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Article ; Online: Patched and Costal-2 mutations lead to differences in tissue overgrowth autonomy

Moore, Shannon L. / Adamini, Frank C. / Coopes, Erik S. / Godoy, Dustin / Northington, Shyra J. / Stewart, Jordan M. / Tillett, Richard L / Bieser, Kayla L. / Kagey, Jacob D.

Fly. 2022 Dec. 31, v. 16, no. 1 p.176-189

2022

Abstract: Genetic screens are used in Drosophila melanogaster to identify genes key in the regulation of organismal development and growth. These screens have defined signalling pathways necessary for tissue and organismal development, which are evolutionarily ... ...

Abstract	Genetic screens are used in Drosophila melanogaster to identify genes key in the regulation of organismal development and growth. These screens have defined signalling pathways necessary for tissue and organismal development, which are evolutionarily conserved across species, including Drosophila. Here, we have used an FLP/FRT mosaic system to screen for conditional regulators of cell growth and cell division in the Drosophila eye. The conditional nature of this screen utilizes a block in the apoptotic pathway to prohibit the mosaic mutant cells from dying via apoptosis. From this screen, we identified two different mutants that mapped to the Hedgehog signalling pathway. Previously, we described a novel Ptc mutation and here we add to the understanding of disrupting the Hh pathway with a novel allele of Cos2. Both of these Hh components are negative regulators of the pathway, yet they depict mutant differences in the type of overgrowth created. Ptc mutations lead to overgrowth consisting of almost entirely wild-type tissue (non-autonomous overgrowth), while the Cos2 mutation results in tissue that is overgrown in both the mutant and wild-type clones (both autonomous and non-autonomous). These differences in tissue overgrowth are consistent in the Drosophila eye and wing. The observed difference is correlated with different deregulation patterns of pMad, the downstream effector of DPP signalling. This finding provides insight into pathway-specific differences that help to better understand intricacies of developmental processes and human diseases that result from deregulated Hedgehog signalling, such as basal cell carcinoma.
Keywords	Drosophila melanogaster ; Erinaceidae ; alleles ; apoptosis ; carcinoma ; cell division ; cell growth ; eyes ; humans ; mutants ; mutation ; genetic screen ; Hedgehog signaling
Language	English
Dates of publication	2022-1231
Size	p. 176-189.
Publishing place	Taylor & Francis
Document type	Article ; Online
ISSN	1933-6942
DOI	10.1080/19336934.2022.2062991
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Chronology, magnitude and duration of expression of putative sex-determining/differentiation genes in a turtle with temperature-dependent sex determination.

Bieser, Kayla L / Wibbels, Thane

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation

2014 Volume 8, Issue 6, Page(s) 364–375

Abstract: The red-eared slider turtle (Trachemys scripta) possesses temperature-dependent sex determination (TSD) in which the incubation temperature determines gonadal sex. Although a number of mammalian gene homologues have been identified in reptiles with TSD, ... ...

Abstract	The red-eared slider turtle (Trachemys scripta) possesses temperature-dependent sex determination (TSD) in which the incubation temperature determines gonadal sex. Although a number of mammalian gene homologues have been identified in reptiles with TSD, the exact sex-determining trigger(s) is not known. To date, the current study represents the most comprehensive simultaneous evaluation of the chronology of mRNA expression profiles of putative sex-determining/differentiation genes (Dmrt1, Sox9, Amh, Lhx9, and Foxl2) from gonads incubated at male- and female-producing temperatures in T. scripta. Additionally, sex-reversing treatments with 17β-estradiol and letrozole were examined. At a male-producing temperature, Dmrt1 expression was sexually dimorphic by stage 17, Sox9 by 19 and Amh by 21. In contrast, Foxl2 did not significantly increase until after the thermosensitive period at a female-producing temperature. Treatment with 17β-estradiol resulted in reduced gonad size and/or inhibited gonadal development and differentiation. Gene expression was subsequently low in this group. Sex reversal utilizing letrozole failed to produce testes at a female-producing temperature and as such, gene expression was comparable to ovary. These results indicate that Dmrt1 and Sox9 are potential triggers for testis differentiation and Amh, Lhx9 and Foxl2 represent a conserved core set of genes in the sex-determining/differentiation pathway of TSD species.
MeSH term(s)	Adrenal Glands/drug effects ; Adrenal Glands/metabolism ; Animals ; Embryo, Nonmammalian/cytology ; Embryo, Nonmammalian/drug effects ; Embryo, Nonmammalian/metabolism ; Estradiol/pharmacology ; Female ; Gene Expression Regulation, Developmental/drug effects ; Gonads/drug effects ; Gonads/embryology ; Gonads/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Male ; Nitriles/pharmacology ; Real-Time Polymerase Chain Reaction ; Sex Determination Processes/drug effects ; Sex Determination Processes/genetics ; Sex Differentiation/drug effects ; Sex Differentiation/genetics ; Temperature ; Time Factors ; Triazoles/pharmacology ; Turtles/embryology ; Turtles/genetics
Chemical Substances	Nitriles ; Triazoles ; Estradiol (4TI98Z838E) ; letrozole (7LKK855W8I)
Language	English
Publishing date	2014
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2253672-3
ISSN	1661-5433 ; 1661-5425
ISSN (online)	1661-5433
ISSN	1661-5425
DOI	10.1159/000369116
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Article ; Online: Patched

Moore, Shannon L / Adamini, Frank C / Coopes, Erik S / Godoy, Dustin / Northington, Shyra J / Stewart, Jordan M / Tillett, Richard L / Bieser, Kayla L / Kagey, Jacob D

Fly

2022 Volume 16, Issue 1, Page(s) 176–189

Abstract: Genetic screens are used ... ...

Abstract	Genetic screens are used in
MeSH term(s)	Animals ; Drosophila/genetics ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/metabolism ; Hedgehog Proteins/genetics ; Hedgehog Proteins/metabolism ; Mutation ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism
Chemical Substances	Drosophila Proteins ; Hedgehog Proteins ; Receptors, Cell Surface ; dpp protein, Drosophila
Language	English
Publishing date	2022-02-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1933-6942
ISSN (online)	1933-6942
DOI	10.1080/19336934.2022.2062991
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Article: Chronology, Magnitude and Duration of Expression of Putative Sex-Determining/Differentiation Genes in a Turtle with Temperature-Dependent Sex Determination

Bieser, Kayla L. / Wibbels, Thane

Sexual Development

2014 Volume 8, Issue 6, Page(s) 364–375

Institution	Department of Biology, University of Alabama at Birmingham, Birmingham, Ala., and Department of Biology, Northland College, Ashland, Wis., USA
Abstract	The red-eared slider turtle (Trachemys scripta) possesses temperature-dependent sex determination (TSD) in which the incubation temperature determines gonadal sex. Although a number of mammalian gene homologues have been identified in reptiles with TSD, the exact sex-determining trigger(s) is not known. To date, the current study represents the most comprehensive simultaneous evaluation of the chronology of mRNA expression profiles of putative sex-determining/differentiation genes (Dmrt1Sox9AmhLhx9, and Foxl2) from gonads incubated at male- and female-producing temperatures in T. scripta. Additionally, sex-reversing treatments with 17β-estradiol and letrozole were examined. At a male-producing temperature, Dmrt1 expression was sexually dimorphic by stage 17, Sox9 by 19 and Amh by 21. In contrast, Foxl2 did not significantly increase until after the thermosensitive period at a female-producing temperature. Treatment with 17β-estradiol resulted in reduced gonad size and/or inhibited gonadal development and differentiation. Gene expression was subsequently low in this group. Sex reversal utilizing letrozole failed to produce testes at a female-producing temperature and as such, gene expression was comparable to ovary. These results indicate that Dmrt1 and Sox9 are potential triggers for testis differentiation and Amh, Lhx9 and Foxl2 represent a conserved core set of genes in the sex-determining/differentiation pathway of TSD species.
Keywords	17β-estradiol ; Letrozole ; TSD
Language	English
Publishing date	2014-11-25
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Original Article
ZDB-ID	2253672-3
ISSN	1661-5433 ; 1661-5425
ISSN (online)	1661-5433
ISSN	1661-5425
DOI	10.1159/000369116
Database	Karger publisher's database

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Article: Fly-CURE, a multi-institutional CURE using

Merkle, Julie A / Devergne, Olivier / Kelly, Seth M / Croonquist, Paula A / Evans, Cory J / Hwalek, Melanie A / Straub, Victoria L / Hamill, Danielle R / Peister, Alexandra / Puthoff, David P / Saville, Ken J / Siders, Jamie L / Villanueva Gonzalez, Zully J / Wittke-Thompson, Jacqueline K / Bieser, Kayla L / Stamm, Joyce / Vrailas-Mortimer, Alysia D / Kagey, Jacob D

Journal of microbiology & biology education

2023 Volume 24, Issue 3

Abstract: The Fly-CURE is a genetics-focused multi-institutional Course-Based Undergraduate Research Experience (CURE) that provides undergraduate students with hands-on research experiences within a course. Through the Fly-CURE, undergraduate students at diverse ... ...

Abstract	The Fly-CURE is a genetics-focused multi-institutional Course-Based Undergraduate Research Experience (CURE) that provides undergraduate students with hands-on research experiences within a course. Through the Fly-CURE, undergraduate students at diverse types of higher education institutions across the United States map and characterize novel mutants isolated from a genetic screen in
Language	English
Publishing date	2023-09-21
Publishing country	United States
Document type	Journal Article
ISSN	1935-7877
ISSN	1935-7877
DOI	10.1128/jmbe.00245-22
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Article: Fly-CURE, a Multi-institutional CURE using

Merkle, Julie A / Devergne, Olivier / Kelly, Seth M / Croonquist, Paula A / Evans, Cory J / Hwalek, Melanie A / Straub, Victoria L / Hamill, Danielle R / Puthoff, David P / Saville, Kenneth J / Siders, Jamie L / Gonzalez, Zully J Villanueva / Wittke-Thompson, Jackie K / Bieser, Kayla L / Stamm, Joyce / Vrailas-Mortimer, Alysia D / Kagey, Jacob D

bioRxiv : the preprint server for biology

2023

Abstract	The Fly-CURE is a genetics-focused multi-institutional Course-Based Undergraduate Research Experience (CURE) that provides undergraduate students with hands-on research experiences within a course. Through the Fly-CURE, undergraduate students at diverse types of higher education institutions across the United States map and characterize novel mutants isolated from a genetic screen in
Language	English
Publishing date	2023-01-19
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.01.16.524319
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The cloning and expression analysis of Lhx9 during gonadal sex differentiation in the red-eared slider turtle, Trachemys scripta, a species with temperature-dependent sex determination.

Bieser, Kayla L / Wibbels, Thane / Mourad, George / Paladino, Frank

Journal of experimental zoology. Part B, Molecular and developmental evolution

2013 Volume 320, Issue 4, Page(s) 238–246

Abstract: Many reptiles, including the red-eared slider turtle (Trachemys scripta), possess a temperature-dependent sex determination (TSD) mechanism where the temperature at which the developing embryos are incubated dictates the gonadal sex of the animal. A ... ...

Abstract	Many reptiles, including the red-eared slider turtle (Trachemys scripta), possess a temperature-dependent sex determination (TSD) mechanism where the temperature at which the developing embryos are incubated dictates the gonadal sex of the animal. A number of mammalian gene orthologues have been identified in the sex determination/differentiation cascade of reptiles with TSD although the exact trigger(s) is not well understood. A potential early regulator of gonadal differentiation, Lhx9, controls the proliferation of gonadal cells in mice and its absence prevents gonadal development and drastically reduces the expression of Sf-1, a gene that regulates the expression of steroidogenic enzymes in the bipotential gonad. In the current study, we cloned Lhx9 from T. scripta and analyzed its expression throughout the thermosensitive period of gonad development using quantitative PCR. We examined the expression profiles of Lhx9 in embryos incubated under control conditions at male- and female-producing temperatures and with the application of exogenous 17β-estradiol or an aromatase inhibitor, Letrozole, to induce sex reversal. The T. scripta Lhx9 cDNA and predicted amino acid sequence showed high homology to those of chicken, anole, and mouse. Lhx9 was expressed at both male- and female-producing temperatures with expression levels increasing throughout the thermosensitive period. Letrozole induced sex-reversal did not alter Lhx9 expression levels. 17β-estradiol treatments appeared to inhibit or delay gonadal differentiation and resulted in lower Lhx9 expression from the presumptive gonadal ridge region. The structural homology and temporal expression pattern of Lhx9 suggests that this represents a conserved element in the gonadal differentiation cascade of T. scripta.
MeSH term(s)	Animals ; Aromatase Inhibitors/pharmacology ; Cloning, Molecular ; Female ; Gene Expression Regulation, Developmental/drug effects ; Gonads/cytology ; Gonads/growth & development ; LIM-Homeodomain Proteins/genetics ; Male ; Sex Determination Processes/drug effects ; Sex Determination Processes/genetics ; Sex Differentiation ; Temperature ; Transcription Factors/genetics ; Turtles/genetics ; Turtles/growth & development
Chemical Substances	Aromatase Inhibitors ; LIM-Homeodomain Proteins ; Lhx9 protein, mouse ; Transcription Factors
Language	English
Publishing date	2013-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2103823-5
ISSN	1552-5015 ; 0022-104X ; 1552-5007
ISSN (online)	1552-5015
ISSN	0022-104X ; 1552-5007
DOI	10.1002/jez.b.22497
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Article ; Online: The

Evans, Cory J / Bieser, Kayla L / Acevedo-Vasquez, Katherine S / Augustine, Emyli J / Bowen, Skyler / Casarez, Veronica A / Feliciano, Vanessa I / Glazier, Ashley / Guinan, Haley R / Hallman, Randy / Haugan, Elizabeth / Hehr, Lauren A / Hunnicutt, Shawna N / Leifer, Isabella / Mauger, Meaghan / Mauger, Morgan / Melendez, Norma Y / Milshteyn, Larry / Moore, Eric /

Nguyen, Sarah A / Phanphouvong, Sierra C / Pinal, David M / Pope, Hailee M / Salinas, Mark-Brandon M / Shellin, Matthew / Small, Ivana / Yeoh, Neelufar C / Yokomizo, Alexandra M K / Kagey, Jacob D

microPublication biology

2022 Volume 2022

Abstract: ... The ... ...

Abstract	The mutation
Language	English
Publishing date	2022-10-25
Publishing country	United States
Document type	Journal Article
ISSN	2578-9430
ISSN (online)	2578-9430
DOI	10.17912/micropub.biology.000653
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Article ; Online: Genetic mapping of the

Cordes, Chloe N / Gouge, Melissa M / Morgan, Hannah / Porter, Carah / Siders, Jamie / Bacab, Erica / Barin, Briana / Becerra, Ixel A / Castillo, Jose / Church, Jared / Condo-Hicks, Shaunacee / Conroy, Kattleya J / Curbelo-Navarro, Isbel / DePrest, Benjamin J / Eady, Adrienne / Edmead, TyMahni / Farouk, Verena / Flores-Torres, Angelica / Girmai, Michael /

microPublication biology

2023 Volume 2023

Abstract: An EMS-based forward genetic screen was conducted in an apoptotic null background to identify genetic aberrations that contribute to regulation of cell growth ... ...

Abstract	An EMS-based forward genetic screen was conducted in an apoptotic null background to identify genetic aberrations that contribute to regulation of cell growth in
Language	English
Publishing date	2023-09-18
Publishing country	United States
Document type	Journal Article
ISSN	2578-9430
ISSN (online)	2578-9430
DOI	10.17912/micropub.biology.000783
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Genetic mapping and phenotypic analysis of

Talley, Elyse M / Watts, Charlie T / Aboyer, Sonia / Adamson, Madeline G / Akoto, Harriet Ab / Altemus, Haley / Avella, Philip J / Bailey, Rebecca / Bell, Elizabeth R / Bell, Katheryn L / Breneman, Kelsey / Burkhart, Jessica S / Chanley, Logan J / Cook, Savannah S / DesLaurier, Mackenzie T / Dorsey, Timothy R / Doyle, Cassandra J / Egloff, Merris E / Fasawe, Ayoola S /

Garcia, Katy K / Graves, Nathaniel P / Gray, Tyler K / Gustafson, Evan M / Hall, Makayla J / Hayes, Jaden D / Holic, Lindsay J / Jarvis, Brice A / Klos, Piotr S / Kritzmire, Sidney / Kuzovko, Lera / Lainez, Edwyna / McCoy, Shamerra / Mierendorf, James C / Neri, Nicole A / Neville, Caley R / Osborn, Kelley / Parker, Kaitlyn / Parks, Megan E / Peck, Kylee / Pitt, Robyn / Platta, Matthew E / Powell, Brianna / Rodriguez, Katalina / Ruiz, Clara / Schaefer, Mariah N / Shields, Amanda B / Smiley, Jasmine B / Stauffer, Briona / Straub, Devan / Sweeney, John L / Termine, Kaitlyn M / Thomas, Brett / Toth, Sophia D / Veile, Taylor R / Walker, Kayla S / Webster, Paige N / Woodard, Brian J / Yoder, Quentin L / Young, McKenzie K / Zeedyk, McKenzie L / Ziegler, Logan N / Bieser, Kayla L / Puthoff, David P / Stamm, Joyce / Vrailas-Mortimer, Alysia D / Kagey, Jacob D / Merkle, Julie A

microPublication biology

2021 Volume 2021

Abstract: Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen ... ...

Abstract	Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in
Language	English
Publishing date	2021-07-13
Publishing country	United States
Document type	Journal Article
ISSN	2578-9430
ISSN (online)	2578-9430
DOI	10.17912/micropub.biology.000418
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