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  1. Article ; Online: Can LIGO Detect Nonannihilating Dark Matter?

    Bhattacharya, Sulagna / Dasgupta, Basudeb / Laha, Ranjan / Ray, Anupam

    Physical review letters

    2023  Volume 131, Issue 9, Page(s) 91401

    Abstract: Dark matter (DM) from the galactic halo can accumulate in neutron stars and transmute them into sub-2.5M_{⊙} black holes if the dark matter particles are heavy, stable, and have interactions with nucleons. We show that nondetection of gravitational waves ...

    Abstract Dark matter (DM) from the galactic halo can accumulate in neutron stars and transmute them into sub-2.5M_{⊙} black holes if the dark matter particles are heavy, stable, and have interactions with nucleons. We show that nondetection of gravitational waves from mergers of such low-mass black holes can constrain the interactions of nonannihilating dark matter particles with nucleons. We find benchmark constraints with LIGO O3 data, viz., σ_{χn}≥O(10^{-47})  cm^{2} for bosonic DM with m_{χ}∼PeV (or m_{χ}∼GeV, if they can Bose-condense) and ≥O(10^{-46})  cm^{2} for fermionic DM with m_{χ}∼10^{3}  PeV. These bounds depend on the priors on DM parameters and on the currently uncertain binary neutron star merger rate density. However, with increased exposure by the end of this decade, LIGO will probe cross sections that are many orders of magnitude below the neutrino floor and completely test the dark matter solution to missing pulsars in the Galactic center, demonstrating a windfall science case for gravitational wave detectors as probes of particle dark matter.
    Language English
    Publishing date 2023-09-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.131.091401
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Role of Platelet Cytokines in Dengue Virus Infection.

    Singh, Anamika / Bisht, Piyush / Bhattacharya, Sulagna / Guchhait, Prasenjit

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 561366

    Abstract: Platelets are anucleated blood cells derived from bone marrow megakaryocytes and play a crucial role in hemostasis and thrombosis. Platelets contain specialized storage organelles, called alpha-granules, contents of which are rich in cytokines such as C- ... ...

    Abstract Platelets are anucleated blood cells derived from bone marrow megakaryocytes and play a crucial role in hemostasis and thrombosis. Platelets contain specialized storage organelles, called alpha-granules, contents of which are rich in cytokines such as C-X-C Motif Chemokine Ligand (CXCL) 1/4/7, (C-C motif) ligand (CCL) 5/3, CXCL8 (also called as interleukin 8, IL-8), and transforming growth factor β (TGF-β). Activation of platelets lead to degranulation and release of contents into the plasma. Platelet activation is a common event in many viral infections including human immunodeficiency virus (HIV), H1N1 influenza, Hepatitis C virus (HCV), Ebola virus (EBV), and Dengue virus (DENV). The cytokines CXCL8, CCL5 (also known as Regulated on Activation, Normal T Expressed and Secreted, RANTES), tumor necrosis factor α (TNF-α), CXCL1/5 and CCL3 released, promote development of a pro-inflammatory state along with the recruitment of other immune cells to the site of infection. Platelets also interact with Monocytes and Neutrophils and facilitate their activation to release different cytokines which further enhances inflammation. Upon activation, platelets also secrete factors such as CXCL4 (also known as platelet factor, PF4), CCL5 and fibrinopeptides which are critical regulators of replication and propagation of several viruses in the host. Studies suggest that CXCL4 can both inhibit as well as enhance HIV1 infection. Data from our lab show that CXCL4 inhibits interferon (IFN) pathway and promotes DENV replication in monocytes
    MeSH term(s) Blood Platelets ; Cytokines ; Dengue ; Dengue Virus ; Humans ; Influenza A Virus, H1N1 Subtype
    Chemical Substances Cytokines
    Keywords covid19
    Language English
    Publishing date 2020-09-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.561366
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Interplay between hypoxia and inflammation contributes to the progression and severity of respiratory viral diseases.

    Bhattacharya, Sulagna / Agarwal, Sakshi / Shrimali, Nishith M / Guchhait, Prasenjit

    Molecular aspects of medicine

    2021  Volume 81, Page(s) 101000

    Abstract: History of pandemics is dominated by viral infections and specifically respiratory viral diseases like influenza and COVID-19. Lower respiratory tract infection is the fourth leading cause of death worldwide. Crosstalk between resultant inflammation and ... ...

    Abstract History of pandemics is dominated by viral infections and specifically respiratory viral diseases like influenza and COVID-19. Lower respiratory tract infection is the fourth leading cause of death worldwide. Crosstalk between resultant inflammation and hypoxic microenvironment may impair ventilatory response of lungs. This reduces arterial partial pressure of oxygen, termed as hypoxemia, which is observed in a section of patients with respiratory virus infections including SARS-CoV-2 (COVID-19). In this review, we describe the interplay between inflammation and hypoxic microenvironment in respiratory viral infection and its contribution to disease pathogenesis.
    MeSH term(s) COVID-19 ; Humans ; Hypoxia ; Inflammation ; Pandemics ; SARS-CoV-2
    Language English
    Publishing date 2021-07-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 197640-0
    ISSN 1872-9452 ; 0098-2997
    ISSN (online) 1872-9452
    ISSN 0098-2997
    DOI 10.1016/j.mam.2021.101000
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Gain-of-function Tibetan PHD2

    Bhattacharya, Sulagna / Shrimali, Nishith M / Mohammad, Ghulam / Koul, Parvaiz A / Prchal, Josef T / Guchhait, Prasenjit

    EBioMedicine

    2021  Volume 68, Page(s) 103418

    Abstract: Background: We have previously described an evolutionarily selected Tibetan prolyl hydroxylase-2 (PHD2: Methods: We genotyped the Tibetans using DNA isolated from whole blood. Thereafter immunophenotying was performed on PBMCs from homozygous PHD2: ...

    Abstract Background: We have previously described an evolutionarily selected Tibetan prolyl hydroxylase-2 (PHD2
    Methods: We genotyped the Tibetans using DNA isolated from whole blood. Thereafter immunophenotying was performed on PBMCs from homozygous PHD2
    Findings: We report that homozygous PHD2
    Interpretation: Our report suggests that gain-of-function PHD2
    Funding: This study is supported by the Department of Biotechnology, Government of India.
    MeSH term(s) Adult ; Altitude Sickness/chemically induced ; Altitude Sickness/genetics ; Altitude Sickness/prevention & control ; Animals ; Case-Control Studies ; Disease Models, Animal ; Female ; Gain of Function Mutation ; Humans ; Hypertension, Pulmonary/chemically induced ; Hypertension, Pulmonary/genetics ; Hypertension, Pulmonary/prevention & control ; Hypoxia-Inducible Factor-Proline Dioxygenases/genetics ; Immunophenotyping ; Ketoglutaric Acids/adverse effects ; Male ; Mice ; Travel ; U937 Cells ; Young Adult
    Chemical Substances Ketoglutaric Acids ; EGLN1 protein, human (EC 1.14.11.2) ; Hypoxia-Inducible Factor-Proline Dioxygenases (EC 1.14.11.29)
    Language English
    Publishing date 2021-06-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2021.103418
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Nano-enhanced Optical Gene Delivery to Retinal Degenerated Mice.

    Batabyal, Subrata / Gajjeraman, Sivakumar / Bhattacharya, Sulagna / Wright, Weldon / Mohanty, Samarendra

    Current gene therapy

    2019  Volume 19, Issue 5, Page(s) 318–329

    Abstract: Background: The efficient and targeted delivery of genes and other impermeable therapeutic molecules into retinal cells is of immense importance for the therapy of various visual disorders. Traditional methods for gene delivery require viral ... ...

    Abstract Background: The efficient and targeted delivery of genes and other impermeable therapeutic molecules into retinal cells is of immense importance for the therapy of various visual disorders. Traditional methods for gene delivery require viral transfection, or chemical methods that suffer from one or many drawbacks, such as low efficiency, lack of spatially targeted delivery, and can generally have deleterious effects, such as unexpected inflammatory responses and immunological reactions.
    Methods: We aim to develop a continuous wave near-infrared laser-based Nano-enhanced Optical Delivery (NOD) method for spatially controlled delivery of ambient-light-activatable Muti-Characteristic opsin-encoding genes into retina in-vivo and ex-vivo. In this method, the optical field enhancement by gold nanorods is utilized to transiently permeabilize cell membrane, enabling delivery of exogenous impermeable molecules to nanorod-binding cells in laser-irradiated regions.
    Results and discussion: With viral or other non-viral (e.g. electroporation, lipofection) methods, gene is delivered everywhere, causing uncontrolled expression over the whole retina. This will cause complications in the functioning of non-degenerated areas of the retina. In the NOD method, the contrast in temperature rise in laser-irradiated nanorod-attached cells at nano-hotspots is significant enough to allow site-specific delivery of large genes. The in-vitro and in-vivo results using NOD, clearly demonstrate in-vivo gene delivery and functional cellular expression in targeted retinal regions without compromising the structural integrity of the eye or causing immune response.
    Conclusion: The successful delivery and expression of MCO in the targeted retina after in-vivo NOD in the mice models of retinal degeneration opens a new vista for re-photosensitizing retina with geographic atrophies, such as in dry age-related macular degeneration.
    MeSH term(s) Animals ; Disease Models, Animal ; Gene Transfer Techniques ; Genetic Therapy/trends ; HEK293 Cells ; Humans ; Macular Degeneration/genetics ; Macular Degeneration/therapy ; Mice ; Nanoparticles/therapeutic use ; Retina/pathology ; Retinal Degeneration/genetics ; Retinal Degeneration/therapy ; Visual Fields
    Language English
    Publishing date 2019-10-14
    Publishing country United Arab Emirates
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2146187-9
    ISSN 1875-5631 ; 1566-5232
    ISSN (online) 1875-5631
    ISSN 1566-5232
    DOI 10.2174/1566523219666191017114044
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Sensitization of ON-bipolar cells with ambient light activatable multi-characteristic opsin rescues vision in mice.

    Batabyal, Subrata / Gajjeraman, Sivakumar / Pradhan, Sanjay / Bhattacharya, Sulagna / Wright, Weldon / Mohanty, Samarendra

    Gene therapy

    2020  Volume 28, Issue 3-4, Page(s) 162–176

    Abstract: Gene therapy-based treatment such as optogenetics offers a potentially powerful way to bypass damaged photoreceptors in retinal degenerative diseases and use the remaining retinal cells for functionalization to achieve photosensitivity. However, current ... ...

    Abstract Gene therapy-based treatment such as optogenetics offers a potentially powerful way to bypass damaged photoreceptors in retinal degenerative diseases and use the remaining retinal cells for functionalization to achieve photosensitivity. However, current approaches of optogenetic treatment rely on opsins that require high intensity light for activation thus adding to the challenge for use as part of a wearable device. Here, we report AAV2 assisted delivery of highly photosensitive multi-characteristic opsin (MCO1) into ON-bipolar cells of mice with retinal degeneration to allow activation by ambient light. Rigorous characterization of delivery efficacy by different doses of AAV2 carrying MCO1 (vMCO1) into targeted cells showed durable expression over 6 months after delivery as measured by reporter expression. The enduring MCO1 expression was correlated with the significantly improved behavioral outcome, that was longitudinally measured by visual water-maze and optomotor assays. The pro/anti-inflammatory cytokine levels in plasma and vitreous humor of the vMCO1-injected group did not change significantly from baseline or control group. Furthermore, biodistribution studies at various time points after injection in animal groups injected with different doses of vMCO1 showed non-detectable vector copies in non-targeted tissues. Immunohistochemistry of vMCO1 transfected retinal tissues showed bipolar specific expression of MCO1 and the absence of immune/inflammatory response. Furthermore, ocular imaging using SD-OCT showed no change in the structural architecture of vMCO1-injected eyes. Induction of ambient light responsiveness to remaining healthy bipolar cells in subjects with retinal degeneration will allow the retinal circuitry to gain visual acuity without requiring an active stimulation device.
    MeSH term(s) Animals ; Mice ; Opsins/genetics ; Opsins/metabolism ; Retinal Degeneration/genetics ; Retinal Degeneration/therapy ; Rod Opsins/metabolism ; Tissue Distribution ; Vision, Ocular
    Chemical Substances Opsins ; Rod Opsins
    Language English
    Publishing date 2020-10-22
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1191036-7
    ISSN 1476-5462 ; 0969-7128
    ISSN (online) 1476-5462
    ISSN 0969-7128
    DOI 10.1038/s41434-020-00200-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: α-Ketoglutarate Inhibits Thrombosis and Inflammation by Prolyl Hydroxylase-2 Mediated Inactivation of Phospho-Akt.

    Shrimali, Nishith M / Agarwal, Sakshi / Kaur, Simrandeep / Bhattacharya, Sulagna / Bhattacharyya, Sankar / Prchal, Josef T / Guchhait, Prasenjit

    EBioMedicine

    2021  Volume 73, Page(s) 103672

    Abstract: Background: Phospho-Akt1 (pAkt1) undergoes prolyl hydroxylation at Pro125 and Pro313 by the prolyl hydroxylase-2 (PHD2) in a reaction decarboxylating α-ketoglutarate (αKG). We investigated whether the αKG supplementation could inhibit Akt-mediated ... ...

    Abstract Background: Phospho-Akt1 (pAkt1) undergoes prolyl hydroxylation at Pro125 and Pro313 by the prolyl hydroxylase-2 (PHD2) in a reaction decarboxylating α-ketoglutarate (αKG). We investigated whether the αKG supplementation could inhibit Akt-mediated activation of platelets and monocytes, in vitro as well as in vivo, by augmenting PHD2 activity.
    Methods: We treated platelets or monocytes isolated from healthy individuals with αKG in presence of agonists in vitro and assessed the signalling molecules including pAkt1. We supplemented mice with dietary αKG and estimated the functional responses of platelets and monocytes ex vivo. Further, we investigated the impact of dietary αKG on inflammation and thrombosis in lungs of mice either treated with thrombosis-inducing agent carrageenan or infected with SARS-CoV-2.
    Findings: Octyl αKG supplementation to platelets promoted PHD2 activity through elevated intracellular αKG to succinate ratio, and reduced aggregation in vitro by suppressing pAkt1(Thr308). Augmented PHD2 activity was confirmed by increased hydroxylated-proline and enhanced binding of PHD2 to pAkt in αKG-treated platelets. Contrastingly, inhibitors of PHD2 significantly increased pAkt1 in platelets. Octyl-αKG followed similar mechanism in monocytes to inhibit cytokine secretion in vitro. Our data also describe a suppressed pAkt1 and reduced activation of platelets and leukocytes ex vivo from mice supplemented with dietary αKG, unaccompanied by alteration in their number. Dietary αKG significantly reduced clot formation and leukocyte accumulation in various organs including lungs of mice treated with thrombosis-inducing agent carrageenan. Importantly, in SARS-CoV-2 infected hamsters, we observed a significant rescue effect of dietary αKG on inflamed lungs with significantly reduced leukocyte accumulation, clot formation and viral load alongside down-modulation of pAkt in the lung of the infected animals.
    Interpretation: Our study suggests that dietary αKG supplementation prevents Akt-driven maladies such as thrombosis and inflammation and rescues pathology of COVID19-infected lungs.
    Funding: Study was funded by the Department of Biotechnology (DBT), Govt. of India (grants: BT/PR22881 and BT/PR22985); and the Science and Engineering Research Board, Govt. of India (CRG/000092).
    MeSH term(s) Animals ; Blood Platelets/cytology ; Blood Platelets/drug effects ; Blood Platelets/metabolism ; COVID-19/pathology ; COVID-19/prevention & control ; COVID-19/veterinary ; COVID-19/virology ; Cricetinae ; Dietary Supplements ; Down-Regulation/drug effects ; Humans ; Ketoglutaric Acids/pharmacology ; Ketoglutaric Acids/therapeutic use ; Lung/metabolism ; Lung/pathology ; Mesocricetus ; Mice ; Mice, Inbred BALB C ; Monocytes/cytology ; Monocytes/drug effects ; Monocytes/metabolism ; Phosphorylation ; Platelet Aggregation/drug effects ; Prolyl Hydroxylases/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/physiology ; Thrombosis/chemically induced ; Thrombosis/pathology ; Thrombosis/prevention & control ; Thrombosis/veterinary
    Chemical Substances Ketoglutaric Acids ; Protein Isoforms ; Prolyl Hydroxylases (EC 1.14.11.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
    Language English
    Publishing date 2021-11-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2021.103672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Goods induced service growth or the other way round?

    Datta, Madhusudan / Bhattacharya, Sulagna

    Artha vijñāna : journal of the Gokhale Institute of Politics and Economics Vol. 51, No. 3 , p. 283-296

    2009  Volume 51, Issue 3, Page(s) 283–296

    Author's details Madhusudan Datta and Sulagna Bhattacharya
    Keywords Dienstleistungssektor ; Finanzdienstleistung ; Branchenentwicklung ; Indien
    Language English
    Publishing place Pune
    Document type Article
    ZDB-ID 303536-0
    ISSN 0004-3559
    Database ECONomics Information System

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  9. Article: Restoring vision in mice with retinal degeneration using multicharacteristic opsin.

    Wright, Weldon / Gajjeraman, Sivakumar / Batabyal, Subrata / Pradhan, Sanjay / Bhattacharya, Sulagna / Mahapatra, Vasu / Tripathy, Ashutosh / Mohanty, Samarendra

    Neurophotonics

    2017  Volume 4, Issue 4, Page(s) 41412

    Abstract: Retinal degenerative diseases, such as retinitis pigmentosa (RP) and dry age-related macular degeneration, have led to loss of vision in millions of individuals. Currently, no surgical or medical treatment is available, although optogenetic therapies are ...

    Abstract Retinal degenerative diseases, such as retinitis pigmentosa (RP) and dry age-related macular degeneration, have led to loss of vision in millions of individuals. Currently, no surgical or medical treatment is available, although optogenetic therapies are in clinical development. We demonstrate vision restoration using multicharacteristics opsin (MCO1) in animal models with degenerated retina. MCO1 is reliably delivered to specific retinal cells via intravitreal injection of adeno-associated virus (vMCO1), leading to significant improvement in visually guided behavior conducted using a radial arm water maze. The time to reach the platform and the number of error arms decreased significantly after delivery of MCO1. Notably, the improvement in visually guided behavior was observed even at light intensity levels orders of magnitude lower than that required for channelrhodopsin-2 opsin. Viability of vMCO1-treated retina is not compromised by chronic light exposure. Safe virus-mediated MCO1 delivery has potential for effective gene therapy of diverse retinal degenerations in patients.
    Language English
    Publishing date 2017-08-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2781943-7
    ISSN 2329-4248 ; 2329-423X
    ISSN (online) 2329-4248
    ISSN 2329-423X
    DOI 10.1117/1.NPh.4.4.041412
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Restoring vision in mice with retinal degeneration using multicharacteristic opsin.

    Wright, Weldon / Gajjeraman, Sivakumar / Batabyal, Subrata / Pradhan, Sanjay / Bhattacharya, Sulagna / Mahapatra, Vasu / Tripathy, Ashutosh / Mohanty, Samarendra

    Neurophotonics

    2017  Volume 4, Issue 4, Page(s) 41505

    Abstract: Retinal degenerative diseases, such as retinitis pigmentosa (RP) and dry age-related macular degeneration, have led to loss of vision in millions of individuals. Currently, no surgical or medical treatment is available, although optogenetic therapies are ...

    Abstract Retinal degenerative diseases, such as retinitis pigmentosa (RP) and dry age-related macular degeneration, have led to loss of vision in millions of individuals. Currently, no surgical or medical treatment is available, although optogenetic therapies are in clinical development. We demonstrate vision restoration using multicharacteristics opsin (MCO1) in animal models with degenerated retina. MCO1 is reliably delivered to specific retinal cells via intravitreal injection of adeno-associated virus (vMCO1), leading to significant improvement in visually guided behavior conducted using a radial arm water maze. The time to reach the platform and the number of error arms decreased significantly after delivery of MCO1. Notably, the improvement in visually guided behavior was observed even at light intensity levels orders of magnitude lower than that required for channelrhodopsin-2 opsin. Viability of vMCO1-treated retina is not compromised by chronic light exposure. Safe virus-mediated MCO1 delivery has potential for effective gene therapy of diverse retinal degenerations in patients.
    Language English
    Publishing date 2017-08-18
    Publishing country United States
    Document type Journal Article ; Corrected and Republished Article
    ZDB-ID 2781943-7
    ISSN 2329-4248 ; 2329-423X
    ISSN (online) 2329-4248
    ISSN 2329-423X
    DOI 10.1117/1.NPh.4.4.041505
    Database MEDical Literature Analysis and Retrieval System OnLINE

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