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Article ; Online: The global regulator SpoVG regulates Listeria monocytogenes biofilm formation.

Shi, Changzheng / Zheng, Liping / Lu, Zhaoxin / Zhang, Xinyi / Bie, Xiaomei

2023 Volume 180, Page(s) 106144

Abstract: Biofilms provide a suitable environment for L. monocytogenes and are the cause of enormous risks in the food industry. SpoVG is a global regulatory factor that plays a vital role in physiological activity of L. monocytogenes. We constructed spoVG mutant ... ...

Abstract	Biofilms provide a suitable environment for L. monocytogenes and are the cause of enormous risks in the food industry. SpoVG is a global regulatory factor that plays a vital role in physiological activity of L. monocytogenes. We constructed spoVG mutant strains to investigate the effects of these mutants on L. monocytogenes biofilms. The results show that L. monocytogenes biofilm formation was decreased by 40%. Furthermore, we measured biofilm related phenotypes to study the regulation of SpoVG. The motility capacity of L. monocytogenes was found to decrease after the deletion of spoVG. The cell surface properties changed in the spoVG mutant strains, with an increase in both the cell surface hydrophobicity and the auto-aggregation capacity after spoVG deletion. SpoVG mutant strains were found to be more sensitive to antibiotics, and had a reduced tolerance to inappropriate pH, salt stress and low temperature. The RT-qPCR results showed that SpoVG effectively regulated the expression of genes related to quorum sensing, flagella, virulence and stress factors. These findings suggest that spoVG has potential as a target to decrease biofilm formation and control L. monocytogenes contamination in the food industry.
MeSH term(s)	Listeria monocytogenes ; Temperature ; Bacterial Proteins/metabolism ; Biofilms ; Virulence/genetics
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2023-05-04
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2023.106144
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Zs.A 2136: Show issues

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Article ; Online: Effects of the deletion and substitution of thioesterase on bacillomycin D synthesis.

Zhang, Ping / Lv, Ziyan / Lu, Zhaoxin / Ma, Wenjie / Bie, Xiaomei

Biotechnology letters

2023 Volume 45, Issue 8, Page(s) 981–991

Abstract: Objectives: The importance of thioesterase domains on bacillomycin D synthesis and the ability of different thioesterase domains to selectively recognize and catalyze peptide chain hydrolysis and cyclization were studied by deleting and substituting ... ...

Abstract	Objectives: The importance of thioesterase domains on bacillomycin D synthesis and the ability of different thioesterase domains to selectively recognize and catalyze peptide chain hydrolysis and cyclization were studied by deleting and substituting thioesterase domains. Results: No bacillomycin D analogs were found in the thioesterase-deleted strain fmbJ-ΔTE, indicating that the TE domain was essential for bacillomycin D synthesis. Then the thioesterase in bacillomycin D synthetases was replaced by the thioesterase in bacillomycin F, iturin A, mycosubtilin, plipastatin and surfactin synthetases. Except for fmbJ-S-TE, all others were able to synthesize bacillomycin D homologs because a suitable recombination site was selected, which maintained the integrity of NRPSs. In particular, the yield of bacillomycin D in fmbJ-IA-TE, fmbJ-M-TE and fmbJ-P-TE was significantly increased. Conclusion: This study expands our understanding of the TE domain in bacillomycin D synthetases and shows that thioesterase has excellent potential in the chemical-enzymatic synthesis of natural products or their analogs.
Language	English
Publishing date	2023-06-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	423853-9
ISSN	1573-6776 ; 0141-5492
ISSN (online)	1573-6776
ISSN	0141-5492
DOI	10.1007/s10529-023-03373-z
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Zs.A 4564: Show issues

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Article ; Online: A duplex real-time NASBA assay targeting a serotype-specific gene for rapid detection of viable

Zhai, Ligong / Liu, Hongxia / Li, Junjie / Lu, Zhaoxin / Bie, Xiaomei

Canadian journal of microbiology

2022 Volume 68, Issue 4, Page(s) 259–268

Abstract: Salmonella ... ...

Abstract	Salmonella enterica
MeSH term(s)	Animals ; Food Microbiology ; Salmonella/genetics ; Salmonella paratyphi A/genetics ; Salmonella paratyphi C/genetics ; Self-Sustained Sequence Replication ; Serogroup
Language	English
Publishing date	2022-01-13
Publishing country	Canada
Document type	Journal Article
ZDB-ID	280534-0
ISSN	1480-3275 ; 0008-4166
ISSN (online)	1480-3275
ISSN	0008-4166
DOI	10.1139/cjm-2021-0054
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Article ; Online: Biochemical and molecular regulatory mechanism of the pgpH gene on biofilm formation in Listeria monocytogenes.

Zhang, Xinyi / Zheng, Liping / Lu, Zhaoxin / Zhou, Libang / Meng, Fanqiang / Shi, Changzheng / Bie, Xiaomei

Journal of applied microbiology

2023 Volume 134, Issue 2

Abstract: Aims: PgpH gene has an important regulatory role on bacterial physiological activity, but studies on its regulation mechanism on biofilm formation of Listeria monocytogenes are lacking. Our aim was to investigate the effect of pgpH gene deletion on ... ...

Abstract	Aims: PgpH gene has an important regulatory role on bacterial physiological activity, but studies on its regulation mechanism on biofilm formation of Listeria monocytogenes are lacking. Our aim was to investigate the effect of pgpH gene deletion on biofilm formation in L. monocytogenes. Methods and results: The ΔpgpH deletion strain of L. monocytogenes LMB 33 426 was constructed by homologous recombination. Deletion of the pgpH gene resulted in a significant reduction in biofilm formation. The swimming ability of the ΔpgpH strain on semisolid plates was unchanged compared to the wild-type strain (WT), and the auto-aggregation capacity of L. monocytogenes was decreased. RNA-seq showed that ΔpgpH resulted in the differential expression of 2357 genes compared to WT. pgpH inactivation resulted in the significant downregulation of the cell wall formation-related genes dltC, dltD, walK, and walR and the flagellar assembly related genes fliG and motB. Conclusions: This study shows that the deletion of pgpH gene regulates biofilm formation and auto-aggregation ability of L. monocytogenes by affecting the expression of flagellar assembly and cell wall related genes. pgpH has a global regulatory effect on biofilm formation in L. monocytogenes.
MeSH term(s)	Biofilms ; Listeria monocytogenes/physiology ; Gene Deletion ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2023-01-18
Publishing country	England
Document type	Journal Article
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1093/jambio/lxac086
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Zs.A 259: Show issues

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Article ; Online: Prevention of high-fat-diet-induced obesity in mice by soluble dietary fiber from fermented and unfermented millet bran.

Yang, Duo / Shen, Juan / Tang, Chao / Lu, Zhaoxin / Lu, Fengxia / Bie, Xiaomei / Meng, Fanqiang / Zhao, Haizhen

Food research international (Ottawa, Ont.)

2024 Volume 179, Page(s) 113974

Abstract: Obesity-related diabetes, cardiovascular disease, and hypertension pose many risks to human health. Thus, mice on a high-fat diet were gavaged with millet bran (unfermented/fermented) soluble dietary fiber (RSDF/FSDF, 500 mg· ... ...

Abstract	Obesity-related diabetes, cardiovascular disease, and hypertension pose many risks to human health. Thus, mice on a high-fat diet were gavaged with millet bran (unfermented/fermented) soluble dietary fiber (RSDF/FSDF, 500 mg·kg
MeSH term(s)	Mice ; Humans ; Animals ; Diet, High-Fat/adverse effects ; Millets ; Obesity ; Cholesterol ; Dietary Fiber
Chemical Substances	Cholesterol (97C5T2UQ7J) ; Dietary Fiber
Language	English
Publishing date	2024-01-10
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2024.113974
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Article ; Online: Establishment of real-time fluorescence and visual LAMP for rapid detection of Escherichia coli O157:H7 and kits construction.

Wang, Zuwei / Cui, Xinping / Hu, Antuo / Lu, Zhaoxin / Meng, Fanqiang / Zhou, Libang / Bie, Xiaomei

Letters in applied microbiology

2023 Volume 76, Issue 11

Abstract: Escherichia coli O157:H7 is a common pathogenic bacterium in food and water that can pose a threat to human health. The aim of this study was to develop loop-mediated isothermal amplification (LAMP) method for the detection of E. coli O157:H7 in food ... ...

Abstract	Escherichia coli O157:H7 is a common pathogenic bacterium in food and water that can pose a threat to human health. The aim of this study was to develop loop-mediated isothermal amplification (LAMP) method for the detection of E. coli O157:H7 in food based on the specific gene Ecs_2840 and to construct rapid detection kits based on the established methods. Specifically, we established two methods of real-time fluorescent LAMP (RT-LAMP) and visual LAMP with calcein as an indicator. In pure bacterial culture, the cell sensitivity and genomic sensitivity of the RT-LAMP kit were 8.8 × 100 CFU ml-1 and 4.61 fg µl-1, respectively. The sensitivity of the visual LAMP kit was 2.35 × 100 CFU ml-1 and 4.61 fg µl-1. Both kits had excellent specificity and anti-interference performance. In addition, milk inoculated with 2.26 × 100 CFU ml-1E. coli O157:H7 could be detected within the reaction time after enrichment for 3 h. The results showed that the LAMP kits were rapid, sensitive, and specific for the detection of E. coli O157:H7 in food and had good application prospects in food safety surveillance.
MeSH term(s)	Humans ; Escherichia coli O157/genetics ; Sensitivity and Specificity ; Food Microbiology
Language	English
Publishing date	2023-10-19
Publishing country	England
Document type	Journal Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1093/lambio/ovad122
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Zs.A 2080: Show issues

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Article ; Online: Drug-loaded lipid nanoparticles improve the removal rates of the Staphylococcus aureus biofilm.

Qiao, Jiaju / Hu, Antuo / Zhou, Haibo / Lu, Zhaoxin / Meng, Fanqiang / Shi, Changzheng / Bie, Xiaomei

Biotechnology journal

2023 Volume 19, Issue 2, Page(s) e2300159

Abstract: Biofilms of the foodborne pathogen Staphylococcus aureus show improved resistance to antibiotics and are difficult to eliminate. To enhance antibacteria and biofilm dispersion via extracellular matrix diffusion, a new lipid nanoparticle was prepared, ... ...

Abstract	Biofilms of the foodborne pathogen Staphylococcus aureus show improved resistance to antibiotics and are difficult to eliminate. To enhance antibacteria and biofilm dispersion via extracellular matrix diffusion, a new lipid nanoparticle was prepared, which employed a mixture of phospholipids and a 0.8% surfactin shell. In the lipid nanoparticle, 31.56 μg mL
MeSH term(s)	Humans ; Staphylococcus aureus ; Biofilms ; Liposomes ; Nanoparticles ; Anti-Bacterial Agents/pharmacology ; Staphylococcal Infections/microbiology ; Bacteria
Chemical Substances	Lipid Nanoparticles ; Liposomes ; Anti-Bacterial Agents
Language	English
Publishing date	2023-12-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2221885-3
ISSN	1860-7314 ; 1860-6768
ISSN (online)	1860-7314
ISSN	1860-6768
DOI	10.1002/biot.202300159
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Zs.A 6349: Show issues

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Article ; Online: Effect and regulation of fatty acids on bacillomycin D synthesis

Ma, Wenjie / Lv, Ziyan / Zhang, Ping / Lu, Zhaoxin / Zheng, Liping / Wang, Zuwei / Zhou, Libang / Meng, Fanqiang / Bie, Xiaomei

World J Microbiol Biotechnol. 2023 May, v. 39, no. 5, p. 113

2023 , Page(s) 113

Abstract: Bacillomycin D is a cyclic antimicrobial lipopeptide that has excellent antifungal effects, but its application is limited due to its low yield. At present, it is not clear whether fatty acids regulate the synthesis of bacillomycin D. Therefore, the ... ...

Abstract	Bacillomycin D is a cyclic antimicrobial lipopeptide that has excellent antifungal effects, but its application is limited due to its low yield. At present, it is not clear whether fatty acids regulate the synthesis of bacillomycin D. Therefore, the effects of nine fatty acids on the yield of bacillomycin D produced by Bacillus amyloliquefaciens fmbJ were studied. The results showed that sodium propionate, propionic acid, and butyric acid could increase the yield of bacillomycin D by 44, 40, and 10%, respectively. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of bacillomycin D synthesis gene, signaling factors and genes related to fatty acid metabolism, so as to explore the mechanism of sodium propionate regulating bacillomycin D synthesis. In conclusion, sodium propionate could accelerate the tricarboxylic acid cycle and promoted spore formation, cell movement, the secretion of extracellular protease and the transcription of bacillomycin D synthesis gene by upregulating the expression of signal factors degU, degQ, sigH, sigM and spo0A and ultimately promoted the synthesis of bacillomycin D. In this study, the mechanism of sodium propionate increasing bacillomycin D production was explored from multiple perspectives, which provided theoretical support for the large-scale production of bacillomycin D and was expected to promote its wide application in food, agriculture and medicine fields.
Keywords	Bacillus amyloliquefaciens ; butyric acid ; cell movement ; fatty acid metabolism ; genes ; lipopeptides ; medicine ; proteinases ; reverse transcriptase polymerase chain reaction ; secretion ; sodium propionate ; spores ; tricarboxylic acid cycle
Language	English
Dates of publication	2023-05
Size	p. 113
Publishing place	Springer Netherlands
Document type	Article ; Online
ZDB-ID	1499109-3
ISSN	1573-0972 ; 0959-3993
ISSN (online)	1573-0972
ISSN	0959-3993
DOI	10.1007/s11274-023-03551-1
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Article ; Online: Construction and optimization of a multiplex PMAxx-qPCR assay for viable Bacillus cereus and development of a detection kit

Hu, Ruirui / Hu, Antuo / Lu, Zhaoxin / Zhou, Haibo / Wei, Wanqing / Lu, Fengxia / Zhao, Haizhen / Bie, Xiaomei

Journal of Microbiological Methods. 2023 Apr., v. 207 p.106705-

2023

Abstract: In this study, a PMAxx-qPCR method for the detection and quantification of viable Bacillus cereus (B. cereus) was established based on the cesA gene that is involved in cereulide synthesis, enterotoxin gene bceT and hemolytic enterotoxin gene hblD ... ...

Abstract	In this study, a PMAxx-qPCR method for the detection and quantification of viable Bacillus cereus (B. cereus) was established based on the cesA gene that is involved in cereulide synthesis, enterotoxin gene bceT and hemolytic enterotoxin gene hblD combined with modified propidium monoazide (PMAxx). The sensitivity detection limit of the method was as follows: the DNA extracted by the kit reached 140 fg/μL, and the bacterial suspension without enrichment reached 2.24 × 10¹ CFU/mL; 14 nonB. cereus strains of the 17 tested strains all tested as negative, whereas the 2 strains of B. cereus carrying the target virulence gene(s) could be accurately detected. In terms of application, we assembled the constructed PMAxx-qPCR reaction into a detection kit and evaluated its application performance. The results showed that the detection kit has high sensitivity, strong anti-interference capability, and has good application potential. The purpose of this study is to provide a reliable detection method for the prevention and traceability of B. cereus infections.
Keywords	Bacillus cereus ; DNA ; detection limit ; enterotoxins ; genes ; propidium ; traceability ; virulence ; PMAxx ; Real-time quantitative PCR ; B. cereus ; Detection kit
Language	English
Dates of publication	2023-04
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2023.106705
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Zs.A 1849: Show issues

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Article: A duplex real-time NASBA assay targeting a serotype-specific gene for rapid detection of viable Salmonella Paratyphi C in retail foods of animal origin

Zhai, Ligong / Liu, Hongxia / Li, Junjie / Lu, Zhaoxin / Bie, Xiaomei

Canadian journal of microbiology. 2022, v. 68, no. 4

2022

Abstract: Salmonella enterica serovar Paratyphi C is highly adapted to humans and can cause a typhoid-like disease with high mortality rates. In this study, three serovar-specific genes were identified by comparative genomics for Salmonella Paratyphi C, SPC_0871, ... ...

Abstract	Salmonella enterica serovar Paratyphi C is highly adapted to humans and can cause a typhoid-like disease with high mortality rates. In this study, three serovar-specific genes were identified by comparative genomics for Salmonella Paratyphi C, SPC_0871, SPC_0872, and SPC_0908. Based on the SPC_0908 and xcd genes for testing Salmonella spp., we developed a duplex real-time nucleic acid sequence-based amplification (real-time NASBA) with a molecular beacon approach for the simultaneous detection of viable cells of Salmonella spp. and serotype Paratyphi C. The test selectively and consistently detected 53 Salmonella spp. (representing 31 serotypes) and 18 non-Salmonella strains. Additionally, the method showed high resistance to interference from natural background flora in pork and chicken samples. The sensitivity of the established approach was determined to be 4.89 cfu/25 g in artificially contaminated pork and chicken samples after pre-enrichment. We propose this NASBA-based protocol as a potential detection method for Salmonella spp. and serotype Paratyphi C in foods of animal origin.
Keywords	Salmonella Paratyphi C ; chickens ; flora ; genes ; genomics ; microbiology ; mortality ; nucleic acids ; pork ; rapid methods ; serotypes
Language	English
Size	p. 259-268.
Publishing place	Canadian Science Publishing
Document type	Article
ZDB-ID	280534-0
ISSN	1480-3275 ; 0008-4166
ISSN (online)	1480-3275
ISSN	0008-4166
DOI	10.1139/cjm-2021-0054
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