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  1. Article ; Online: Strategic transgene-free approaches of CRISPR-based genome editing in plants.

    Bhattacharjee, Sougata / Bhowmick, Rakesh / Kant, Lakshmi / Paul, Krishnayan

    Molecular genetics and genomics : MGG

    2023  Volume 298, Issue 3, Page(s) 507–520

    Abstract: Genome editing through the alteration of nucleotide sequence has already revolutionized the field of site-directed mutagenesis for a decade. However, research in terms of precision and efficacy in targeting the loci and reduction in off-target mutation ... ...

    Abstract Genome editing through the alteration of nucleotide sequence has already revolutionized the field of site-directed mutagenesis for a decade. However, research in terms of precision and efficacy in targeting the loci and reduction in off-target mutation has always been a priority when DNA is involved. Therefore, recent research interest lies in utilizing the same precision technology but results in non-transgenic. In this review article, different technological advancements have been explained, which may provide a holistic concept of and need for transgene-free genome editing. The advantage and lacunas of each technology have been critically discussed to deliver a transparent view to the readers. A systematic analysis and evaluation of published research articles implied that researchers across the globe are putting continuous efforts in this direction to eliminate the hindrance of transgenic regulation. Nevertheless, this approach has severe implications legitimate for mitigating the conflict of acceptance, reliability, and generosity of gene-editing technology and sustainably retorting to the expanding global population feeding challenges.
    MeSH term(s) Gene Editing/methods ; CRISPR-Cas Systems/genetics ; Reproducibility of Results ; Plants/genetics ; DNA ; Genome, Plant/genetics ; Plants, Genetically Modified/genetics
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2023-02-25
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2044817-X
    ISSN 1617-4623 ; 1617-4615
    ISSN (online) 1617-4623
    ISSN 1617-4615
    DOI 10.1007/s00438-023-01998-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1198: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: MethSemble-6mA: an ensemble-based 6mA prediction server and its application on promoter region of LBD gene family in Poaceae.

    Sinha, Dipro / Dasmandal, Tanwy / Paul, Krishnayan / Yeasin, Md / Bhattacharjee, Sougata / Murmu, Sneha / Mishra, Dwijesh Chandra / Pal, Soumen / Rai, Anil / Archak, Sunil

    Frontiers in plant science

    2023  Volume 14, Page(s) 1256186

    Abstract: The Lateral Organ Boundaries Domain (LBD) containing genes are a set of plant-specific transcription factors and are crucial for controlling both organ development and defense mechanisms as well as anthocyanin synthesis and nitrogen metabolism. It is ... ...

    Abstract The Lateral Organ Boundaries Domain (LBD) containing genes are a set of plant-specific transcription factors and are crucial for controlling both organ development and defense mechanisms as well as anthocyanin synthesis and nitrogen metabolism. It is imperative to understand how methylation regulates gene expression, through predicting methylation sites of their promoters particularly in major crop species. In this study, we developed a user-friendly prediction server for accurate prediction of 6mA sites by incorporating a robust feature set, viz., Binary Encoding of Mono-nucleotide DNA. Our model,MethSemble-6mA, outperformed other state-of-the-art tools in terms of accuracy (93.12%). Furthermore, we investigated the pattern of probable 6mA sites at the upstream promoter regions of the LBD-containing genes in
    Language English
    Publishing date 2023-10-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1256186
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Amelioration of cold-induced sweetening in potato by RNAi mediated silencing of

    Jaiswal, Sandeep / Paul, Krishnayan / Raman, K Venkat / Tyagi, Saurabh / Saakre, Manjesh / Tilgam, Jyotsana / Bhattacharjee, Sougata / Vijayan, Joshitha / Mondal, Kalyan Kumar / Sreevathsa, Rohini / Pattanayak, Debasis

    Frontiers in plant science

    2023  Volume 14, Page(s) 1133029

    Abstract: Cold-induced sweetening (CIS) is an unwanted physiological phenomenon in which reducing sugars (RS) get accumulated in potato ( ...

    Abstract Cold-induced sweetening (CIS) is an unwanted physiological phenomenon in which reducing sugars (RS) get accumulated in potato (
    Language English
    Publishing date 2023-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1133029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Identification, characterization, and comprehensive expression profiling of floral master regulators in pigeon pea (Cajanus cajan [L.] Millspaugh).

    Bhattacharjee, Sougata / Bhowmick, Rakesh / Paul, Krishnayan / Venkat Raman, K / Jaiswal, Sandeep / Tilgam, Jyotsana / Saakre, Manjesh / Kumari, Priyanka / Baaniya, Mahi / Vijayan, Joshitha / Sreevathsa, Rohini / Pattanayak, Debasis

    Functional & integrative genomics

    2023  Volume 23, Issue 4, Page(s) 311

    Abstract: Pigeon pea is an important protein-rich pulse crop. Identification of flowering master regulators in pigeon pea is highly imperative as indeterminacy and late flowering are impediments towards yield improvement. A genome-wide analysis was performed to ... ...

    Abstract Pigeon pea is an important protein-rich pulse crop. Identification of flowering master regulators in pigeon pea is highly imperative as indeterminacy and late flowering are impediments towards yield improvement. A genome-wide analysis was performed to explore flowering orthologous groups in pigeon pea. Among the 412 floral orthologs identified in pigeon pea, 148 genes belong to the meristem identity, photoperiod-responsive, and circadian clock-associated ortholog groups. Our comparative genomics study revealed purifying selection pressures (ka/ks) on floral orthologs, and duplication patterns and evolution through synteny with other model species. Phylogenetic analysis of floral genes substantiated a connection between pigeon pea plant architecture and flowering time as all the PEBP domain-containing genes belong to meristem identity floral networks of pigeon pea. Expression profiling of eleven major orthologs in contrasting determinate and indeterminate genotypes indicated that these orthologs might be involved in flowering regulation. Expression of floral inducer, FT, and floral repressor, TFL1, was non-comparable in indeterminate genotypes across all the developmental stages of pigeon pea. However, dynamic FT/TFL1 expression ratio detected in all tissues of both the genotypes suggested their role in floral transition. One TFL1 ortholog having high sequence conserveness across pigeon pea genotypes showed differential expression indicating genotype-dependent regulation of this ortholog. Presence of conserved 6mA-methylation patterns in light-responsive elements and in other cis-regulatory elements of FT and TFL1 across different plant genotypes indicated possible involvement of epigenetic regulation in flowering.
    MeSH term(s) Cajanus/genetics ; Epigenesis, Genetic ; Phylogeny ; Genotype ; Genomics
    Language English
    Publishing date 2023-09-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2014670-X
    ISSN 1438-7948 ; 1438-793X
    ISSN (online) 1438-7948
    ISSN 1438-793X
    DOI 10.1007/s10142-023-01236-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5230: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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