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  1. Article ; Online: Complete and Circularized Genome Sequences of Three

    Ruh, Mylène / Briand, Martial / Bonneau, Sophie / Darrasse, Armelle / Jacques, Marie-Agnès / Chen, Nicolas W G

    Microbiology resource announcements

    2021  Volume 10, Issue 31, Page(s) e0053721

    Abstract: We report the complete and circularized genome sequences of two strains of Xanthomonas citri pv. glycines causing bacterial pustule on soybean and one strain of Xanthomonas euvesicatoria pv. alfalfae causing bacterial leaf and stem spot on alfalfa. These ...

    Abstract We report the complete and circularized genome sequences of two strains of Xanthomonas citri pv. glycines causing bacterial pustule on soybean and one strain of Xanthomonas euvesicatoria pv. alfalfae causing bacterial leaf and stem spot on alfalfa. These assemblies provide high-quality material for functional and evolutionary studies of these legume pathogens.
    Language English
    Publishing date 2021-08-05
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00537-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Xanthomonas adaptation to common bean is associated with horizontal transfers of genes encoding TAL effectors.

    Ruh, Mylène / Briand, Martial / Bonneau, Sophie / Jacques, Marie-Agnès / Chen, Nicolas W G

    BMC genomics

    2017  Volume 18, Issue 1, Page(s) 670

    Abstract: Background: Common bacterial blight is a devastating bacterial disease of common bean (Phaseolus vulgaris) caused by Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli. These phylogenetically distant strains are able to cause similar ... ...

    Abstract Background: Common bacterial blight is a devastating bacterial disease of common bean (Phaseolus vulgaris) caused by Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli. These phylogenetically distant strains are able to cause similar symptoms on common bean, suggesting that they have acquired common genetic determinants of adaptation to common bean. Transcription Activator-Like (TAL) effectors are bacterial type III effectors that are able to induce the expression of host genes to promote infection or resistance. Their capacity to bind to a specific host DNA sequence suggests that they are potential candidates for host adaption.
    Results: To study the diversity of tal genes from Xanthomonas strains responsible for common bacterial blight of bean, whole genome sequences of 17 strains representing the diversity of X. citri pv. fuscans and X. phaseoli pv. phaseoli were obtained by single molecule real time sequencing. Analysis of these genomes revealed the existence of four tal genes named tal23A, tal20F, tal18G and tal18H, respectively. While tal20F and tal18G were chromosomic, tal23A and tal18H were carried on plasmids and shared between phylogenetically distant strains, therefore suggesting recent horizontal transfers of these genes between X. citri pv. fuscans and X. phaseoli pv. phaseoli strains. Strikingly, tal23A was present in all strains studied, suggesting that it played an important role in adaptation to common bean. In silico predictions of TAL effectors targets in the common bean genome suggested that TAL effectors shared by X. citri pv. fuscans and X. phaseoli pv. phaseoli strains target the promoters of genes of similar functions. This could be a trace of convergent evolution among TAL effectors from different phylogenetic groups, and comforts the hypothesis that TAL effectors have been implied in the adaptation to common bean.
    Conclusions: Altogether, our results favour a model where plasmidic TAL effectors are able to contribute to host adaptation by being horizontally transferred between distant lineages.
    Language English
    Publishing date 2017-08-30
    Publishing country England
    Document type Journal Article
    ISSN 1471-2164
    ISSN (online) 1471-2164
    DOI 10.1186/s12864-017-4087-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: First Complete Genome Sequences of

    Ruh, Mylène / Briand, Martial / Bonneau, Sophie / Jacques, Marie-Agnès / Chen, Nicolas W G

    Genome announcements

    2017  Volume 5, Issue 36

    Abstract: Xanthomonas ... ...

    Abstract Xanthomonas citri
    Language English
    Publishing date 2017-09-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00813-17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Functional Microbial Features Driving Community Assembly During Seed Germination and Emergence.

    Torres-Cortés, Gloria / Bonneau, Sophie / Bouchez, Olivier / Genthon, Clémence / Briand, Martial / Jacques, Marie-Agnès / Barret, Matthieu

    Frontiers in plant science

    2018  Volume 9, Page(s) 902

    Abstract: Microbial interactions occurring on and around seeds are especially important for plant fitness since seed-borne microorganisms are the initial source of inoculum for the plant microbiota. In this study, we analyze structural and functional changes ... ...

    Abstract Microbial interactions occurring on and around seeds are especially important for plant fitness since seed-borne microorganisms are the initial source of inoculum for the plant microbiota. In this study, we analyze structural and functional changes occurring within the plant microbiota at these early stages of the plant cycle, namely germination and emergence. To this purpose, we performed shotgun DNA sequencing of microbial assemblages associated to seeds, germinating seeds and seedlings of two plant species: bean and radish. We observed an enrichment of
    Language English
    Publishing date 2018-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2018.00902
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing.

    Fischer-Le Saux, Marion / Bonneau, Sophie / Essakhi, Salwa / Manceau, Charles / Jacques, Marie-Agnès

    Applied and environmental microbiology

    2015  Volume 81, Issue 14, Page(s) 4651–4668

    Abstract: Deep and comprehensive knowledge of the genetic structure of pathogenic species is the cornerstone on which the design of precise molecular diagnostic tools is built. Xanthomonas arboricola is divided into pathovars, some of which are classified as ... ...

    Abstract Deep and comprehensive knowledge of the genetic structure of pathogenic species is the cornerstone on which the design of precise molecular diagnostic tools is built. Xanthomonas arboricola is divided into pathovars, some of which are classified as quarantine organisms in many countries and are responsible for diseases on nut and stone fruit trees that have emerged worldwide. Recent taxonomic studies of the genus Xanthomonas showed that strains isolated from other hosts should be classified in X. arboricola, extending the host range of the species. To investigate the genetic structure of X. arboricola and the genetic relationships between highly pathogenic strains and strains apparently not relevant to plant health, we conducted multilocus sequence analyses on a collection of strains representative of the known diversity of the species. Most of the pathovars were clustered in separate monophyletic groups. The pathovars pruni, corylina, and juglandis, responsible for pandemics in specific hosts, were highly phylogenetically related and clustered in three distinct clonal complexes. In contrast, strains with no or uncertain pathogenicity were represented by numerous unrelated singletons scattered in the phylogenic tree. Depending on the pathovar, intra- and interspecies recombination played contrasting roles in generating nucleotide polymorphism. This work provides a population genetics framework for molecular epidemiological surveys of emerging plant pathogens within X. arboricola. Based on our results, we propose to reclassify three former pathovars of Xanthomonas campestris as X. arboricola pv. arracaciae comb. nov., X. arboricola pv. guizotiae comb. nov., and X. arboricola pv. zantedeschiae comb. nov. An emended description of X. arboricola Vauterin et al. 1995 is provided.
    MeSH term(s) Host Specificity ; Molecular Sequence Data ; Multilocus Sequence Typing ; Plant Diseases/microbiology ; Plants/classification ; Plants/microbiology ; Xanthomonas/classification ; Xanthomonas/genetics ; Xanthomonas/isolation & purification ; Xanthomonas/physiology
    Language English
    Publishing date 2015-05-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.00050-15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Terroir is a key driver of seed‐associated microbial assemblages

    Klaedtke, Stephanie / Jacques, Marie‐Agnès / Raggi, Lorenzo / Préveaux, Anne / Bonneau, Sophie / Negri, Valeria / Chable, Véronique / Barret, Matthieu

    Environmental microbiology. 2016 June, v. 18, no. 6

    2016  

    Abstract: Seeds have evolved in association with diverse microbial assemblages that may influence plant growth and health. However, little is known about the composition of seed‐associated microbial assemblages and the ecological processes shaping their structures. ...

    Abstract Seeds have evolved in association with diverse microbial assemblages that may influence plant growth and health. However, little is known about the composition of seed‐associated microbial assemblages and the ecological processes shaping their structures. In this work, we monitored the relative influence of the host genotypes and terroir on the structure of the seed microbiota through metabarcoding analysis of different microbial assemblages associated to five different bean cultivars harvested in two distinct farms. Overall, few bacterial and fungal operational taxonomic units (OTUs) were conserved across all seed samples. The lack of shared OTUs between samples is explained by a significant effect of the farm site on the structure of microbial assemblage, which explained 12.2% and 39.7% of variance in bacterial and fungal diversity across samples. This site‐specific effect is reflected by the significant enrichment of 70 OTUs in Brittany and 88 OTUs in Luxembourg that lead to differences in co‐occurrence patterns. In contrast, variance in microbial assemblage structure was not explained by host genotype. Altogether, these results suggest that seed‐associated microbial assemblage is determined by niche‐based processes and that the terroir is a key driver of these selective forces.
    Keywords beans ; cultivars ; farms ; fungi ; genotype ; plant growth ; seeds ; variance ; France ; Luxembourg
    Language English
    Dates of publication 2016-06
    Size p. 1792-1804.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.12977
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Xanthomonas citri

    Ferreira, Marisa A S V / Bonneau, Sophie / Briand, Martial / Cesbron, Sophie / Portier, Perrine / Darrasse, Armelle / Gama, Marco A S / Barbosa, Maria Angélica G / Mariano, Rosa de L R / Souza, Elineide B / Jacques, Marie-Agnès

    Frontiers in plant science

    2019  Volume 10, Page(s) 489

    Abstract: ... The ... ...

    Abstract The pathovar
    Language English
    Publishing date 2019-04-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711035-7
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2019.00489
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Terroir is a key driver of seed-associated microbial assemblages.

    Klaedtke, Stephanie / Jacques, Marie-Agnès / Raggi, Lorenzo / Préveaux, Anne / Bonneau, Sophie / Negri, Valeria / Chable, Véronique / Barret, Matthieu

    Environmental microbiology

    2016  Volume 18, Issue 6, Page(s) 1792–1804

    Abstract: Seeds have evolved in association with diverse microbial assemblages that may influence plant growth and health. However, little is known about the composition of seed-associated microbial assemblages and the ecological processes shaping their structures. ...

    Abstract Seeds have evolved in association with diverse microbial assemblages that may influence plant growth and health. However, little is known about the composition of seed-associated microbial assemblages and the ecological processes shaping their structures. In this work, we monitored the relative influence of the host genotypes and terroir on the structure of the seed microbiota through metabarcoding analysis of different microbial assemblages associated to five different bean cultivars harvested in two distinct farms. Overall, few bacterial and fungal operational taxonomic units (OTUs) were conserved across all seed samples. The lack of shared OTUs between samples is explained by a significant effect of the farm site on the structure of microbial assemblage, which explained 12.2% and 39.7% of variance in bacterial and fungal diversity across samples. This site-specific effect is reflected by the significant enrichment of 70 OTUs in Brittany and 88 OTUs in Luxembourg that lead to differences in co-occurrence patterns. In contrast, variance in microbial assemblage structure was not explained by host genotype. Altogether, these results suggest that seed-associated microbial assemblage is determined by niche-based processes and that the terroir is a key driver of these selective forces.
    MeSH term(s) Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Ecosystem ; Fungi/classification ; Fungi/genetics ; Fungi/isolation & purification ; Microbiota ; Seeds/microbiology
    Language English
    Publishing date 2016-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.12977
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean.

    Chen, Nicolas W G / Serres-Giardi, Laurana / Ruh, Mylène / Briand, Martial / Bonneau, Sophie / Darrasse, Armelle / Barbe, Valérie / Gagnevin, Lionel / Koebnik, Ralf / Jacques, Marie-Agnès

    BMC genomics

    2018  Volume 19, Issue 1, Page(s) 606

    Abstract: Background: Host specialization is a hallmark of numerous plant pathogens including bacteria, fungi, oomycetes and viruses. Yet, the molecular and evolutionary bases of host specificity are poorly understood. In some cases, pathological convergence is ... ...

    Abstract Background: Host specialization is a hallmark of numerous plant pathogens including bacteria, fungi, oomycetes and viruses. Yet, the molecular and evolutionary bases of host specificity are poorly understood. In some cases, pathological convergence is observed for individuals belonging to distant phylogenetic clades. This is the case for Xanthomonas strains responsible for common bacterial blight of bean, spread across four genetic lineages. All the strains from these four lineages converged for pathogenicity on common bean, implying possible gene convergences and/or sharing of a common arsenal of genes conferring the ability to infect common bean.
    Results: To search for genes involved in common bean specificity, we used a combination of whole-genome analyses without a priori, including a genome scan based on k-mer search. Analysis of 72 genomes from a collection of Xanthomonas pathovars unveiled 115 genes bearing DNA sequences specific to strains responsible for common bacterial blight, including 20 genes located on a plasmid. Of these 115 genes, 88 were involved in successive events of horizontal gene transfers among the four genetic lineages, and 44 contained nonsynonymous polymorphisms unique to the causal agents of common bacterial blight.
    Conclusions: Our study revealed that host specificity of common bacterial blight agents is associated with a combination of horizontal transfers of genes, and highlights the role of plasmids in these horizontal transfers.
    MeSH term(s) Bacterial Proteins/genetics ; Gene Transfer, Horizontal ; Genome, Bacterial ; Host-Pathogen Interactions ; Phaseolus/genetics ; Phaseolus/growth & development ; Phaseolus/microbiology ; Phylogeny ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA ; Virulence ; Whole Genome Sequencing ; Xanthomonas/classification ; Xanthomonas/pathogenicity
    Chemical Substances Bacterial Proteins
    Language English
    Publishing date 2018-08-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-018-4975-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System.

    Essakhi, Salwa / Cesbron, Sophie / Fischer-Le Saux, Marion / Bonneau, Sophie / Jacques, Marie-Agnès / Manceau, Charles

    Applied and environmental microbiology

    2015  Volume 81, Issue 16, Page(s) 5395–5410

    Abstract: Xanthomonas arboricola is conventionally known as a taxon of plant-pathogenic bacteria that includes seven pathovars. This study showed that X. arboricola also encompasses nonpathogenic bacteria that cause no apparent disease symptoms on their hosts. The ...

    Abstract Xanthomonas arboricola is conventionally known as a taxon of plant-pathogenic bacteria that includes seven pathovars. This study showed that X. arboricola also encompasses nonpathogenic bacteria that cause no apparent disease symptoms on their hosts. The aim of this study was to assess the X. arboricola population structure associated with walnut, including nonpathogenic strains, in order to gain a better understanding of the role of nonpathogenic xanthomonads in walnut microbiota. A multilocus sequence analysis (MLSA) was performed on a collection of 100 X. arboricola strains, including 27 nonpathogenic strains isolated from walnut. Nonpathogenic strains grouped outside clusters defined by pathovars and formed separate genetic lineages. A multilocus variable-number tandem-repeat analysis (MLVA) conducted on a collection of X. arboricola strains isolated from walnut showed that nonpathogenic strains clustered separately from clonal complexes containing Xanthomonas arboricola pv. juglandis strains. Some nonpathogenic strains of X. arboricola did not contain the canonical type III secretion system (T3SS) and harbored only one to three type III effector (T3E) genes. In the nonpathogenic strains CFBP 7640 and CFBP 7653, neither T3SS genes nor any of the analyzed T3E genes were detected. This finding raises a question about the origin of nonpathogenic strains and the evolution of plant pathogenicity in X. arboricola. T3E genes that were not detected in any nonpathogenic isolates studied represent excellent candidates to be those responsible for pathogenicity in X. arboricola.
    MeSH term(s) Cluster Analysis ; Genotype ; Juglans/microbiology ; Minisatellite Repeats ; Molecular Sequence Data ; Multilocus Sequence Typing ; Phylogeny ; Plant Diseases/microbiology ; Sequence Homology ; Type III Secretion Systems/genetics ; Xanthomonas/classification ; Xanthomonas/genetics ; Xanthomonas/isolation & purification
    Chemical Substances Type III Secretion Systems
    Language English
    Publishing date 2015-06-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.00835-15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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