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  1. Article ; Online: Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line

    Ayse Banu Demir / Domenico Benvenuto / Bilge Karacicek / Yasemin Erac / Silvia Spoto / Silvia Angeletti / Massimo Ciccozzi / Metiner Tosun

    Journal of Personalized Medicine, Vol 12, Iss 2065, p

    2022  Volume 2065

    Abstract: Elevated levels of STIM1, an endoplasmic reticulum Ca 2+ sensor/buffering protein, appear to be correlated with poor cancer prognosis in which microRNAs are also known to play critical roles. The purpose of this study is to investigate possible HBV ... ...

    Abstract Elevated levels of STIM1, an endoplasmic reticulum Ca 2+ sensor/buffering protein, appear to be correlated with poor cancer prognosis in which microRNAs are also known to play critical roles. The purpose of this study is to investigate possible HBV origins of specific microRNAs we identified in a stem cell-like subpopulation of Huh-7 hepatocellular carcinoma (HCC) cell lines with enhanced STIM1 and/or Orai1 expression that mimicked poor cancer prognosis. Computational strategies including phylogenetic analyses were performed on miRNome data we obtained from an EpCAM- and CD133-expressing Huh-7 HCC stem cell-like subpopulation with enhanced STIM1 and/or Orai1 expression originally cultured in the present work. Results revealed two putative regions in the HBV genome based on the apparent clustering pattern of stem loop sequences of microRNAs, including miR3653. Reciprocal analysis of these regions identified critical human genes, of which their transcripts are among the predicted targets of miR3653, which was increased significantly by STIM1 or Orai1 enhancement. Briefly, this study provides phylogenetic evidence for a possible HBV-driven epigenetic remodeling that alters the expression pattern of Ca 2+ homeostasis-associated genes in STIM1 - or Orai1 overexpressing liver cancer stem-like cells for a possible mutual survival outcome. A novel region on HBV-X protein may affect liver carcinogenesis in a genotype-dependent manner. Therefore, detection of the viral genotype would have a clinical impact on prognosis of HBV-induced liver cancers.
    Keywords hepatocellular carcinoma ; HBx ; miR3653 ; epithelial–mesenchymal transition (EMT) ; molecular evolution ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Electrochemical detection of interaction between capsaicin and nucleic acids in comparison to agarose gel electrophoresis

    Yilmaz, Nilay / Ece Eksin / Bilge Karacicek / Yasemin Eraç / Arzum Erdem

    Analytical biochemistry. 2017 Oct. 15, v. 535

    2017  

    Abstract: In this study, the biomolecular interaction occurred between nucleic acids and Capsaicin (CPS), the active compound in chilli peppers, which has been reported to have anti-carcinogenic properties, was investigated for the first time herein using ... ...

    Abstract In this study, the biomolecular interaction occurred between nucleic acids and Capsaicin (CPS), the active compound in chilli peppers, which has been reported to have anti-carcinogenic properties, was investigated for the first time herein using disposable electrochemical biosensor. It is aimed to perform the surface-confined interaction between CPS and different types of nucleic acids and under this aim, the experimental conditions were optimized; such as, the concentration of CPS and DNA, DNA immobilization time and interaction time etc. The detection limit of DNA was estimated based on guanine oxidation signal in the linear concentration range of DNA from 1 to 5 μg/mL, and it was found to be 0.62 μg/mL. The effect of time-dependent manner from 1 min to 30 min on the interaction of CPS with nucleic acids was explored upon to the changes at guanine signal coming from double stranded DNA and cDNA as well as PCR samples. The interaction of CPS with double stranded DNA was also determined by agarose gel electrophoresis.
    Keywords active ingredients ; agar gel electrophoresis ; anticarcinogenic activity ; biosensors ; capsaicin ; complementary DNA ; electrochemistry ; guanine ; hot peppers ; oxidation ; polymerase chain reaction
    Language English
    Dates of publication 2017-1015
    Size p. 56-62.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2017.07.023
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: A Novel Function for KLF4 in Modulating the De-differentiation of EpCAM - /CD133 - nonStem Cells into EpCAM + /CD133 + Liver Cancer Stem Cells in HCC Cell Line HuH7

    Zeynep Firtina Karagonlar / Soheil Akbari / Mustafa Karabicici / Eren Sahin / Sanem Tercan Avci / Nevin Ersoy / Kıvılcım Eren Ates / Tugsan Balli / Bilge Karacicek / Kubra Nur Kaplan / Canan Celiker / Nese Atabey / Esra Erdal

    Cells, Vol 9, Iss 1198, p

    2020  Volume 1198

    Abstract: The complex and heterogeneous nature of hepatocellular carcinoma (HCC) hampers the identification of effective therapeutic strategies. Cancer stem cells (CSCs) represent a fraction of cells within tumors with the ability to self-renew and differentiate, ... ...

    Abstract The complex and heterogeneous nature of hepatocellular carcinoma (HCC) hampers the identification of effective therapeutic strategies. Cancer stem cells (CSCs) represent a fraction of cells within tumors with the ability to self-renew and differentiate, and thus significantly contribute to the formation and maintenance of heterogeneous tumor mass. Increasing evidence indicates high plasticity in tumor cells, suggesting that non-CSCs could acquire stem cell properties through de-differentiation or reprogramming processes. In this paper, we reveal KLF4 as a transcription factor that can induce a CSC-like phenotype in non-CSCs through upregulating the EpCAM and E-CAD expression. Our studies indicated that KLF4 could directly bind to the promoter of EpCAM and increase the number of EpCAM + /CD133 + liver cancer stem cells (LCSCs) in the HuH7 HCC cell line. When KLF4 was overexpressed in EpCAM - /CD133 - non-stem cells, the expressions of hepatic stem/progenitor cell genes such as CK19 , EpCAM and LGR5 were significantly increased. KLF4 overexpressing non-stem cells exhibited greater cell viability upon sorafenib treatment, while the cell migration and invasion capabilities of these cells were suppressed. Importantly, we detected an increased membranous expression and colocalization of β-CAT, E-CAD and EpCAM in the KLF4-overexpressing EpCAM - /CD133 - non-stem cells, suggesting that this complex might be required for the cancer stem cell phenotype . Moreover , our in vivo xenograft studies demonstrated that with a KLF4 overexpression, EpCAM - /CD133 - non-stem cells attained an in vivo tumor forming ability comparable to EpCAM + /CD133 + LCSCs, and the tumor specimens from KLF4-overexpressing xenografts had increased levels of both the KLF4 and EpCAM proteins. Additionally, we identified a correlation between the KLF4 and EpCAM protein expressions in human HCC tissues independent of the tumor stage and differentiation status. Collectively, our data suggest a novel function for KLF4 in modulating the ...
    Keywords hepatocellular carcinoma (HCC) ; liver cancer stem cells ; tumor plasticity ; KLF4 ; reprogramming ; EpCAM ; Biology (General) ; QH301-705.5
    Subject code 333
    Language English
    Publishing date 2020-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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