Article: Coordinate expression of anticoagulant heparan sulfate proteoglycans and serine protease inhibitors in the rat ovary: a potent system of proteolysis control.
2002 Volume 66, Issue 1, Page(s) 144–158
Abstract: During the reproductive cycle, ovarian follicles undergo major tissue-remodeling involving vascular changes and proteolysis. Anticoagulant heparan sulfate proteoglycans (aHSPGs) are expressed by granulosa cells during the development of the ovarian ... ...
| Abstract | During the reproductive cycle, ovarian follicles undergo major tissue-remodeling involving vascular changes and proteolysis. Anticoagulant heparan sulfate proteoglycans (aHSPGs) are expressed by granulosa cells during the development of the ovarian follicle. The function of aHSPGs in the ovary is unknown, but they might be involved in proteolysis control through binding and activation of serine protease inhibitors. To identify functional interactions between aHSPGs and heparin-binding protease inhibitors in the follicle, we have coordinately localized aHSPGs, antithrombin III, protease nexin-1, and plasminogen activator inhibitor-1 in the rat ovary during natural and gonadotropin-stimulated cycles. Anticoagulant HSPGs were visualized by autoradiography of cryosections incubated with 125I-antithrombin III, and protease inhibitors were assessed by immunohistochemistry and Northern blot hybridization. Anticoagulant HSPGs were expressed in follicles before ovulation, were transiently decreased in postovulatory follicles, and were abundant in the corpus luteum, mainly on capillaries. Anticoagulant HSPGs were colocalized with protease nexin-1 in follicles from the early antral stage until ovulation, with antithrombin III in the preovulatory stage and after ovulation, and with plasminogen activator inhibitor-1 in the corpus luteum. These data demonstrate that aHSPGs are critically expressed in the ovary to interact sequentially with protease nexin-1, antithrombin III, and plasminogen activator inhibitor-1 during the cycle. The specificity of these inhibitors is shifted toward thrombin inhibition in the presence of heparin, suggesting that aHSPGs direct their action to control fibrin deposition in the follicle. The occupation of aHSPGs antithrombin-binding sites by mutant R393C antithrombin III, injected in the ovarian bursa, decreased ovulation efficiency, further supporting the involvement of aHSPGs in the ovulation process. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Amyloid beta-Protein Precursor ; Animals ; Anticoagulants/blood ; Blotting, Northern ; Carrier Proteins/metabolism ; Cell Line ; Cricetinae ; Estrous Cycle/physiology ; Female ; Fibrin/metabolism ; Granulosa Cells/metabolism ; Heparan Sulfate Proteoglycans/biosynthesis ; Heparan Sulfate Proteoglycans/physiology ; Immunohistochemistry ; Ovary/metabolism ; Ovulation/physiology ; Plasminogen Activator Inhibitor 1/biosynthesis ; Protease Nexins ; RNA/isolation & purification ; Rats ; Rats, Sprague-Dawley ; Receptors, Cell Surface ; Serine Proteinase Inhibitors/biosynthesis ; Serpins/biosynthesis | |||||
| Chemical Substances | Amyloid beta-Protein Precursor ; Anticoagulants ; Carrier Proteins ; Heparan Sulfate Proteoglycans ; Plasminogen Activator Inhibitor 1 ; Protease Nexins ; Receptors, Cell Surface ; Serine Proteinase Inhibitors ; Serpins ; RNA (63231-63-0) ; Fibrin (9001-31-4) | |||||
| Language | English | |||||
| Publishing date | 2002-01 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1118-6 | |||||
| ISSN | 1529-7268 ; 0006-3363 | |||||
| ISSN (online) | 1529-7268 | |||||
| ISSN | 0006-3363 | |||||
| DOI | 10.1095/biolreprod66.1.144 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 551: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.