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  1. Article ; Online: Real-Time and High-Resolution Monitoring of Neuronal Electrical Activity and pH Variations Based on the Co-Integration of Nanoelectrodes and Chem-FinFETs.

    Bettamin, Luca / Mathieu, Fabrice / Marty, Florent H / Blatche, Marie Charline / Gonzalez-Dunia, Daniel / Suberbielle, Elsa / Larrieu, Guilhem

    Small (Weinheim an der Bergstrasse, Germany)

    2024  , Page(s) e2309055

    Abstract: Developing new approaches amenable to the measurement of neuronal physiology in real-time is a very active field of investigation, as it will offer improved methods to assess the impact of diverse insults on neuronal homeostasis. Here, the development of ...

    Abstract Developing new approaches amenable to the measurement of neuronal physiology in real-time is a very active field of investigation, as it will offer improved methods to assess the impact of diverse insults on neuronal homeostasis. Here, the development of an in vitro bio platform is reported which can record the electrical activity of cultured primary rat cortical neurons with extreme sensitivity, while simultaneously tracking the localized changes in the pH of the culture medium. This bio platform features passive vertical nanoprobes with ultra-high signal resolution (several mV amplitude ranges) and Chem-FinFETs (pH sensitivity of sub-0.1 pH units), covering an area as little as a neuronal soma. These multi-sensing units are arranged in an array to probe both chemically and electrically an equivalent surface of ≈ 0.5 mm
    Language English
    Publishing date 2024-03-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2168935-0
    ISSN 1613-6829 ; 1613-6810
    ISSN (online) 1613-6829
    ISSN 1613-6810
    DOI 10.1002/smll.202309055
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  2. Article: Carbon nanofiber-PEDOT composite films as novel microelectrode for neural interfaces and biosensing

    Saunier, Valentin / Flahaut, Emmanuel / Blatché, Marie-Charline / Bergaud, Christian / Maziz, Ali

    Biosensors & bioelectronics. 2020 Oct. 01, v. 165

    2020  

    Abstract: A clear need exists for novel nanostructured materials that are capable to meet the performance criteria of a number of neuronal therapies including neural recording, stimulation and sensing of bioactive molecules at the electrode-tissue interface. By ... ...

    Abstract A clear need exists for novel nanostructured materials that are capable to meet the performance criteria of a number of neuronal therapies including neural recording, stimulation and sensing of bioactive molecules at the electrode-tissue interface. By combining Poly (3,4-ethylenedioxythiophene) (PEDOT), with Carbon Nanofibers (CNFs), we demonstrate a versatile approach for the synthesis of a novel composite material PEDOT:CNF with remarkable electrochemical properties, combining low impedance, high surface area, high charge injection capability and reliable neurotransmitters monitoring using amperometric techniques. The oxidized CNFs were utilized as dopants of PEDOT to prepare the composite coatings through electrochemical deposition on neural microelectrodes arrays (MEA). The PEDOT:CNF modified microelectrodes demonstrated the low specific impedance of 1.28 MΩ μm² at 1 kHz and results in unrivalled charge injection limit of 10.03 mC/cm² when compared to other reported organic electrode nanomaterials. Furthermore, amperometric detection performances were determined for the neurotransmitters dopamine and serotonin, exhibiting linear concentration range from 0.1 to 9 μM and from 0.06 to 9 μM respectively, high sensitivities (44.54 pA/nM.μm² and 71.08 pA/nM.μm², respectively) and low detection limits (0.045 μM and 0.056 μM, respectively). Cell viability was investigated on PEDOT:CNF coated microelectrodes to show that the composite material does not advocate any cytotoxicity. Taken together, these results suggest the great potential of PEDOT:CNF composite for developing next-generation multifunctional microelectrodes for applications in neural therapies.
    Keywords bioactive compounds ; biosensors ; carbon ; carbon nanofibers ; cell viability ; coatings ; composite films ; composite materials ; cytotoxicity ; detection limit ; dopamine ; electrochemistry ; microelectrodes ; monitoring ; neurons ; neurotransmitters ; oxidation ; serotonin ; surface area
    Language English
    Dates of publication 2020-1001
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2020.112413
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Microelectrodes from PEDOT-carbon nanofiber composite for high performance neural recording, stimulation and neurochemical sensing.

    Saunier, Valentin / Flahaut, Emmanuel / Blatché, Marie-Charline / Bergaud, Christian / Maziz, Ali

    MethodsX

    2020  Volume 7, Page(s) 101106

    Abstract: This present method describes a versatile approach for the electrochemical synthesis of a composite material of Poly (3,4-ethylenedioxythiophene) (PEDOT) and Carbon Nanofibers (CNFs) for neural interfaces and biosensing applications. Oxidized CNFs were ... ...

    Abstract This present method describes a versatile approach for the electrochemical synthesis of a composite material of Poly (3,4-ethylenedioxythiophene) (PEDOT) and Carbon Nanofibers (CNFs) for neural interfaces and biosensing applications. Oxidized CNFs were utilized as dopants of PEDOT to prepare the composite coating through electrochemical deposition on microelectrodes arrays (MEA). The experimental results of this study showed that PEDOT:CNF microelectrodes exhibit remarkable electrochemical properties, combining low impedance, high surface area, high charge injection capability and reliable neurotransmitters monitoring using amperometric techniques. Taken together, these results suggest the great potential of PEDOT:CNF composite for developing next-generation multifunctional microelectrodes for applications in neural therapies.•A simple approach for the electrochemical synthesis of PEDOT:CNF composite material on microelectrodes for neural interfaces and neurochemical sensing.•PEDOT:CNF microelectrodes exhibit remarkable electrochemical properties, combining low impedance and high charge injection capabilities.•PEDOT:CNF microelectrodes allowed the reliable detection of neurotransmitters with improved sensitivity.
    Language English
    Publishing date 2020-10-17
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2020.101106
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  4. Article ; Online: Carbon nanofiber-PEDOT composite films as novel microelectrode for neural interfaces and biosensing.

    Saunier, Valentin / Flahaut, Emmanuel / Blatché, Marie-Charline / Bergaud, Christian / Maziz, Ali

    Biosensors & bioelectronics

    2020  Volume 165, Page(s) 112413

    Abstract: A clear need exists for novel nanostructured materials that are capable to meet the performance criteria of a number of neuronal therapies including neural recording, stimulation and sensing of bioactive molecules at the electrode-tissue interface. By ... ...

    Abstract A clear need exists for novel nanostructured materials that are capable to meet the performance criteria of a number of neuronal therapies including neural recording, stimulation and sensing of bioactive molecules at the electrode-tissue interface. By combining Poly (3,4-ethylenedioxythiophene) (PEDOT), with Carbon Nanofibers (CNFs), we demonstrate a versatile approach for the synthesis of a novel composite material PEDOT:CNF with remarkable electrochemical properties, combining low impedance, high surface area, high charge injection capability and reliable neurotransmitters monitoring using amperometric techniques. The oxidized CNFs were utilized as dopants of PEDOT to prepare the composite coatings through electrochemical deposition on neural microelectrodes arrays (MEA). The PEDOT:CNF modified microelectrodes demonstrated the low specific impedance of 1.28 MΩ μm
    MeSH term(s) Biosensing Techniques ; Bridged Bicyclo Compounds, Heterocyclic ; Carbon ; Microelectrodes ; Nanofibers ; Polymers
    Chemical Substances Bridged Bicyclo Compounds, Heterocyclic ; Polymers ; poly(3,4-ethylene dioxythiophene) ; Carbon (7440-44-0)
    Language English
    Publishing date 2020-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2020.112413
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Self-Aligned Functionalization Approach to Order Neuronal Networks at the Single-Cell Level.

    Casanova, Adrien / Blatche, Marie-Charline / Ferre, Cécile A / Martin, Hélène / Gonzalez-Dunia, Daniel / Nicu, Liviu / Larrieu, Guilhem

    Langmuir : the ACS journal of surfaces and colloids

    2018  Volume 34, Issue 22, Page(s) 6612–6620

    Abstract: Despite significant progress, our knowledge of the functioning of the central nervous system still remains scarce to date. A better understanding of its behavior, in either normal or diseased conditions, goes through an increased knowledge of basic ... ...

    Abstract Despite significant progress, our knowledge of the functioning of the central nervous system still remains scarce to date. A better understanding of its behavior, in either normal or diseased conditions, goes through an increased knowledge of basic mechanisms involved in neuronal function, including at the single-cell level. This has motivated significant efforts for the development of miniaturized sensing devices to monitor neuronal activity with high spatial and signal resolution. One of the main challenges remaining to be addressed in this domain is, however, the ability to create in vitro spatially ordered neuronal networks at low density with a precise control of the cell location to ensure proper monitoring of the activity of a defined set of neurons. Here, we present a novel self-aligned chemical functionalization method, based on a repellant surface with patterned attractive areas, which permits the elaboration of low-density neuronal network down to individual cells with a high control of the soma location and axonal growth. This approach is compatible with complementary metal-oxide-semiconductor line technology at a wafer scale and allows performing the cell culture on packaged chip outside microelectronics facilities. Rat cortical neurons were cultured on such patterned surfaces for over one month and displayed a very high degree of organization in large networks. Indeed, more than 90% of the network nodes were settled by a soma and 100% of the connecting lines were occupied by a neurite, with a very good selectivity (low parasitic cell connections). After optimization, networks composed of 75% of unicellular nodes were obtained, together with a control at the micron scale of the location of the somas. Finally, we demonstrated that the dendritic neuronal growth was guided by the surface functionalization, even when micrometer scale topologies were encountered and we succeeded to control the extension growth along one-dimensional-aligned nanostructures with sub-micrometrical scale precision. This novel approach now opens the way for precise monitoring of neuronal network activity at the single-cell level.
    MeSH term(s) Animals ; Cell Culture Techniques/methods ; Cells, Cultured ; Dendrites ; Nerve Net/chemistry ; Nerve Net/metabolism ; Neurites ; Neurons/cytology ; Rats
    Language English
    Publishing date 2018-05-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/acs.langmuir.8b00529
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  6. Article ; Online: Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization.

    Accardo, Angelo / Blatché, Marie-Charline / Courson, Rémi / Loubinoux, Isabelle / Thibault, Christophe / Malaquin, Laurent / Vieu, Christophe

    Small (Weinheim an der Bergstrasse, Germany)

    2017  Volume 13, Issue 27

    Abstract: The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. ...

    Abstract The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology. Scanning electron microscopy (SEM) provides detailed insights about the morphology of both cells and cellular interconnections around the 3D architecture. On the other hand, the nature of the seeding in the inner core of the 3D scaffold, inaccessible by conventional SEM imaging, is unveiled by light sheet fluorescence microscopy and multiphoton confocal imaging highlighting an optimal cell colonization both around and within the 3D scaffold as well as the formation of long neuritic extensions. The results open appealing scenarios for the use of the developed 3D fabrication/3D imaging protocols in several neuroscientific contexts.
    MeSH term(s) Biocompatible Materials/chemistry ; Cell Line, Tumor ; Humans ; Imaging, Three-Dimensional/methods ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Polymers/chemistry ; Tissue Engineering/methods ; Tissue Scaffolds/chemistry
    Chemical Substances Biocompatible Materials ; Polymers
    Language English
    Publishing date 2017-05-30
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1613-6829
    ISSN (online) 1613-6829
    DOI 10.1002/smll.201700621
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