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Article ; Online: SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response.

Majoros, Hajnalka / Borsos, Barbara N / Ujfaludi, Zsuzsanna / Páhi, Zoltán G / Mórocz, Mónika / Haracska, Lajos / Boros, Imre Miklós / Pankotai, Tibor

International journal of molecular sciences

2021 Volume 22, Issue 16

Abstract: UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the ... ...

Abstract	UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To identify new players in UV-induced repair, we performed a microarray screen, in which we found
MeSH term(s)	Cell Line, Tumor ; DNA Damage ; DNA Repair/radiation effects ; Humans ; S Phase/radiation effects ; Serpins/genetics ; Serpins/metabolism ; Ultraviolet Rays/adverse effects
Chemical Substances	SERPINB10 protein, human ; Serpins
Language	English
Publishing date	2021-08-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22168500
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Article ; Online: Biological activity of green-synthesized silver nanoparticles depends on the applied natural extracts: a comprehensive study.

Rónavári, Andrea / Kovács, Dávid / Igaz, Nóra / Vágvölgyi, Csaba / Boros, Imre Miklós / Kónya, Zoltán / Pfeiffer, Ilona / Kiricsi, Mónika

International journal of nanomedicine

2017 Volume 12, Page(s) 871–883

Abstract: Due to obvious disadvantages of the classical chemical methods, green synthesis of metallic nanoparticles has attracted tremendous attention in recent years. Numerous environmentally benign synthesis methods have been developed yielding nanoparticles via ...

Abstract	Due to obvious disadvantages of the classical chemical methods, green synthesis of metallic nanoparticles has attracted tremendous attention in recent years. Numerous environmentally benign synthesis methods have been developed yielding nanoparticles via low-cost, eco-friendly, and simple approaches. In this study, our aim was to determine the suitability of coffee and green tea extracts in green synthesis of silver nanoparticles as well as to compare the performance of the obtained materials in different biological systems. We successfully produced silver nanoparticles (C-AgNP and GT-AgNP) using coffee and green tea extracts; moreover, based on our comprehensive screening, we delineated major differences in the biological activity of C-AgNPs and GT-AgNPs. Our results indicate that although GT-AgNPs exhibited excellent antimicrobial activity against all the examined microbial pathogens, these particles were also highly toxic to mammalian cells, which limits their potential applications. On the contrary, C-AgNPs manifested substantial inhibitory action on the tested microbes but were nontoxic to human and mouse cells, indicating an outstanding capacity to discriminate between potential pathogens and mammalian cells. These results clearly show that the various green materials used for stabilization and for reduction of metal ions have a defining role in determining and fine-tuning the biological activity of the obtained nanoparticles.
MeSH term(s)	Animals ; Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacology ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Bacteria/drug effects ; Cell Proliferation/drug effects ; Coffee/chemistry ; Fungi/drug effects ; HeLa Cells ; Humans ; Metal Nanoparticles/chemistry ; Mice ; NIH 3T3 Cells ; Plant Extracts/pharmacology ; Silver/chemistry ; Tea
Chemical Substances	Anti-Infective Agents ; Antineoplastic Agents ; Antioxidants ; Coffee ; Plant Extracts ; Tea ; Silver (3M4G523W1G)
Language	English
Publishing date	2017
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2364941-0
ISSN	1178-2013 ; 1176-9114
ISSN (online)	1178-2013
ISSN	1176-9114
DOI	10.2147/IJN.S122842
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Article ; Online: Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells.

Gopisetty, Mohana Krishna / Kovács, Dávid / Igaz, Nóra / Rónavári, Andrea / Bélteky, Péter / Rázga, Zsolt / Venglovecz, Viktória / Csoboz, Bálint / Boros, Imre Miklós / Kónya, Zoltán / Kiricsi, Mónika

Journal of nanobiotechnology

2019 Volume 17, Issue 1, Page(s) 9

Abstract: Background: Development of multidrug resistance (MDR) is a major burden of successful chemotherapy, therefore, novel approaches to defeat MDR are imperative. Although the remarkable anti-cancer propensity of silver nanoparticles (AgNP) has been ... ...

Abstract	Background: Development of multidrug resistance (MDR) is a major burden of successful chemotherapy, therefore, novel approaches to defeat MDR are imperative. Although the remarkable anti-cancer propensity of silver nanoparticles (AgNP) has been demonstrated and their potential application in MDR cancer has been proposed, the nanoparticle size-dependent cellular events directing P-glycoprotein (Pgp) expression and activity in MDR cancer have never been addressed. Hence, in the present study we examined AgNP size-dependent cellular features in multidrug resistant breast cancer cells. Results: In this study we report that 75 nm AgNPs inhibited significantly Pgp efflux activity in drug-resistant breast cancer cells and potentiated the apoptotic effect of doxorubicin, which features were not observed upon 5 nm AgNP treatment. Although both sized AgNPs induced significant ROS production and mitochondrial damage, 5 nm AgNPs were more potent than 75 nm AgNPs in this respect, therefore, these effects can not to be accounted for the reduced transport activity of ATP-driven pumps observed after 75 nm AgNP treatments. Instead we found that 75 nm AgNPs depleted endoplasmic reticulum (ER) calcium stores, caused notable ER stress and decreased plasma membrane positioning of Pgp. Conclusion: Our study suggests that AgNPs are potent inhibitors of Pgp function and are promising agents for sensitizing multidrug resistant breast cancers to anticancer drugs. This potency is determined by their size, since 75 nm AgNPs are more efficient than smaller counterparts. This is a highly relevant finding as it renders AgNPs attractive candidates in rational design of therapeutically useful agents for tumor targeting. In the present study we provide evidence that exploitation of ER stress can be a propitious target in defeating multidrug resistance in cancers.
MeSH term(s)	ATP Binding Cassette Transporter, Subfamily B, Member 1/antagonists & inhibitors ; Antineoplastic Agents/therapeutic use ; Breast Neoplasms/drug therapy ; Drug Resistance, Multiple/drug effects ; Endoplasmic Reticulum/drug effects ; Endoplasmic Reticulum Stress/drug effects ; Female ; Humans ; MCF-7 Cells ; Metal Nanoparticles ; Particle Size ; Silver/pharmacology
Chemical Substances	ATP Binding Cassette Transporter, Subfamily B, Member 1 ; Antineoplastic Agents ; Silver (3M4G523W1G)
Language	English
Publishing date	2019-01-22
Publishing country	England
Document type	Journal Article
ISSN	1477-3155
ISSN (online)	1477-3155
DOI	10.1186/s12951-019-0448-4
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Article ; Online: SerpinB2 is involved in cellular response upon UV irradiation.

Majoros, Hajnalka / Ujfaludi, Zsuzsanna / Borsos, Barbara Nikolett / Hudacsek, Viktória Vivien / Nagy, Zita / Coin, Frederic / Buzas, Krisztina / Kovács, Ilona / Bíró, Tamás / Boros, Imre Miklós / Pankotai, Tibor

Scientific reports

2019 Volume 9, Issue 1, Page(s) 2753

Abstract: Ultraviolet light induced pyrimidine dimer is a helix distortion DNA damage type, which recruits repair complexes. However, proteins of these complexes that take part in both DNA damage recognition and repair have been well-described, the regulation of ... ...

Abstract	Ultraviolet light induced pyrimidine dimer is a helix distortion DNA damage type, which recruits repair complexes. However, proteins of these complexes that take part in both DNA damage recognition and repair have been well-described, the regulation of the downstream steps of nucleotide excision repair (NER) have not been clearly clarified yet. In a high-throughput screen, we identified SerpinB2 (SPB2) as one of the most dramatically upregulated gene in keratinocytes following UV irradiation. We found that both the mRNA and the protein levels of SPB2 were increased upon UV irradiation in various cell lines. Additionally, UV damage induced translocation of SPB2 from the cytoplasm to the nucleus as well as the damage induced foci formation of it. Here we show that SPB2 co-localizes with XPB involved in the NER pathway at UV-induced repair foci. Finally, we demonstrated that UV irradiation promoted the association of SPB2 with ubiquitylated proteins. In basal cell carcinoma tumour cells, we identified changes in the subcellular localization of SPB2. Based on our results, we conclude that SPB2 protein has a novel role in UV-induced NER pathway, since it regulates the removal of the repair complex from the damaged site leading to cancerous malformation.
MeSH term(s)	Bone Neoplasms/etiology ; Bone Neoplasms/pathology ; Carcinoma, Basal Cell/etiology ; Carcinoma, Basal Cell/pathology ; DNA Damage ; DNA Helicases/genetics ; DNA Helicases/metabolism ; DNA Repair ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Humans ; Melanoma/etiology ; Melanoma/pathology ; Osteosarcoma/etiology ; Osteosarcoma/pathology ; Plasminogen Activator Inhibitor 2/genetics ; Plasminogen Activator Inhibitor 2/metabolism ; Pyrimidine Dimers ; Tumor Cells, Cultured ; Ultraviolet Rays/adverse effects
Chemical Substances	DNA-Binding Proteins ; Plasminogen Activator Inhibitor 2 ; Pyrimidine Dimers ; XPBC-ERCC-3 protein (146045-44-5) ; DNA Helicases (EC 3.6.4.-)
Language	English
Publishing date	2019-02-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-39073-w
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Article ; Online: Genetic, epigenetic and transcriptional comparison of esophagus tumor‑associated and adjacent normal myofibroblasts.

Huliák, Ildikó / Bodai, László / Czepán, Mátyás / Kovács, Dávid / Szabó, Anikó / Tiszlavicz, László / Lázár, György / Rakonczay, Zoltán / Hegyi, Péter / Boros, Imre Miklós / Kiricsi, Mónika

Oncology reports

2018 Volume 41, Issue 2, Page(s) 839–852

Abstract: Myofibroblasts (MFs) are present in healthy tissues and are also key components of the tumor microenvironment. In the present study a comparative analysis of MFs obtained from various gastrointestinal tumor tissues and from tumor‑adjacent normal tissues ... ...

Abstract	Myofibroblasts (MFs) are present in healthy tissues and are also key components of the tumor microenvironment. In the present study a comparative analysis of MFs obtained from various gastrointestinal tumor tissues and from tumor‑adjacent normal tissues of cancer patients was performed, with the aim to evaluate differences in MF morphology, gene expression profile and function. The goal was to correlate the observed morphological and functional variations with the underlying genetic and epigenetic backgrounds. The mutation frequency of MFs was assessed by next generation sequencing. The transcript levels of cancer‑specific genes were determined by TaqMan array and quantitative polymerase chain reaction. Epigenetic modifications were analyzed by immunocytochemistry and western blotting. The migratory capacity of MFs was assessed by scratch assay, whereas matrix metalloproteinase expression and activity were obtained by quantitative polymerase chain reaction and zymography. The results of the present study demonstrate that MFs were present in an increased number and with altered morphology in tumor samples compared with the healthy tissue. Although the detected number of mutations in tumor‑associated and normal tissue‑derived MFs did not differ markedly, shifts in the level of specific acetylated and methylated histone proteins, namely decreased levels of trimethylated H3K9 and acetylated H4K16 were demonstrated in tumor‑associated MFs. Transcript levels of several tumor‑specific genes involved in metastasis, regulation of cellular growth, apoptosis, as well as in hypoxia‑angiogenesis were altered in tumor‑derived MF cultures. Increased mRNA levels were obtained and activity of matrix metalloproteases in tumor‑derived MFs and these cells also exhibited a higher migratory capacity compared with the normal MFs. In summary, the results of the present study indicate that tumor‑associated MFs display an altered phenotype compared with healthy tissue derived counterparts. The results imply that epigenetic rather than genetic alterations are associated with the development of the distinct expressional and functional features, which define this MF phenotype in the tumor microenvironment.
MeSH term(s)	Acetylation ; Aged ; Apoptosis/genetics ; Cell Proliferation/genetics ; DNA Methylation ; Epigenesis, Genetic ; Esophageal Neoplasms/genetics ; Esophageal Neoplasms/pathology ; Esophageal Neoplasms/surgery ; Esophagus/pathology ; Esophagus/surgery ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genes, Neoplasm/genetics ; Histones/genetics ; Histones/metabolism ; Humans ; Immunohistochemistry ; Male ; Myofibroblasts/metabolism ; Polymorphism, Genetic ; RNA, Messenger/metabolism ; Tumor Microenvironment/genetics
Chemical Substances	Histones ; RNA, Messenger
Language	English
Publishing date	2018-12-06
Publishing country	Greece
Document type	Comparative Study ; Journal Article
ZDB-ID	1222484-4
ISSN	1791-2431 ; 1021-335X
ISSN (online)	1791-2431
ISSN	1021-335X
DOI	10.3892/or.2018.6909
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Article ; Online: Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes.

Rónavári, Andrea / Igaz, Nóra / Gopisetty, Mohana Krishna / Szerencsés, Bettina / Kovács, Dávid / Papp, Csaba / Vágvölgyi, Csaba / Boros, Imre Miklós / Kónya, Zoltán / Kiricsi, Mónika / Pfeiffer, Ilona

International journal of nanomedicine

2018 Volume 13, Page(s) 695–703

Abstract: Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns ...

Abstract	Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns and providing much of the impetus for current attempts to develop novel and efficient agents against cutaneous mycosis causing species. Innovative, environmentally benign and economic nanotechnology-based approaches have recently emerged utilizing principally biological sources to produce nano-sized structures with unique antimicrobial properties. In line with this, our aim was to generate silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) by biological synthesis and to study the effect of the obtained nanoparticles on cutaneous mycosis causing fungi and on human keratinocytes. Methods: Cell-free extract of the red yeast Results: Antifungal studies demonstrated that the biosynthesized silver particles were able to inhibit the growth of several opportunistic Conclusion: Our results emphasize the therapeutic potential of such biosynthesized nanoparticles, since their biocompatibility to skin cells and their outstanding antifungal performance can be exploited for topical treatment and prophylaxis of superficial cutaneous mycosis.
MeSH term(s)	Antifungal Agents/metabolism ; Antifungal Agents/pharmacology ; Basidiomycota/metabolism ; Candida/drug effects ; Candida/pathogenicity ; Cell Line ; Cell-Free System ; Dermatomycoses/drug therapy ; Dermatomycoses/microbiology ; Drug Evaluation, Preclinical ; Dynamic Light Scattering ; Gold/chemistry ; Gold/pharmacology ; Humans ; Keratinocytes/drug effects ; Metal Nanoparticles/chemistry ; Metal Nanoparticles/therapeutic use ; Microscopy, Electron, Transmission ; Silver/chemistry ; Silver/pharmacology ; Trichophyton/drug effects ; Trichophyton/pathogenicity
Chemical Substances	Antifungal Agents ; Silver (3M4G523W1G) ; Gold (7440-57-5)
Language	English
Publishing date	2018-02-01
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2364941-0
ISSN	1178-2013 ; 1176-9114
ISSN (online)	1178-2013
ISSN	1176-9114
DOI	10.2147/IJN.S152010
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Article ; Online: Ecdysone induced gene expression is associated with acetylation of histone H3 lysine 23 in Drosophila melanogaster.

Bodai, László / Zsindely, Nóra / Gáspár, Renáta / Kristó, Ildikó / Komonyi, Orbán / Boros, Imre Miklós

PloS one

2012 Volume 7, Issue 7, Page(s) e40565

Abstract: Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone ... ...

Abstract	Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limited or no correlation with ecdysone induced transcriptional activity. We found that dCBP, which is encoded by the nejire gene, acetylates H3 lysine 23 in vivo, and silencing of nejire leads to reduced expression of the Eip74EF and Eip75B genes. Our results suggest that acetylation of specific lysine residues of histones contribute specifically to the dynamic regulation of transcription. Furthermore, along with previous studies identify CBP dependent H3 lysine 23 acetylation as an evolutionarily conserved chromatin modification involved in steroid induced gene activation.
MeSH term(s)	Acetylation/drug effects ; Acetyltransferases/metabolism ; Animals ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/drug effects ; Drosophila melanogaster/genetics ; Drosophila melanogaster/growth & development ; Ecdysone/pharmacology ; Gene Expression Regulation/drug effects ; Genes, Insect/genetics ; Histones/metabolism ; Larva/genetics ; Larva/growth & development ; Lysine/metabolism ; Models, Genetic ; Promoter Regions, Genetic/genetics ; Protein Processing, Post-Translational/drug effects ; Protein Processing, Post-Translational/genetics ; Time Factors ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcription, Genetic/drug effects ; p300-CBP Transcription Factors/metabolism
Chemical Substances	DNA-Binding Proteins ; Drosophila Proteins ; Eip74EF protein, Drosophila ; Histones ; Transcription Factors ; Eip75B protein, Drosophila (126968-14-7) ; Ecdysone (3604-87-3) ; Acetyltransferases (EC 2.3.1.-) ; nej protein, Drosophila (EC 2.3.1.48) ; p300-CBP Transcription Factors (EC 2.3.1.48) ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2012-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0040565
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Article: The dissociable RPB4 subunit of RNA Pol II has vital functions in Drosophila

Pankotai, Tibor / Újfaludi, Zsuzsanna / Vámos, Edith / Suri, Katalin / Boros, Imre Miklos

Molecular genetics and genomics MGG. 2010 Jan., v. 283, no. 1

2010

Abstract: RNA polymerase II (Pol II) is composed of a ten subunit core and a two subunit dissociable subcomplex comprising the fourth and seventh largest subunits, RPB4 and RPB7. The evolutionary highly conserved RPB4/7 heterodimer is positioned in the Pol II such ...

Abstract	RNA polymerase II (Pol II) is composed of a ten subunit core and a two subunit dissociable subcomplex comprising the fourth and seventh largest subunits, RPB4 and RPB7. The evolutionary highly conserved RPB4/7 heterodimer is positioned in the Pol II such that it can make contact with various factors involved in RNA biogenesis and is believed to play roles both during the process of transcription and post-transcription. A detailed analysis of RPB4/7 function in a multicellular eukaryote, however, is lacking partly because of the lack of a suitable genetic system. Here, we describe generation and initial analysis of Drosophila Rpb4 mutants. In the fly, RPB4 is a product of a bicistronic gene together with the ATAC histone acetyltransferase complex constituent ADA2a. DmAda2a and DmRpb4 are expressed during fly development at different levels. The structure of mature mRNA forms suggests that the production of DmADA2a and DmRPB4-specific mRNAs is ensured by alternative splicing. Genetic analysis indicates that both DmRPB4 and DmADA2a play essential roles, because their absence results in lethality in early and late larval stages, respectively. Upon stress of high temperature or nutritional starvation, the levels of RPB4 and ADA2a messages change differently. RPB4 colocalizes with Pol II to several sites on polytene chromosomes, however, at selected locus, the abundances of Pol II and RPB4 vary greatly. Our data suggest no tight functional link between DmADA2a and DmRPB4, and reveal differences in the abundances of Pol II core subunits and RPB4 localized at specific regions on polytene chromosomes, supporting the suggested role of RPB4 outside of transcription-engaged Pol II complexes.
Keywords	DNA-directed RNA polymerase ; Drosophila ; alternative splicing ; eukaryotic cells ; genes ; genetic techniques and protocols ; loci ; messenger RNA ; mutants ; polytene chromosomes ; starvation ; temperature
Language	English
Dates of publication	2010-01
Size	p. 89-97.
Publisher	Springer-Verlag
Publishing place	Berlin/Heidelberg
Document type	Article
ISSN	1617-4615
DOI	10.1007/s00438-009-0499-6
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The dissociable RPB4 subunit of RNA Pol II has vital functions in Drosophila.

Pankotai, Tibor / Ujfaludi, Zsuzsanna / Vámos, Edith / Suri, Katalin / Boros, Imre Miklos

Molecular genetics and genomics : MGG

2009 Volume 283, Issue 1, Page(s) 89–97

Abstract	RNA polymerase II (Pol II) is composed of a ten subunit core and a two subunit dissociable subcomplex comprising the fourth and seventh largest subunits, RPB4 and RPB7. The evolutionary highly conserved RPB4/7 heterodimer is positioned in the Pol II such that it can make contact with various factors involved in RNA biogenesis and is believed to play roles both during the process of transcription and post-transcription. A detailed analysis of RPB4/7 function in a multicellular eukaryote, however, is lacking partly because of the lack of a suitable genetic system. Here, we describe generation and initial analysis of Drosophila Rpb4 mutants. In the fly, RPB4 is a product of a bicistronic gene together with the ATAC histone acetyltransferase complex constituent ADA2a. DmAda2a and DmRpb4 are expressed during fly development at different levels. The structure of mature mRNA forms suggests that the production of DmADA2a and DmRPB4-specific mRNAs is ensured by alternative splicing. Genetic analysis indicates that both DmRPB4 and DmADA2a play essential roles, because their absence results in lethality in early and late larval stages, respectively. Upon stress of high temperature or nutritional starvation, the levels of RPB4 and ADA2a messages change differently. RPB4 colocalizes with Pol II to several sites on polytene chromosomes, however, at selected locus, the abundances of Pol II and RPB4 vary greatly. Our data suggest no tight functional link between DmADA2a and DmRPB4, and reveal differences in the abundances of Pol II core subunits and RPB4 localized at specific regions on polytene chromosomes, supporting the suggested role of RPB4 outside of transcription-engaged Pol II complexes.
MeSH term(s)	Animals ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/enzymology ; Drosophila melanogaster/genetics ; Histone Acetyltransferases/genetics ; Histone Acetyltransferases/metabolism ; Protein Subunits/genetics ; Protein Subunits/metabolism ; RNA Polymerase II/genetics ; RNA Polymerase II/metabolism ; Transcription, Genetic
Chemical Substances	Ada2a protein, Drosophila ; Drosophila Proteins ; Protein Subunits ; Histone Acetyltransferases (EC 2.3.1.48) ; RNA Polymerase II (EC 2.7.7.-) ; RPB4 protein, Drosophila (EC 2.7.7.-)
Language	English
Publishing date	2009-11-18
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2044817-X
ISSN	1617-4623 ; 1617-4615
ISSN (online)	1617-4623
ISSN	1617-4615
DOI	10.1007/s00438-009-0499-6
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