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  1. Article ; Online: Open-Spaced Ridged Hydrogel Scaffolds Containing TiO 2 -Self-Assembled Monolayer of Phosphonates Promote Regeneration and Recovery Following Spinal Cord Injury

    Ahad M. Siddiqui / Frederic Thiele / Rachel N. Stewart / Simone Rangnick / Georgina J. Weiss / Bingkun K. Chen / Jodi L. Silvernail / Tammy Strickland / Jarred J. Nesbitt / Kelly Lim / Jean E. Schwarzbauer / Jeffrey Schwartz / Michael J. Yaszemski / Anthony J. Windebank / Nicolas N. Madigan

    International Journal of Molecular Sciences, Vol 24, Iss 10250, p

    2023  Volume 10250

    Abstract: The spinal cord has a poor ability to regenerate after an injury, which may be due to cell loss, cyst formation, inflammation, and scarring. A promising approach to treating a spinal cord injury (SCI) is the use of biomaterials. We have developed a novel ...

    Abstract The spinal cord has a poor ability to regenerate after an injury, which may be due to cell loss, cyst formation, inflammation, and scarring. A promising approach to treating a spinal cord injury (SCI) is the use of biomaterials. We have developed a novel hydrogel scaffold fabricated from oligo(poly(ethylene glycol) fumarate) (OPF) as a 0.08 mm thick sheet containing polymer ridges and a cell-attractive surface on the other side. When the cells are cultured on OPF via chemical patterning, the cells attach, align, and deposit ECM along the direction of the pattern. Animals implanted with the rolled scaffold sheets had greater hindlimb recovery compared to that of the multichannel scaffold control, which is likely due to the greater number of axons growing across it. The immune cell number (microglia or hemopoietic cells: 50–120 cells/mm 2 in all conditions), scarring (5–10% in all conditions), and ECM deposits (Laminin or Fibronectin: approximately 10–20% in all conditions) were equal in all conditions. Overall, the results suggest that the scaffold sheets promote axon outgrowth that can be guided across the scaffold, thereby promoting hindlimb recovery. This study provides a hydrogel scaffold construct that can be used in vitro for cell characterization or in vivo for future neuroprosthetics, devices, or cell and ECM delivery.
    Keywords axon regeneration ; biomaterials ; immune cells ; machine learning ; mesenchymal stromal cells (MSCs) ; oligo(poly(ethylene glycol)) fumarate ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 571
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Newly regenerated axons via scaffolds promote sub-lesional reorganization and motor recovery with epidural electrical stimulation

    Ahad M. Siddiqui / Riazul Islam / Carlos A. Cuellar / Jodi L. Silvernail / Bruce Knudsen / Dallece E. Curley / Tammy Strickland / Emilee Manske / Parita T. Suwan / Timur Latypov / Nafis Akhmetov / Shuya Zhang / Priska Summer / Jarred J. Nesbitt / Bingkun K. Chen / Peter J. Grahn / Nicolas N. Madigan / Michael J. Yaszemski / Anthony J. Windebank /
    Igor A. Lavrov

    npj Regenerative Medicine, Vol 6, Iss 1, Pp 1-

    2021  Volume 12

    Abstract: Abstract Here, we report the effect of newly regenerated axons via scaffolds on reorganization of spinal circuitry and restoration of motor functions with epidural electrical stimulation (EES). Motor recovery was evaluated for 7 weeks after spinal ... ...

    Abstract Abstract Here, we report the effect of newly regenerated axons via scaffolds on reorganization of spinal circuitry and restoration of motor functions with epidural electrical stimulation (EES). Motor recovery was evaluated for 7 weeks after spinal transection and following implantation with scaffolds seeded with neurotrophin producing Schwann cell and with rapamycin microspheres. Combined treatment with scaffolds and EES-enabled stepping led to functional improvement compared to groups with scaffold or EES, although, the number of axons across scaffolds was not different between groups. Re-transection through the scaffold at week 6 reduced EES-enabled stepping, still demonstrating better performance compared to the other groups. Greater synaptic reorganization in the presence of regenerated axons was found in group with combined therapy. These findings suggest that newly regenerated axons through cell-containing scaffolds with EES-enabled motor training reorganize the sub-lesional circuitry improving motor recovery, demonstrating that neuroregenerative and neuromodulatory therapies cumulatively enhancing motor function after complete SCI.
    Keywords Medicine ; R
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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