Article ; Online: An attenuated temperature-sensitive strain of cytomegalovirus (tsm5) establishes immunity without development of CD8(+) T cell memory inflation.
2013 Volume 85, Issue 11, Page(s) 1968–1974
Abstract: Cytomegalovirus (CMV) is a widely prevalent herpesvirus that is well tolerated by an immune competent host yet establishes a state of chronic infection. The virus is thought to undergo frequent subclinical episodes of reactivation which leads to an ... ...
| Abstract | Cytomegalovirus (CMV) is a widely prevalent herpesvirus that is well tolerated by an immune competent host yet establishes a state of chronic infection. The virus is thought to undergo frequent subclinical episodes of reactivation which leads to an unusually large accumulation of CMV-specific CD8(+) T lymphocytes in the peripheral blood, a phenomenon termed "memory inflation." The high magnitude of the CMV T cell response has been implicated in impaired immunity to heterologous pathogens such as EBV, influenza and West Nile virus. Here, using murine CMV (MCMV), we show that memory inflation of virus-specific CD8(+) T cells is avoided if mice are infected with a replication defective virus called temperature-sensitive mutant 5 (tsm5), which carries an attenuating mutation within the DNA primase gene. Mice infected with tsm5 do generate primary T cell responses towards viral proteins but these do not amass to skew the memory repertoire of CD8(+) T cells. Therefore, attenuation of the virus replication machinery may be valuable in future CMV vaccine designs because the virus remains immunogenic but does not contribute to CMV associated T cell immune senescence. |
|---|---|
| MeSH term(s) | Animals ; CD8-Positive T-Lymphocytes/immunology ; Cytomegalovirus Vaccines/administration & dosage ; Cytomegalovirus Vaccines/immunology ; Disease Models, Animal ; Female ; Herpesviridae Infections/immunology ; Herpesviridae Infections/virology ; Immunologic Memory ; Mice ; Mice, Inbred C57BL ; Muromegalovirus/immunology ; Muromegalovirus/physiology ; Mutation ; Vaccines, Attenuated/administration & dosage ; Vaccines, Attenuated/immunology ; Virulence ; Virus Replication |
| Chemical Substances | Cytomegalovirus Vaccines ; Vaccines, Attenuated |
| Language | English |
| Publishing date | 2013-11 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 752392-0 |
| ISSN | 1096-9071 ; 0146-6615 |
| ISSN (online) | 1096-9071 |
| ISSN | 0146-6615 |
| DOI | 10.1002/jmv.23688 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1320: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.