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  1. Article ; Online: Differentially methylated genes involved in reproduction and ploidy levels in recent diploidized and tetraploidized Eragrostis curvula genotypes.

    Carballo, J / Achilli, A / Hernández, F / Bocchini, M / Pasten, M C / Marconi, G / Albertini, E / Zappacosta, D / Echenique, V

    Plant reproduction

    2023  

    Abstract: Epigenetics studies changes in gene activity without changes in the DNA sequence. Methylation is an epigenetic mechanism important in many pathways, such as biotic and abiotic stresses, cell division, and reproduction. Eragrostis curvula is a grass ... ...

    Abstract Epigenetics studies changes in gene activity without changes in the DNA sequence. Methylation is an epigenetic mechanism important in many pathways, such as biotic and abiotic stresses, cell division, and reproduction. Eragrostis curvula is a grass species reproducing by apomixis, a clonal reproduction by seeds. This work employed the MCSeEd technique to identify deferentially methylated positions, regions, and genes in the CG, CHG, and CHH contexts in E. curvula genotypes with similar genomic backgrounds but with different reproductive modes and ploidy levels. In this way, we focused the analysis on the cvs. Tanganyika INTA (4x, apomictic), Victoria (2x, sexual), and Bahiense (4x, apomictic). Victoria was obtained from the diploidization of Tanganyika INTA, while Bahiense was produced from the tetraploidization of Victoria. This study showed that polyploid/apomictic genotypes had more differentially methylated positions and regions than the diploid sexual ones. Interestingly, it was possible to observe fewer differentially methylated positions and regions in CG than in the other contexts, meaning CG methylation is conserved across the genotypes regardless of the ploidy level and reproductive mode. In the comparisons between sexual and apomictic genotypes, we identified differentially methylated genes involved in the reproductive pathways, specifically in meiosis, cell division, and fertilization. Another interesting observation was that several differentially methylated genes between the diploid and the original tetraploid genotype recovered their methylation status after tetraploidization, suggesting that methylation is an important mechanism involved in reproduction and ploidy changes.
    Language English
    Publishing date 2023-12-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2700598-7
    ISSN 2194-7961 ; 2194-7953
    ISSN (online) 2194-7961
    ISSN 2194-7953
    DOI 10.1007/s00497-023-00490-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Delta-like ligand 3 (DLL3): an attractive actionable target in tumors with neuroendocrine origin.

    Ranallo, Nicoletta / Bocchini, Martine / Menis, Jessica / Pilotto, Sara / Severi, Stefano / Liverani, Chiara / Bongiovanni, Alberto

    Expert review of anticancer therapy

    2022  Volume 22, Issue 6, Page(s) 597–603

    Abstract: Introduction: Neuroendocrine carcinomas are very aggressive tumors with few treatment options. DLL3 seems to be an optimal target for therapeutic intervention, as it is expressed mainly on the membrane of tumor cells with neuroendocrine origin.: Areas ...

    Abstract Introduction: Neuroendocrine carcinomas are very aggressive tumors with few treatment options. DLL3 seems to be an optimal target for therapeutic intervention, as it is expressed mainly on the membrane of tumor cells with neuroendocrine origin.
    Areas covered: In this article, we outline the preclinical and clinical studies published in the last years on DLL3 in neuroendocrine neoplasm, above all of lung origin. Furthermore, we review the current literature on the interaction between DLL3 and the tumor microenvironment.
    Expert opinion: Several DLL3-targeting strategies have been proposed in the last years with mixed results. Understanding the influence of DLL3 on the tumor (immune) microenvironment and developing adoptive therapies directed against this optimal target might represent the key strategy. Building on the clinical data obtained so far, future trials on in vivo diagnostic tools for predictive purpose and new specific therapies are needed.
    MeSH term(s) Humans ; Intracellular Signaling Peptides and Proteins/therapeutic use ; Ligands ; Lung Neoplasms/drug therapy ; Membrane Proteins ; Small Cell Lung Carcinoma/pathology ; Tumor Microenvironment
    Chemical Substances DLL3 protein, human ; Intracellular Signaling Peptides and Proteins ; Ligands ; Membrane Proteins
    Language English
    Publishing date 2022-05-04
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2112544-2
    ISSN 1744-8328 ; 1473-7140
    ISSN (online) 1744-8328
    ISSN 1473-7140
    DOI 10.1080/14737140.2022.2071703
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5907: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Environmental and Genetic Factors Affecting Apospory Expressivity in Diploid

    Soliman, Mariano / Bocchini, Marika / Stein, Juliana / Ortiz, Juan Pablo A / Albertini, Emidio / Delgado, Luciana

    Plants (Basel, Switzerland)

    2021  Volume 10, Issue 10

    Abstract: In angiosperms, gametophytic apomixis (clonal reproduction through seeds) is strongly associated with polyploidy and hybridization. The trait is facultative and its expressivity is highly variable between genotypes. Here, we used an ... ...

    Abstract In angiosperms, gametophytic apomixis (clonal reproduction through seeds) is strongly associated with polyploidy and hybridization. The trait is facultative and its expressivity is highly variable between genotypes. Here, we used an F
    Language English
    Publishing date 2021-10-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants10102100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Malignant Pleural Mesothelioma: State-of-the-Art on Current Therapies and Promises for the Future.

    Nicolini, Fabio / Bocchini, Martine / Bronte, Giuseppe / Delmonte, Angelo / Guidoboni, Massimo / Crinò, Lucio / Mazza, Massimiliano

    Frontiers in oncology

    2020  Volume 9, Page(s) 1519

    Abstract: Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer of the pleural surface associated with asbestos exposure. The median survival of MPM patients is a mere 8-14 months, and there are few biomarkers and no cure available. It is hoped that, ... ...

    Abstract Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer of the pleural surface associated with asbestos exposure. The median survival of MPM patients is a mere 8-14 months, and there are few biomarkers and no cure available. It is hoped that, eventually, the incidence of MPM will drop and remain low and constant, given that most nations have banned the use of asbestos, but in the meantime, the incidence in Europe is still growing. The exact molecular mechanisms that explain the carcinogenicity of asbestos are not known. Standard therapeutic strategies for MPM include surgery, often coupled with chemotherapy and/or radiotherapy, in a small percentage of eligible patients and chemotherapy in tumors considered unresectable with or without adjuvant radiotherapy. In recent years, several new therapeutic avenues are being explored. These include angiogenesis inhibitors, synthetic lethal treatment, miRNA replacement, oncoviral therapies, and the fast-growing field of immunotherapy alone or in combination with chemotherapy. Of particular promise are the multiple options offered by immunotherapy: immune checkpoint inhibitors, tumor vaccines, and therapies taking advantage of tumor-specific antigens, such as specific therapeutic antibodies or advanced cell-based therapies exemplified by the CAR-T cells. This review comprehensively presents both old and new therapeutic options in MPM, focusing on the results of the numerous recent and on-going clinical trials in the field, including the latest data presented at international meetings (AACR, ASCO, and ESMO) this year, and concludes that more work has to be done in the framework of tailored therapies to identify reliable targets and novel biomarkers to impact MPM management.
    Language English
    Publishing date 2020-01-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2019.01519
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Fully Human Antibodies for Malignant Pleural Mesothelioma Targeting.

    Nicolini, Fabio / Bocchini, Martine / Angeli, Davide / Bronte, Giuseppe / Delmonte, Angelo / Crinò, Lucio / Mazza, Massimiliano

    Cancers

    2020  Volume 12, Issue 4

    Abstract: Immunotherapy is the most promising therapeutic approach against malignant pleural mesothelioma (MPM). Despite technological progress, the number of targetable antigens or specific antibodies is limited, thus hindering the full potential of recent ... ...

    Abstract Immunotherapy is the most promising therapeutic approach against malignant pleural mesothelioma (MPM). Despite technological progress, the number of targetable antigens or specific antibodies is limited, thus hindering the full potential of recent therapeutic interventions. All possibilities of finding new targeting molecules must be exploited. The specificity of targeting is guaranteed by the use of monoclonal antibodies, while fully human antibodies are preferred, as they are functional and generate no neutralizing antibodies. The aim of this review is to appraise the latest advances in screening methods dedicated to the identification and harnessing of fully human antibodies. The scope of identifying useful molecules proceeds along two avenues, i.e., through the antigen-first or binding-first approaches. The first relies on screening human antibody libraries or plasma from immunized transgenic mice or humans to isolate binders to specific antigens. The latter takes advantage of specific binding to tumor cells of antibodies present in phage display libraries or in responders' plasma samples without prior knowledge of the antigens. Additionally, next-generation sequencing analysis of B-cell receptor repertoire pre- and post-therapy in memory B-cells from responders allows for the identification of clones expanded and matured upon treatment. Human antibodies identified can be subsequently reformatted to generate a plethora of therapeutics like antibody-drug conjugates, immunotoxins, and advanced cell-therapeutics such as chimeric antigen receptor-transduced T-cells.
    Keywords covid19
    Language English
    Publishing date 2020-04-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers12040915
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  6. Article: Circulating hsa-miR-5096 predicts

    Bocchini, Martine / Tazzari, Marcella / Ravaioli, Sara / Piccinini, Filippo / Foca, Flavia / Tebaldi, Michela / Nicolini, Fabio / Grassi, Ilaria / Severi, Stefano / Calogero, Raffaele Adolfo / Arigoni, Maddalena / Schrader, Joerg / Mazza, Massimiliano / Paganelli, Giovanni

    Frontiers in oncology

    2023  Volume 13, Page(s) 1136331

    Abstract: Gastro-entero-pancreatic neuroendocrine tumors (GEP-NETs) are rare diseases encompassing pancreatic (PanNETs) and ileal NETs (SINETs), characterized by heterogeneous somatostatin receptors (SSTRs) expression. Treatments for inoperable GEP-NETs are ... ...

    Abstract Gastro-entero-pancreatic neuroendocrine tumors (GEP-NETs) are rare diseases encompassing pancreatic (PanNETs) and ileal NETs (SINETs), characterized by heterogeneous somatostatin receptors (SSTRs) expression. Treatments for inoperable GEP-NETs are limited, and SSTR-targeted Peptide Receptor Radionuclide Therapy (PRRT) achieves variable responses. Prognostic biomarkers for the management of GEP-NET patients are required.
    Methods: Whole miRNOme NGS profiling was conducted on plasma samples obtained from well-differentiated advanced, metastatic, inoperable G1, G2 and G3 GEP-NET patients enrolled in the non-randomized LUX (NCT02736500) and LUNET (NCT02489604) clinical trials prior to PRRT (screening set, n= 24). Differential expression analysis was performed between
    Results: While no miRNAs emerged to be deregulated in SINETs, hsa-miR-5096, hsa-let-7i-3p and hsa-miR-4311 were found to correlate with
    Conclusions: hsa-miR-5096 well performs as a biomarker for
    Language English
    Publishing date 2023-05-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1136331
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  7. Article ; Online: Combining preclinical tools and models to unravel tumor complexity: Jump into the next dimension.

    Miserocchi, Giacomo / Bocchini, Martine / Cortesi, Michela / Arienti, Chiara / De Vita, Alessandro / Liverani, Chiara / Mercatali, Laura / Bravaccini, Sara / Ulivi, Paola / Zanoni, Michele

    Frontiers in immunology

    2023  Volume 14, Page(s) 1171141

    Abstract: Tumors are complex and heterogeneous diseases characterized by an intricate milieu and dynamically in connection with surrounding and distant tissues. In the last decades, great efforts have been made to develop novel preclinical models able to ... ...

    Abstract Tumors are complex and heterogeneous diseases characterized by an intricate milieu and dynamically in connection with surrounding and distant tissues. In the last decades, great efforts have been made to develop novel preclinical models able to recapitulate the original features of tumors. However, the development of an
    MeSH term(s) Animals ; Ecosystem ; Zebrafish ; Neoplasms/pathology ; Organoids ; Spheroids, Cellular/pathology ; Tumor Microenvironment
    Language English
    Publishing date 2023-03-24
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1171141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH.

    Gallerani, Giulia / Cocchi, Claudia / Bocchini, Martine / Piccinini, Filippo / Fabbri, Francesco

    Journal of visualized experiments : JoVE

    2017  , Issue 130

    Abstract: Circulating tumor cells (CTCs) are associated with poor survival in metastatic cancer. Their identification, phenotyping, and genotyping could lead to a better understanding of tumor heterogeneity and thus facilitate the selection of patients for ... ...

    Abstract Circulating tumor cells (CTCs) are associated with poor survival in metastatic cancer. Their identification, phenotyping, and genotyping could lead to a better understanding of tumor heterogeneity and thus facilitate the selection of patients for personalized treatment. However, this is hampered because of the rarity of CTCs. We present an innovative approach for sampling a high volume of the patient blood and obtaining information about presence, phenotype, and gene translocation of CTCs. The method combines immunofluorescence staining and DNA fluorescent-in-situ-hybridization (DNA FISH) and is based on a functionalized medical wire. This wire is an innovative device that permits the in vivo isolation of CTCs from a large volume of peripheral blood. The blood volume screened by a 30-min administration of the wire is approximately 1.5-3 L. To demonstrate the feasibility of this approach, epithelial cell adhesion molecule (EpCAM) expression and the chromosomal translocation of the ALK gene were determined in non-small-cell lung cancer (NSCLC) cell lines captured by the functionalized wire and stained with an immuno-DNA FISH approach. Our main challenge was to perform the assay on a 3D structure, the functionalized wire, and to determine immuno-phenotype and FISH signals on this support using a conventional fluorescence microscope. The results obtained indicate that catching CTCs and analyzing their phenotype and chromosomal rearrangement could potentially represent a new companion diagnostic approach and provide an innovative strategy for improving personalized cancer treatments.
    MeSH term(s) Cell Line, Tumor ; DNA/analysis ; Fluorescent Antibody Technique/methods ; Humans ; In Situ Hybridization, Fluorescence/methods ; Neoplasms/blood ; Neoplasms/pathology ; Neoplastic Cells, Circulating/pathology
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2017--21
    Publishing country United States
    Document type Journal Article ; Video-Audio Media
    ISSN 1940-087X
    ISSN (online) 1940-087X
    DOI 10.3791/56936
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Biomarkers for Pancreatic Neuroendocrine Neoplasms (PanNENs) Management-An Updated Review.

    Bocchini, Martine / Nicolini, Fabio / Severi, Stefano / Bongiovanni, Alberto / Ibrahim, Toni / Simonetti, Giorgia / Grassi, Ilaria / Mazza, Massimiliano

    Frontiers in oncology

    2020  Volume 10, Page(s) 831

    Abstract: Pancreatic neuroendocrine tumors (PanNENs) are rare sporadic cancers or develop as part of hereditary syndromes. PanNENs can be both functioning and non-functioning based on whether they produce bioactive peptides. Some PanNENs are well differentiated ... ...

    Abstract Pancreatic neuroendocrine tumors (PanNENs) are rare sporadic cancers or develop as part of hereditary syndromes. PanNENs can be both functioning and non-functioning based on whether they produce bioactive peptides. Some PanNENs are well differentiated while others-poorly. Symptoms, thus, depend on both oncological and hormonal causes. PanNEN diagnosis and treatment benefit from and in some instances are guided by biomarker monitoring. However, plasmatic monoanalytes are only suggestive of PanNEN pathological status and their positivity is typically followed by deepen diagnostic analyses through imaging techniques. There is a strong need for new biomarkers and follow-up modalities aimed to improve the outcome of PanNEN patients. Liquid biopsy follow-up, i.e., sequential analysis on tumor biomarkers in body fluids offers a great potential, that need to be substantiated by additional studies focusing on the specific markers and the timing of the analyses. This review provides the most updated panorama on PanNEN biomarkers.
    Language English
    Publishing date 2020-05-27
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2020.00831
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  10. Article: Activity and Safety of Immune Checkpoint Inhibitors in Neuroendocrine Neoplasms: A Systematic Review and Meta-Analysis.

    Bongiovanni, Alberto / Maiorano, Brigida Anna / Azzali, Irene / Liverani, Chiara / Bocchini, Martine / Fausti, Valentina / Di Menna, Giandomenico / Grassi, Ilaria / Sansovini, Maddalena / Riva, Nada / Ibrahim, Toni

    Pharmaceuticals (Basel, Switzerland)

    2021  Volume 14, Issue 5

    Abstract: Immune-checkpoint inhibitors (ICIs) have widened the therapeutic scenario of different cancer types. Phase I/II trials have been designed to evaluate the role of ICIs both as single agents and in combination in neuroendocrine neoplasms (NENs), but as yet ...

    Abstract Immune-checkpoint inhibitors (ICIs) have widened the therapeutic scenario of different cancer types. Phase I/II trials have been designed to evaluate the role of ICIs both as single agents and in combination in neuroendocrine neoplasms (NENs), but as yet no randomized controlled phase III trials have been carried out. A systematic review and meta-analysis of studies published could help to reduce the biases of single-phase II trials. Efficacy data were obtained on 636 patients. Pooled percentages of the overall response rate (ORR) and disease control rate (DCR) were 10% (95% CI: 6-15%, I
    Language English
    Publishing date 2021-05-17
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph14050476
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