LIVIVO - Search results -

Search results

Result 1 - 10 of total 174

Search options

Article ; Online: Exosome Traceability and Cell Source Dependence on Composition and Cell-Cell Cross Talk.

Hamzah, Rabab N / Alghazali, Karrer M / Biris, Alexandru S / Griffin, Robert J

International journal of molecular sciences

2021 Volume 22, Issue 10

Abstract: Exosomes are small vesicles with an average diameter of 100 nm that are produced by many, if not all, cell types. Exosome cargo includes lipids, proteins, and nucleic acids arranged specifically in the endosomes of donor cells. Exosomes can transfer the ... ...

Abstract	Exosomes are small vesicles with an average diameter of 100 nm that are produced by many, if not all, cell types. Exosome cargo includes lipids, proteins, and nucleic acids arranged specifically in the endosomes of donor cells. Exosomes can transfer the donor cell components to target cells and can affect cell signaling, proliferation, and differentiation. Important new information about exosomes' remote communication with other cells is rapidly being accumulated. Recent data indicates that the results of this communication depend on the donor cell type and the environment of the host cell. In the field of cancer research, major questions remain, such as whether tumor cell exosomes are equally taken up by cancer cells and normal cells and whether exosomes secreted by normal cells are specifically taken up by other normal cells or also tumor cells. Furthermore, we do not know how exosome uptake is made selective, how we can trace exosome uptake selectivity, or what the most appropriate methods are to study exosome uptake and selectivity. This review will explain the effect of exosome source and the impact of the donor cell growth environment on tumor and normal cell interaction and communication. The review will also summarize the methods that have been used to label and trace exosomes to date.
MeSH term(s)	Biological Transport/physiology ; Cell Communication/physiology ; Cell Line ; Cell Line, Tumor ; Cell Proliferation/physiology ; Endosomes/physiology ; Exosomes/metabolism ; Exosomes/physiology ; Humans ; Signal Transduction/physiology
Language	English
Publishing date	2021-05-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22105346
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Cell-Biomaterial constructs for wound healing and skin regeneration.

Safina, Ingrid / Childress, Luke T / Myneni, Srinivas R / Vang, Kieng Bao / Biris, Alexandru S

Drug metabolism reviews

2022 Volume 54, Issue 1, Page(s) 63–94

Abstract: Over the years, conventional skin grafts, such as full-thickness, split-thickness, and pre-sterilized grafts from human or animal sources, have been at the forefront of skin wound care. However, these conventional grafts are associated with major ... ...

Abstract	Over the years, conventional skin grafts, such as full-thickness, split-thickness, and pre-sterilized grafts from human or animal sources, have been at the forefront of skin wound care. However, these conventional grafts are associated with major challenges, including supply shortage, rejection by the immune system, and disease transmission following transplantation. Due to recent progress in nanotechnology and material sciences, advanced artificial skin grafts-based on the fundamental concepts of tissue engineering-are quickly evolving for wound healing and regeneration applications, mainly because they can be uniquely tailored to meet the requirements of specific injuries. Despite tremendous progress in tissue engineering, many challenges and uncertainties still face skin grafts
MeSH term(s)	Animals ; Biocompatible Materials ; Humans ; Skin ; Skin Transplantation ; Skin, Artificial ; Tissue Engineering ; Wound Healing
Chemical Substances	Biocompatible Materials
Language	English
Publishing date	2022-04-02
Publishing country	England
Document type	Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	184967-0
ISSN	1097-9883 ; 0360-2532 ; 0012-6594
ISSN (online)	1097-9883
ISSN	0360-2532 ; 0012-6594
DOI	10.1080/03602532.2021.2025387
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1030: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Cellular Uptake of Gold Nanorods in Breast Cancer Cell Lines.

White, Bryan E / White, Molly K / Nima Alsudani, Zeid A / Watanabe, Fumiya / Biris, Alexandru S / Ali, Nawab

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 6

Abstract: Nanosized materials have been proposed for a wide range of biomedical applications, given their unique characteristics. However, how these nanomaterials interact with cells and tissues, as well as how they bio-distribute in organisms, is still under ... ...

Abstract	Nanosized materials have been proposed for a wide range of biomedical applications, given their unique characteristics. However, how these nanomaterials interact with cells and tissues, as well as how they bio-distribute in organisms, is still under investigation. Differences such as the nanoparticle size, shape, and surface chemistry affect the basic mechanisms of cellular uptake and responses, which, in turn, affects the nanoparticles' applicability for biomedical applications. Thus, it is vital to determine how a specific nanoparticle interacts with cells of interest before extensive
Language	English
Publishing date	2022-03-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12060937
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Plasmonic gap-enhanced Raman tag nanorods for imaging 3D pancreatic spheroids using surface-enhanced Raman spectroscopy and darkfield microscopy.

Darrigues, Emilie / Nima Al Sudani, Zeid A / Watanabe, Fumiya / Biris, Alexandru S

Nanotechnology

2020 Volume 32, Issue 9, Page(s) 95104

Abstract: Plasmonic gap-enhanced Raman tags (GERTs) are new emerging nanoprobes that, based on their unique surface-enhanced Raman spectroscopy (SERS) signal, can play a major role in complex imaging and detection of biological systems. GERTs are generated from a ... ...

Abstract	Plasmonic gap-enhanced Raman tags (GERTs) are new emerging nanoprobes that, based on their unique surface-enhanced Raman spectroscopy (SERS) signal, can play a major role in complex imaging and detection of biological systems. GERTs are generated from a metal core nanostructure and layered with one or more metal nanosized layers, encasing a Raman active molecule. The advantages of GERTs are enhanced surface plasmon and electromagnetic resonance, as well as inherent protection of the Raman active molecule from environmental deterioration that could reduce their spectroscopic signatures over time. In this study, we used in vitro three-dimensional (3D) spheroid cultures to demonstrate these advantages. 3D spheroids mimic the in vivo tumor microenvironment better than 2D culture, with abundant extracellular matrix and hypoxia inducing variability of pH and enzymatic reactions. Here, we report the use of GERTs in large pancreatic 3D spheroids (>500 μm in apparent diameter) for complex penetration visualization. Our combined imaging technique of enhanced darkfield microscopy and SERS was able to identify the presence and distribution of the GERTs within the 3D spheroid structure. The distribution of GERTs 2 hours after the nanorods' incubation indicated accumulation, generally in the outermost layer of the spheroids but also, more randomly, in non-uniform patterns in deep layers of the 3D spheroids. These observations bring into question the mechanism of uptake and flow of the nanoparticles in function of their incubation time while demonstrating the promising potential of our approach. Additionally, the SERS signal was still detectable after 24 hours of incubation of GERTs with the 3D culture, indicating the stability of the Raman signal.
Language	English
Publishing date	2020-12-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1362365-5
ISSN	1361-6528 ; 0957-4484
ISSN (online)	1361-6528
ISSN	0957-4484
DOI	10.1088/1361-6528/abc643
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Dynamic blood flow phantom with negative and positive photoacoustic contrasts.

Jawad, Hind J / Sarimollaoglu, Mustafa / Biris, Alexandru S / Zharov, Vladimir P

Biomedical optics express

2018 Volume 9, Issue 10, Page(s) 4702–4713

Abstract: ... In ... ...

Abstract	In vivo
Language	English
Publishing date	2018-09-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.9.004702
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: 3D cultures for modeling nanomaterial-based photothermal therapy.

Darrigues, Emilie / Nima, Zeid A / Griffin, Robert J / Anderson, James M / Biris, Alexandru S / Rodriguez, Analiz

Nanoscale horizons

2019 Volume 5, Issue 3, Page(s) 400–430

Abstract: Photothermal therapy (PTT) is one of the most promising techniques for cancer tumor ablation. Nanoparticles are increasingly being investigated for use with PTT and can serve as theranostic agents. Based on the ability of near-infrared nano-photo- ... ...

Abstract	Photothermal therapy (PTT) is one of the most promising techniques for cancer tumor ablation. Nanoparticles are increasingly being investigated for use with PTT and can serve as theranostic agents. Based on the ability of near-infrared nano-photo-absorbers to generate heat under laser irradiation, PTT could prove advantageous in certain situations over more classical cancer therapies. To analyze the efficacy of nanoparticle-based PTT, preclinical in vitro studies typically use 2D cultures, but this method cannot completely mimic the complex tumor organization, bioactivity, and physiology that all control the complex penetration depth, biodistribution, and tissue diffusion parameters of nanomaterials in vivo. To fill this knowledge gap, 3D culture systems have been explored for PTT analysis. These models provide more realistic microenvironments that allow spatiotemporal oxygen gradients and cancer cell adaptations to be considered. This review highlights the work that has been done to advance 3D models for cancer microenvironment modeling, specifically in the context of advanced, functionalized nanoparticle-directed PTT.
MeSH term(s)	Cell Culture Techniques/methods ; Cell Line, Tumor ; Humans ; Hyperthermia, Induced/methods ; Infrared Rays/therapeutic use ; Lasers ; Nanostructures/therapeutic use ; Phototherapy/methods ; Spheroids, Cellular ; Theranostic Nanomedicine/methods ; Tumor Microenvironment
Language	English
Publishing date	2019-11-29
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ISSN	2055-6764
ISSN (online)	2055-6764
DOI	10.1039/c9nh00628a
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Influence of a novel scaffold composed of polyurethane, hydroxyapatite, and decellularized bone particles on the healing of fourth metacarpal defects in mares

Grzeskowiak, Remigiusz M. / Alghazali, Karrer M. / Hecht, Silke / Donnell, Robert L. / Doherty, Thomas J. / Smith, Christopher K. / Anderson, David E. / Biris, Alexandru S. / Adair, Henry S.

Veterinary surgery. 2021 July, v. 50, no. 5

2021

Abstract: OBJECTIVE: To determine the effect of a novel scaffold, designed for use in bone regeneration, on healing of splint bone segmental defects in mares. STUDY DESIGN: In vivo experimental study. SAMPLE POPULATION: Five adult mares (4–10 years old; mean ... ...

Abstract	OBJECTIVE: To determine the effect of a novel scaffold, designed for use in bone regeneration, on healing of splint bone segmental defects in mares. STUDY DESIGN: In vivo experimental study. SAMPLE POPULATION: Five adult mares (4–10 years old; mean weight, 437.7 kg ± 29 kg). METHODS: Bilateral 2‐cm full‐thickness defects were created in the fourth metacarpal bones (MCIV) of each horse. Each defect was randomly assigned to either a novel scaffold treatment (n = 5) or an untreated control (n = 5). The scaffold was composed of polyurethane, hydroxyapatite, and decellularized bone particles. Bone healing was assessed for a period of 60 days by thermography, ultrasonography, radiography, and computed tomography (CT). Biopsies of each defect were performed 60 days after surgery for histological evaluation. RESULTS: On the basis of radiographic analysis, scaffold‐treated defects had greater filling (67.42% ± 26.7%) compared with untreated defects (35.88% ± 32.7%; P = .006). After 60 days, CT revealed that the density of the defects treated with the scaffolds (807.80 ± 129.6 Hounsfield units [HU]) was greater than density of the untreated defects (464.80 ± 81.3 HU; P = .004). Evaluation of histology slides provided evidence of bone formation within an average of 9.43% ± 3.7% of the cross‐sectional area of scaffolds in contrast to unfilled defects in which connective tissue was predominant throughout the biopsy specimens. CONCLUSION: The novel scaffold was biocompatible and supported bone formation within the MCIV segmental defects. CLINICAL SIGNIFICANCE: This novel scaffold offers an effective option for filling bone voids in horses when support of bone healing is indicated.
Keywords	adults ; biopsy ; bone formation ; computed tomography ; histology ; hydroxyapatite ; metacarpus ; polyurethanes ; radiography ; thermography ; ultrasonography
Language	English
Dates of publication	2021-07
Size	p. 1117-1127.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1491071-8
ISSN	1532-950X ; 0161-3499
ISSN (online)	1532-950X
ISSN	0161-3499
DOI	10.1111/vsu.13608
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Towards rainbow portable Cytophone with laser diodes for global disease diagnostics.

Jawad, Hind J / Yadem, Aayire C / Menyaev, Yulian A / Sarimollaoglu, Mustafa / Armstrong, Jillian N / Watanabe, Fumiya / Biris, Alexandru S / Stumhofer, Jason S / Nedosekin, Dmitry / Suen, James Y / Parikh, Sunil / Zharov, Vladimir P

Scientific reports

2022 Volume 12, Issue 1, Page(s) 8671

Abstract: In vivo, Cytophone has demonstrated the capability for the early diagnosis of cancer, infection, and cardiovascular disorders through photoacoustic detection of circulating disease markers directly in the bloodstream with an unprecedented 1,000-fold ... ...

Abstract	In vivo, Cytophone has demonstrated the capability for the early diagnosis of cancer, infection, and cardiovascular disorders through photoacoustic detection of circulating disease markers directly in the bloodstream with an unprecedented 1,000-fold improvement in sensitivity. Nevertheless, a Cytophone with higher specificity and portability is urgently needed. Here, we introduce a novel Cytophone platform that integrates a miniature multispectral laser diode array, time-color coding, and high-speed time-resolved signal processing. Using two-color (808 nm/915 nm) laser diodes, we demonstrated spectral identification of white and red clots, melanoma cells, and hemozoin in malaria-infected erythrocytes against a blood background and artifacts. Data from a Plasmodium yoelii murine model and cultured human P. falciparum were verified in vitro with confocal photothermal and fluorescent microscopy. With these techniques, we detected infected cells within 4 h after invasion, which makes hemozoin promising as a spectrally selective marker at the earliest stages of malaria progression. Along with the findings from our previous application of Cytophone with conventional lasers for the diagnosis of melanoma, bacteremia, sickle anemia, thrombosis, stroke, and abnormal hemoglobin forms, this current finding suggests the potential for the development of a portable rainbow Cytophone with multispectral laser diodes for the identification of these and other diseases.
MeSH term(s)	Animals ; Early Detection of Cancer ; Erythrocytes ; Lasers, Semiconductor ; Malaria/diagnosis ; Melanoma ; Mice ; Plasmodium falciparum ; Plasmodium yoelii
Language	English
Publishing date	2022-05-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-11452-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes.

Sharma, Krishna Deo / Alghazali, Karrer M / Hamzah, Rabab N / Pandanaboina, Sahitya Chetan / Nima Alsudani, Zeid A / Muhi, Malek / Watanabe, Fumiya / Zhou, Guo-Lei / Biris, Alexandru S / Xie, Jennifer Yanhua

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 6

Abstract: Gold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). ...

Abstract	Gold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). AuNRs were synthesized according to the seed-mediated method resulting in nanorods with an aspect ratio of around 3 (~12 nm diameter, 36 nm length) and plasmon resonance at 520 and 780 nm, as confirmed by transmission electron microscopy (TEM) and UV-vis spectroscopy, respectively. A layer-by-layer approach was used to fabricate the AuNR substrate on the functionalized glass coverslips. NSCs were propagated for 10 days using fibroblast growth factor, platelet-derived growth-factor-supplemented culture media, and differentiated on an AuNR or poly-D-lysine (PDL)-coated surface using differentiation media containing triiodothyronine for three weeks. Results showed that NSCs survived better and differentiated faster on the AuNRs compared to the PDL surface. By week 1, almost all cells had differentiated on the AuNR substrate, whereas only ~60% differentiated on the PDL surface, with similar percentages of ODCs and astrocytes. This study indicates that functionalized AuNR substrate does promote NSC differentiation and could be a viable tool for tissue engineering to support the differentiation of stem cells.
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12060929
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Graphene-based 2D constructs for enhanced fibroblast support.

Safina, Ingrid / Bourdo, Shawn E / Algazali, Karrer M / Kannarpady, Ganesh / Watanabe, Fumiya / Vang, Kieng Bao / Biris, Alexandru S

PloS one

2020 Volume 15, Issue 5, Page(s) e0232670

Abstract: Complex skin wounds have always been a significant health and economic problem worldwide due to their elusive and sometimes poor or non-healing conditions. If not well-treated, such wounds may lead to amputation, infections, cancer, or even death. Thus, ... ...

Abstract	Complex skin wounds have always been a significant health and economic problem worldwide due to their elusive and sometimes poor or non-healing conditions. If not well-treated, such wounds may lead to amputation, infections, cancer, or even death. Thus, there is a need to efficiently generate multifunctional skin grafts that address a wide range of skin conditions, including non-healing wounds, and enable the regeneration of new skin tissue. Here, we propose studying pristine graphene and two of its oxygen-functionalized derivatives-high and low-oxygen graphene films-as potential substrates for skin cell proliferation and differentiation. Using BJ cells (human foreskin-derived fibroblasts) to represent basic skin cells, we show that the changes in surface properties of pristine graphene due to oxygen functionalization do not seem to statistically impact the normal proliferation and maturation of skin cells. Our results indicate that the pristine and oxidized graphenes presented relatively low cytotoxicity to BJ fibroblasts and, in fact, support their growth and bioactivity. Therefore, these graphene films could potentially be integrated into more complex skin regenerative systems to support skin regeneration. Because graphene's surface can be relatively easily functionalized with various chemical groups, this finding presents a major opportunity for the development of various composite materials that can act as active components in regenerative applications such as skin regeneration.
MeSH term(s)	Cell Line ; Cell Proliferation ; Cell Survival ; Fibroblasts/cytology ; Graphite/chemistry ; Humans ; Surface Properties ; Tissue Engineering ; Tissue Scaffolds/chemistry
Chemical Substances	graphene oxide ; Graphite (7782-42-5)
Language	English
Publishing date	2020-05-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0232670
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito