Artikel ; Online: Relaxant effects of sildenafil on the human isolated bladder neck.
2009 Band 73, Heft 2, Seite(n) 427–430
Abstract: Objective: To evaluate potential in vitro relaxant actions of sildenafil on human isolated bladder neck smooth muscle.: Methods: Bladder neck strips were sampled from patients (aged 55-77 years) submitted to prostatic surgery (6 adenomectomies and 1 ... ...
| Abstract | Objective: To evaluate potential in vitro relaxant actions of sildenafil on human isolated bladder neck smooth muscle. Methods: Bladder neck strips were sampled from patients (aged 55-77 years) submitted to prostatic surgery (6 adenomectomies and 1 radical prostatectomy). These were carefully dissected into 1-2 x 0.5-cm pieces and suspended in an organ bath containing 30 mL of a modified Krebs Henseleit solution, bubbled with 95% O(2)/5% CO(2). After tissue stabilization and viability test with KCl, the tissue was precontracted with phenylephrine, and a concentration-response relaxant curve to sildenafil was constructed. The effect of sildenafil was also assessed in tissues treated with N(omega)-nitro-L-arginine methyl ester (L-NAME) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one (ODQ) (inhibitors of nitric oxide synthase and guanylyl cyclase, respectively). Result: Sildenafil induced significant bladder neck relaxation at all concentrations tested. The maximum relaxation was 86.97% +/- 6.69%, obtained with a high concentration of sildenafil (5.1 x 10(-4) M). Both L-NAME and ODQ significantly reduced sildenafil-induced relaxation. Conclusions: Sildenafil was effective in inducing bladder neck smooth muscle relaxation in vitro. This effect was almost abolished by L-NAME and ODQ, clearly demonstrating a dependence of the nitric oxide-cyclic guanosine monophosphate pathway. Our in-vitro results suggest that sildenafil might be useful in improving lower urinary tract symptoms due to benign prostatic hyperplasia. |
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| Mesh-Begriff(e) | Aged ; Humans ; In Vitro Techniques ; Male ; Middle Aged ; Muscle Relaxation/drug effects ; Phosphodiesterase Inhibitors/pharmacology ; Piperazines/pharmacology ; Purines/pharmacology ; Sildenafil Citrate ; Sulfones/pharmacology ; Urinary Bladder/drug effects ; Urinary Bladder/physiology | ||||||||||
| Chemische Substanzen | Phosphodiesterase Inhibitors ; Piperazines ; Purines ; Sulfones ; Sildenafil Citrate (BW9B0ZE037) | ||||||||||
| Sprache | Englisch | ||||||||||
| Erscheinungsdatum | 2009-02 | ||||||||||
| Erscheinungsland | United States | ||||||||||
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't | ||||||||||
| ZDB-ID | 192062-5 | ||||||||||
| ISSN | 1527-9995 ; 0090-4295 | ||||||||||
| ISSN (online) | 1527-9995 | ||||||||||
| ISSN | 0090-4295 | ||||||||||
| DOI | 10.1016/j.urology.2008.06.060 | ||||||||||
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| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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