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  1. Article ; Online: Do "mastophages" hamper the histologic assessment of lymph node metastases in canine mast cell tumor?

    Sabattini, Silvia / Faroni, Eugenio / Zaccone, Riccardo / Marconato, Laura / Bettini, Giuliano

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2022  Volume 34, Issue 6, Page(s) 927–928

    MeSH term(s) Animals ; Dogs ; Lymphatic Metastasis/pathology ; Dog Diseases/pathology ; Carcinoma/pathology ; Carcinoma/veterinary ; Lymph Nodes/pathology
    Language English
    Publishing date 2022-09-30
    Publishing country United States
    Document type Letter
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387221127457
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The 2-tier grading system identifies canine cutaneous and/or subcutaneous mast cell tumors with aggressive biological behavior regardless of growth model.

    Sabattini, Silvia / Brocanelli, Alessia / Zaccone, Riccardo / Faroni, Eugenio / Renzi, Andrea / Ciammaichella, Luca / Bettini, Giuliano / Marconato, Laura

    Veterinary pathology

    2024  , Page(s) 3009858241240443

    Abstract: Histologic grading of canine cutaneous mast cell tumors (cMCTs) has prognostic and therapeutic implications, yet validation for subcutaneous MCTs (scMCTs) is lacking. For scMCTs with or without dermal invasion, determining their biological behavior ... ...

    Abstract Histologic grading of canine cutaneous mast cell tumors (cMCTs) has prognostic and therapeutic implications, yet validation for subcutaneous MCTs (scMCTs) is lacking. For scMCTs with or without dermal invasion, determining their biological behavior remains poorly standardized and sometimes sparks controversy. This prospective study aimed to assess the prognostic utility of the 2-tier histologic grading system in MCTs with different growth models (GMs) and explore the prognostic impact of the GM itself. We assessed 6 histologic GM categories: solely cMCT (C-SC
    Language English
    Publishing date 2024-04-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 188012-3
    ISSN 1544-2217 ; 0300-9858
    ISSN (online) 1544-2217
    ISSN 0300-9858
    DOI 10.1177/03009858241240443
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Grading Cutaneous Mast Cell Tumors in Cats

    Sabattini, Silvia / Bettini, Giuliano

    Veterinary Pathology. 2019 Jan., v. 56, no. 1 p.43-49

    2019  

    Abstract: Cutaneous mast cell tumors (cMCTs) account for approximately 20% of skin neoplasms in cats. As there is no grading system for these tumors, prognosis is difficult to estimate. Although the typical presentation is a benign tumor that can be cured by ... ...

    Abstract Cutaneous mast cell tumors (cMCTs) account for approximately 20% of skin neoplasms in cats. As there is no grading system for these tumors, prognosis is difficult to estimate. Although the typical presentation is a benign tumor that can be cured by surgical excision, a small but important proportion of feline cMCTs is biologically aggressive and can spread to local lymph nodes, precede the onset of disseminated cutaneous disease, or be associated with visceral involvement. A number of macroscopic and histologic features were retrospectively evaluated in cases of feline cMCTs treated with surgical excision with or without medical therapy. Cats were divided into 2 groups based on the clinical outcome. Group 1 included cats alive with no mast cell tumor–related disease at 1000 days from surgery; group 2 included cats developing histologically confirmed metastatic or cutaneous disseminated disease. The criteria allowing the best differentiation between the groups were used to develop a grading scheme. Groups 1 and 2 were composed by 48 (76%) and 15 (24%) cases, respectively. Tumors were classified as high grade if there were >5 mitotic figures in 10 fields (400×) and at least 2 of the following criteria: tumor diameter >1.5 cm, irregular nuclear shape, and nucleolar prominence/chromatin clusters. According to this scheme, the 15 (24%) high-grade cMCTs had significantly reduced survival time (median, 349 days; 95% CI, 0–739 days) as compared with the 48 low-grade tumors (median not reached; P < .001). Further studies are warranted to validate this grading system and test reproducibility on a larger case series.
    Keywords animal pathology ; cats ; chromatin ; excision ; histology ; lymph ; mast cells ; metastasis ; mitosis ; neoplasms ; prognosis ; skin ; feline ; neoplasm grading ; mast cell tumor ; mastocytoma
    Language English
    Dates of publication 2019-01
    Size p. 43-49.
    Publishing place SAGE Publications
    Document type Article ; Online
    ZDB-ID 188012-3
    ISSN 1544-2217 ; 0300-9858
    ISSN (online) 1544-2217
    ISSN 0300-9858
    DOI 10.1177/0300985818800028
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Grading Cutaneous Mast Cell Tumors in Cats.

    Sabattini, Silvia / Bettini, Giuliano

    Veterinary pathology

    2018  Volume 56, Issue 1, Page(s) 43–49

    Abstract: Cutaneous mast cell tumors (cMCTs) account for approximately 20% of skin neoplasms in cats. As there is no grading system for these tumors, prognosis is difficult to estimate. Although the typical presentation is a benign tumor that can be cured by ... ...

    Abstract Cutaneous mast cell tumors (cMCTs) account for approximately 20% of skin neoplasms in cats. As there is no grading system for these tumors, prognosis is difficult to estimate. Although the typical presentation is a benign tumor that can be cured by surgical excision, a small but important proportion of feline cMCTs is biologically aggressive and can spread to local lymph nodes, precede the onset of disseminated cutaneous disease, or be associated with visceral involvement. A number of macroscopic and histologic features were retrospectively evaluated in cases of feline cMCTs treated with surgical excision with or without medical therapy. Cats were divided into 2 groups based on the clinical outcome. Group 1 included cats alive with no mast cell tumor-related disease at 1000 days from surgery; group 2 included cats developing histologically confirmed metastatic or cutaneous disseminated disease. The criteria allowing the best differentiation between the groups were used to develop a grading scheme. Groups 1 and 2 were composed by 48 (76%) and 15 (24%) cases, respectively. Tumors were classified as high grade if there were >5 mitotic figures in 10 fields (400×) and at least 2 of the following criteria: tumor diameter >1.5 cm, irregular nuclear shape, and nucleolar prominence/chromatin clusters. According to this scheme, the 15 (24%) high-grade cMCTs had significantly reduced survival time (median, 349 days; 95% CI, 0-739 days) as compared with the 48 low-grade tumors (median not reached; P < .001). Further studies are warranted to validate this grading system and test reproducibility on a larger case series.
    MeSH term(s) Animals ; Cat Diseases/pathology ; Cat Diseases/surgery ; Cats ; Female ; Male ; Mastocytoma/pathology ; Mastocytoma/surgery ; Mastocytoma/veterinary ; Neoplasm Grading/veterinary ; Skin Neoplasms/pathology ; Skin Neoplasms/surgery ; Skin Neoplasms/veterinary ; Treatment Outcome
    Language English
    Publishing date 2018-09-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 188012-3
    ISSN 1544-2217 ; 0300-9858
    ISSN (online) 1544-2217
    ISSN 0300-9858
    DOI 10.1177/0300985818800028
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Mutational Analysis of c-KIT and PDGFRA in Canine Gastrointestinal Stromal Tumors (GISTs)

    Morini, Maria / Gentilini, Fabio / Turba, Maria Elena / Gobbo, Francesca / Mandrioli, Luciana / Bettini, Giuliano

    Veterinary sciences. 2022 July 21, v. 9, no. 7

    2022  

    Abstract: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the canine gastrointestinal tract and are diagnosed by the immunohistochemical expression of the receptor tyrosine kinase (RTK) KIT. Activating mutations of the proto- ... ...

    Abstract Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the canine gastrointestinal tract and are diagnosed by the immunohistochemical expression of the receptor tyrosine kinase (RTK) KIT. Activating mutations of the proto-oncogenes c-KIT and PDGFRA drive GIST oncogenesis and are used to predict the response to RTK-inhibitors in human oncology. Currently, the frequency and significance of these mutations in canine GIST have not been adequately explored. Therefore, we investigated the mutational status of c-KIT (exons 9, 11 and 13) and PDGFRA (exons 12 and 18) genes by PCR followed by fragment analysis for c-KIT deletions and PCR followed by screening with DHPLC and direct sequencing confirmation for single nucleotide variations in 17 formalin-fixed paraffin-embedded canine GISTs confirmed by KIT immunopositivity. c-KIT mutations were detected in 47% of cases, with a mutation detection rate significantly higher (p = 0.0004, Fisher’s exact test) and always involving exon 11. A PDGFRA gene mutation (exon 18) was identified in one case. Even if follow-up data were not available for all cases, four cases with documented abdominal metastases displayed c-KIT mutations. These data confirm that c-KIT exon 11 mutations occur frequently in canine GISTs, and identify the presence of a PDGFRA mutation similar to human GISTs. This study also suggests a potential association of c-KIT mutation with more aggressive biological behavior.
    Keywords carcinogenesis ; digestive tract ; dogs ; exons ; gastrointestinal system ; humans ; immunohistochemistry ; mutation ; mutational analysis ; proto-oncogenes ; receptor protein-tyrosine kinase
    Language English
    Dates of publication 2022-0721
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2768971-2
    ISSN 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9070376
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Mutational Analysis of

    Morini, Maria / Gentilini, Fabio / Turba, Maria Elena / Gobbo, Francesca / Mandrioli, Luciana / Bettini, Giuliano

    Veterinary sciences

    2022  Volume 9, Issue 7

    Abstract: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the canine gastrointestinal tract and are diagnosed by the immunohistochemical expression of the receptor tyrosine kinase (RTK) KIT. Activating mutations of the proto- ... ...

    Abstract Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the canine gastrointestinal tract and are diagnosed by the immunohistochemical expression of the receptor tyrosine kinase (RTK) KIT. Activating mutations of the proto-oncogenes
    Language English
    Publishing date 2022-07-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci9070376
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Longitudinal lymph node step-sectioning for the identification of metastatic disease in canine mast cell tumor.

    Sabattini, Silvia / Faroni, Eugenio / Renzi, Andrea / Ghisoni, Giulia / Rigillo, Antonella / Bettini, Giuliano / Pasquini, Arianna / Zanardi, Stefano / Guerra, Dina / Marconato, Laura

    Veterinary pathology

    2022  Volume 59, Issue 5, Page(s) 768–772

    Abstract: Lymph node (LN) metastasis in canine mast cell tumor (MCT) can affect prognosis and postsurgical treatment recommendations; however, routine histological single-section examination may underestimate the incidence of metastases. This prospective study ... ...

    Abstract Lymph node (LN) metastasis in canine mast cell tumor (MCT) can affect prognosis and postsurgical treatment recommendations; however, routine histological single-section examination may underestimate the incidence of metastases. This prospective study aimed at determining whether longitudinal step-sectioning of the entire LN allows for a more reliable detection of metastases. Dogs with MCT undergoing resection of the primary tumor and regional lymphadenectomy were enrolled. Formalin-fixed LNs were bisected longitudinally, both halves were embedded in paraffin and histological sections prepared at 200 μm steps. The nodal mast cells were classified according to the Weishaar classification. First-section evaluation (FSE; ie, examination of the first section obtained from the blocks) and whole LN step-section evaluation (SSE) were compared. Fifty-eight LNs were included. The median number of sections per LN was 6 (range, 3-28). FSE with toluidine blue (TB) revealed 27 (47%) nonmetastatic (HN0), 14 (24%) premetastatic (HN1), 9 (15%) early metastatic (HN2), and 8 (14%) overtly metastatic (HN3) LNs. SSE with TB resulted in upgrading the LN status in 2 cases (HN2 to HN3; HN0 to HN1). Evaluation of the first section plus an additional step-section resulted in 100% accuracy. Compared with SSE with TB, the accuracy of FSE with HE was 98% for HN3 LNs and 74% for HN2 LNs. FSE appears to reliably allow for the detection of LN metastasis in MCT, although examination of a further parallel section at a 200 μm step may increase the accuracy. A metachromatic stain is recommended for the identification of early metastases.
    MeSH term(s) Animals ; Dog Diseases/diagnosis ; Dog Diseases/pathology ; Dog Diseases/surgery ; Dogs ; Lymph Node Excision/methods ; Lymph Node Excision/veterinary ; Lymph Nodes/pathology ; Lymphatic Metastasis/diagnosis ; Lymphatic Metastasis/pathology ; Neoplasm Staging ; Prospective Studies
    Language English
    Publishing date 2022-04-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 188012-3
    ISSN 1544-2217 ; 0300-9858
    ISSN (online) 1544-2217
    ISSN 0300-9858
    DOI 10.1177/03009858221088780
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Ultrasonographic honeycomb pattern of the spleen in cats: correlation with pathological diagnosis in 33 cases.

    Quinci, Manuela / Sabattini, Silvia / Agnoli, Chiara / Bettini, Giuliano / Diana, Alessia

    Journal of feline medicine and surgery

    2019  Volume 22, Issue 8, Page(s) 800–804

    Abstract: Objectives: The aim of this study was to evaluate the relationship between the ultrasonographic (US) diffuse honeycomb pattern (HCP) of the spleen and a pathological diagnosis in cats, and to assess the influence of transducer type on HCP visualisation.! ...

    Abstract Objectives: The aim of this study was to evaluate the relationship between the ultrasonographic (US) diffuse honeycomb pattern (HCP) of the spleen and a pathological diagnosis in cats, and to assess the influence of transducer type on HCP visualisation.
    Methods: Abdominal ultrasounds of cats with an HCP were reviewed and splenic size, shape, margination, other parenchymal alterations and splenic hilar lymphadenopathy were recorded. When applicable, images acquired with high-frequency linear and curvilinear transducers were compared to determine if an HCP was more frequently demonstrated on high-resolution images. A retrospective review of the corresponding splenic cytohistopathological samples was also performed.
    Results: Thirty-three cats met the inclusion criteria. Five cases were diagnosed by histology and 28 by cytology, confirmed by PCR for antigen receptor rearrangements (PARR) in uncertain cases. There were 15 cases of lymphoid hyperplasia, eight cases of lymphoma (four B cell, three T cell and one large granular lymphocytes), six cats with splenitis, three with extramedullary haematopoiesis and one with histiocytic sarcoma. The prevalence of lymphoma in cats with an HCP of the spleen was 24%. Splenomegaly was the most frequent US feature associated with an HCP and was observed in all lymphoma cases. In the images obtained from both high-frequency linear and micro-convex transducers the visualisation of an HCP was enabled in all cases (24/24) and in 62.5% (15/24), respectively.
    Conclusions and relevance: Based on our findings, a US HCP of the spleen in cats can be associated with benign and malignant disorders and is infrequently associated with lymphoma in comparison with dogs. Cytological or histological examination, possibly supplemented by PARR, should always be performed for diagnostic support. Use of high-frequency linear transducers is recommended to properly recognise an HCP or subtle changes in splenic parenchyma.
    MeSH term(s) Animals ; Cat Diseases/diagnosis ; Cat Diseases/diagnostic imaging ; Cat Diseases/epidemiology ; Cats ; Female ; Italy/epidemiology ; Lymphoma/diagnosis ; Lymphoma/diagnostic imaging ; Lymphoma/epidemiology ; Lymphoma/veterinary ; Male ; Prevalence ; Retrospective Studies ; Splenic Diseases/diagnosis ; Splenic Diseases/diagnostic imaging ; Splenic Diseases/epidemiology ; Splenic Diseases/veterinary ; Ultrasonography/veterinary
    Language English
    Publishing date 2019-09-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2049047-1
    ISSN 1532-2750 ; 1098-612X
    ISSN (online) 1532-2750
    ISSN 1098-612X
    DOI 10.1177/1098612X19873197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Ultrasonographic honeycomb pattern of the spleen in cats: correlation with pathological diagnosis in 33 cases

    Quinci, Manuela / Sabattini, Silvia / Agnoli, Chiara / Bettini, Giuliano / Diana, Alessia

    Journal of feline medicine and surgery. 2020 Aug., v. 22, no. 8

    2020  

    Abstract: The aim of this study was to evaluate the relationship between the ultrasonographic (US) diffuse honeycomb pattern (HCP) of the spleen and a pathological diagnosis in cats, and to assess the influence of transducer type on HCP visualisation. Abdominal ... ...

    Abstract The aim of this study was to evaluate the relationship between the ultrasonographic (US) diffuse honeycomb pattern (HCP) of the spleen and a pathological diagnosis in cats, and to assess the influence of transducer type on HCP visualisation. Abdominal ultrasounds of cats with an HCP were reviewed and splenic size, shape, margination, other parenchymal alterations and splenic hilar lymphadenopathy were recorded. When applicable, images acquired with high-frequency linear and curvilinear transducers were compared to determine if an HCP was more frequently demonstrated on high-resolution images. A retrospective review of the corresponding splenic cytohistopathological samples was also performed. Thirty-three cats met the inclusion criteria. Five cases were diagnosed by histology and 28 by cytology, confirmed by PCR for antigen receptor rearrangements (PARR) in uncertain cases. There were 15 cases of lymphoid hyperplasia, eight cases of lymphoma (four B cell, three T cell and one large granular lymphocytes), six cats with splenitis, three with extramedullary haematopoiesis and one with histiocytic sarcoma. The prevalence of lymphoma in cats with an HCP of the spleen was 24%. Splenomegaly was the most frequent US feature associated with an HCP and was observed in all lymphoma cases. In the images obtained from both high-frequency linear and micro-convex transducers the visualisation of an HCP was enabled in all cases (24/24) and in 62.5% (15/24), respectively. Based on our findings, a US HCP of the spleen in cats can be associated with benign and malignant disorders and is infrequently associated with lymphoma in comparison with dogs. Cytological or histological examination, possibly supplemented by PARR, should always be performed for diagnostic support. Use of high-frequency linear transducers is recommended to properly recognise an HCP or subtle changes in splenic parenchyma.
    Keywords B-lymphocytes ; T-lymphocytes ; antigens ; cats ; combs (social insects) ; correlation ; dogs ; hematopoiesis ; histology ; hyperplasia ; lymphoma ; medicine ; parr ; prevalence ; sampling ; sarcoma ; shape ; spleen ; splenomegaly ; surgery ; transducers (equipment) ; ultrasonics ; ultrasonography
    Language English
    Dates of publication 2020-08
    Size p. 800-804.
    Publishing place SAGE Publications
    Document type Article
    Note NAL-light
    ZDB-ID 2049047-1
    ISSN 1532-2750 ; 1098-612X
    ISSN (online) 1532-2750
    ISSN 1098-612X
    DOI 10.1177/1098612X19873197
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Contrast‐enhanced ultrasonography in dogs with inflammatory bowel disease

    Linta, Nikolina / Pey, Pascaline / Baron Toaldo, Marco / Pietra, Marco / Felici, Manuel / Bettini, Giuliano / Cipone, Mario / Diana, Alessia

    Journal of veterinary internal medicine. 2021 Sept., v. 35, no. 5

    2021  

    Abstract: BACKGROUND: Contrast‐enhanced ultrasonography (CEUS) is used to evaluate vascularity of the gastrointestinal wall in neoplastic and inflammatory diseases. OBJECTIVE: To assess the feasibility of CEUS for the evaluation of duodenal perfusion in dogs with ... ...

    Abstract BACKGROUND: Contrast‐enhanced ultrasonography (CEUS) is used to evaluate vascularity of the gastrointestinal wall in neoplastic and inflammatory diseases. OBJECTIVE: To assess the feasibility of CEUS for the evaluation of duodenal perfusion in dogs with inflammatory bowel disease (IBD). ANIMALS: Forty‐two dogs with IBD and 20 clinically healthy dogs. METHODS: All CEUS studies of the duodenum were analyzed to obtain time‐intensity curves and perfusion parameters. The procedure was repeated in 12 IBD dogs 2 months after a standardized treatment. RESULTS: On CEUS, the duodenal wall showed a typical perfusion pattern characterized by a radial and simultaneous enhancement of the wall in all dogs. On qualitative assessment, no differences were observed in contrast medium distribution between healthy and affected dogs, or between dogs with IBD before and after treatment. Peak intensity (PI) and area under the curve (AUC) significantly differed between healthy (PI = 3.58 arbitrary units [au; 1.86‐4.93 au] and AUC = 47.63 au seconds [aus, 22.68‐62.15]) and affected dogs (PI = 5.10 au [0.63‐15.16 au] and AUC = 63.62 aus [5.31‐212.20 aus]; P = .03 and .03, respectively). No significant differences were found for the perfusion parameters before and after treatment. CONCLUSIONS AND CLINICAL IMPORTANCE: We showed that CEUS allows discrimination between IBD affected dogs and healthy dogs by evaluation of time‐intensity curves, but did not provide useful information for monitoring therapeutic response. The qualitative assessment identified no significant differences between healthy and affected dogs, or between dogs before and after treatment.
    Keywords duodenum ; inflammatory bowel disease ; therapeutics ; ultrasonography ; veterinary medicine
    Language English
    Dates of publication 2021-09
    Size p. 2167-2176.
    Publishing place John Wiley & Sons, Inc.
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 92798-3
    ISSN 1939-1676 ; 0891-6640
    ISSN (online) 1939-1676
    ISSN 0891-6640
    DOI 10.1111/jvim.16202
    Database NAL-Catalogue (AGRICOLA)

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