vs. the stimulated control, but this difference was statistically significant (p < 0.05) only with LE+GAGs (210% ± 39). In conclusion, the tested GAGs and LE enhance filaggrin and AMP expression in vitro, which might benefit CAD patients if applied in vivo.
Language	English
Publishing date	2022-06-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9070323
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: IL-34 Exerts Anti-Inflammatory Activities on Keratinocytes and Is Downregulated in Psoriatic-Inflamed Skin.

Croquette, Magali / Faugeroux, Alicia / Fonlupt, Clémence / Godet, Julie / Frouin, Éric / Garcia, Martine / Bernard, François-Xavier / Cordier-Dirikoc, Sevda / Pedretti, Nathalie / Larid, Guillaume / Favot, Laure / Roblot, Pascal / Boutin, Damien / Hainaut-Wierzbicka, Ewa / Heymann, Dominique / Lecron, Jean-Claude / Morel, Franck / Jégou, Jean-François

The Journal of investigative dermatology

2023 Volume 143, Issue 12, Page(s) 2521–2524.e5

MeSH term(s)	Keratinocytes ; Skin ; Interleukins/genetics
Chemical Substances	Interleukins
Language	English
Publishing date	2023-06-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80136-7
ISSN	1523-1747 ; 0022-202X
ISSN (online)	1523-1747
ISSN	0022-202X
DOI	10.1016/j.jid.2023.05.023
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui VI Zs.124: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Dermal fibroblasts are the key sensors of aseptic skin inflammation through interleukin 1 release by lesioned keratinocytes.

Cordier-Dirikoc, Sevda / Pedretti, Nathalie / Garnier, Julien / Clarhaut-Charreau, Sandrine / Ryffel, Bernhard / Morel, Franck / Bernard, François-Xavier / Hamon de Almeida, Valérie / Lecron, Jean-Claude / Jégou, Jean-François

Frontiers in immunology

2022 Volume 13, Page(s) 984045

Abstract: IL-1 plays a crucial role in triggering sterile inflammation following tissue injury. Although most studies associate IL-1 release by injured cells to the recruitment of neutrophils for tissue repair, the inflammatory cascade involves several molecular ... ...

Abstract	IL-1 plays a crucial role in triggering sterile inflammation following tissue injury. Although most studies associate IL-1 release by injured cells to the recruitment of neutrophils for tissue repair, the inflammatory cascade involves several molecular and cellular actors whose role remains to be specified. In the present study, we identified dermal fibroblasts among the IL-1R1-expressing skin cells as key sensors of IL-1 released by injured keratinocytes. After
MeSH term(s)	Mice ; Animals ; Interleukin-1/metabolism ; Interleukin 1 Receptor Antagonist Protein/metabolism ; Interleukin-8/metabolism ; Endothelial Cells/metabolism ; Cells, Cultured ; Keratinocytes/metabolism ; Dermatitis/metabolism ; Fibroblasts/metabolism ; Inflammation/metabolism
Chemical Substances	Interleukin-1 ; Interleukin 1 Receptor Antagonist Protein ; Interleukin-8
Language	English
Publishing date	2022-10-03
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.984045
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: IL-17 and IL-22 are pivotal cytokines to delay wound healing of

Lecron, Jean-Claude / Charreau, Sandrine / Jégou, Jean-François / Salhi, Nadjet / Petit-Paris, Isabelle / Guignouard, Emmanuel / Burucoa, Christophe / Favot-Laforge, Laure / Bodet, Charles / Barra, Anne / Huguier, Vincent / Mcheik, Jiad / Dumoutier, Laure / Garnier, Julien / Bernard, François-Xavier / Ryffel, Bernhard / Morel, Franck

Frontiers in immunology

2022 Volume 13, Page(s) 984016

Abstract: Introduction: Although the presence of pathogens in skin wounds is known to delay the wound healing process, the mechanisms underlying this delay remain poorly understood. In the present study, we have investigated the regulatory role of proinflammatory ...

Abstract	Introduction: Although the presence of pathogens in skin wounds is known to delay the wound healing process, the mechanisms underlying this delay remain poorly understood. In the present study, we have investigated the regulatory role of proinflammatory cytokines on the healing kinetics of infected wounds. Methods: We have developed a mouse model of cutaneous wound healing, with or without wound inoculation with Results: Aseptic excision in C57BL/6 mouse skin induced early expression of IL-1β, TNFα and Oncostatin M (OSM), without detectable expression of IL-22 and IL-17A/F. Conclusion: These results demonstrate the synergistic and specific effects of IL-22 and IL-17 induced by bacterial infection delay the wound healing process, regardless of the presence of bacteria
MeSH term(s)	Mice ; Humans ; Animals ; Pseudomonas aeruginosa/metabolism ; Interleukin-17/metabolism ; Staphylococcus aureus/metabolism ; Tumor Necrosis Factor-alpha ; Oncostatin M ; Methicillin-Resistant Staphylococcus aureus/metabolism ; Mice, Inbred C57BL ; Interleukin-22
Chemical Substances	Interleukin-17 ; Tumor Necrosis Factor-alpha ; Oncostatin M (106956-32-5)
Language	English
Publishing date	2022-10-07
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.984016
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective.

Mcheik, Jiad N / Barrault, Christine / Levard, Guillaume / Morel, Franck / Bernard, François-Xavier / Lecron, Jean-Claude

Plastic and reconstructive surgery. Global open

2014 Volume 2, Issue 9, Page(s) e218

Abstract: Background: Treatment of burned patients is a tricky clinical problem not only because of the extent of the physiologic abnormalities but also because of the limited area of normal skin available.: Methods: Literature indexed in the National Center ( ... ...

Abstract	Background: Treatment of burned patients is a tricky clinical problem not only because of the extent of the physiologic abnormalities but also because of the limited area of normal skin available. Methods: Literature indexed in the National Center (PubMed) has been reviewed using combinations of key words (burns, children, skin graft, tissue engineering, and keratinocyte grafts). Articles investigating the association between burns and graft therapeutic modalities have been considered. Further literature has been obtained by analysis of references listed in reviewed articles. Results: Severe burns are conventionally treated with split-thickness skin autografts. However, there are usually not enough skin donor sites. For years, the question of how covering the wound surface became one of the major challenges in clinical research area and several procedures were proposed. The microskin graft is one of the oldest methods to cover extensive burns. This technique of skin expansion is efficient, but results remain inconsistent. An alternative is to graft cultured human epidermal keratinocytes. However, because of several complications and labor-intensive process of preparing grafts, the initial optimism for cultured epithelial autograft has gradually declined. In an effort to solve these drawbacks, isolated epithelial cells from selecting donor site were introduced in skin transplantation. Conclusions: Cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.
Language	English
Publishing date	2014-10-07
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000000176
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 7091: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo.

Boukhedouni, Nesrine / Martins, Christina / Darrigade, Anne-Sophie / Drullion, Claire / Rambert, Jérôme / Barrault, Christine / Garnier, Julien / Jacquemin, Clément / Thiolat, Denis / Lucchese, Fabienne / Morel, Franck / Ezzedine, Khaled / Taieb, Alain / Bernard, François-Xavier / Seneschal, Julien / Boniface, Katia

JCI insight

2020 Volume 5, Issue 11

Abstract: Loss of melanocytes is the pathological hallmark of vitiligo, a chronic inflammatory skin depigmenting disorder induced by exaggerated immune response, including autoreactive CD8 T cells producing high levels of type 1 cytokines. However, the interplay ... ...

Abstract	Loss of melanocytes is the pathological hallmark of vitiligo, a chronic inflammatory skin depigmenting disorder induced by exaggerated immune response, including autoreactive CD8 T cells producing high levels of type 1 cytokines. However, the interplay between this inflammatory response and melanocyte disappearance remains to be fully characterized. Here, we demonstrate that vitiligo skin contains a significant proportion of suprabasal melanocytes, associated with disruption of E-cadherin expression, a major protein involved in melanocyte adhesion. This phenomenon is also observed in lesional psoriatic skin. Importantly, apoptotic melanocytes were mainly observed once cells were detached from the basal layer of the epidermis, suggesting that additional mechanism(s) could be involved in melanocyte loss. The type 1 cytokines IFN-γ and TNF-α induce melanocyte detachment through E-cadherin disruption and the release of its soluble form, partly due to MMP-9. The levels of MMP-9 are increased in the skin and sera of patients with vitiligo, and MMP-9 is produced by keratinocytes in response to IFN-γ and TNF-α. Inhibition of MMP-9 or the JAK/STAT signaling pathway prevents melanocyte detachment in vitro and in vivo. Therefore, stabilization of melanocytes in the basal layer of the epidermis by preventing E-cadherin disruption appears promising for the prevention of depigmentation occurring in vitiligo and during chronic skin inflammation.
MeSH term(s)	Animals ; Cadherins/metabolism ; Humans ; Interferon-gamma/metabolism ; Keratinocytes/metabolism ; Keratinocytes/pathology ; MAP Kinase Signaling System ; Matrix Metalloproteinase 9/biosynthesis ; Melanocytes/metabolism ; Melanocytes/pathology ; Mice ; Tumor Necrosis Factor-alpha/metabolism ; Vitiligo/metabolism
Chemical Substances	Cadherins ; Cdh1 protein, mouse ; IFNG protein, human ; IFNG protein, mouse ; Tumor Necrosis Factor-alpha ; Interferon-gamma (82115-62-6) ; MMP9 protein, human (EC 3.4.24.35) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Mmp9 protein, mouse (EC 3.4.24.35)
Language	English
Publishing date	2020-06-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.133772
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts.

Mcheik, Jiad N / Barrault, Christine / Pedretti, Nathalie / Garnier, Julien / Juchaux, Franck / Levard, Guillaume / Morel, Franck / Lecron, Jean-Claude / Bernard, François-Xavier

Journal of tissue engineering and regenerative medicine

2016 Volume 10, Issue 3, Page(s) 252–260

Abstract: Severe burns in children are conventionally treated with split-thickness skin autografts or epidermal sheets. However, neither early complete healing nor quality of epithelialization is satisfactory. An alternative approach is to graft isolated ... ...

Abstract	Severe burns in children are conventionally treated with split-thickness skin autografts or epidermal sheets. However, neither early complete healing nor quality of epithelialization is satisfactory. An alternative approach is to graft isolated keratinocytes. We evaluated paediatric foreskin and auricular skin as donor sources, autologous keratinocyte transplantation, and compared the graft efficiency to the in vitro capacities of isolated keratinocytes to divide and reconstitute epidermal tissue. Keratinocytes were isolated from surgical samples by enzymatic digestion. Living cell recovery, in vitro proliferation and epidermal reconstruction capacities were evaluated. Differentiation status was analysed, using qRT-PCR and immunolabelling. Eleven children were grafted with foreskin-derived (boys) or auricular (girls) keratinocyte suspensions dripped onto deep severe burns. The aesthetic and functional quality of epithelialization was monitored in a standardized way. Foreskin keratinocyte graft in male children provides for the re-epithelialization of partial deep severe burns and accelerates wound healing, thus allowing successful wound closure, and improves the quality of scars. In accordance, in vitro studies have revealed a high yield of living keratinocyte recovery from foreskin and their potential in terms of regeneration and differentiation. We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. In vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.
MeSH term(s)	Biomarkers/metabolism ; Burns/pathology ; Cell Differentiation ; Cell Separation/methods ; Cells, Cultured ; Child ; Child, Preschool ; Ear ; Epidermis/cytology ; Foreskin/cytology ; Humans ; Immunohistochemistry ; Infant ; Keratinocytes/cytology ; Male ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Regeneration ; Skin Transplantation ; Stem Cells/cytology ; Transplantation, Autologous ; Wound Healing
Chemical Substances	Biomarkers ; RNA, Messenger
Language	English
Publishing date	2016-03
Publishing country	England
Document type	Journal Article
ISSN	1932-7005
ISSN (online)	1932-7005
DOI	10.1002/term.1690
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Pseudomonas aeruginosa flagellum is critical for invasion, cutaneous persistence and induction of inflammatory response of skin epidermis.

Garcia, Magali / Morello, Eric / Garnier, Julien / Barrault, Christine / Garnier, Martine / Burucoa, Christophe / Lecron, Jean-Claude / Si-Tahar, Mustapha / Bernard, François-Xavier / Bodet, Charles

Virulence

2018 Volume 9, Issue 1, Page(s) 1163–1175

Abstract: Pseudomonas aeruginosa, an opportunistic pathogen involved in skin and lung diseases, possesses numerous virulence factors, including type 2 and 3 secretion systems (T2SS and T3SS) and its flagellum, whose functions remain poorly known during cutaneous ... ...

Abstract	Pseudomonas aeruginosa, an opportunistic pathogen involved in skin and lung diseases, possesses numerous virulence factors, including type 2 and 3 secretion systems (T2SS and T3SS) and its flagellum, whose functions remain poorly known during cutaneous infection. Using isogenic mutants deleted from genes encoding each or all of these three virulence factors, we investigated their role in induction of inflammatory response and in tissue invasiveness in human primary keratinocytes and reconstructed epidermis. Our results showed that flagellum, but not T2SS and T3SS, is involved in induction of a large panel of cytokine, chemokine, and antimicrobial peptide (AMP) mRNA in the infected keratinocytes. Chemokine secretion and AMP tissular production were also dependent on the presence of the bacterial flagellum. This pro-inflammatory effect was significantly reduced in keratinocytes infected in presence of anti-toll-like receptor 5 (TLR5) neutralizing antibody. Bacterial invasion of human epidermis and persistence in a mouse model of sub-cutaneous infection were dependent on the P. aeruginosa flagellum. We demonstrated that flagellum constitutes the main virulence factor of P. aeruginosa involved not only in early induction of the epidermis inflammatory response but also in bacterial invasion and cutaneous persistence. P. aeruginosa is mainly sensed by TLR5 during the early innate immune response of human primary keratinocytes.
MeSH term(s)	Animals ; Antibodies, Neutralizing/pharmacology ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/immunology ; Cells, Cultured ; Chemokines/genetics ; Chemokines/immunology ; Cytokines/genetics ; Cytokines/immunology ; Disease Models, Animal ; Epidermis/microbiology ; Flagella/physiology ; Humans ; Immunity, Innate ; Inflammation/microbiology ; Keratinocytes/drug effects ; Keratinocytes/immunology ; Keratinocytes/microbiology ; Male ; Mice ; Mutation ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/immunology ; Pseudomonas aeruginosa/pathogenicity ; Pseudomonas aeruginosa/ultrastructure ; Toll-Like Receptor 5/immunology ; Virulence Factors/deficiency ; Virulence Factors/genetics
Chemical Substances	Antibodies, Neutralizing ; Antimicrobial Cationic Peptides ; Chemokines ; Cytokines ; TLR5 protein, human ; Toll-Like Receptor 5 ; Virulence Factors
Language	English
Publishing date	2018-08-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2657572-3
ISSN	2150-5608 ; 2150-5594
ISSN (online)	2150-5608
ISSN	2150-5594
DOI	10.1080/21505594.2018.1480830
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor.

Barrault, Christine / Garnier, Julien / Pedretti, Nathalie / Cordier-Dirikoc, Sevda / Ratineau, Emeline / Deguercy, Alain / Bernard, François-Xavier

The Journal of steroid biochemistry and molecular biology

2015 Volume 152, Page(s) 34–44

Abstract: Androgens act through non-genomic and androgen receptor (AR)-dependent genomic mechanisms. AR is expressed in the sebaceous gland and the importance of androgens in the sebaceous function is well established. However, the in vitro models used to date ... ...

Abstract	Androgens act through non-genomic and androgen receptor (AR)-dependent genomic mechanisms. AR is expressed in the sebaceous gland and the importance of androgens in the sebaceous function is well established. However, the in vitro models used to date have failed to evidence a clear genomic effect (e.g., modification of gene expression profile) of androgens on human sebocyte cells. In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues. Moreover, we observed that DHT precociously down-regulated markers for immature follicular cells (KRT15, TNC) and for hair lineage (KRT75, FST) and up-regulated the expression of genes potentially related to sebocyte differentiation (MUC1/EMA, AQP3, FADS2). These effects were fully confirmed at the protein level. In addition, DHT-stimulated SEBO662 AR+, cultured in a low-calcium defined keratinocyte medium without serum or any complement, neosynthesize lipids, including sebum lipids, and store increased amounts of triglycerides in lipid droplets. DHT also induces morphological changes, increases cell size, and treatments over 7 days lead to a time-dependent increase in the population of apoptotic DNA-fragmented cells. Taken together, these results show for the first time that active androgens alone can engage immature sebocytes in a clear lipogenic differentiation process (Graphical abstract). These effects depend on the expression of a functional AR in these cells. This model should be of interest for revisiting the mechanisms of the sebaceous function in vitro and for the design of relevant pharmacological models for drug or compound testing.
MeSH term(s)	Active Transport, Cell Nucleus ; Androgens/metabolism ; Apoptosis ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dihydrotestosterone/metabolism ; Hair Follicle/cytology ; Humans ; Keratinocytes/cytology ; Receptors, Androgen/biosynthesis ; Sebaceous Glands/cytology ; Sebaceous Glands/metabolism
Chemical Substances	AR protein, human ; Androgens ; Receptors, Androgen ; Dihydrotestosterone (08J2K08A3Y)
Language	English
Publishing date	2015-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1049188-0
ISSN	1879-1220 ; 0960-0760
ISSN (online)	1879-1220
ISSN	0960-0760
DOI	10.1016/j.jsbmb.2015.04.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 708: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito