LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 3 of total 3

Search options

  1. Article: Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform.

    Kimpston-Burkgren, Kay / Mora-Díaz, Juan Carlos / Roby, Philippe / Bjustrom-Kraft, Jordan / Main, Rodger / Bosse, Roger / Giménez-Lirola, Luis Gabriel

    Pathogens (Basel, Switzerland)

    2020  Volume 9, Issue 3

    Abstract: Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging ... ...

    Abstract Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic
    Keywords covid19
    Language English
    Publishing date 2020-03-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens9030233
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

    Kay, Kimpston-Burkgren / Mora-Díaz, Juan Carlos / Roby, Philippe / Bjustrom-Kraft, Jordan / Main, Rodger / Bosse, Roger / Giménez-Lirola, Luis Gabriel

    Pathogens

    Abstract: Coronavirus infections are a continuous threat raised time and again With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health Porcine epidemic diarrhea (PED) is considered to be a reemerging ... ...

    Abstract Coronavirus infections are a continuous threat raised time and again With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV) In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA) PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection PEDV IgG antibodies were detected for at least 16 weeks post-exposure Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the “time to result” metric of currently available immunoassays
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #826966
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs.

    Bjustrom-Kraft, Jordan / Woodard, Katie / Giménez-Lirola, Luis / Rotolo, Marisa / Wang, Chong / Sun, Yaxuan / Lasley, Peter / Zhang, Jianqiang / Baum, David / Gauger, Phillip / Main, Rodger / Zimmerman, Jeffrey

    BMC veterinary research

    2016  Volume 12, Page(s) 99

    Abstract: Background: Longitudinal samples from two production sites were used to (1) describe the pattern of PEDV shedding (rRT-PCR) in individual rectal swabs, pen fecal samples, and pen oral fluids (OF); (2) describe the kinetics of PEDV antibody by ELISA (IgA, ...

    Abstract Background: Longitudinal samples from two production sites were used to (1) describe the pattern of PEDV shedding (rRT-PCR) in individual rectal swabs, pen fecal samples, and pen oral fluids (OF); (2) describe the kinetics of PEDV antibody by ELISA (IgA, IgG) testing of pig serum and pen oral fluid samples; and (3) establish cutoffs and performance estimates for PEDV WV ELISAs (IgA, IgG). Site One was PEDV positive; Site Two was PEDV negative. On Site One, pen samples (feces and oral fluids) and pig samples (rectal swabs and sera) were collected both before and after the population was exposed to PEDV.
    Results: On Site Two, pen oral fluid samples and individual pig serum samples were negative for both PEDV antibody and nucleic acid. On Site One, PEDV was detected by rRT-PCR at 6 days post exposure (DPE) in all sample types. The last rRT-PCR positives were detected in rectal swabs and oral fluids on 69 DPE. IgG and IgA were detected in oral fluids and serum samples by 13 DPE. Analysis of the PEDV serum IgG WV ELISA data showed that a sample-to-positive (S/P) cutoff of ≥ 0.80 provided a diagnostic sensitivity of 0.87 (95% CI: 0.82, 0.91) and specificity of 0.99 (95% CI: 0.98, 1.00). Serum IgG results declined slowly over the monitoring period, with 60% of serum samples positive (S/P ≥ 0.80) at the final sampling on 111 DPE. Analysis of the PEDV oral fluid IgA WV ELISA found that a cutoff of S/P ≥ 0.80 provided a diagnostic sensitivity of 1.00 (95% CI: 0.92, 1.00) and a diagnostic specificity of 1.00 (95% CI: 0.99, 1.00). The oral fluid IgA response increased through 96 DPE and began to decline at the last sampling on 111 DPE.
    Conclusions: This study showed that oral fluid-based testing could provide an easy and "animal-friendly" approach to sample collection for nucleic acid and/or antibody-based surveillance of PEDV in swine populations.
    MeSH term(s) Animals ; Antibodies, Viral/analysis ; Coronavirus Infections/diagnosis ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Enzyme-Linked Immunosorbent Assay/veterinary ; Feces/virology ; Porcine epidemic diarrhea virus/isolation & purification ; Real-Time Polymerase Chain Reaction/veterinary ; Rectum/virology ; Saliva/virology ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/virology ; Virus Shedding
    Chemical Substances Antibodies, Viral
    Keywords covid19
    Language English
    Publishing date 2016-06-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1746-6148
    ISSN (online) 1746-6148
    DOI 10.1186/s12917-016-0725-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top