Article ; Online: High-Throughput Production of Proteins in E. coli for Structural Studies.
Methods in molecular biology (Clifton, N.J.)
2017 Volume 1586, Page(s) 359–371
Abstract: We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target proteins. It includes a one-step purification for ... ...
| Abstract | We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target proteins. It includes a one-step purification for the purposes of functional assays and a two-step protocol for crystallographic studies, with the option of on-column tag removal. |
|---|---|
| Language | English |
| Publishing date | 2017 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-4939-6887-9_24 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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