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  1. Article: Development of an ecotoxicological test procedure for soil microalgae

    Carvalhido, Vânia / Bessa da Silva, Márcia / Santos, Marina / Tamagnini, Paula / Melo, Paula / Pereira, Ruth

    Science of the total environment. 2021 Aug. 20, v. 783

    2021  

    Abstract: Since the 80s, ISO and OECD organizations have been developing guidelines for assessing the toxicity of new and existing chemical substances to soil biota. Up to now, any of these guidelines had soil algae as test organisms. Nevertheless, microalgae are ... ...

    Abstract Since the 80s, ISO and OECD organizations have been developing guidelines for assessing the toxicity of new and existing chemical substances to soil biota. Up to now, any of these guidelines had soil algae as test organisms. Nevertheless, microalgae are relevant components of soil microbial communities and soil biological crusts (BSC) with a great contribution to different soil functions and ecosystem services. In an attempt to bridge the gap, the present work aimed to develop, describe and validate a standard operating procedure for an ecotoxicological test with soil microalgae. Three phases were performed, each one with specific objectives. First, soil microalgae and cyanobacteria were isolated from BSC and then genetically and morphologically characterized. The green microalga Micractinium inermum was selected because it is a species with a wide geographic distribution. Secondly, M. inermum growth curves were obtained in liquid (BG₁₁ and Woods-Hole MBL) and solid media (OECD artificial soil) to determine test duration. The growth curves were also used to analyze the reproducibility of the test's endpoint and to propose a validation criterion. Ultimately, a range of concentrations of two reference substances (glyphosate and copper) were tested, both in soil and liquid media, to assess procedure's reproducibility. The tests made in liquid medium followed the standard guideline for ecotoxicological tests with freshwater microalgae and cyanobacteria (OECD 201:2011). The results obtained prove that when the artificial soil is used, as a test substrate, the sensitivity of M. inermum increases. The tests performed with both reference substances demonstrate that the procedure described for testing in soil was reproducible. Additionally, it will be relevant to test with other reference substances and adjust the procedure for natural soils. It will be also interesting to validate the test procedure with soil cyanobacteria.
    Keywords Cyanobacteria ; Micractinium ; copper ; ecosystems ; ecotoxicology ; environment ; freshwater ; geographical distribution ; glyphosate ; guidelines ; liquids ; microalgae ; soil biota ; toxicity
    Language English
    Dates of publication 2021-0820
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2021.147006
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Development of an ecotoxicological test procedure for soil microalgae.

    Carvalhido, Vânia / Bessa da Silva, Márcia / Santos, Marina / Tamagnini, Paula / Melo, Paula / Pereira, Ruth

    The Science of the total environment

    2021  Volume 783, Page(s) 147006

    Abstract: Since the 80s, ISO and OECD organizations have been developing guidelines for assessing the toxicity of new and existing chemical substances to soil biota. Up to now, any of these guidelines had soil algae as test organisms. Nevertheless, microalgae are ... ...

    Abstract Since the 80s, ISO and OECD organizations have been developing guidelines for assessing the toxicity of new and existing chemical substances to soil biota. Up to now, any of these guidelines had soil algae as test organisms. Nevertheless, microalgae are relevant components of soil microbial communities and soil biological crusts (BSC) with a great contribution to different soil functions and ecosystem services. In an attempt to bridge the gap, the present work aimed to develop, describe and validate a standard operating procedure for an ecotoxicological test with soil microalgae. Three phases were performed, each one with specific objectives. First, soil microalgae and cyanobacteria were isolated from BSC and then genetically and morphologically characterized. The green microalga Micractinium inermum was selected because it is a species with a wide geographic distribution. Secondly, M. inermum growth curves were obtained in liquid (BG
    MeSH term(s) Ecosystem ; Ecotoxicology ; Microalgae ; Reproducibility of Results ; Soil
    Chemical Substances Soil
    Language English
    Publishing date 2021-04-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2021.147006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: TiO2 nanoparticles for the remediation of eutrophic shallow freshwater systems: Efficiency and impacts on aquatic biota under a microcosm experiment

    Bessa da Silva, Márcia / Fernando Gonçalves / Nelson Abrantes / Ruth Pereira / Verónica Nogueira

    Aquatic toxicology. 2016 Sept., v. 178

    2016  

    Abstract: The application of nanomaterials (NMs) in the remediation of eutrophic waters, particularly in the control of internal loading of nutrients, has been started, but limited investigations evaluated the effectiveness of these new treatment approaches and of ...

    Abstract The application of nanomaterials (NMs) in the remediation of eutrophic waters, particularly in the control of internal loading of nutrients, has been started, but limited investigations evaluated the effectiveness of these new treatment approaches and of their potential impacts on species from shallow freshwater lakes. The present work investigated, under a microcosm experiment, the application of a TiO2 nanomaterial both for reducing nutrient (mainly phosphorus and nitrogen forms) desorption and release from sediments (preventive treatment⿿PT) and for eliminating algal blooms (remediation treatment⿿RT). Furthermore, we also intended to assess the potential impacts of nano-TiO2 application on key freshwater species.The results showed the effectiveness of nano-TiO2 in controlling the release of phosphates from surface sediment and the subsequent reduction of total phosphorus in the water column. A reduction in total nitrogen was also observed. Such changes in nutrient dynamics contributed to a progressive inhibition of development of algae after the application of the NM in PT microcosms. Concerning the ability of nano-TiO2 to interact with algal cells, this interaction has likely occurred, mainly in RT, enhancing the formation of aggregates and their rapid settlement, thus reducing the algal bloom. Both treatments caused deleterious effects on freshwater species. In PT, Daphnia magna and Lemna minor showed a significant inhibition of several endpoints. Conversely, no inhibitory effect on the growth of Chironomus riparius was recorded. In opposite, C. riparius was the most affected species in RT microcosms. Such difference was probably caused by the formation of larger TiO2-algae aggregates in RT, under a high algal density, that rapidly settled in the sediment, becoming less available for pelagic species. In summary, despite the effectiveness of both treatments in controlling internal nutrient loading and in the mitigating algal bloom episodes, their negative effects on biota have to be seriously taken into account.
    Keywords adverse effects ; algae ; algal blooms ; Chironomus riparius ; Daphnia magna ; desorption ; eutrophication ; freshwater ; lakes ; Lemna minor ; nanoparticles ; nitrogen ; nitrogen content ; nutrients ; phosphates ; phosphorus ; pollution load ; remediation ; sediments ; titanium dioxide
    Language English
    Dates of publication 2016-09
    Size p. 58-71.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 782699-0
    ISSN 1879-1514 ; 0166-445X
    ISSN (online) 1879-1514
    ISSN 0166-445X
    DOI 10.1016/j.aquatox.2016.07.004
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: TiO2 nanoparticles for the remediation of eutrophic shallow freshwater systems: Efficiency and impacts on aquatic biota under a microcosm experiment.

    Bessa da Silva, Márcia / Abrantes, Nelson / Nogueira, Verónica / Gonçalves, Fernando / Pereira, Ruth

    Aquatic toxicology (Amsterdam, Netherlands)

    2016  Volume 178, Page(s) 58–71

    Abstract: The application of nanomaterials (NMs) in the remediation of eutrophic waters, particularly in the control of internal loading of nutrients, has been started, but limited investigations evaluated the effectiveness of these new treatment approaches and of ...

    Abstract The application of nanomaterials (NMs) in the remediation of eutrophic waters, particularly in the control of internal loading of nutrients, has been started, but limited investigations evaluated the effectiveness of these new treatment approaches and of their potential impacts on species from shallow freshwater lakes. The present work investigated, under a microcosm experiment, the application of a TiO2 nanomaterial both for reducing nutrient (mainly phosphorus and nitrogen forms) desorption and release from sediments (preventive treatment-PT) and for eliminating algal blooms (remediation treatment-RT). Furthermore, we also intended to assess the potential impacts of nano-TiO2 application on key freshwater species. The results showed the effectiveness of nano-TiO2 in controlling the release of phosphates from surface sediment and the subsequent reduction of total phosphorus in the water column. A reduction in total nitrogen was also observed. Such changes in nutrient dynamics contributed to a progressive inhibition of development of algae after the application of the NM in PT microcosms. Concerning the ability of nano-TiO2 to interact with algal cells, this interaction has likely occurred, mainly in RT, enhancing the formation of aggregates and their rapid settlement, thus reducing the algal bloom. Both treatments caused deleterious effects on freshwater species. In PT, Daphnia magna and Lemna minor showed a significant inhibition of several endpoints. Conversely, no inhibitory effect on the growth of Chironomus riparius was recorded. In opposite, C. riparius was the most affected species in RT microcosms. Such difference was probably caused by the formation of larger TiO2-algae aggregates in RT, under a high algal density, that rapidly settled in the sediment, becoming less available for pelagic species. In summary, despite the effectiveness of both treatments in controlling internal nutrient loading and in the mitigating algal bloom episodes, their negative effects on biota have to be seriously taken into account.
    MeSH term(s) Animals ; Biota/drug effects ; Chironomidae/drug effects ; Daphnia/drug effects ; Environmental Restoration and Remediation ; Eutrophication/drug effects ; Geologic Sediments/chemistry ; Lakes/chemistry ; Nanoparticles/chemistry ; Nanoparticles/toxicity ; Nitrogen/pharmacology ; Phosphorus/pharmacology ; Portugal ; Titanium/chemistry ; Titanium/toxicity ; Water Pollutants, Chemical/chemistry ; Water Pollutants, Chemical/toxicity
    Chemical Substances Water Pollutants, Chemical ; titanium dioxide (15FIX9V2JP) ; Phosphorus (27YLU75U4W) ; Titanium (D1JT611TNE) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2016-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 782699-0
    ISSN 1879-1514 ; 0166-445X
    ISSN (online) 1879-1514
    ISSN 0166-445X
    DOI 10.1016/j.aquatox.2016.07.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Soil ecotoxicological screening (tier 1) for a diffuse-contaminated drainage area surrounding a lacustrine ecosystem in the Centre of Portugal

    Bessa da Silva, Márcia / Nelson Abrantes / Carla Patinha / Eduardo Ferreira da Silva / João Carlos Marques / Fernando Gonçalves / Ruth Pereira

    Journal of soils and sediments. 2018 Jan., v. 18, no. 1

    2018  

    Abstract: PURPOSE: This study presents a different approach for the application of the Dutch Risk Assessment Framework for contaminated sites, to areas undergoing diffuse pollution from agriculture activities. This approach aims to reduce the costs of tier 1, by ... ...

    Abstract PURPOSE: This study presents a different approach for the application of the Dutch Risk Assessment Framework for contaminated sites, to areas undergoing diffuse pollution from agriculture activities. This approach aims to reduce the costs of tier 1, by using the ecotoxicological line of evidence (EcotoxLoE) to select the soils for chemical analysis of potential contaminants and subsequently for an integrated evaluation of risks by combining both the chemical (ChemLoE) and the EcotoxLoE. MATERIALS AND METHODS: A battery of cost-effective and time-effective standard bioassays was applied, considering soil habitat function (whole soil approach—Microtox® test and avoidance assays with Folsomia candida) and soil retention function (elutriate approach—growth inhibition test with Raphidocelis subcapitata) for evaluating a vast array of samples collected in the study area. After a preliminary calculation of risks based on ecotoxicological data, samples displaying a moderate risk were screened for chemical analysis of the most used pesticides in the area, as well as for total metal concentrations after extraction following standard methods. For these samples, risks based on the ChemLoE and integrated risks were calculated. RESULTS AND DISCUSSION: The ChemLoE confirmed the evaluation made by the EcotoxLoE and reduced the level of risk (<0.5) for the samples formerly presenting a moderate risk. CONCLUSIONS: Given the sensitivity of the ecotoxicological assays to the mixture of contaminants potentially found in soils, the approach proved to be a good strategy for the application of the ERA framework, in particular of tier 1, on a routine basis, to areas under diffuse pollution. Since in these areas a more intense sampling is required, it can contribute to reducing the costs of the ChemLoE that can make the application of the ERA framework prohibitive.
    Keywords Folsomia candida ; Selenastrum capricornutum ; bioassays ; chemical analysis ; cost effectiveness ; drainage ; ecosystems ; ecotoxicology ; nonpoint source pollution ; pesticides ; risk ; risk assessment process ; screening ; soil ; soil habitats ; Portugal
    Language English
    Dates of publication 2018-01
    Size p. 189-204.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 2050898-0
    ISSN 1614-7480 ; 1439-0108
    ISSN (online) 1614-7480
    ISSN 1439-0108
    DOI 10.1007/s11368-017-1735-z
    Database NAL-Catalogue (AGRICOLA)

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