Article ; Online: Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples.
Journal of visualized experiments : JoVE
2023 , Issue 196
Abstract: Wastewater-based epidemiology has emerged as a promising and efficacious surveillance system for SARS-CoV-2 and other infectious diseases in many nations. The process typically involves wastewater concentration, nucleic acid extraction, amplification of ... ...
| Abstract | Wastewater-based epidemiology has emerged as a promising and efficacious surveillance system for SARS-CoV-2 and other infectious diseases in many nations. The process typically involves wastewater concentration, nucleic acid extraction, amplification of selected genomic segments, and detection and quantification of the amplified genomic segment. This methodology can similarly be leveraged to detect and quantify infectious agents, such as SARS-CoV-2, in air samples. Initially, SARS-CoV-2 was presumed to spread primarily through close personal contact with droplets generated by an infected individual while speaking, sneezing, coughing, singing, or breathing. However, a growing number of studies have reported the presence of SARS-CoV-2 RNA in the air of healthcare facilities, establishing airborne transmission as a viable route for the virus. This study presents a composite of established protocols to facilitate environmental detection, quantification, and sequencing of viruses from both wastewater and air samples. |
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| MeSH term(s) | Humans ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; Wastewater ; COVID-19 ; Nucleic Acids |
| Chemical Substances | RNA, Viral ; Wastewater ; Nucleic Acids |
| Language | English |
| Publishing date | 2023-06-30 |
| Publishing country | United States |
| Document type | Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2259946-0 |
| ISSN | 1940-087X ; 1940-087X |
| ISSN (online) | 1940-087X |
| ISSN | 1940-087X |
| DOI | 10.3791/65053 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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