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Article: New species of Epitoniidae (Gastropoda: Epitonioidea) from the Red Sea

Bonfitto, Antonio

Molluscan research. 2018 Apr. 3, v. 38, no. 2

2018

Abstract: Based on material stored in the Zoological Museum of the University of Bologna (MZB), four new species of epitoniids from the shallow waters of the Red Sea are described: Epitonium (Laeviscala) yemenita n. sp., Epitonium (Laeviscala) morassii n. sp., ... ...

Abstract	Based on material stored in the Zoological Museum of the University of Bologna (MZB), four new species of epitoniids from the shallow waters of the Red Sea are described: Epitonium (Laeviscala) yemenita n. sp., Epitonium (Laeviscala) morassii n. sp., Epitonium (Labeoscala) zabargadense n. sp. and Epitonium (Labeoscala) aranea n. sp. Scanning electron microscope illustrations of type specimens of Scalaria gracilis Sowerby, 1844, Scalaria fucata Pease, 1861, Graciliscala histricosa Jousseaume, 1912, Graciliscala rostrata Jousseaume, 1912, Scala rissoinaeformis Melvill, 1903 and Scala thelcteria Melvill & Standen, 1903 are given. Systematic remarks on some species belonging to the subgenus Laeviscala are treated in additional notes. A lectotype of Graciliscala histricosa is designated. http://zoobank.org/urn:lsid:zoobank.org:pub:8304A24D-5985-43D5-AEBF-1A7ED39A1F1C
Keywords	Gastropoda ; lectotypes ; new species ; scanning electron microscopes ; Red Sea
Language	English
Dates of publication	2018-0403
Size	p. 119-129.
Publishing place	Taylor & Francis
Document type	Article
ISSN	1448-6067
DOI	10.1080/13235818.2017.1385168
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Electron microscopic analysis in the gecko Lygodactylus reveals variations in micro-ornamentation and sensory organs distribution in the epidermis that indicate regional functions.

Bonfitto, Antonio / Bussinello, Davide / Alibardi, Lorenzo

Anatomical record (Hoboken, N.J. : 2007)

2022 Volume 306, Issue 8, Page(s) 1990–2014

Abstract: Possible pattern variations of micro-ornamentation in different areas of the skin in the gecko Lygodactylus have been analyzed by scanning and transmission electron microscopy. A map of micro-ornamentation present in various areas of the skin has been ... ...

Abstract	Possible pattern variations of micro-ornamentation in different areas of the skin in the gecko Lygodactylus have been analyzed by scanning and transmission electron microscopy. A map of micro-ornamentation present in various areas of the skin has been obtained. Differences in micro-ornamentation pattern and sensory organ distribution were detected. The "spinulated pattern" consists of shorter spinulae in dorsal versus ventral scales, and spinules are shorter in inner scale surface and hinge regions with respect to the outer scale surface. The spines derive from the accumulation of struts of corneous material mainly composed of corneous beta proteins (CBPs, formerly indicated as beta-keratins) that merge into pointed micro-ornamentation. The 3D-accumulation of CBPs within Oberhautchen cells can vary in some regions of different scales during Oberhautchen-beta cell differentiation, perhaps also under physical tensile forces derived from continuous scale growth. Three other main patterns of micro-ornamentation were detected and indicated as "corneous belts," "corneous dendritic ramification," and "serpentine-pit and groove." These variations from the typical spinulated pattern present in gecko epidermis are interpreted as transitional regions where the accumulation of corneous material in Oberhautchen cells that merges with underlying beta-cells gives rise to nonspinulated surfaces. Spinulated sensory organs with bristles and lenticular-shaped or knob-like tactile corpuscles are more numerous in ventral scales of the tail tip close to adhesive pads and near the digital pads. These regions are likely those most involved in the fine control of movements and response to vibrational stimuli derived from air and objects movements, including potential preys or predators.
MeSH term(s)	Animals ; Electrons ; Epidermis/metabolism ; Lizards/physiology ; beta-Keratins/metabolism ; Cell Differentiation ; Keratins/metabolism
Chemical Substances	beta-Keratins ; Keratins (68238-35-7)
Language	English
Publishing date	2022-10-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2269667-2
ISSN	1932-8494 ; 1932-8486
ISSN (online)	1932-8494
ISSN	1932-8486
DOI	10.1002/ar.25084
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Article ; Online: Morphology of setae in regenerating caudal adhesive pads of the gecko Lygodactylus capensis (Smith, 1849).

Alibardi, Lorenzo / Bonfitto, Antonio

Zoology (Jena, Germany)

2019 Volume 133, Page(s) 1–9

Abstract: After tail loss in the African gecko Lygodactylus capensis (Smith, 1949) a new tail is regenerated, including caudal adhesive pads. The axial skeleton of the regenerating tail consists in an elastic cartilaginous tube replacing the original vertebrae ... ...

Abstract	After tail loss in the African gecko Lygodactylus capensis (Smith, 1949) a new tail is regenerated, including caudal adhesive pads. The axial skeleton of the regenerating tail consists in an elastic cartilaginous tube replacing the original vertebrae that allows interacting with the substrate like in the original tail. The formation of adhesive setae has been analyzed using transmission and scanning electron microscopy coupled to immunolabeling for Corneous Beta Proteins. During progressive stages of epidermal differentiation new setae are developed at stage 4 of the shedding cycle and contain Corneous Beta Proteins. These structural proteins are faintly localized in the Oberhäutchen but are abundant in the beta-layer, indicating that the two epidermal layers have a different protein composition. The setae originate from the growth of Oberhäutchen spinulae into the cytoplasm of clear cells and the latter produce a thick fibrous meshwork of keratin and other unknown proteins localized around the growing setae. This cytoskeleton likely allows molding tail setae like for digital setae. A graded development of setae is observed from the base to the tip of regenerated pads and from the periphery to more central areas. The terminal end of the setae is subdivided into numerous filamentous spatulae that increase the adhesion contact. Sensory boutons are frequently detected at the margin of tail scales and adhesive pads, likely improving compliance with the substrate. The present study indicates that tail regeneration is a convenient experimental model to analyze adhesive setae formation, microstructures that allow to these lizards climbing vertical and arboreal substrates.
MeSH term(s)	Animals ; Biomechanical Phenomena ; Foot/anatomy & histology ; Lizards/physiology ; Regeneration/physiology ; Sensilla/physiology
Language	English
Publishing date	2019-02-01
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1191401-4
ISSN	1873-2720 ; 0944-2006
ISSN (online)	1873-2720
ISSN	0944-2006
DOI	10.1016/j.zool.2019.01.003
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Article ; Online: New Indo-Pacific species of the genus Teretia Norman, 1888 (Gastropoda: Raphitomidae).

Morassi, Mauro / Bonfitto, Antonio

Zootaxa

2015 Volume 3911, Issue 4, Page(s) 560–570

Abstract: Four new species are assigned to the genus Teretia Norman, 1888 in the family Raphitomidae Bellardi, 1875 and herein described: Teretia neocaledonica sp. nov., T. sysoevi sp. nov., T. tongaensis sp. nov. from the southeastern Pacific and Teretia tavianii ...

Abstract	Four new species are assigned to the genus Teretia Norman, 1888 in the family Raphitomidae Bellardi, 1875 and herein described: Teretia neocaledonica sp. nov., T. sysoevi sp. nov., T. tongaensis sp. nov. from the southeastern Pacific and Teretia tavianii sp. nov. from the Gulf of Aden. The new species represent the first Indo-Pacific record of a genus previously known in the recent molluscan fauna by only two species from the Atlantic Ocean-Mediterranean Sea and Southern Africa. A possible Tethyan origin for the genus Teretia is suggested.
MeSH term(s)	Animal Distribution ; Animal Structures/anatomy & histology ; Animal Structures/growth & development ; Animals ; Atlantic Ocean ; Body Size ; Gastropoda/anatomy & histology ; Gastropoda/classification ; Gastropoda/growth & development ; Indian Ocean ; Organ Size
Language	English
Publishing date	2015-01-21
Publishing country	New Zealand
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.3911.4.5
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Article: Three new bathyal raphitomine gastropods (Mollusca: Conoidea) from the Indo-Pacific region.

Morassi, Mauro / Bonfitto, Antonio

Zootaxa

2015 Volume 3620, Page(s) 579–588

Abstract: Three new species of Conoidea are described from Red Sea, Gulf of Aden and Philippines. Awheaturris lozoueti sp. nov., from Philippines, is the first representative in the recent Indo-Pacific molluscan fauna of a hitherto Miocene fossil genus. Taranis ... ...

Abstract	Three new species of Conoidea are described from Red Sea, Gulf of Aden and Philippines. Awheaturris lozoueti sp. nov., from Philippines, is the first representative in the recent Indo-Pacific molluscan fauna of a hitherto Miocene fossil genus. Taranis adenensis sp. nov., from Gulf of Aden, is the first species certainly referable to genus Taranis Jeffreys, 1870 reported in the Gulf of Aden and the smallest described member of this genus in the Indo-Pacific region. Mioawateria vivens sp. nov. represents the first member of the genus Mioawateria Vella, 1954 reported in the Red Sea. The status of Mioawateria is discussed and photographs of its type species, Awateria (Mioawateria) personata Powell, 1942, from the Pliocene of New Zealand, are presented for the first time.
MeSH term(s)	Animals ; Fossils/anatomy & histology ; Indian Ocean ; New Zealand ; Philippines ; Snails/anatomy & histology ; Snails/classification
Language	English
Publishing date	2015-06-22
Publishing country	New Zealand
Document type	Journal Article
ISSN	1175-5326
ISSN	1175-5326
DOI	10.11646/zootaxa.3620.4.7
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Article: Four new African turriform gastropods (Mollusca: Conoidea).

Morassi, Mauro / Bonfitto, Antonio

Zootaxa

2015 Volume 3710, Page(s) 271–280

Abstract: Four new species, belonging to four distinct conoidean families, are described from east Africa and Mozambique Channel. Iredalea adenensis sp. nov. (Drilliidae Olsson, 1964), from Gulf of Aden, and Buchema shearmani sp. nov. (Horaiclavidae Bouchet et al., ...

Abstract	Four new species, belonging to four distinct conoidean families, are described from east Africa and Mozambique Channel. Iredalea adenensis sp. nov. (Drilliidae Olsson, 1964), from Gulf of Aden, and Buchema shearmani sp. nov. (Horaiclavidae Bouchet et al., 2011), from off Mogadishu (Somalia), both trawled by local fishermen, represent the first record of their respective genera in eastern Africa. Crassispira somalica sp. nov. (Pseudomelatomidae Morrison, 1965), also collected offshore from Modagishu (Somalia), represents the first eastern Africa species bearing "typical" Crassispira features. Tropidoturris vizcondei sp. nov. (Borsoniidae Bellardi, 1875), from the Mozambique Channel, increases the knowledge of a genus considered endemic to southeastern Africa.
MeSH term(s)	Africa, Eastern ; Animals ; Biodiversity ; Gastropoda/anatomy & histology ; Gastropoda/classification
Language	English
Publishing date	2015-06-02
Publishing country	New Zealand
Document type	Journal Article
ISSN	1175-5326
ISSN	1175-5326
DOI	10.11646/zootaxa.3710.3.5
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Article ; Online: Two new Horaiclavus (Horaiclavidae, Conoidea) species from the Indo-Pacific region.

Bonfitto, Antonio / Morassi, Mauro

Zootaxa

2014 Volume 3821, Issue 1, Page(s) 146–150

Abstract: The genus Horaiclavus includes eight Holocene Indo-Pacific species (Appeltans et al. 2012). Herein, we describe two new species that resemble members of this genus in some aspects of shell morphology, but otherwise show features that suggest that they ... ...

Abstract	The genus Horaiclavus includes eight Holocene Indo-Pacific species (Appeltans et al. 2012). Herein, we describe two new species that resemble members of this genus in some aspects of shell morphology, but otherwise show features that suggest that they differ from "typical" Horaiclavus species. Descriptions and measurements were based on shells oriented spire up with the aperture facing the viewer and made using a Leica MS 5 Stereomicroscope for incident light and a 10× ocular micrometer. Scanning electron microscope (SEM) micrographs were taken using a Hitachi S-2400. The classification adopted here follows Bouchet et al. (2011). The following abbreviations are used in the text: MZB = Museo di Zoologia dell'Università di Bologna; MNHN = Muséum National d'Histoire Naturelle, Paris, France; NHMUK = The Natural History Museum of United Kingdom [formerly British Museum (Natural History)], London, United Kingdom; ZMA = Naturalis Biodiversity Center, Leiden, the Netherlands; ZRC = Zoological Reference Collection, Raffles Museum of Biodiversity Research, Singapore; a = aperture length; b = shell width; l = length; a/l = ratio of aperture length to total shell length; b/l = ratio of shell breadth to total length; dd= dead collected specimen(s); stn. = station.
MeSH term(s)	Animal Structures/anatomy & histology ; Animal Structures/growth & development ; Animals ; Body Size ; Gastropoda/anatomy & histology ; Gastropoda/classification ; Gastropoda/growth & development ; Indian Ocean ; Organ Size ; Pacific Ocean
Language	English
Publishing date	2014-06-19
Publishing country	New Zealand
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.3821.1.12
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Article: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics.

Muscarella, Lucia Anna / Fabrizio, Federico Pio / De Bonis, Maria / Mancini, Maria Teresa / Balsamo, Teresa / Graziano, Paolo / Centra, Flavia / Sparaneo, Angelo / Trombetta, Domenico / Bonfitto, Antonio / Scagliusi, Vito / Larizza, Pietro / Capoluongo, Ettore Domenico / Fazio, Vito Michele

Cancers

2019 Volume 11, Issue 11

Abstract: Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When ... ...

Abstract	Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When considering clinical applications of NGS in oncology, a rapid workflow for DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples, as well as producing high quality library preparation, can be real challenges. Here we consider these targets and how applying effective automation technology to NGS workflows may help improve yield, timing and quality-control. We firstly evaluated DNA recovery from archived FFPE blocks from three different manual extraction methods and two automated extraction workstations. The workflow was then implemented to somatic (lung/colon panel) and germline (
Language	English
Publishing date	2019-10-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers11111691
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Article ; Online: Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study.

Malapelle, Umberto / Parente, Paola / Pepe, Francesco / De Luca, Caterina / Cerino, Pellegrino / Covelli, Claudia / Balestrieri, Mariangela / Russo, Gianluca / Bonfitto, Antonio / Pisapia, Pasquale / Fiordelisi, Fabiola / D'Armiento, Maria / Bruzzese, Dario / Loupakis, Fotios / Pietrantonio, Filippo / Triassi, Maria / Fassan, Matteo / Troncone, Giancarlo / Graziano, Paolo

Cells

2020 Volume 9, Issue 9

Abstract: Immunohistochemistry (IHC) and polymerase chain reaction (PCR) and fragment separation by capillary electrophoresis represent the current clinical laboratory standard for the evaluation of microsatellite instability (MSI) status. The importance of ... ...

Abstract	Immunohistochemistry (IHC) and polymerase chain reaction (PCR) and fragment separation by capillary electrophoresis represent the current clinical laboratory standard for the evaluation of microsatellite instability (MSI) status. The importance of reporting MSI status in colorectal cancer is based on its potential for guiding treatment and as a prognostic indicator. It is also used to identify patients for Lynch syndrome testing. Our aim was to evaluate pre-analytical factors, such as age of formalin-fixed and paraffin-embedded (FFPE) block, neoplastic cell percentage, mucinous component, and DNA integrity, that may influence the accuracy of MSI testing and assess the concordance between three different MSI evaluation approaches. We selected the mucinous colorectal cancer (CRC) histotype for this study as it may possibly represent an intrinsic diagnostic issue due to its low tumor cellularity. Seventy-five cases of mucinous CRC and corresponding normal colon tissue samples were retrospectively selected. MMR proteins were evaluated by IHC. After DNA quality and quantity evaluation, the Idylla™ and TapeStation 4200 platforms were adopted for the evaluation of MSI status. Seventy-three (97.3%) cases were successfully analyzed by the three methodologies. Overall, the Idylla™ platform showed a concordance rate with IHC of 98.0% for microsatellite stable (MSS)/proficient MMR (pMMR) cases and 81.8% for MSI/deficient MMR (dMMR) cases. The TapeStation 4200 system showed a concordance rate with IHC of 96.0% for MSS/pMMR cases and 45.4% for MSI/dMMR cases. The concordance rates of the TapeStation 4200 system with respect to the Idylla™ platform were 98.1% for MSS profile and 57.8% for MSI profile. Discordant cases were analyzed using the Titano MSI kit. Considering pre-analytical factors, no significant variation in concordance rate among IHC analyses and molecular systems was observed by considering the presence of an acellular mucus cut-off >50% of the tumor area, FFPE year preparation, and DNA concentration. Conversely, the Idylla™ platform showed a significant variation in concordance rate with the IHC approach by considering a neoplastic cell percentage >50% (
MeSH term(s)	Adenocarcinoma, Mucinous/diagnosis ; Adenocarcinoma, Mucinous/genetics ; Adenocarcinoma, Mucinous/pathology ; Aged ; Case-Control Studies ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Colorectal Neoplasms, Hereditary Nonpolyposis/diagnosis ; Colorectal Neoplasms, Hereditary Nonpolyposis/genetics ; Colorectal Neoplasms, Hereditary Nonpolyposis/pathology ; DNA, Neoplasm/genetics ; DNA, Neoplasm/metabolism ; Diagnosis, Differential ; Electrophoresis, Capillary/standards ; Female ; Humans ; Immunohistochemistry/standards ; Male ; Microsatellite Instability ; Middle Aged ; Polymerase Chain Reaction/standards ; Prognosis ; Retrospective Studies ; Tissue Embedding/methods ; Tissue Embedding/standards ; Tissue Fixation/methods ; Tissue Fixation/standards
Chemical Substances	DNA, Neoplasm
Language	English
Publishing date	2020-09-02
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells9092019
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Article ; Online: Effects of KEAP1 Silencing on the Regulation of NRF2 Activity in Neuroendocrine Lung Tumors.

International journal of molecular sciences

2019 Volume 20, Issue 10

Abstract: Background: The KEAP1/NRF2 pathway has been widely investigated in tumors since it was implicated in cancer cells survival and therapies resistance. In lung tumors the deregulation of this pathway is mainly related to point mutations of : Methods: ... ...

Abstract	Background: The KEAP1/NRF2 pathway has been widely investigated in tumors since it was implicated in cancer cells survival and therapies resistance. In lung tumors the deregulation of this pathway is mainly related to point mutations of Methods: The effects of Results: We demonstrated that in carcinoid cell lines, the Conclusions: This is the first study that has described the effects of
MeSH term(s)	Adult ; Cell Line, Tumor ; DNA Methylation ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Kelch-Like ECH-Associated Protein 1/genetics ; Lung Neoplasms/genetics ; Lung Neoplasms/pathology ; Male ; Middle Aged ; NF-E2-Related Factor 2/genetics ; Neuroendocrine Tumors/genetics ; Neuroendocrine Tumors/pathology ; Young Adult
Chemical Substances	KEAP1 protein, human ; Kelch-Like ECH-Associated Protein 1 ; NF-E2-Related Factor 2 ; NFE2L2 protein, human
Language	English
Publishing date	2019-05-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20102531
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