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Article: Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus.

Caruso, Andrea Giovanni / Ragona, Arianna / Bertacca, Sofia / Montoya, Mauricio Alejandro Marin / Panno, Stefano / Davino, Salvatore

Plants (Basel, Switzerland)

2023 Volume 12, Issue 7

Abstract: Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample ... ...

Abstract	Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions on zucchini squash, tomato, and pepper samples. A set of six LAMP primers was designed for specific ToCLNDV detection, targeting a 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity and accuracy of the real-time LAMP assay and comparison with canonical PCR were evaluated. The real-time LAMP assay developed was about one-thousand times more sensitive than the conventional PCR method, detecting a total of 4.41 × 10
Language	English
Publishing date	2023-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12071487
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)

Caruso, Andrea Giovanni / Bertacca, Sofia / Ragona, Arianna / Matić, Slavica / Davino, Salvatore / Panno, Stefano

Agriculture (Basel). 2022 June 09, v. 12, no. 6

2022

Abstract: Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by ...

Abstract	Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GVA detection were conducted and subsequently confirmed by molecular (RT-PCR) analysis. Results showed that 10 out of the 11 cultivars analyzed were positive to GVA, for a total of 49 out of 617 samples (8%). A higher incidence of infection was detected on ‘Nerello Mascalese’, ‘Carricante’, ‘Perricone’ and ‘Nero d’Avola’ cultivars, followed by ‘Alicante’, ‘Grecanico’, ‘Catarratto’, ‘Grillo’, ‘Nerello Cappuccio’ and ‘Zibibbo’, while in the ‘Moscato’ cultivar no infection was found. Phylogenetic analyses carried out on the coat protein (CP) gene of 16 GVA sequences selected in this study showed a low variability degree among the Sicilian isolates, closely related with other Italian isolates retrieved in GenBank, suggesting a common origin, probably due to the exchange of infected propagation material within the Italian territory.
Keywords	Grapevine virus A ; Vitis ; agriculture ; coat proteins ; cultivars ; enzyme-linked immunosorbent assay ; genes ; phylogeny ; propagation materials ; scale insects ; viruses ; Italy ; Sicily
Language	English
Dates of publication	2022-0609
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture12060835
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Tomato brown rugose fruit virus: A pathogen that is changing the tomato production worldwide

Caruso, Andrea Giovanni / Bertacca, Sofia / Parrella, Giuseppe / Rizzo, Roberto / Davino, Salvatore / Panno, Stefano

Annals of Applied Biology. 2022 Nov., v. 181, no. 3 p.258-274

2022

Abstract: Tomato (Solanum lycopersicum L., family Solanaceae) represents one of the most cultivated horticultural crops worldwide, with over 5 million hectares of cultivated area and more than 182 million tons of tomato produced globally. Nevertheless, monoculture ...

Abstract	Tomato (Solanum lycopersicum L., family Solanaceae) represents one of the most cultivated horticultural crops worldwide, with over 5 million hectares of cultivated area and more than 182 million tons of tomato produced globally. Nevertheless, monoculture conditions, intensive selection, domestication throughout the last decades, international trade of infected propagating material and climate changes intensely favoured the establishment of many pathogens and the rapid spread of new diseases, allowing organisms to establish in new and unfavourable environments. Among different biotic agents, viruses are the most dangerous, because of their rapid diffusion and production losses. Here, we review an emerging viral threat to tomato production, tomato brown rugose fruit virus (ToBRFV), a new highly infectious tobamovirus that is currently causing great concern to tomato global production, especially in those areas where mitigation measures are absent or inadequate and which, in recent years, it has considerably increased its diffusion in new tomato cultivation areas. Through a review of all the existing literature, this article highlights the following aspects: (a) main characteristic of tomato species (origin, taxonomy and genome); (b) main diseases that undermine the tomato production, focusing on viral pathogens; (c) ToBRFV main characteristics (origin and spatiotemporal dispersal, taxonomy, genome organisation, host range and symptoms, transmission, spread and epidemiology, and genetic diversity); (d) detection methods developed and disease management; (e) breeding as a new weapon to control the ToBRFV diffusion. Moreover, future perspectives are highlighted, to understand the epidemiology key factors and the ToBRFV‐tomato pathosystem management, in order to develop effective and appropriate control strategies.
Keywords	Solanum lycopersicum ; Tobamovirus ; climate ; cultivation area ; disease control ; domestication ; epidemiology ; fruits ; genetic variation ; genome ; horticulture ; host range ; international trade ; pathogens ; taxonomy ; tomatoes ; viruses
Language	English
Dates of publication	2022-11
Size	p. 258-274.
Publishing place	Blackwell Publishing Ltd
Document type	Article ; Online
Note	REVIEW
ZDB-ID	283072-3
ISSN	0003-4746 ; 0951-4309
ISSN	0003-4746 ; 0951-4309
DOI	10.1111/aab.12788
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Detection by Sensitive Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification of Olive Leaf Yellowing Associated Virus and Its Incidence in Italy and Spain

Caruso, Andrea Giovanni / Bertacca, Sofia / Ragona, Arianna / Agrò, Graziella / Font-San-Ambrosio, Maria Isabel / Alfaro-Fernández, Ana / Sánchez, Rocío Estévez / Panno, Stefano / Davino, Salvatore

Horticulturae. 2023 June 15, v. 9, no. 6

2023

Abstract: Olive trees (Olea europea L.) are constantly threatened by many viruses, such as the olive leaf yellowing-associated virus (OLYaV), that belong to the Olivavirus genus, family Closteroviridae. In this work, the OLYaV incidence in different regions of ... ...

Abstract	Olive trees (Olea europea L.) are constantly threatened by many viruses, such as the olive leaf yellowing-associated virus (OLYaV), that belong to the Olivavirus genus, family Closteroviridae. In this work, the OLYaV incidence in different regions of Italy and Spain, which represent the two most important European areas for olive production, was evaluated through the development of a real-time reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for reliable and sensitive OLYaV detection. The specificity and accuracy of the developed real-time RT-LAMP assay were determined; the assay showed that potential cross-reactivity with other viruses belonging to the Closteroviridae family was excluded. The LAMP assay detected OLYaV with a higher sensitivity than conventional end-point RT-PCR, detecting a total of 1.34 × 10⁻² genome copies. A total of 80 and 120 plants of different olive cultivars from Spain (Comunitat Valenciana, Andalusia) and Italy (Sicily, Calabria, Apulia, Lazio, and Umbria) regions were tested, respectively. The percentage of infected plants was 46.25% and 30% for Spain and Italy, respectively, while the most susceptible cultivars were “Serrana Espadán” and “Villalonga” from Comunitat Valenciana and Andalusia regions (Spain) and “Ogliarola barese” from Apulia region (Italy). In addition, the survey demonstrated that the real-time RT-LAMP showed good sensitivity for OLYaV-positive sample detection, especially on asymptomatic olive trees. For this reason, the developed assay could be very suitable for phytopathological laboratories as a reliable and efficient method for a rapid and sensitive routine test on olive samples.
Keywords	Closteroviridae ; Olea europaea ; cross reaction ; cultivars ; genome ; leaves ; olives ; reverse transcription loop-mediated isothermal amplification ; surveys ; viruses ; Italy ; Sicily ; Spain
Language	English
Dates of publication	2023-0615
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2813983-5
ISSN	2311-7524
ISSN	2311-7524
DOI	10.3390/horticulturae9060702
Database	NAL-Catalogue (AGRICOLA)

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Article: Detection of Parietaria Mottle Virus by RT-qPCR: An Emerging Virus Native of Mediterranean Area That Undermine Tomato and Pepper Production in Southern Italy.

Panno, Stefano / Caruso, Andrea Giovanni / Bertacca, Sofia / Matić, Slavica / Davino, Salvatore / Parrella, Giuseppe

Frontiers in plant science

2021 Volume 12, Page(s) 698573

Abstract: Parietaria mottle virus (PMoV) is considered an emerging virus in many countries of the Mediterranean basin, especially on tomato and pepper crops. Symptoms on tomato leaves and fruits can be easily confused with those induced by cucumber mosaic virus ( ... ...

Abstract	Parietaria mottle virus (PMoV) is considered an emerging virus in many countries of the Mediterranean basin, especially on tomato and pepper crops. Symptoms on tomato leaves and fruits can be easily confused with those induced by cucumber mosaic virus (CMV) with necrogenic satellite RNA (CMV-satRNA), tomato spotted wilt virus (TSWV) or tomato mosaic virus (ToMV). Mixed infection of these viruses has been also reported in some tomato cultivars, with an increase in the complexity of the symptoms and severity of the disease. Although a specific serum and riboprobes have been produced, nowadays no sensitive diagnostic methods are available for the rapid PMoV detection. Here, we have developed a RT-qPCR assay with the aim to establish a more sensitive and specific method for PMoV detection. Specific primers and TaqMan probe were designed and
Language	English
Publishing date	2021-09-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2021.698573
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Tomato Brown Rugose Fruit Virus: Seed Transmission Rate and Efficacy of Different Seed Disinfection Treatments

Davino, Salvatore / Caruso, Andrea Giovanni / Bertacca, Sofia / Barone, Stefano / Panno, Stefano

Plants. 2020 Nov. 20, v. 9, no. 11

2020

Abstract: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of ... ...

Abstract	Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of disinfection, and the effects of different thermal- and chemical-based treatments on ToBRFV-infected seeds’ germination. Analyses demonstrated that ToBRFV was located in the seed coat, sometime in the endosperm, but never in the embryo; its transmission from infected seeds to plantlets occurs by micro-lesions during the germination. The ToBRFV seed transmission rate was 2.8% in cotyledons and 1.8% in the third true leaf. Regarding the different disinfection treatments, they returned 100% of germination at 14 days post-treatment (dpt), except for the treatment with 2% hydrochloric acid +1.5% sodium hypochlorite for 24 h, for which no seed germinated after 14 dpt. All treatments have the ability to inactivate ToBRFV, but in six out of seven treatments ToBRFV was still detectable by RT-qPCR. These results raise many questions about the correct way to carry out diagnosis at customs. To our knowledge, this is the first study on the effective localization of ToBRFV particles in seeds.
Keywords	Solanum lycopersicum ; cotyledons ; crop production ; disinfection ; endosperm ; fruits ; germination ; hydrochloric acid ; leaves ; plant viruses ; plantlets ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; seed coat ; sodium hypochlorite ; tomatoes
Language	English
Dates of publication	2020-1120
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants9111615
Database	NAL-Catalogue (AGRICOLA)

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Article: A Review of the Most Common and Economically Important Diseases That Undermine the Cultivation of Tomato Crop in the Mediterranean Basin

Panno, Stefano / Davino, Salvatore / Caruso, Andrea Giovanni / Bertacca, Sofia / Crnogorac, Ana / Mandić, Ana / Noris, Emanuela / Matić, Slavica

Agronomy. 2021 Oct. 29, v. 11, no. 11

2021

Abstract: Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years one of the most important and extensively grown horticultural crops in the Mediterranean region and throughout the world. In 2019, more than 180 million tonnes of ... ...

Abstract	Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years one of the most important and extensively grown horticultural crops in the Mediterranean region and throughout the world. In 2019, more than 180 million tonnes of tomato have been produced worldwide, out of which around 42 million tonnes in Mediterranean countries. Due to its genetic properties, tomato is afflicted by numerous plant diseases induced by fungal, bacterial, phytoplasma, virus, and viroid pathogens. Not only is its genetic inheritance of great importance to the management of the numerous tomato pathogens, but equally as important are also the present climate changes, the recently revised phytopathological control measures, and the globalization of the seed industry. Thus, the recognition of symptoms and the knowledge of the distribution and spread of the disease and of the methods for early detection of the pathogens are the major prerequisites for a successful management of the disease. In this review, we will describe the main tomato pathogens in the Mediterranean area that impact mostly the tomato yield and provide the current and perspective measures necessary for their successful management.
Keywords	Phytoplasma ; Solanum lycopersicum ; agronomy ; climate ; disease transmission ; fungi ; globalization ; horticulture ; inheritance (genetics) ; seed industry ; tomatoes ; viruses ; Mediterranean region
Language	English
Dates of publication	2021-1029
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2607043-1
ISSN	2073-4395
ISSN	2073-4395
DOI	10.3390/agronomy11112188
Database	NAL-Catalogue (AGRICOLA)

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Article: Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily

Panno, Stefano / Caruso, Andrea Giovanni / Bertacca, Sofia / Pisciotta, Antonino / Lorenzo, Rosario Di / Marchione, Serafino / Matić, Slavica / Davino, Salvatore

Agriculture (Basel). 2021 May 27, v. 11, no. 6

2021

Abstract: Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different ... ...

Abstract	Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the samples analyzed, while 48 out of 617 samples gave positive results to GFLV, for a total of 9 out of 11 cultivars analyzed. Phylogenetic analyses carried out on the GFLV-CP gene of 18 Sicilian GFLV sequences selected in this study showed a certain degree of variability among the Sicilian isolates, suggesting a different origin, probably as a consequence of the continuous interchange of GFLV-infected propagating material with other Italian regions or viticultural areas located in other countries.
Keywords	Arabis mosaic virus ; Grapevine fanleaf virus ; Vitis ; agriculture ; cultivars ; enzyme-linked immunosorbent assay ; genes ; phylogeny ; viticulture ; Italy ; Sicily
Language	English
Dates of publication	2021-0527
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture11060496
Database	NAL-Catalogue (AGRICOLA)

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Article: Development of a Real-Time Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Olea Europaea Geminivirus

Bertacca, Sofia / Caruso, Andrea Giovanni / Trippa, Daniela / Marchese, Annalisa / Giovino, Antonio / Matic, Slavica / Noris, Emanuela / Ambrosio, Maria Isabel Font San / Alfaro, Ana / Panno, Stefano / Davino, Salvatore

Plants. 2022 Feb. 28, v. 11, no. 5

2022

Abstract: A real-time loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of the Olea europaea geminivirus (OEGV), a virus recently reported in different olive cultivation areas worldwide. A preliminary ... ...

Abstract	A real-time loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of the Olea europaea geminivirus (OEGV), a virus recently reported in different olive cultivation areas worldwide. A preliminary screening by end-point PCR for OEGV detection was conducted to ascertain the presence of OEGV in Sicily. A set of six real-time LAMP primers, targeting a 209-nucleotide sequence elapsing the region encoding the coat protein (AV1) gene of OEGV, was designed for specific OEGV detection. The specificity, sensitivity, and accuracy of the diagnostic assay were determined. The LAMP assay showed no cross-reactivity with other geminiviruses and was allowed to detect OEGV with a 10-fold higher sensitivity than conventional end-point PCR. To enhance the potential of the LAMP assay for field diagnosis, a simplified sample preparation procedure was set up and used to monitor OEGV spread in different olive cultivars in Sicily. As a result of this survey, we observed that 30 out of 70 cultivars analyzed were positive to OEGV, demonstrating a relatively high OEGV incidence. The real-time LAMP assay developed in this study is suitable for phytopathological laboratories with limited facilities and resources, as well as for direct OEGV detection in the field, representing a reliable method for rapid screening of olive plant material.
Keywords	Geminiviridae ; Olea europaea ; coat proteins ; cross reaction ; cultivars ; genes ; loop-mediated isothermal amplification ; olives ; rapid methods ; surveys ; viruses ; Sicily
Language	English
Dates of publication	2022-0228
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11050660
Database	NAL-Catalogue (AGRICOLA)

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Article: Tomato Brown Rugose Fruit Virus

Davino, Salvatore / Caruso, Andrea Giovanni / Bertacca, Sofia / Barone, Stefano / Panno, Stefano

Plants (Basel, Switzerland)

2020 Volume 9, Issue 11

Abstract	Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of disinfection, and the effects of different thermal- and chemical-based treatments on ToBRFV-infected seeds' germination. Analyses demonstrated that ToBRFV was located in the seed coat, sometime in the endosperm, but never in the embryo; its transmission from infected seeds to plantlets occurs by micro-lesions during the germination. The ToBRFV seed transmission rate was 2.8% in cotyledons and 1.8% in the third true leaf. Regarding the different disinfection treatments, they returned 100% of germination at 14 days post-treatment (dpt), except for the treatment with 2% hydrochloric acid +1.5% sodium hypochlorite for 24 h, for which no seed germinated after 14 dpt. All treatments have the ability to inactivate ToBRFV, but in six out of seven treatments ToBRFV was still detectable by RT-qPCR. These results raise many questions about the correct way to carry out diagnosis at customs. To our knowledge, this is the first study on the effective localization of ToBRFV particles in seeds.
Language	English
Publishing date	2020-11-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants9111615
Database	MEDical Literature Analysis and Retrieval System OnLINE

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