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  1. Article ; Online: Candida auris biofilm: a review on model to mechanism conservation.

    Khari, Arsha / Biswas, Biswambhar / Gangwar, Garima / Thakur, Anil / Puria, Rekha

    Expert review of anti-infective therapy

    2023  Volume 21, Issue 3, Page(s) 295–308

    Abstract: Introduction: Candida auris: Areas covered: This review provides brief insights into the understanding of : Expert opinion: The selection of an appropriate model system is a must for the accuracy and reproducibility of results. The conservation of ...

    Abstract Introduction: Candida auris
    Areas covered: This review provides brief insights into the understanding of
    Expert opinion: The selection of an appropriate model system is a must for the accuracy and reproducibility of results. The conservation of major signaling pathways in
    MeSH term(s) Humans ; Candida/physiology ; Candida auris ; Reproducibility of Results ; Saccharomyces cerevisiae ; Biofilms ; Candida albicans ; Antifungal Agents/pharmacology
    Chemical Substances Antifungal Agents
    Language English
    Publishing date 2023-02-16
    Publishing country England
    Document type Systematic Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2181279-2
    ISSN 1744-8336 ; 1478-7210
    ISSN (online) 1744-8336
    ISSN 1478-7210
    DOI 10.1080/14787210.2023.2179036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Novel Robust Method Mimicking Human Substratum To Dissect the Heterogeneity of Candida auris Biofilm Formation.

    Biswas, Biswambhar / Rana, Aishwarya / Gupta, Nidhi / Gupta, Ishaan / Puria, Rekha / Thakur, Anil

    Microbiology spectrum

    2023  Volume 11, Issue 4, Page(s) e0089223

    Abstract: Candida auris is a pathogen of urgent threat level as marked by the CDC. The formation of biofilms is an essential property of this fungus to establish infection and escape drug treatment. However, our understanding of pathogenesis through biofilm is ... ...

    Abstract Candida auris is a pathogen of urgent threat level as marked by the CDC. The formation of biofilms is an essential property of this fungus to establish infection and escape drug treatment. However, our understanding of pathogenesis through biofilm is hampered by heterogeneity in C. auris biofilms observed in different studies. It is imperative to replicate
    MeSH term(s) Humans ; Antifungal Agents/pharmacology ; Candida auris ; Gelatin/pharmacology ; Candida ; Candida albicans ; Biofilms ; Candida glabrata
    Chemical Substances Antifungal Agents ; Gelatin (9000-70-8)
    Language English
    Publishing date 2023-07-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.00892-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation.

    Pavale, Siddhi / Dalei, Sudipt Kumar / Sokhal, Preeti / Biswas, Biswambhar / Meena, Kunal / Adlakha, Nidhi

    Biotechnology journal

    2023  Volume 19, Issue 1, Page(s) e2300275

    Abstract: Phenylalanine ammonia-lyase (PAL) is a crucial enzyme for various biotechnology applications, such as producing phenols, antioxidants, and nutraceuticals. However, feedback inhibition from its product, cinnamic acid, limits its forward reaction rate. ... ...

    Abstract Phenylalanine ammonia-lyase (PAL) is a crucial enzyme for various biotechnology applications, such as producing phenols, antioxidants, and nutraceuticals. However, feedback inhibition from its product, cinnamic acid, limits its forward reaction rate. Therefore, this study aims to address the feedback inhibition in PAL using enzyme engineering strategies. Random and site-directed mutagenesis approaches were utilized to screen mutant enzymes with ameliorated tolerance against cinnamic acid. A thermotolerant and cinnamate-tolerant mutant was rationally identified using a high throughput screening method and subsequent biochemical characterization. We evaluated cinnamate affinity among the seven rationally selected mutations, and the T102E mutation was identified as the most promising mutant. This mutant showed a six-fold reduction in the affinity of PAL for cinnamic acid and a two-fold increase in operational stability compared with native PAL. Furthermore, the enzyme was immobilized on carbon nanotubes to increase its robustness and reusability. The immobilized mutant PAL showed greater efficiency in the deamination of phenylalanine present in protein hydrolysate than its free form. The rationale behind the enhancement of cinnamate tolerance was validated using molecular dynamic simulations. Overall, the knowledge of the sequence-function relationship of PAL was applied to drive enzyme engineering to develop highly tolerant PAL.
    MeSH term(s) Phenylalanine Ammonia-Lyase/genetics ; Phenylalanine Ammonia-Lyase/chemistry ; Phenylalanine Ammonia-Lyase/metabolism ; Feedback ; Nanotubes, Carbon ; Cinnamates ; Biotransformation
    Chemical Substances Phenylalanine Ammonia-Lyase (EC 4.3.1.24) ; cinnamic acid (140-10-3) ; Nanotubes, Carbon ; Cinnamates
    Language English
    Publishing date 2023-10-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.202300275
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Rapamycin and Torin2 inhibit

    Biswas, Biswambhar / Gangwar, Garima / Nain, Vikrant / Gupta, Ishaan / Thakur, Anil / Puria, Rekha

    Journal of biomolecular structure & dynamics

    2022  Volume 41, Issue 17, Page(s) 8445–8461

    Abstract: ... The ... ...

    Abstract The fungus
    Language English
    Publishing date 2022-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2022.2134927
    Database MEDical Literature Analysis and Retrieval System OnLINE

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