Article ; Online: Passive transfer of affinity-purified anti-heart autoantibodies (AHA) from sera of patients with myocarditis induces experimental myocarditis in mice.
International journal of cardiology
2015 Volume 179, Page(s) 166–177
Abstract: Background: Human autoimmune myocarditis is characterized by an increased frequency of serum organ and disease-specific anti-heart autoantibodies (AHA) in affected patients. To assess whether AHA are directly pathogenic, we used the passive transfer ... ...
| Abstract | Background: Human autoimmune myocarditis is characterized by an increased frequency of serum organ and disease-specific anti-heart autoantibodies (AHA) in affected patients. To assess whether AHA are directly pathogenic, we used the passive transfer technique of AHA from patients to normal Balb/c mice to induce an experimental myocarditis. Methods: In keeping with a classical passive transfer experiment, sera from 5 AHA positive myocarditis patients (3 male, mean age 30 ± 11 years, 3 with giant cell and 2 with lymphocytic myocarditis) were affinity purified and injected into 25 Balb/c mice. As controls, affinity purified sera from 5 healthy donors were passively transferred to 25 Balb/c mice. Further 15 control mice were injected with phosphate-buffered saline and 9 mice did not receive any injection. In all patients cardiac-specific AHA of IgG class had been previously detected by an indirect immunofluorescence (IFL) technique on cryostat sections of O blood group human heart. The animals were sacrificed after 4 weeks and the hearts were blindly examined for histological evidence of myocarditis by an expert cardiac pathologist. Results: Myocarditis was present in 13/25 (52%) of the mice which received affinity-purified IgG from patients. The findings of severe, moderate or mild myocarditis were more common in the mice which received affinity-purified IgG from patients (20%; 20% and 12%) than in control animals (2%, p=0.01; 0%, p=0.003; and 0%, p=0.04 respectively). Conclusions: These findings provide a new evidence for AHA-mediated pathogenicity in human myocarditis, according to Rose-Witebsky criteria. |
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| MeSH term(s) | Adolescent ; Adult ; Animals ; Autoantibodies/blood ; Autoantibodies/immunology ; Female ; Humans ; Immunization, Passive/methods ; Male ; Mice ; Mice, Inbred BALB C ; Middle Aged ; Myocarditis/blood ; Myocarditis/immunology ; Myocarditis/pathology ; Myocardium/immunology ; Myocardium/pathology ; Prospective Studies ; Species Specificity ; Young Adult |
| Chemical Substances | Autoantibodies |
| Language | English |
| Publishing date | 2015-01-20 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Randomized Controlled Trial |
| ZDB-ID | 779519-1 |
| ISSN | 1874-1754 ; 0167-5273 |
| ISSN (online) | 1874-1754 |
| ISSN | 0167-5273 |
| DOI | 10.1016/j.ijcard.2014.10.165 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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